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Targeted differential display of abundantly expressed sequences ...

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73<br />

Fig. 3 A–E DNA <strong>sequences</strong><br />

<strong>of</strong> each <strong>of</strong> the five classes <strong>of</strong><br />

amplified cDNA fragments<br />

containing CBD-encoding regions<br />

(A–E), where A = cbhI.1,<br />

B = cbhI.2, C = ac1, D = ac2<br />

and E = cmc1. Differences<br />

between alleles are indicated by<br />

underlining and the alternative<br />

base is given beneath. Underlining<br />

<strong>of</strong> a base with a star (*)<br />

beneath indicates alternative<br />

positions at which the poly-A<br />

tail is added. The predicted<br />

amino-acid sequence is shown<br />

above the corresponding coding<br />

sequence (capital letters). The<br />

sequence in bold represents a<br />

sequence present in one allele<br />

<strong>of</strong> the cbhI.2 gene (B) which is<br />

absent in the other. Alternative<br />

stop codons in <strong>differential</strong>ly<br />

spliced transcripts <strong>of</strong> each allelic<br />

variant <strong>of</strong> cbhI.2 (B) are indicated<br />

in both bold and italics

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