Ex vivo modeling of HIV persistence in successfully treated subjects ...

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HPDA: Measuring the HIV reservoir size Assay qPCR HIV reservoir measured by Number of events Integrated HIV DNA Pros and cons + : Easy, fast, sensitive - : The majority of integrated DNA is defective HIV Persistence Detection Assay ARV Production competent virus + : Relatively easy and fast + : Useful to test reactivation agents + : Measure the frequency of cells directly targeted by reactivation strategies co-culture assay Allogenic cells Replication competent virus - : Laborious and costly + : Measure the frequency of cells able to produce infectious particles responsible for viral rebound at ART interruption. + : Readout for sterilized cure Cost, labor
HPDA: Identifying reactivation strategies Reactivation (% relative to CD3/CD28) 40 30 20 10 0 n=3 Known anti-latency agents: PKC activators : prostratin, bryostatin HDAC inhibitors : SAHA, VPA The HPDA allows the evaluation of the ability of anti-latency agents to reactivate virus in latently infected cells from virally suppressed subjects.
Page 1 and 2: “Towards an HIV Cure” Pre-Confe
Page 3 and 4: • Current methods to study HIV la
Page 5 and 6: HPDA: Measuring the HIV reservoir s
Page 7: HPDA: Measuring the HIV reservoir s
Page 11 and 12: HPDA: Identifying novel antiviral c
Page 13 and 14: Conclusions • Therapeutic strateg
Page 15: Acknowledgement Volunteers Claire V

Ex vivo modeling of HIV persistence in successfully treated subjects ...

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