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Molecular Tools 

bioaffinitymatrix 

proteomics 

biokits&systems 

buffers&bioreagents 

enzymes&substrates 

biochemicals 

biomedia 

customlabservices 

Excellent Service 

Lower Prices 

Superior Quality 

Source of 

molecular tools and 

bioreagents 

1.888.bio.PLUS 

www.bio-WORLD.com 

save@bio-WORLD.com 

50 

proteomics activeligands > liveproteins

Contact Information 

4150 Tuller Road, Suite 228 

PO Box 888 

Dublin, OH 43017 

USA 

toll free: 1.800.860.9729 

P: 614.792.8680 

F: 614.792.8685 


save@bio-WORLD.com 

bioWORLD, a division of Gene Linx International, 

Inc., was established in October 1993 with the mission 

to bring Quality, Low Cost molecular tools, 

biological reagents and lab supplies for analytical, 

educational, and research institutions around the 

world. We specialize in products for the 

Life Sciences and Biotechnology industries with 

focus on bioAffinity matrices for the proteomics 

and signal transduction research. 

The company headquarters are based in Dublin, 

Ohio with sales offices located across the globe . 

Through manufacture and international acquisitions, 

bioWorld supplies products for biomedical 

and life sciences industries at highly competitive 

prices. In addition, we provide custom lab services 

and formulations for the biotechnology industry. 

Save 

on hundreds of new products 

biokits 

biomedia 

biologicals 

bioreagents 

biopolymers 

biochemicals 

biodetergents 

biomolecularservices 

Serving life sciences World-wide

1. bioaffinitymatrix 

Affinity Chromatography 3 

MagneTALON 20 

Coated Microtiter Plates 23 

Lectin Affinity 26 

5-6. enzymes&substrates • recombinantproteins 

Enzymes 88 

Substrates 89 

Recombinant Proteins 91 

Antibodies 92 

2. proteomics 

Protein Extraction 29 

Protein Quantification 30 

Protein Purification 32 

Protein Linkers 41 

Protein Electrophoresis 45 

Mass Spectrometry 50 

3. biokits&systems 

Bio Kits 53 

Gene Kits 56 

4. buffers&reagents 

DNA/RNA/Hybridization 

Electrophoresis 

Molecular Biology 

Immunology 

67 

68 

71 

7. biochemicals 

8. biomedia 

77 10. Index 

Antibiotics 96 

Detergents 105 

Research Chemicals 108 

Animal Media 122 

Microbiological Media 126 

Plant Media 128 

9. customlabservices 

Affinity Chromatography 131 

Custom Peptide Synthesis 132 

Antibody Production 135 

2-D Gel Analysis 137 

Mass Spectrometry 138 

Recombinant Technologies 139 

> 

accelerate: lifesciencesresearch

AffinityPurification: 

Affinity Chromatography 

Affinity purification is the process where a protein is purified based on its 

inherent specific binding properties to an immobilized ligand on a solid matrix 

support. The affinity chromatography procedure was established in 1967 

(Axen R., Proth J., Embark S.), which is based on the principle of reversible 

formation of a tight binding complex between a protein and an immobilized 

ligand. Based on specificity, the protein of interest binds to the immobilized 

ligand. Impurities pass through the affinity column because they lack 

the ability to bind to the ligand. The bound protein can then be eluted using 

a competitive ligand or protein configuration altering reagent. The specific 

binding between the protein and immobilized ligand allow up to a 10,000-fold 

purification of a protein from a crude mixture. 

Several different matrices may be used for affinity chromatography. Crosslinked 

gel supports are used for gravity column, spin column and batch-scale 

purifications. Silica or porous beads are used for High Performance Liquid 

Chromatography (HPLC). Coated microplates are used for high throughput 

screenings. Also, magnetic particles have recently been useful in affinity 

purification. 

Researchers can purchase all the materials necessary for affinity purifications 

of recombinant or natural proteins from bioWORLD. We offer a variety 

of ligands coupled to our own agarose formulation, Sepharopore, as well as 

coated microplates and magnetic beads offered in two forms: BlackPearl & 

MagneTalon. 

All our bioaffintiy matrix products are guaranteed to perform accordingly and 

are competitively priced to reduce your research costs. 

The goal of affinity chromatography is to separate all the molecules of a particular specificity 

from a mixture of proteins such as blood serum. For example, the antibodies in a serum 

sample specific for a particular antigenic determinant can be isolated by the use of affinity 

chromatography. The process can be performed in the following steps: 

1. A bioAffinity matrix (immunoadsorbent), to which the peptide or protein antigen (shown in 

red) has been covalently coupled is loaded into the column. Agarose, sephadex, derivatives 

of cellulose or other polymers can be used as the matrix. 

2. The serum is passed over the bioAffinity matrix. Those antibodies in the mixture specific 

for the antigen (shown in blue) noncovalently bind and are retained. Antibodies of other 

specificities (green) and other serum proteins (yellow) pass through the column. 

3. Following a thorough wash, a reagent is passed through the column to release the antibodies 

from the bioAffinity matrix. A denaturing agent, such as 8M urea or SDS can also be 

used to break the interaction by altering the configuration of the antigen-binding site of the 

antibody molecule. 

Another approach is to elute with a soluble form of the antigen. These molecules compete 

with the immunoadsorbent for the antigen-binding sites of the antibodies and release the 

pure antibodies to be collected for further use. 

 

 

 

 

Substances Isolated by Affinity Chromatography and the 

Complementary Ligands: 

 

Substance Isolated by 


Ligand 

 

 

 

 

 

Enzyme 

Antibody 

Substrate, cofactor 

Antigen, Virus, Cell 

Polysaccharide, 

glycoprotein Lectin 

 

 

 

 

Nucleic acid binding 

protein Nucleic Acid 

Hormone receptor 

Hormone 

activeligands > liveproteins SM

ioaffinitymatrix 

1 


Alginate & Chitin Beads 

Cellulose Matrix 

MagneTALON (Magnetic Beads) 

Coated Microtiter Plates 

bioLAM (Lectin Affinity Matrix) 



F: 614.792.8685 

save@bio-world.com 

> 

accelerate: proteinpurification 

3


bioAgarose Beads 

Save 

30% 

Affinity Chromatography bioaffinitymatrix 

Sepharopore 4B 

A high gel strength bioagarose matrix for gel filtration 

and coupling of affinity ligands suitable for 

analytical and preparative protein purifications. 

