Ex vivo modeling of HIV persistence in successfully treated subjects ...
Ex vivo modeling of HIV persistence in successfully treated subjects ...
Ex vivo modeling of HIV persistence in successfully treated subjects ...
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HPDA: Measur<strong>in</strong>g the <strong>HIV</strong> reservoir size<br />
Assay<br />
qPCR<br />
<strong>HIV</strong> reservoir measured by<br />
Number <strong>of</strong> events<br />
Integrated <strong>HIV</strong> DNA<br />
Pros and cons<br />
+ : Easy, fast, sensitive<br />
- : The majority <strong>of</strong> <strong>in</strong>tegrated<br />
DNA is defective<br />
<strong>HIV</strong> Persistence Detection Assay<br />
ARV<br />
Production competent virus<br />
+ : Relatively easy and fast<br />
+ : Useful to test reactivation<br />
agents<br />
+ : Measure the frequency <strong>of</strong><br />
cells directly targeted by<br />
reactivation strategies<br />
co-culture assay<br />
Allogenic cells<br />
Replication competent virus<br />
- : Laborious and costly<br />
+ : Measure the frequency <strong>of</strong> cells<br />
able to produce <strong>in</strong>fectious particles<br />
responsible for viral rebound at<br />
ART <strong>in</strong>terruption.<br />
+ : Readout for sterilized cure<br />
Cost, labor
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