Guide to Ussing Chamber Systems - Harvard Apparatus

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Multichannel Ussing Chamber EASYMOUNT The EasyMount chamber systems are ideal for studies requiring electrophysiological measurement of transmembrane resistance. While visually similar to the NaviCyte Vertical chambers, EasyMount chambers are different in that they use inserts to position and secure tissues or culture cups within the chamber body. Diffusion Chamber The EasyMount chamber systems consist of 2 to 8 vertical Ussing chambers, a heater block/support stand, needle valves for adjustment of gas flow (for oxygenation and gas lift stirring), and Ag/AgCl voltage/current electrodes for measuring transepithelial voltage and for passing current. Tissue specific inserts are ordered separately. Multichannel Setups All EasyMount chambers (P2300 and P2400,) use the same stands and electrodes. Stands are available to support 2, 4, 6, or 8 chambers and can be readily ganged to allow for higher count applications. Stands have an incorporated heater block that warms all chambers to the same temperature. A needle valve assembly (one set per chamber) is mounted on the rear of the heater block for regulation of the gas flow. GUIDE TO USSING CHAMBER SYSTEMS Inserts Electrodes EasyMount Chambers EasyMount chambers are a two piece assembly using an insert to secure and position tissues or culture cups. Tissues are placed in the chamber by loosening a thumbscrew, sliding the tissue holding insert into the space between the chamber halves, and retightening the thumbscrew. Chambers do not need to be removed from the heater block, nor are the electrodes disturbed, when placing an insert. This easy and rapid replacement process makes the EasyMount system an excellent choice for high-throughput applications. Chambers are available in two styles; the P2300 is the standard chamber and accommodates a large variety of inserts and the P2400 is designed for low-volume applications. The P2300 has chamber caps available for sealing the chamber when radioligands or toxic chemicals are used. EasyMount Inserts EasyMount inserts are available to mount a variety of tissues and can also accommodate cell culture cups. Each chamber type has its own supporting family of inserts. The P2300 Series inserts are available to accommodate Snapwell, Millicell, Nunc, and Transwell culture cups. Inserts are also available with pins or o-rings in both oblong and round formats. P2400 Series inserts do not support culture cups but are available with pins or o-rings in both oblong and round formats. Electrodes and Accessories Electrodes are available in sets comprised of two sintered Ag/AgCl pellet electrodes for voltage sensing (black), two Ag wire electrodes for current passing (white), and eight electrodes tips. The number of sets provided is dependent on the system purchased, however, one electrode set is required for each chamber. Replacement current and voltage electrodes are available, as are electrode tips. Additional accessories include electrode lead sets and chamber filling needles. Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com 16