Equal to Sepharose 4B 

• Particle size (range): 50-170µm 

• Molecular Weight range: 6x10 4 - 2x10 7 

• Matrix: Agarose, 4%Beads 

• pH stability: 4-9 

• Flow specifications: 70-140cm/h 

• Supplied as a slurry in 20% ethanol 

Sepharopore 4B-MB 

A high gel strength bioagarose fast flow macro 

bead matrix for gel filtration and coupling of affinity 

ligands suitable for analytical and preparative 

protein purifications. 

Similar to Sepharose 4B-MB 

• Particle size (range): 175-400µm 


• Matrix: Agarose, 4% Beads 



• Supplied as a slurry in 20% ethanol 

#506421 100ml $ 68.60 

#506422 500ml $ 214.00 

#506423 1L $ 398.00 

New 

New 

#506439 100ml $ 64.00 

#506440 500ml $ 198.00 

#506441 1L $ 365.00 

Sepharopore 6B 

An agarose medium for gel filtration and coupling 

of affinity ligands. Low nonspecific interactions and 

high yields. Provides excellent separation over a 

wide molecular weight range. 

Equal to Sepharose 6B 

• Particle size range: 50-170 µm 

• Molecular Weight range: 1x10 4 – 4x10 6 




• Supplied as slurry in 20% ethanol 

Sepharopore 6B-MB 

An agarose medium for gel filtration and coupling 

of affinity ligands. A hydrophilic backbone results 

in low nonspecific interactions and high yields. 

Provides excellent separation over a wide molecular 

weight range. 

Similar to Sepharose 6B-MB 

• Particle size 175-400µm 






New 

New 

#506436 100ml $ 72.50 

#506437 500ml $ 217.50 

#506438 1L $ 399.00 

#506424 100ml $ 69.00 

#506425 500ml $ 214.00 

#506426 1L $ 386.00 

4 

Sepharopore 4B-CL 

A cross-linked bioagarose medium for gel filtration 

and coupling of affinity ligands. Hydrophilic backbone 

results in low nonspecific interactions and high 

recoveries of proteins. Provides excellent separation 

over a wide molecular weight range. 

Equal to Sepharose 4B-CL 

• Particle size (range): 50-175 µm 


• Matrix: Agarose, 4% cross-linked Beads 



New 

#506433 100ml $ 74.00 

#506434 500ml $ 215.00 

#506435 1L $ 417.00 

Sepharopore 6B-CL 

A cross-linked high strength agarose medium for 

gel filtration and coupling of affinity ligands. A 

#506455 100ml $ 76.60 

hydrophilic backbone results in low nonspecific interactions 

#506456 500ml $ 234.00 

and high yields. Excellent separation over 

a wide molecular weight range. 

#506457 1L $ 417.00 

Equal to Sepharose 6B-CL 

• Particle Size range: 50-170 µm 






New

ioAlginate Beads (BAB) 

Alginate is a biopolymer commonly used for immobilization of cells, enzymes and encapsulation applications. Due to its characteristic structure, 

alginate solution can be rapidly cured by action mainly of bivalent ions. The special curing gives the resulting hydrogel beads with a 

characteristic behavior dependent upon conditions of pH, temperature or contents of chelate forming agents or special ions. Cu-Alginate has 

been used for immobilized metal-affinity chromatography purification of IgG. 

• Microbeads: 100-250µm. Used for protein fractionation & affinity chromatography 

• Macrobeads: 200-500µm. Used for enzymes & cell immobilization 

New 

bioAlginate Beads (BAB) 

Micro 

• Alginate Micro beads 

Macro 

• Alginate Macro beads 

Co2+ -Alginate (Co-BAB) 

Micro 

• Cobalt charged 

Alginate Micro beads 

#506353 25ml $ 39.00 

#506354 100ml $ 87.00 

#506355 250ml $ 185.00 

#506356 25ml $ 37.00 

#506357 100ml $ 85.00 

#506358 250ml $ 178.00 

#506969 25ml $ 47.00 

#506970 100ml $ 100.00 

#506371 250ml $ 250.00 

New 

Ni2+ -Alginate (Ni-BAB) 

Micro 

• Nickel charged 


Macro 

• Nickel charged 

Alginate Macro beads 

Cu2+ -Alginate (Cu-BAB) 

Micro 

• Copper charged 


Save 

30% 

#506362 25ml $ 47.00 

#506363 100ml $ 100.00 

#506364 250ml $ 250.00 

#506371 25ml $ 39.50 

#506372 100ml $ 98.00 

#506373 250ml $ 197.00 

#506359 25ml $ 47.00 

#506360 100ml $ 100.00 

#506361 250ml $ 250.00 

bioaffinitymatrix Affinity Chromatography 

Macro 

• Cobalt charged 


#506365 25ml $ 39.50 

#506366 100ml $ 88.00 

Macro 

• Copper charged 


#506368 25ml $ 39.50 

#506369 100ml $ 88.00 

New 

#506367 250ml $ 187.00 

New 

#506370 250ml $ 187.00 



F: 614.792.8685 


Custom coupling and encapsulations available (call 1•888•bio•PLUS for pricing and availability) 

5

Polystyrene Beads 


Polystyrene (PS) and styrene/divinylbenzene copolymer (S/DVB), plain (un-dyed), hydrophobic microspheres with sulfate 

surface groups are used for many applications. They are used as size standards for instrument calibration. Polystyrene 

beads can be coated with proteins for a broad range of diagnostic applications such as agglutination, lateral flow and 

other sophisticated immunoassays. 

• bioWorld provides custom ligation /activation of polystyrene beads with any ligand and chemistry 

applicable (please inquire). 

• Bead Size (range): 15-25 µm 

• Suitable for high pressure chromatography. 

• For activated polystyrene beads please call 1-888-bioPLUS. 

bioSilica Beads 

Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, density 1.96 

g/cm 3 , refractive index (589 nm) ~ 1.37. Silica beads have high temperature stability. Because hydrophilic silica does not 

adsorb proteins, silica microspheres are used in immunoassays where a low nonspecific protein binding is needed. These 

beads are used to adsorb DNA, RNA and oligonucoeotides. They can be used for many chromatography applications. 

