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REED COLLEGE SCIENCE OUTREACH PROPERTIES OF MATTER

REED COLLEGE SCIENCE OUTREACH PROPERTIES OF MATTER

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28<br />

Chromatography Procedure<br />

1. After you hand out the pieces of filter paper, instruct the students to use a pencil to<br />

label one piece “water” and the other “alcohol.”<br />

2. Tape the top of the filter paper to a wooden dowel and lay the dowel across the<br />

mouth of the cup. Leave the strip of paper hanging in the cup.<br />

3. Now slowly pour water into the cup so that only the bottom edge of the strip is wet.<br />

Do not let the water cover any part of the dots of ink! Also be sure that the water does<br />

not splash onto the paper.<br />

4. Repeat the same procedure for the alcohol.<br />

5. Watch the water/alcohol spread up the paper until it is about an inch from the top of<br />

the paper. Take the paper out of the cup and lay it on a paper towel so that the ink<br />

stops spreading.<br />

6. Record the unique pattern for each of the known and mystery inks. Then compare<br />

the mystery ink’s pattern to those of the known inks. Can the students identify which<br />

ink was used for the mystery ink?<br />

Note: There will be different types of mystery ink used, so each group should have a<br />

different result.<br />

Wrap-up (10-15 Minutes):<br />

8. Once the students have cleaned up their workstations, have them compare their<br />

results with the class. Was every group able to identify their mystery ink?<br />

9. Give stickers to students for completed worksheets and be sure to check the Question<br />

Box for questions.

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