Micropropagation and medicinal properties of Barleria greenii

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Page 1 and 2:
MICROPROPAGATION AND MEDICINAL PROP
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2.1.4 Stage II: Proliferation or mu
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Chapter 5 Pharmacological and phyto
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STUDENT DECLARATION Micropropagatio
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FACULTY OF SCIENCE & AGRICULTURE DE
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AMOO, S.O., FINNIE, J.F. and VAN ST
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LIST OF FIGURES Chapter 1 Figure 1.
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Figure 5.2: Anti-inflammatory activ
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LIST OF TABLES Chapter 2 Table 2.1:
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LIST OF ABBREVIATIONS [3G]BA 6-Benz
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PE Petroleum ether PEPC Phosphoenol
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status. Being a dwarf species, very
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activities shown by H. hystrix extr
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During this same period, these auth
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dark pink with magenta streaks on t
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traditional medicine (muthi) among
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1.5 Aims and objectives Endemic and
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2.1 Micropropagation 2.1.1 Introduc
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their constituent cells (MURASHIGE,
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media are sterile, the explants mai
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plants is higher (MURASHIGE, 1978)
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adventitious roots are produced wit
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DEBERGH and MAENE (1981) therefore
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cytokinins as well as these major e
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Auxins are known to play a crucial
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6-Benzyladenine (BA) 6-Furfurylamin
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STADEN and DREWES, 1992), the relat
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growth and development throughout t
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HASEGAWA et al (1973) reported a co
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Table 2.1 continued Plant species E
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2.2 Pharmacological and phytochemic
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filamentous fungi (such as Fusarium
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PGI2, PGF2α, are known to sensitiz
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Many researchers have reported anti
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inclusion of antioxidant and free r
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activity of gallotannins from Mangi
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3.1 Introduction Chapter 3 In vitro
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with 30 g l -1 sucrose, 0.1 g l -1
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andomised and maintained in a growt
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3.3.1 Explant decontamination Figur
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Adventitious shoots per explant (n)
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3.3.3 Effects of types and concentr
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Table 3.1: Effects of types and con
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3.3.4 Effects of photoperiod on sho
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to be a slow release reservoir of a
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Percentage survival 70 60 50 40 30
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exponential population growth rates
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Cultures were incubated under the s
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loosely closed screw cap jars (300
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Frequency of decontamination (%) 60
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Table 4.2: Frequencies of shoot, ro
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SALISBURY and ROSS (1992), a temper
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Table 4.5: Effects of temperature a
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photoperiod could therefore be due
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Titratable acidity (µM H + g -1 FW
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Figure 4.4: Huernia hystrix adventi
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strength MS medium with or without
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Chapter 5 Pharmacological and phyto
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Garden, Pietermaritzburg, South Afr
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2004) respectively, were also deter
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each of the plant extracts, the bla
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ORR =
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5.2.3.5 Gallotannin content The rho
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Barleria species, the EtOH extracts
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Table 5.2: Yield (% w/w) of extract
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Table 5.4: Antibacterial activity (
Page 133 and 134: Table 5.5: Antifungal activity of c
Page 135 and 136: 5.3.2.3 Anti-inflammatory activity
Page 137 and 138: Prostaglandin synthesis inhibition
Page 139 and 140: The AChE inhibitory activity of ext
Page 141 and 142: (A) DPPH Scavenging activity (%) (B
Page 143 and 144: Table 5.8: DPPH radical scavenging
Page 145 and 146: Absorbance 630 nm 2.2 2.0 1.8 1.6 1
Page 147 and 148: (ORHAN et al., 2009; ABDEL-HAMEED,
Page 149 and 150: 5.3.3 Phytochemical evaluation 5.3.
Page 151 and 152: 5.3.3.2 Total iridoid content Figur
Page 153 and 154: 5.3.3.3 Flavonoid content The flavo
Page 155 and 156: 5.3.3.4 Gallotannin content The roo
Page 157 and 158: 5.3.3.5 Condensed tannin (proanthoc
Page 159 and 160: Chapter 6 General conclusions An ef
Page 161 and 162: evaluating their safety may be nece
Page 163 and 164: ANAND, Y. and BANSAL, Y.K. 1998. Pl
Page 165 and 166: BASKARAN, P., VELAYUTHAM, P. and JA
Page 167 and 168: CHENG, S.H. and EDWARDS, G.E. 1991.
Page 169 and 170: DE KLERK, G.J., BRUGGE, J.T. and MA
Page 171 and 172: ETKIN, N.L. 1998. Indigenous patter
Page 173 and 174: GIRIDHAR, P., GURURAJ, H.B. and RAV
Page 175 and 176: HUANG, D., OU, B. and PRIOR, R.L. 2
Page 177 and 178: KIRANMAI, C., ARUNA, V., KARUPPUSAM
Page 179 and 180: LITZ, R.E. and CANOVER, R.A. 1981.
Page 181 and 182: MARKS, T.R. and SIMPSON, S.E. 1994.
Page 183: NDAWONDE, B.G., ZOBOLO, A.M., DLAMI
Page 187 and 188: RAI, M.K., AKHTAR, N. and JAISWAL,
Page 189 and 190: threatened plants - progress in the
Page 191 and 192: SRIVASTAVA, L.M. 2002. Plant Growth
Page 193 and 194: TURNER, S. 2001. Witwatersrand Nati
Page 195: WILLIAMSON, E., OKPAKO, D.T. and EV
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