• bioWorld provides custom activation and ligation of bioSilica beads (please inquire). 

• For activated silica-coated magnetic beads see page 20 

BlackPearl Magnetic Beads 

#506374 1 ml $ 98.00 

#506375 5 ml $ 272.00 

#506376 15 ml $ 498.00 

#506352 25ml $ 697.00 

#506377 5 ml $ 84.00 

#506378 10 ml $ 135.00 

#506379 15 ml $ 184.00 

Utilized in magnetic separation of cells, organelles, proteins, immunoglobins, nucleic acids, and many other types of 

molecules in biological and non-biological systems. BlackPearl beads are made with high gel strength agarose and 

magnetic particles. Magnetic beads substantially shorten each step of purification because of their easy magnetic retrieval. 

Exposure to sample, washing of unbound samples and washing the target components free of the ligand on the 

bead are all made easier and quicker. Magnetic bioseparation can also avoid unnecessary dilution introduced by typical 

column elution procedures. 

#506453 5ml $ 69.00 

#506458 25ml $ 198.00 

#506459 50ml $ 347.60 





• Storage: 2-8°C 

• For activated BlackPearl Magnetic beads see page 9. 

See Page 20-21 for 

MagneTalons 

Magnetic 

6 

magneticbioseparations 

New 

Bioseparations


Activated Sepharose 

CNBr-Activated Sepharose 

Preactivated matrix for immobilization of ligands containing primary amines. Simple, reproducible and reliable 

method for covalent immobilization of ligands. Mild coupling condition makes it an ideal coupling method for enzymes, 

antibodies and proteins. A popular choice for the immobilization of large proteins and nucleic acid ligands. Supplied as 

lyophilized powder containing stabilizers. 1g swells to 3-4ml gel. 

• Group to be coupled: -NH 2 

• Chemical linkage of ligand: Isourea 

• Matrix: Agarose Beads, 4% 

• MW exclusion limit: Approx. 2 x 10 7 

• Particle size /range: 45-165µm 

CDI-Activated Sepharose 

Preactivated matrix for immobilization of ligands containing primary amines. N, N’-Carbonyl diimidazole (CDI)- 

mediated activation of the Sepharose matrix allows for coupling of the primary amino ligands by stable amidebond 

formation. No charge is introduced into the matrix after the coupling reaction. Minimal ligand leakage occurs 

after coupling. The activated matrix is much more resistant to hydrolysis, and leaves superior stability. Ideal for 

immobilization of antibodies, as well as peptides and nucleic acids. Coupling reactions can be carried out in an organic 

solvent. 


• Chemical linkage of ligand: Stable amide 

(carbamate) linkage 




(ligand dependent) 

• Coupling conditions: pH 8-9 

• Particle size range: 45-165µm 



• Temp.: 4 - 25°C 

• Time: 4-24h 


• Time: 2-16h 

• Temp: 4-25°C 


• Supplied as preswelled gel 

suspension in acetone. 

• Coupling conditions: 

See www.bio-world.com 

OH 

OH 

#506410 1g $ 57.20 

#506411 5g 

#506412 10g $ 249.00 

NH 2 

#506958 5ml $ 67.50 

#506960 15ml $ 129.00 

#506962 25ml $ 

o 

o 

CNBr 

NaOH 

R 

o 

C 

o 

C 

N 

NH 2 

NH 

N 

o 

OH 

o 

OH 

R 

R 

C N 

+ 

NH 2 

C NH 

$ 159.00 

R 

198.70 


EAH-Activated Sepharose 

Preactivated medium for immobilization of ligands containing free carboxy groups using a carbodiimide such as EDC 

(Cat. #507077). No blocking reaction is necessary following the coupling reaction. The long spacer arm, a total of 15 

atoms, is ideal for the presentation of small ligands such as peptides and organic acids. Supplied in 90% ethanol. 

#506950 1ml $ 46.70 

#506951 25ml 

$ 118.00 



F: 614.792.8685 


• Group to be coupled: COOH 

• Chemical linkage of ligand: Epoxy 

• Matrix: Agarose, 4% Agarose Beads 



• pH stability: 4.5-6.0 

• Temp.: 4 - 25°C 

• Time: 4-24h 


for protocols visit www.bio-WORLD.com 

#506452 50ml $ 198.70 

 

 

 

 

 

 

 

 

 

7

= 

= 

= 

= 


ECH-Activated Sepharose 

Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups. Ligands can be coupled in a 

single step procedure in the presence of a coupling reagent (not provided) and a water-soluble carbodiimide, such as N- 

ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The matrix provides a 10-atom spacer arm 

convenient for coupling of small ligands. No blocking reaction is necessary following the coupling reaction. Coupling 

reaction can be carried out with organic solvents. Supplied as preswelled gel suspension in buffered saline solution. 


• Chemical linkage of ligand: Stable 

amide linkage. 


• MW exclusion limit: Approximately 2x10 7 

Epoxy-Activated Sepharopore 

Preactivated high gel strength bioAgarose for immobilization of ligands containing amino (primary or secondary), 

hydroxyl or thiol groups. Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and 

carbohydrates. Provides a hydrophilic 12-atom spacer arm upon ligand coupling. Supplied as lyophilized powder. 1g of 

powder swells to approx. 3-4 ml gel. Similar to epoxy-activated Sepharose but lower cost. 

• Group to be coupled: -NH 2 , -OH or –SH 

• Chemical linkage of ligand: 

stable secondary amide linkage 

• Matrix: Agarose, 4% Beads Cross-linked 

• MW: exclusion limit: Approx 2x10 7 

NHS-Activated Sepharose 

• Particle Size (range): 45-165 µm 

• Coupling conditions: pH 4.5-6, 

• Temp: 4-25°C 

• Time: 1.5-24h 



• pH Stability: 3-10 


• Coupling conditions: pH 

9-10 (amino ligands), pH 11 

(hydroxyl ligands), pH 7.5- 

8.5 (thiol ligands) 

• Working Temp.: 4-75°C 

• Time: 16-48 hours 

• Organic solvents possible 


Preactivated matrix for immobilization of ligands containing a primary amino group. N-hydroxyl succinimide ester forms 

a stable amide (peptide) bond with the primary amine of ligand. The stability of amide bond at high pH (up to pH 12) 

allows for a very stringent wash procedure during affinity purification using NHS-activated ligand coupling. Neutral 

pH coupling conditions make it ideal for immobilization of antibodies, antigens and other proteins sensitive to pH extremes. 

