Guide to Ussing Chamber Systems - Harvard Apparatus

Computer Controlled Multi-Clamp with Software
DATA ACQUISITION & ANALYSIS SYSTEM
This unique package integrates the amplifiers, data acquisition system, and analysis software all into one convenient box!
The system provides individual data acquisition and control for 2, 4 or 6 chambers and is fully compatible with the NaviCyte
horizontal/vertical diffusion chamber systems. Windows 95/98/2000/XP compatible.
Software Features Includes:
• Automatic compensation of fluid resistance and electrode
potential during the experiment
• Individual voltage clamp, current clamp or open circuit
control for each chamber
• Overlay of uni- or bipolar current pulses with variable
parameters for dynamic definition of G t /R t
• I/V and V/I curves with freely adjustable parameters
• Minimum pulse duration 200 ms, max. amplitude
±250 µA for dynamic definition of G t /R t
• Ability to input marker and comments and save with
data file
• Printout of parameters in variable timetable
• Data format compatible to Excel
• Continuous graphics display
• Experiment time up to 8 hours
GUIDE TO USSING CHAMBER SYSTEMS
The Computer Controlled Multi-Clamp with Software is a combined
voltage/current clamp and data acquisition system in one compact
microcomputer controlled unit. In its full configuration it can be used to
control and collect electrophysiological data from up to six NaviCyte
horizontal or vertical diffusion chambers.
The system provides independent chamber control in three different
modes: voltage clamp, current clamp, and open circuit. An change
between these modes is possible during the experiment at each time point.
The Multi-Clamp is available in 2-, 4- or 6-channel configurations and is
fully compatible with both NaviCyte Ussing chamber systems. The clamp
provides a separate A/D converter and isolated current source for each
chamber, and current and voltage electrodes from each half-cell are
connected to the Multi-Clamp via an electrode manifold. Data exchange
between the PC and the Multi-Clamp is via standard COM ports, and
depending on the number of ports available, a single PC can handle
several clamps.
An interesting option consists of the incorporation of a gas delivery
system into a NaviCyte/Multi-Clamp system. This gas delivery system
consists of 12 precision gas flow meters mounted to a stabilizing base
and connect to a 6-channel NaviCyte vertical or horizontal chamber
systems. The 600 x 400 mm stabilizing base also houses an electrode
manifold for connecting each Ag/AgCl electrodes to their respective
half-cells.
Software
The Multi-Clamp system comes complete with an easy to use Windows
95/98/2000/XP based control and data acquisition software package
that allows for the individual control and monitoring of each chamber.
Specifications
Channels 2, 4 or 6
A/D Subsystem for
Analog Data Acquisition 13 Bit
Current Source by D/A 12 Bit, isolated
Serial Connection to PC COM1/COM2 for bi-directional
data transfer
Case Dimensions
24.5 x 11.4 x 19.7 cm
(9.6 x 4.5 x 7.8 in)
Power
100/230 VAC, 50/60 Hz
Input Resistance
> 10 9 Ω differential
Input Current
< 100 pA
Input Voltage
±400 mV, 0.1 mV step
Current Output
±2000 µA, 1 µA step
Product of Current and
External Resistance Max 13 V
Order # Description
69-0300 2-Channel voltage/current clamp system
with software
69-0301 4-Channel voltage/current clamp system
with software
69-0302 6-Channel voltage/current clamp system
with software
66-0025 6-Channel electrode manifold for NaviCyte
chamber systems
Perfusion and Electrical Accessories
69-0303 12-Channel precision flow-meter and
electrode manifold
66-0025 6-channel electrical manifold for NaviCyte
chamber systems
Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1278 • www.warneronline.com
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