Presentation_Grau(Roche)_Miptec 2011_ ... - Cisbio Bioassays

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TagLite – results Comparison FcgRIIIa(V158) and FcgRIIIa(F158) Sandra Grau, Roche Glycart AG/ Delphine Jaga, Cisbio Bioassay 14
a-fuc increase a-fuc increase TagLite – results Correlation with other methods Biacore binding curve – capture format huFcgRIIIaF158 MALDI Biacore TagLite 4 % a-fuc FcgRIIIaF158 a-fuc (%) KD (nM) EC50 (nM) 832 4 1300 881 834 8 940 728 835 11 845 170 836 57 68 32 842 80 39 27 improved off rate 80 % a-fuc FcgRIIIaF158 improve in KD/EC50 improve in KD/EC50 huFcgRIIIaV158 MALDI Biacore TagLite a-fuc (%) KD (nM) EC50 (nM) 832 4 200 555 834 8 201 370 835 11 111 226 836 57 17 28 842 80 7 13 4 % a-fuc FcgRIIIaV158 Note: KD values (Biacore) depend a lot on the format used. In the present format KD values for a-fuc abs tend to be lower than with other formats. You can expect values ranging from 500-700 nM for FcgRIIIaV158 and low a-fuc abs. improved off rate 80 % a-fuc FcgRIIIaV158 Sandra Grau, Roche Glycart AG/ Delphine Jaga, Cisbio Bioassay 15
Page 1 and 2: IgG core a-fucosylation and its imp
Page 3 and 4: Introduction Antibody therapies ‣
Page 5 and 6: Introduction Glycoengineering ‣ G
Page 7 and 8: Introduction Crystal structure of F
Page 9 and 10: Introduction Characterization of mo
Page 11 and 12: TagLite - results FcgRIIIaV158 and
Page 13: TagLite - results FcgRIIIa(F158)
Page 17 and 18: Introduction TagLite Results Outloo
Page 19 and 20: Acknowledgements Roche Glycart Pabl

Presentation_Grau(Roche)_Miptec 2011_ ... - Cisbio Bioassays

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