The coupling reaction can also be carried out in an organic solvent. Provides a 10-atom spacer arm upon ligand 

coupling. Supplied as preswollen gel suspension in 100% isopropanol to protect the active groups. 

New 

#506954 15ml $ 46.50 

#506956 25ml $ 149.70 

#506957 50ml $ 249.00 

+ NH2 

O 

O C NH (C H 2 ) 5 C OH 

EDC NH 2 R 

+ NH2 

O 

O C NH (C H 2 ) 5 C NH R 

#506413 1g $ 58.00 

#506414 5g $ 198.00 

#506415 10g $ 347.00 

 

 

 

 

 

 

 

 

 

#506416 5ml $ 49.80 

#506417 15ml $ 86.00 

#506418 25ml $ 129.50 

#506419 50ml $ 198.00 

8 

• Groups to be coupled: primary –NH 2 

• Chemical linkage of ligand: Amide bond 


• MW exclusion limit: Approx. 2x10 7 

• Particle Size range: 45-165µm 

• Substitution: 16-23µmol 

NHS/ml medium 

• pH Stability: 3-12 


• Coupling conditions: pH 6-9 

• Temp.: 4-25°C 

• Time: 2-16 hours 

• Storage: 2-8°C


Ligand Coupled 

Arginine Sepharose 

This matrix is used to isolate or remove a number of different serine proteases, eg. Prekallikrein, Clostripain and 

Plasminogen activator from a wide range of materials. L-Arginine is coupled to Sepharose 4B, predominantly through 

its amino group, leaving the guanidine and carboxyl groups to react reversibly with the target protein. Desorption with 

ionic gradient or steps. Arginine Separose also has a wide pH and detergent compatibility. 

• Ligand: L-Arginine 

• Ligand coupling method: Epoxy 

• Ligand density: 20-40µmol 

Arginine/ml medium 

• Matrix: Agarose 4% Beads 

Benzamidine Sepharose 




Used in the removal and/or purification of serine proteases, e.g. Thrombin. p-Aminobenzamidine (pABA) immobilized 

on Sepharose 4B matrix gives high productivity and allows for easy scale-up. Effective in the removal of thrombin and 

factor Xa after tag cleavage of recombinant proteins. 

• Ligand: p-Aminobenzamidine (pABA) 

• Ligand coupling method: Amide 

• Ligand density: >12µmol p- 

Aminobenzamidine/ml medium 

• Binding capacity: >35mg Trypsin/ml 

• Matrix: Agarose 4% Beads 


• pH stability: 2-8°C 

• Storage: 2-12ºC 

New 

Save 

25% 

#506489 10ml $ 74.00 

#506490 25ml $ 149.50 

#506494 50ml $ 298.00 

#506491 10ml $ 87.00 

#506492 25ml $ 198.00 

#506493 50ml $ 317.00 


activeligands> liveproteins SM 



F: 614.792.8685 


9


Blue Sepharopore CL-6B 

Dye-ligand affinity matrix for purification of albumin, enzymes binding to NAD + and NADP + , coagulation 

factors, interferons and related proteins. The ligand, Cibacron Blue, is covalently coupled to Sepharopore 

through the chlorotriazine ring. Broad spectrum affinity that can be used as a general purpose protein 

purification tool and specifically used for removal of albumin from serum. Supplied as lyophilized matrix powder (1g 

swells to approx. 4ml) or as a suspension in 20% ethanol. Equal or superior to Blue Sepharose. 

• Ligand: Cibacron Blue F3G-A 

• Ligand coupling method: Triazine coupling 

• Ligand density: 8µ mol Cibacron Blue/ml 

• Binding capacity: ≥ 15 mg bovine serum 

albumin/ml drained gel 

DEAE Sepharopore 

• Matrix: Highly cross-linked 

agarose, 6%Beads 

• MW exclusion limit: 4 x 10 6 



• Coupling conditions: pH 9 - 10, 

Temp. 4 - 25°C, Time 2-16h 


DEAE bioMatrix is the most widely used chromatographic media for protein purification. BioWorld’s DEAE 

Sepharopore is prepared by covalent attachment of diethylaminoethyl to Sepharopore CL-6B. DEAE 

Sepharopore is a weak anion exchanger for binding and resolving of proteins of all pI values. Diethylaminoethyl 

remains charged over a wide working range of pH 3-9. An excellent matrix for analytical and preparative fractionation 

of proteins. Supplied as pre swollen in 20% ethanol. Bulk quantity available. 

• Ligand: diethylaminoethyl 

• Max flow rate: 150cm/h 

• Binding capacity: 20-70mg/ml 

• Matrix: Agarose 6% 

• Bead size range: 50-170 µm 

. 



#506480 5ml $ 34.50 

#506482 25ml $ 107.00 

#506484 50ml $ 185.60 

#506485 100ml $ 347.00 

#506929 50ml $ 80.00 

#506930 100ml $ 158.00 

#506931 500ml $ 340.00 

CH 2CH 3 

O CH 2CH 2 

N: 

CH 2CH 3 

10 

Gelatin Sepharopore 

Affinity matrix for the purification of fibronectin. Gelatin covalently coupled to Sepharopore 4B by CNBr activation. 

Supplied as suspension in 0.5M NaCl. 

• Ligand: Bovine gelatin 

• Ligand coupling method: CNBr activation 

• Ligand density: ≥ 5 mg gelatin/ml 



• Particle size: 50-170 µm 

• pH stability: 3–10 


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

New 

New 

#506908 5ml $ 29.70 

#506910 10ml $ 89.50 

#506912 25ml $ 98.25

Glutathione Sepharose 4B 

Reduced glutathione (GSH) covalently coupled to Sepharose. For affinity purification of proteins containing 

glutathione S-transferase (GST) fusion tag. Supplied as a suspension in 20% ethanol. One ml drained resin binds more 

than 5mg of recombinant GST protein. Most suitable bioAffinity matrix for recombinant protein purification. 