Multichannel Ussing Chamber EASYMOUNT (continued) How to Order: • Select Chamber Assembly. Each assembly includes heater block/stand, chambers, needle valves, and electrodes. • Select Chamber Inserts. Select from P2300 or P2400 Series depending on volume of system assembly chosen. • Select FN15 filling needles and extra electrode tips. • Select P23000 Chamber Caps (if using radioligands or toxic chemicals with these chambers) EasyMount Chamber Assembly Order # Model Description P2300 Chamber Systems, No Inserts (Standard Volume) 69-1070 CSYS-2HA P2300 EasyMount system with 2 chambers 69-1071 CSYS-4HA P2300 EasyMount system with 4 chambers 69-1072 CSYS-6HA P2300 EasyMount system with 6 chambers 69-1073 CSYS-8HA P2300 EasyMount system with 8 chambers P2400 Chamber Systems, No Inserts (Low Volume) 69-1074 LVSYS-2HA P2400 EasyMount system with 2 chambers 69-1075 LVSYS-4HA P2400 EasyMount system with 4 chambers 69-1076 LVSYS-6HA P2400 EasyMount system with 6 chambers 69-1077 LVSYS-8HA P2400 EasyMount system with 8 chambers Chamber Inserts Order # Model Aperture Working Area Description Fits P2300 Chamber Systems 69-0956 P2302 12 mm round 1.12 cm 2 For Snapwell cell culture cups 69-1081 P2302M 8.75 mm round 0.60 cm 2 For Millicell cell culture cups 69-1082 P2302N 8.0 mm round 0.50 cm 2 For Nunc/Anopore cell culture cups 69-1083 P2302T 6.5 mm round 0.33 cm 2 For Costar Transwell cell culture cups 69-1084 P2303 2.8 x 4.5 mm 0.10 cm 2 For juvenile mouse intestine 69-1085 P2303A 2.8 x 4.5 mm 0.10 cm 2 For juvenile mouse intestine or bladder, with pins 69-0957 P2304 2.8 x 11.0 mm 0.30 cm 2 For mouse intestine, with pins 69-0958 P2305 4.5 x 11.2 mm 0.50 cm 2 For rat and larger animal intestine, with pins 69-0959 P2306 2.8 x 1.5 mm 0.036 cm 2 For mouse trachea and similar tissue 69-0960 P2307 2.0 mm round 0.031 cm 2 For small tissues (e.g., biopsies) 69-1086 P2308 1.0 mm round 0.008 cm 2 For very small, thin tissues (e.g., lower mouse trachea) 69-0961 P2310 5.0 mm round 0.20 cm 2 With pins 69-0962 P2311 6.2 mm round 0.30 cm 2 With pins 69-1087 P2311A 5.7 mm round 0.26 cm 2 With pins 69-0963 P2312 8.0 mm round 0.50 cm 2 With pins 69-0964 P2313 9.5 mm round 0.71 cm 2 With pins 69-0965 P2314 11.3 mm round 1.00 cm 2 With pins 69-0966 P2315 12.7 mm round 1.26 cm 2 With pins 69-1088 P2316 5.0 mm round 0.20 cm 2 For skin, with o-ring 69-1089 P2317 10.8 mm round 0.93 cm 2 For synthetic membranes, with o-ring 69-1090 P2318 11.3 mm round 1.00 cm 2 For frog skin, with o-ring 69-1091 P2319 12.7 mm round 1.26 cm 2 For studying mucus layer, with spacer ring Fits P2400 Chamber Systems 69-1092 P2403 2.8 x 4.5 mm 0.10 cm 2 For small tissue specimens 69-0969 P2404 2.8 x 9.2 mm 0.25 cm 2 For mouse intestine 69-0970 P2405 4.5 x 9.2 mm 0.40 cm 2 For rat and larger animal intestine 69-0971 P2406 2.8 x 1.5 mm 0.04 cm 2 For mouse trachea 69-0972 P2407 2.0 mm round 0.031 cm 2 For biopsies 69-1093 P2408 0.8 mm round 0.005 cm 2 For biopsies 69-1094 P2408A 0.5 mm round 0.002 cm 2 For biopsies 69-1095 P2408B 1.0 mm round 0.008 cm 2 For biopsies 69-0973 P2410 5.0 mm round 0.20 cm 2 With pins 69-0975 P2412 8.0 mm round 0.50 cm 2 With pins 69-0976 P2413 9.5 mm round 0.71 cm 2 With pins GUIDE TO USSING CHAMBER SYSTEMS Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com 17
Page 1 and 2: Guide to Ussing Chamber Systems www
Page 3 and 4: Ussing Chambers Systems From Warner
Page 5 and 6: Classic Ussing Systems U9500 & U952
Page 7 and 8: Classic Ussing Systems U9500 & U952
Page 9 and 10: Self-Contained Ussing Chambers U992
Page 11 and 12: Multichannel Ussing Chamber NAVICYT
Page 17: Multichannel Ussing Chamber NAVICYT
Page 21 and 22: Single & Dual Channel Epithelial Vo
Page 23 and 24: Single Channel Epithelial Voltage C
Page 25 and 26: Multichannel Epithelial Voltage Cla
Page 27 and 28: Acquire & Analyze DATA ACQUISITION
Page 29 and 30: Data Acquisition & Analysis System
Page 31 and 32: Computer Controlled Multi-Clamp wit

Guide to Ussing Chamber Systems - Harvard Apparatus

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