Guide to Ussing Chamber Systems - Harvard Apparatus

Acquire & Analyze
DATA ACQUISITION & ANALYSIS SOFTWARE
Acquire & Analyze is a comprehensive data acquisition and analysis package designed specifically for recording
transepithelial electrophysiological data from epithelia mounted in Ussing chambers. The system consists of data acquisition
hardware and software used to record current, voltage and conductance/resistance from up to 8 tissues as well as analysis
software that enables one to quickly extract selected data and output it to summary spreadsheets.
Unlike general-purpose data acquisition packages, Acquire & Analyze is
written specifically for the study of epithelial tissues.
For example, say you want to measure the dose/response curve of an
agent on the I sc and G t by progressive additions in eight tissue samples.
To do this you monitor the I sc and G t on the computer until stable basal
readings are attained. You then place an event mark in the file and make
the first addition to all 8 tissues. When the current reaches a new stable
level (or a specified time has passed) you place another event mark and
make the second addition. This process is repeated until all additions
have been made.
To summarize the effect of the additions, use Analyze to graph the data
for all 8 tissues. Begin by displaying the movable data bar and
positioning it over the basal currents just to the left of the first event mark.
Right-click the mouse to save the mean values of current, conductance
and voltage to a spreadsheet. Simply repeat the mouse movements and
clicks to obtain the data for each subsequent addition. The spreadsheet
thus created can be saved and imported directly into Excel for further
analysis.
Data Acquisition and Analysis Capabilities
Acquire & Analyze runs under Windows and includes a data acquisition
card, input/output cables, and software. The system can measure
currents and voltages from up to 8 voltage-current clamp channels.
• Activate/deactivate acquisition on individual clamp units
• Setup and toggle between fast and slow acquisition
speeds to capture transient events; speeds can be set by
user and can be changed at any time during experiment
• Place time marks into file to indicate when experimental
manipulations (e.g., drug additions) have been made;
marks can be predefined and selected from menu or be
entered from keyboard
• Graphically display current, voltage, conductance,
resisance, calculated open circuit voltage and calculated
I sc during data acquisition in multiple formats
• Graphical/digital readout of current, voltage or
conductance vs. time
• Plot raw or area corrected data
• User definable x, y scaling; zoom all, in, out, or previous;
pan left, right, up or down
• Extract data under movable, adjustable width time bar in
file format compatible with spreadsheet files
• Extracted data may be individual points or mean values
• Extracted data may be imported into commercial graphics
packages to produce publication quality graphs
• Linear regression analysis of conductance vs. current can
be performed on data within time bar to estimate
pericellular pathway conductance
Order # Model Description
69-3145 Acquire & Analyze II Data acquisition and
analysis software
69-0954 Mod 08/A Interface cable for VCC
600 and VCC MC2 amps
69-1005 DB9EC800 Interface cable for
EC-800 amp
69-1009 DB15EC825 Interface cable for
EC-825A amp
GUIDE TO USSING CHAMBER SYSTEMS
Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com
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