• Ligand coupled: Reduced glutathione (GSH) 

• Ligand coupling method: Epoxy activation 

• Matrix: Cross-linked agarose, 4% Beads 

• Binding capacity: ≥ 5 mg GST/ml 

drained gel 

Heparin Sepharose 

• Ligand: Porcine heparin 


• Ligand density: 5mg heparin/ml 

• Binding capacity: Approx. 2 mg bovine 

anti-thrombin/ml drained resin 

• MW exclusion limit: approx. 2 x 10 7 



Affinity matrix with immobilized heparin (from porcine intestinal mucosa) by CNBr activation coupling method. For 

affinity purification of coagulation factors such as antithrombin III, DNA binding proteins, restriction endonucleases, 

lipases, growth factors, lipoproteins and steroid receptors. Supplied as suspension in 0.5M NaCl. 







#506400 2x2ml $ 59.70 

#506405 15ml $ 89.70 

#506404 10ml $ 170.00 

#506406 50ml $ 396.00 

#506424 4x1ml $ 49.50 

#506425 10ml $ 89.70 

#506426 25ml $ 149.50 

#506428 50ml $ 298.70 




F: 614.792.8685 


Metal Chelating Sepharopore 

Affinity matrix for proteins with metal binding properties with exposed amino acids such as histidine and cysteine. 

Iminodiacetate immobilized on the bioAgarose matrix allows versatile chelation of divalent metal ions of choice (not 

included). Cu or Ni charged metal-chelating Sepharopore is ideal for purification of recombinant proteins containing 

(His) 6 

fusion tag. Supplied as suspension in 20% ethanol. 

• Chelating group: Iminodiacetic acid 

• Chelating group coupling method: Epoxy 

activation 

• Matrix: Cross-linked agarose, 4% Beads 


#506431 25ml $ 196.50 

• Particle size range: 

• Binding capacity: ≥ 15mg 

 

50-170 µm 

(His) 6 

-tagged protein 

CH 2 

C 

O 

• Metal ion capacity: 

• Spacer arm: 14-atom 

O CH 2 CH CH 2 O (C H 2 ) 3 O CH CH 2 N Mn 

 

O 

CH 

≥ 20 µmol Zn 2+ 

 

 

/ml medium ; • pH stability: 3-13 

2 C 

O 

µmol Co 2+ /ml medium • Storage: 2-8°C 

Mn = Cu 2+ , Zn 2+ , NI 2+ , Co 2= 

, etc... 


New 

#506428 5x1ml $ 78.50 

#506429 5x3ml $ 149.70 

#506430 10ml $ 97.80 

= 

= O 

11


Thiophilic Agarose 

Thiophilic Agarose is a versatile affinity matrix designed to couple sulfhydryl containing proteins or low molecular 

weight thiols. These proteins can be coupled under mild, nondenaturing or reducing conditions. This support 

provides an alternative to primary amine, carbonyl or other thiol coupling supports. When sulfhydryl groups are not 

present, they may be generated by reducing disulfide bonds that are present. Alternatively, sulfhydryl groups may 

be introduced to your protein with Traut’s reagent (2-Iminothiolane•HCl), catalog number 507141, which reacts with 

primary amines and leaves an exposed sulfhydryl group available for coupling. The crosslinked agarose support to 

which the TNB-Thiol is covalently coupled which withstands strong denaturants, low pH conditions and some solvent 

exposure. Careful consideration should be given to the stability of the proteins or macromolecules before using such 

extreme conditions with an affinity matrix. 

#506477 5ml $ 65.00 

#506478 10ml $ 145.00 

#506479 25ml $ 229.00 

CH 2CH 3 


12 

• Supplied: 50% slurry in 0.5 M Acetic Acid 

• Ligand: Approximately 5 µmoles of TNB per ml of settled gel 

• Matrix: Cross-linked Agarose, 6% Beads 

• Binding: Sulfhydryl containing proteins or low molecular weight thiols 

• Storage 2-8ºC 


Protein Ligand Coupled 

Avidin Sepharopore 

Used for binding biotinylated macromolecules. Affinity purified egg white avidin is dissociated into individual subunits 

and conjugated to Sepharopore Matrix, similar to Sepharose 4B. The matrix is suspended in 50% glycerol, 0.15M 

NaCl, 0.01M sodium phosphate (pH 6.8) containing sodium azide. Avidin-biotin binding is one of the strongest in biological 

systems. 

• Ligand: egg white avidin 

• Ligand Density: Approx. 2.4mg Avidin/ml drained resin 


• Spacer Arm: 12 atoms 

• Binding capacity: 20-40 nmol of biotin/ml of gel 


New 

O CH 2CH 2 

N: 

#506469 5ml $ 78.00 

#506470 10ml $ 137.00 

 

 

 

 


CH 2CH 3

Calmodulin Sepharose 

Affinity matrix with immobilized bovine Calmodulin. For affinity purification of calmodulin binding proteins, including 

calmodulin regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding 

peptide fusion tag. Supplied as suspension in 20% ethanol. 

• Ligand: Calmodulin Protein 

• Ligand Coupling Method: CNBr Activation 

• Ligand Density: Approx. 1.5mg Calmodulin/ml drained resin 

Con-A Sepharose 

• Ligand: Con A protein 


• Ligand density: > 8-10mg/ml 

• Binding capacity: > 25 mg HRP/ml drained gel 

E. coli Protein Sepharopore 


• MW: exclusion Limit: Approx. 2x107 



Affinity matrix for purification of macromolecules containing D-mannosyl or D-glucosyl groups such as glycoproteins, 

glycolipids and polysaccharides. The ligand, Concanavalin A (Con A), is covalently coupled to bioAgarose Sepharose 

via CNBr activation. Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes. Supplied as 

suspension in 20% ethanol. 

• Matrix: agarose, 4% Beads 

• Exclusion limit: approximately 2 x 10 7 



E. coli proteins were extracted from three strains of E. coli and coupled to CNBr-activated Sepharopore 4B. Antibodies 

against E. coli proteins (from a contaminant in an antigen) can be removed by solid phase immunoaffinity chromatography 

over the E. coli Protein Sepharopore column. Supplied as a gel in PBS and sodium azide. 

