Ex vivo modeling of HIV persistence in successfully treated subjects ...
Ex vivo modeling of HIV persistence in successfully treated subjects ...
Ex vivo modeling of HIV persistence in successfully treated subjects ...
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<strong>HIV</strong> Persistence Detection Assay<br />
“HPDA”<br />
• 5.10 6 CD4 T cells from virally suppressed <strong>subjects</strong><br />
cultured <strong>in</strong> 1mL <strong>of</strong> RPMI 10% FBS + AZT, RAL, EFV<br />
• Harvest half the medium at day 3 and 6<br />
• vRNA RT-qPCR on ultracentifuged supernatants<br />
ARVs<br />
CD4 T cells from virally<br />
suppressed <strong>HIV</strong> <strong>in</strong>fected <strong>subjects</strong><br />
Quantification by RT-qPCR with<br />
a sensitivity <strong>of</strong> 1 <strong>HIV</strong> RNA copy
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