Presentation_Grau(Roche)_Miptec 2011_ ... - Cisbio Bioassays

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Introduction TagLite Results Outlook Sandra Grau, Roche Glycart AG 10
TagLite – results FcgRIIIaV158 and F158 competition assay Tb FRET signal Tb Unlabeled IgGs with various a-fuc degree Human IgG-d2 low a-fuc Hek Hek Assay setup: •10.000 cells per well (expressing huFcgRIIIaV158 or F158 labeled with Tb) •IgGs with various a-fucosylation degrees (4, 8, 11, 57 and 80 %) conc. 4000 to 1.8 nM final in well (1:3 dilutions). •Labeled IgGs (human IgG-d2); conc. 50 nM final in well •Incubation time: 0h, 1h, 3h, 5h @ RT Sandra Grau, Roche Glycart AG
Page 1 and 2: IgG core a-fucosylation and its imp
Page 3 and 4: Introduction Antibody therapies ‣
Page 5 and 6: Introduction Glycoengineering ‣ G
Page 7 and 8: Introduction Crystal structure of F
Page 9: Introduction Characterization of mo
Page 13 and 14: TagLite - results FcgRIIIa(F158)
Page 15 and 16: a-fuc increase a-fuc increase TagLi
Page 17 and 18: Introduction TagLite Results Outloo
Page 19 and 20: Acknowledgements Roche Glycart Pabl

Presentation_Grau(Roche)_Miptec 2011_ ... - Cisbio Bioassays

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