New 

#506460 1 ml $ 87.00 

#506461 5 ml $ 149.50 

#506462 10 ml $ 247.00 

 

+ 

 

#506464 2 x 1ml $ 29.70 

#506466 5 ml $ 49.60 

#506465 25 ml $ 147.00 

#506468 50 ml $ 244.00 

#506467 1 ml $ 58.00 

#506471 5 ml $ 174.50 

#506472 10 ml $ 248.00 


• Ligand coupling method: CNBr 

• Ligand Density: Approx. 2.4mg E. coli proteins/ml 

drained resin 



Clean your antibodies 

raised against EColiexpressed 

proteins 

New 


13 

for protocols visit www.bio-WORLD.com


IgG Sepharopore 

An affinity chromatography matrix consisting of rabbit IgG bound to cross-linked 6% agarose. This matrix is coupled 

through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. IgG 

Sepharopore may also be used to immunoprecipitate Protein-A produced by some prokaryotic organisms. 

• Ligand: IgG 

• Ligand coupling method: epoxy activation 

• Binding Capacity: Minimum 2mg (Protein A)/ml drained gel at pH 7.5 



• pH stability: 3-9 (use), 2-10 (cleaning) 


#506966 1ml $ 54.00 

#506967 5ml $ 124.00 

#506968 10ml $ 197.00 


KLH Sepharopore 

Affinity Matrix with immobilized KLH (Keyhole Limpet Hemocyanin). For affinity purification of antibodies generated 

against KLH conjugated peptide antigens. Supplied as suspension in 0.1x PBS and sodium azide. Ideal for removing KLH 

antibodies from polyclonal serum to obtain high specificity and clean western blots. 

• Ligand: Keyhole limpet Hemocyanin (KLH) 

• Ligand Coupling Method: CNBr activation 

• Ligand Density: Approx. 3 mg KLH/ml drained resin 


bioLAM (Lectin Affinity Matrix) 

• MW exclusion Limit: 2x10 7 




The lentil (Len culinaris) lectin (agglutinin) protein (LCA) recognizes sequences containing linked mannose residues. Like 

other mannose-specific lectins, divalent cations like calcium and manganese are required for sugar binding activity. LCA 

has been employed to separate lymphocyte populations and to purify numerous glycoproteins. 

• Equal to Lectin Sepharose 





New 

#506495 2x1 ml $ 59.70 

#506496 10ml $ 168.00 

#506497 25ml $ 298.00 

#506473 5 ml $ 98.00 

#506474 10 ml $ 165.00 

#506481 25ml $ 298.00 

See also MagneTalon 

for various LAMs. 

(Page 22) 

New 

14

Protein-A Sepharose 

Affinity matrix with immobilized Protein-A (from Staphylococcus aureus) by CNBr activation coupling method. For 

purification and/or fractionation of IgG. Reliable tool to purify monoclonal and polyclonal IgG from ascites, 

serum and cell culture supernatants. Supplied as suspension in 0.5M NaCl or as lyophilized powder. 

• Ligand: Recombinant protein A from 

Staphylococcus aureus 


• Binding capacity: Approx. 20mg human 

IgG/ml drained gel 

Protein A-Red Sepharopore 


• MW: exclusion limit: Approx. 2 x 10 7 


• pH stability: 3–9 (for use), 

2–10 (for cleaning) 


* Supplied as ready to 

use columns. 

Highly visible, red colored Protein A affinity gel matrix designed for use in immunoprecipitation experiments. It 

contains Protein A, covalently attached to Sepharopore 4B beads. Functions to bind the antibody (IgG) in the same 

manner as non-colored Protein A Sepharose. The red color gives the agarose affinity gel enhanced visibility in 

order to aid in manipulations such as repetitive washing & recovery of antigen. This leads to greater experimental 

reproducibility & more accurate quantitation of the immunoprecipitated proteins. 

• Ligand: Recombinant protein A from 

Staphylococcus aureus 





• MW: exclusion limit: Approx. 2 x 10 7 





New 

#506442 2x.8ml* $ 69.50 

#506443 2ml $ 89.70 

#506444 5ml $ 249.50 

#506475 1ml $ 79.70 

#506476 5ml $ 252.00 


Protein G Sepharose 

Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method for purification 

and/or fractionation of IgG subclasses, which bind poorly to protein A. Reliable tool to purify monoclonal and polyclonal 

IgG from ascites, serum and cell culture supernatants. Supplied as suspension in 0.5M NaCl. 

#506450 1ml $ 127.00 

#506452 5ml $ 390.00 

#506454 10ml $ 597.00 

• Ligand: Native protein G from 

Streptococcus sp. 




• Matrix: Agarose, 4% Beads • Storage: 2-8°C 

• MW: exclusion limit: Approx. 

2 x 10 7 





15

Streptavidin- Sepharose 


Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. For binding biotinylated 

compounds. Supplied as buffered saline solution 

• Ligand: Native streptavidin (from 

Streptomyces avidinii) 


• Ligand density: 2 mg/ml 

• Binding capacity: ≥ 20 µg biotin/ml 

drained gel 





• Elution buffer: 50 mM 

ammonium acetate, 0.5 M 

NaCl, pH 4.0 (provided) 


#506914 1ml $ 54.00 

#506916 10ml $ 379.00 

Wheat Germ Lectin Sepharopore-MB 


Wheat Germ Lectin (agglutinin) binds specifically to cells, subcellular particles, glycoconjugates and polysaccharides containing 

N-acetylglucosamine residues. In WGA- 6MB, the lectin is covalently coupled to Sepharopore 6MB. This affinity 

adsorbent is useful for the general fractionation of glycoconjugates, but the large bead size also facilitates the specific 

binding of cells with practically no physical entrapment of the cells. WGA Sepharopore 6MB is supplied in 0.9% NaCl 

containing 0.04% sodium azide as a preservative. 

