Influence of Whisky Congeners on Health - The Institute of Brewing ...

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sorbitol in erythrocytes (nmol/mLRBC) glucose 5.5mM glucose 30mM glucose ellagic acid 1 mg/L glucose glucose glucose 30mM 30mM 30mM 30mM + + + + ellagic acid 3 mg/L ellagic acid 10 mg/L Epalrestat 3 mg/L Figure 10 Ellagic acid inhibits the accumulation <strong>of</strong> sorbitol in rerythrocytes Blood was collected into a heparinized tube from the abdominal aorta <strong>of</strong> the rats. Erythrocytes were obtained by centrifugation at 2000 xg and washed with cold saline twice. 1 ml <strong>of</strong> erythrocytes was added into 3 ml <strong>of</strong> Kreb’s-Ringer bicarbonate buffer, containing 30 mM glucose and each compound dissolved in 30 μL <strong>of</strong> dimethyl sulfoxide (DMSO), and was incubated at 37 ℃ in 5 % CO2 in air for 3 h. At the end <strong>of</strong> incubation periods, red blood cells were separated by centrifugation, washed, and precipitated with cold 6 % perchloric acid. The supernatant was neutralized with a same volume <strong>of</strong> 0.5 M Na3PO4 (pH 11). The sorbitol contents were measured enzymatically by the sorbitol dehydrogenase reaction. The amount <strong>of</strong> sorbitol is proportional to the formation <strong>of</strong> NADPH as measured by the increase in fluorescence under the excitation and emission at 355 and 460 nm, respectively.
own spirits <strong>Whisky</strong> A <strong>Whisky</strong> B <strong>Whisky</strong> C Brandy A Brandy B Brandy C Shochu A Shochu B Shochu C low Inhibiting activity kojic acid ( positive control) 0.00 0.01 0.02 0.03 0.04 0.05 1 / IC50 (ml / μg) Figure 11 Inhibitory effects <strong>of</strong> distilled liquors to tyrosinase activity High Tyrosinase source was prepared from murin B16 melanoma cells (Riken Cell Bank, Tsukuba, Japan). The cells were lysed by incubating at 4°C for 1hr in RIPA buffer (10 mM Tris-HCl, pH 7.5, 1 % NP-40, 0.1 % sodium deoxycholate, 0.1 % SDS, 150 mM NaCl, 1 mM EDTA, 0.5 mM AEBSF, and 10 μg/mL leupeptin). The lysate were centrifuged at 10,000g for 30 min to obtain the supernatant as the crude tyrosinase extract for the activity assay. The reaction mixture contained 50 mM phosphate buffer, pH 6.8, 0.05 % L-dopa and the supernatant (tyrosinase source). After incubation at 37°C for 20 min, dopachrome formation was monitored by measuring absorbance at wavelength 492 nm in a microplate reader.
Page 1 and 2: Influence
Page 3 and 4: Polyphenols exude from the oak cask
Page 5 and 6: Experimental Purine and carbohydrat
Page 7 and 8: time, and the samples were subjecte
Page 9 and 10: Figure 4 Influence
Page 11 and 12: whisky whisky congener liquid-liqui
Page 13 and 14: ppm. This level and its specific ac
Page 15 and 16: sorbitol in erythrocytes (nmol/mLRB
Page 17 and 18: 0.1 % SDS, 150 mM NaCl, 1 mM EDTA,
Page 19 and 20: Whisky and brandy
Page 21 and 22: Number of literatu
Page 23 and 24: serum uric acid level (%) ( %) 115
Page 25 and 26: a serum uric acid level (%) b 115 1
Page 27 and 28: purine body xanthine oxydase uric a
Page 29 and 30: whisky Evaporation and lyophilizati
Page 31: low low inhibiting inhibiting activ
Page 35: 6 white 5 ⊿ L* 4 3 2 1 0 -1 -2 UV

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