Guide to Ussing Chamber Systems - Harvard Apparatus

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Multichannel Ussing Chambers NAVICYTE & EASYMOUNT CHAMBER SYSTEMS Warner Instruments offers three multichannel Ussing chamber systems capable of supporting up to 24 independent chambers, all operating in parallel.Two systems position the membrane vertically and one positions the membrane horizontally. All three multichannel chamber systems embody the self-contained design and are fully capable of supporting either membranes or cell culture cups.The differences between these systems are explained below. NaviCyte Horizontal and Vertical Chamber Systems The Harvard/NaviCyte patented line of horizontal and vertical diffusion chambers is designed for the study of transport across cultured cell monolayers or excised tissue under dynamic conditions. The basic systemarchitecture shown here demonstrates the fundamental design of both the horizontal and vertical diffusion chamber systems. These systems are extremely well suited for non-electrical applications, however, the introduction of electrodes can provide the ability to make electrophysiological measurements. For larger applications, electrical manifolds can be employed to organize and simplify the connections to our various voltage/current clamps. NaviCyte chamber systems are manufactured from acrylic and can be temperature controlled. 66-0023 Electrode Diffusion Chamber Systems The NaviCyte Horizontal chamber system is designed for transport and toxicology studies using cells or tissue exposed to an air interface such as occurs in the in vivo environment (e.g., nasal, pulmonary, corneal, or dermal cells). The chambers create an environment wherein the apical surface can be exposed to liquids, semi-solid compounds, or to gases while the basolateral surface remains perfused with medium. NaviCyte Horizontal chambers accept either tissue mounting rings or Snapwell cell culture cups and can be used in either open or closed configurations. In the closed configuration, electrodes can be brought to bear to make electrophysiological membrane resistance measurements. Accepts from 1 to 6 chambers. Similar to the NaviCyte Horizontal, the NaviCyte Vertical chamber system is designed for transport studies on filter grown cell monolayers or excised tissue sections. This system differs, however, in that membranes are mounted vertically and the chamber blocks do not use inserts to secure tissues. Instead, tissue is affixed between the opposing faces of the chamber-halves using a series of pins surrounding the opening. Chambers are available with both circular and oblong openings, depending on the tissue type under study, and can also accommodate Snapwell TM culture cups. Several low volume chambers that reduce the amount of compound required to conduct permeability studies are also available. Accepts from 1 to 24 chambers. NaviCyte Electrodes The NaviCyte electrodes can be used with either the NaviCyte horizontal or vertical chamber systems and provide the ability to make electrophysiological recordings. Electrodes are terminated with a 2 mm pin connector and are compatible with the NaviCyte electric manifolds (used to connect chambers to our current/voltage clamps). The Ag/AgCl reference electrode is contained in a glass barrel that terminates in a micro-porous ceramic tip. The barrel is filled with a suitable electrolyte solution, usually the buffer used for the external media or 3M KCl, and is refillable. EasyMount Vertical Chamber Systems The EasyMount Vertical chamber systems are ideal for studies requiring electrophysiological measurement of transmembrane resistance. While visually similar to the NaviCyte Vertical chambers, EasyMount chambers are different in that they use inserts to position and secure tissues or culture cups within the chamber body. Inserts can be easily exchanged without removing the chamber from its support assembly or disturbing the electrodes. Chambers come in standard and low volume designs with a variety of inserts available for each. All chambers, however, mount into the same support assembly. Accepts from 2 to 8 chambers. Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com USSING CHAMBER SYSTEMS 8GUIDE TO
Multichannel Ussing Chamber NAVICYTE HORIZONTAL This system features the ability to run up to six chambers simultaneously and is optimized for transport studies on cells and tissues exposed to an air interface. The included heater block permits temperature control, with the use of a circulating water bath, for these chambers in both open and closed configurations. While optimized for diffusion-based studies, the use of optional electrodes permits electrophysiological membrane resistance measurements. Horizontal Diffusion Chamber Cross Section Horizontal Diffusion Chamber .567 cm2 The NaviCyte Horizontal chamber system consists of a heater/support block and up to six horizontal chambers. Chambers require the use of a supplied open or closed chamber cap into which either a Snapwell culture cup or a tissue mounting ring may be fitted. Culture cups or tissues are mounted on a horizontal plane. Chambers have basolateral and apical inputs for attachment to perfusion lines, and in the closed configuration chambers can accept electrodes. Single or Multichannel Setups Chambers can be used singly as a stand-alone entity, or can be mounted into the heater/support block in sets of up to six chambers per block. The heater/support block provides chamber heating and is recommended even if using a single chamber. Inserts are easily exchanged in mounted chambers. The compact footprint (38.1 x 8.9 x 10.2 cm) of the heater/support block conserves lab bench space. Open and Closed Configurations Open and closed chamber configurations are defined by the use of an open or closed chamber cap. Either cap completes the chamber and holds the culture cup or tissue ring into place. Both an open and closed cap is supplied with each chamber. In the open configuration, the upper surface (usually the apical side) of the membrane is directly accessible, making the system suitable for drug transport studies or cytotoxicity testing of liquids or semi-solid materials. In the closed configuration, the membrane can be exposed to solutions, exposed to gases, perfused with gas, or perfused with liquid. NaviCyte Electrodes and Manifolds While optimized for diffusion-based assays, NaviCyte electrodes are available for making electrophysiology-based measurements from NaviCyte chambers. Electrodes can only be used with the NaviCyte Horizontal chamber in its closed configuration. Electrical manifolds are available to organize the multiple connections between the amplifiers and the electrodes when makingelectrophysiology-based measurements using multiple chambers. Two electrical manifolds are available, one compatible with the VCC amplifiers and the other compatible with the ‘Computer Controlled Multi-Clamp with Software’ package. See the associated amplifiers for these required components. An optional gas manifold is also available to manage gas connections to the chambers. Note that a gas and electrical manifold cannot be used simultaneously with NaviCyte Horizontal system. Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com USSING CHAMBER SYSTEMS 9GUIDE TO
Page 1 and 2: Guide to Ussing Chamber Systems www
Page 3 and 4: Ussing Chambers Systems From Warner
Page 5 and 6: Classic Ussing Systems U9500 & U952
Page 7 and 8: Classic Ussing Systems U9500 & U952
Page 9: Self-Contained Ussing Chambers U992
Page 13 and 14: Multichannel Ussing Chamber NAVICYT
Page 19 and 20: Multichannel Ussing Chamber EASYMOU
Page 21 and 22: Single & Dual Channel Epithelial Vo
Page 23 and 24: Single Channel Epithelial Voltage C
Page 25 and 26: Multichannel Epithelial Voltage Cla
Page 27 and 28: Acquire & Analyze DATA ACQUISITION
Page 29 and 30: Data Acquisition & Analysis System
Page 31 and 32: Computer Controlled Multi-Clamp wit

Guide to Ussing Chamber Systems - Harvard Apparatus

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