• Binding Specificity: Depends on the composition 

and structure of the glycoconjugate or 

polysaccharide 


• Ligand Concentration: 5mg Wheat germ lectin/ 

ml drained gel 

• Matrix: Agarose, 6% Macrobeads 

• Exclusion limit (Mr): 40 megadaltons 

• Bead size range: 250µm-400µm diameter 

• Ligand source: Triticum vulgaris 

• pH stability: 4–9 

• Storage: 4-7°C (Do not freeze) 

#506963 1ml $ 88.00 

#506964 5ml $ 187.00 

#506965 10ml $ 276.00 

#506961 25ml $ 398.00 

CH 2CH 3 

WGA 

O CH 2CH 2 

N: 

CH 2CH 3 

New 

Ubiquitin-Sepharose 

Covalently linked ubiquitin to Sepharose beads via primary amine coupling leaving the C-terminus free. Useful for 

binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3’s) and ubiquitinterminal 

hydrolyases. (ucms) 

#506451 2x1ml $ 96.00 

#506446 10ml $ 195.00 

• Ligand: Red blood cell ubiquitin 

• Ligand coupling method: CNBr 


• Binding capacity: 2mg/ml resin 

• Storage 4ºC 

• Supplied: in 20% ethanol solution. 

Study 

ubiquitinylation 

and proteasome 

pathways! 

New 

16


Sugar Ligand Coupled 

Mannose Sepharopore 

Mannose Sepharopore 4B is prepared by covalent linkage 

of D- Mannose to Sepharopore 4B via the hydroxyl groups. 

The likely site for coupling is at the C-6 position. Mannose 

Sepharopore may be used to isolate mannose-binding 

proteins such as Concanavalin A (Cat #714279 and 714271). 

This resin can be used for lectin purification. Please consult 

literature for suggested buffers and optimal pH for specific 

proteins . Mannose Sepharopore 4B is supplied as a 

suspension in 20% ethanol. 

• Ligand: D-Mannose 

• Ligand coupling method: covalent linkage 

• Binding capacity: 2 mg/ml 


• Spacer Arm: 5 atoms 



CM - Sepharopore 

CM Sepharopore CL-6B is a weak cation exchanger 

bioAgarose matrix with excellent flow properties and high 

capacity for proteins of all pI values. The ion exchange group is 

a carboxymethyl group which remains charged and maintains 

consistently high capacity of bindiing over the entire working 

range, pH 4-7. 

H 

HO 

OH OH 

H 

H 

H 

O 

O 

 

HO 

OH 

H 

O 

 

H 

New 

#506386 1ml $ 54.00 

#506387 5ml $ 148.00 

#506388 10ml $ 227.00 

#506392 25ml $ 398.00 

H 

H 

OH 

O 

H 

1 

H 

 

O 

HO 

 

H 

H 

OH 

H 

H 

OH O 

H 

 

 

#506389 5ml $ 74.00 

#506390 25ml $ 209.00 

#506391 50ml $ 347.00 

O 

 

HO 

H 

O 

H 

 

H 

OH 

O 

H 

 

 

H 

O 

Adjustable Endpiece 

Glass 

Frit 

‘O’-ring 


• Capacity: Bovine IgG (MW 160 000), 9.5 mg/ml 

• Ribonuclease (MW 13 700), 12 mg/ml 

• Max. linear flow rate: 150 cm/h at 25 °C 

• pH working range: 4-7 

Fixed Endpiece 




• Bulk quanity available. 

 

 

 

 

 

© 

New 

for all your column requirements 

(from 3mm x 10mm wide-10cm x 100cm 

long) contact: 1-888-bioplus 

17




Nucleotide Ligand Coupled 

AMP Sepharopore 

5-AMP Sepharopore 4B interacts strongly with NAD + dependent dehydrogenases and ATP-dependent enzymes. 

Selective elution with gradients of NAD + or NADP + has allowed the resolution of complex mixtures of dehydrogenases 

isoenzymes using 5’ AMP Sepharopore 4B. 5-AMP Sepharopore 4B is synthesized in several steps. Diaminohexane is 

linked to AMP via the N6 position of the purine ring. The derivatized AMP is then immobilized to Sepharopore 4B via 

aminohexane spacer. Equal to AMP Sepharose 

• Matrix: Cross-linked Agarose 4% Beads 


• Ligand: AMP 

• Ligand density: 2 µmol AMP/ml drained medium 

• Binding capacity: >10 mg lactate dehydrogenase/ml 

drained medium. 

NAD Sepharopore 


• Max Flow Rate: 75cm/h at 25°C 


NAD covalently bound to Sepharopore has a high affinity for NAD-dependent enzymes and dehydrogenases. NADcoupled 

matrices have has been used to purify Vibrio cholerae toxin, diptheria toxin, and other NAD binding proteins. 

Equal to NAD Sepharose 



• Ligand: NAD 

• Ligand density: 2 µmol AMP/ml drained medium 

• Binding capacity: >10 mg lactate dehydrogenase/ml 





New 

#506487 1ml $ 69.70 

#506488 5ml $ 227.60 

#506380 1ml $ 78.00 

#506381 5ml $ 237.60 

#506382 10ml $ 398.00 

NADP Sepharopore- (Sepharose) 

New 

NADP covalently bound to Sepharopore has a high affinity for NADP dependent enzymes and dehydrogenases. 

Acetyl-CoA binds to NADP-Sepharopore 12 times higher than NAD-Sepharopore. Has also been used to bind extracellular 

nucleases. 

#506383 1ml $ 82.00 

#506384 5ml $ 248.60 

18 



• Ligand: NADP 

• Ligand density: 2 µmol NADP/ml drained medium 

• Binding capacity: >protein/ml 





New 

#506385 10ml $ 417.00


BlackPearl Magnetic Beads 

BlackPearl -CAM (Calmodulin) 

Affinity matrix with Calmodulin covalently coupled to 

BlackPearl magnetic beads. For affinity purification 

of calmodulin-binding proteins including calmodulinregulated 

proteins in eukaryotic cells and recombinant 

proteins containing calmodulin-binding peptide fusion 

tag. Supplied as a suspension in 0.5 M NaCl. 

• Matrix: Cross-linked Agarose 6% with magnetite 




BlackPearl-L (Wheat Germ Lectin) 

Wheat germ lectin (from Triticum vulgaris) conjugated 

to BlackPearl Magnetic Beads. Wheat germ agglutinin 

is a 36,000 molecular weight protein consisting 

of two identical subunits. The receptor sugar for WGA 

is N-acetylglucosamine, with preferential binding to 

dimers and trimers of this sugar. WGA can bind oligosaccharides 

containing terminal N-acetylglucosamine 

or chitobiose, structures which are common to many 

serum and membrane glycoproteins. Bacterial cell wall 

peptidoglycans, chitin, cartilage glycosaminoglycans 

and glycolipids can also bind WGA. 

• Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine, 

Chitin Hydrolysate, or 100 mM acetic acid 

• Ligand: 2 mg lectin/ml gel 

BlackPearl-S (Streptavidin) 

Streptavidin has a high affinity for biological compounds. 

bioWorld Black Pearl Magnetic Beads make 

resolution and recovery of biotin and biotinylated 

compounds easy and effective. Supplied in a buffered 

saline solution. 

#506921 1ml $ 88.00 

#506922 5ml $ 198.00 

#506932 10ml $ 347.00 

New 

#506923 1ml $ 84.00 

#506927 5ml $ 298.00 

#506933 10ml $ 397.00 

New 

#506914 1ml $ 54.00 

#506915 5ml $ 199.50 

#506916 10ml $ 347.00 

BlackPearl-A (Protein-A) 

An affinity matrix ideal for isolation and purification 

of immunoglobins on a small scale. Black 

Pearl-A magnetic beads consist of recombinant 

Protein A covalently coupled to BlackPearl. 

Protein A has a high affinity for subclasses of 

IgG from a variety of species, including human, 

rabbit, mouse and sheep. The matrix can be 

regenerated up to 6 times with minimal loss of 

binding capacity. It can be used to immunoprecipitate 

target proteins from crude cell lysates 

using a selected primary antibody. Specific antibodies 

can be covalently cross-linked to the Black 

Pearl-A surface to create a reusable immunoprecipitation 

bead, thus avoiding the coelution 

of antibody with the target antigen. 

• Matrix: Agarose 6%, cross-linked 




BlackPearl-G (Protein-G) 

BioWorld BlackPearl-G Magnetic beads are 

prepared by covalent immobilization of Protein- 

G (from Streptococcus sp.) by CNBr activation 

coupling. Protein G shows strong binding affinity 

to the Fc region of several classes of mammalian 

immunoglobulins. BlackPearl-G has 

been used as a reliable tool to purify monoclonal 

and polyclonal IgGs from ascites, serum and 

cell culture supernatants. It is an ideal choice for 

affinity purification and/or fractionation of IgG 

subclasses which bind poorly to Protein-A such 

as bovine, goat, rat, and sheep IgG. Supplied as 

suspension in 100 mM Tris·Cl, pH 8.0, 500 mM 

NaCl, 0.05 % NaN 3 and 0.02 % thimerosal. 

#506917 1ml $ 78.00 

#506918 5ml $ 265.00 

#506934 10ml $ 375.00 

New 

#506919 1ml $ 98.00 

#506920 5ml $ 368.00 


• Ligand: Streptavidin (Streptomyces avidinii) 

• Ligand Coupling Method: CNBr-activation 

• Ligand: approx. 300nmol Biotin per ml gel 

• Matrix: Cross-linked Agarose 6%Beads with magnetite 


• Ligand: protein-G from Streptococcus sp. 

• Binding capacity: Approximately 20 mg human IgG/ml drained gel 

• Matrix: Cross-linked Agarose, 6% cross-linked 


New 

New 

19



20 


Silica Coated Magnetic Beads 

MagneTalon - H 

• Uniform superparamagnetic 

beads with a hydrazide surface 

group. 

• It is widely used to covalently couple aldehyde- or ketone-containing 

ligands such as antibodies, lectins, glycoproteins or carbohydrates 

through the formation of stable hydrazone linkage. 

• This is a powerful method to immbolize proteins and keep critical 

active sites free. 

• Size: 1 µm 

• Quantity: 30 mg/ml 

Fe 

MagneTalon - IDA 


beads modified with 

iminodiacetic acid. 

#506212 3ml $135.00 

#506213 10ml $ 395.00 

 

 

 

 

 

 

 

 

 

 

 

 

• They can also be further modified to carry different functional groups 

for other applications. 



Fe 

New 

#506216 3ml $115.00 

#506217 10ml $ 250.00 

 

 

 

 

 

 

 

 

 

 

 

New 

 

 

See page 22 

for PickPen 

> 

accelerate: highthroughputscreening 

MagneTalon - COOH 


beads with surface carboxyl 

group. 

#506200 

#506201 

5ml 

10ml 

$ 125.00 

$ 175.00 

• Proteins and nucleic acids can be covalently bound to these beads with 

the retention of biological activity. 

• Size: 1µm 

• Quantity: 20mg/ml 

• Quantity: 30mg/ml 

MagneTalon - CHO 


New 

Fe 

Epoxy-Activated MagneTalon 

Fe 

 

 

 

 

 

 

• Uniform superparamagnetic beads with an epoxy surface group. 

#506204 #506205 3ml 10ml $ 145.00 

$ 437.00 

• Widely used to covalently couple amine-, hydroxyl- and thiolcontaining 

ligands. 

• Such coupling methods can provide an extremely stable linkage 

between the ligand and the matrix. 


New 


beads with an aldehyde surface 

group. 

#506208 

#506209 

3ml 

10ml 

$145.00 

$ 437.00 

• Widely used to covalently couple ligands containing primary and 

secondary amino groups. 

• This coupling method provides an extremely stable linkage between 

the ligand and the matrix. 


New 

Fe


MagneTalon-P Polystyrene Coated Magnetic Beads 

Uniform polystyrene spheres containing magnemite(Fe 2 O 3 ) and magnetite(Fe 3 O 4 ). MagneTalon-P beads have a uniform distribution of Fe. An outer 

coating of polystyrene keeps all magnetic material encased and isolated from your sample. MagneTalon-P beads can be activated and covalently 

coupled with target-specific ligands. MagneTalon-P beads provide many advantages over conventional chromatographic techniques. Cell isolation 

with MagneTalon provides yields and purity greater than 90%. Produces biologically active, and pure samples. MagneTalon beads require minimal 

equipment: Sample of choice, test tubes, MPC magnet, and washing buffer. No columns needed! Isolation can be performed in 10-15 minutes. 

MagneTalon-P beads can be used with PickPen (see page 12). 

 

 

 

 

 

© 


MagneTalon-P (C-18) 

C18 - Polystyrene bonded phase with strong 

hydrophobicity; used for analytes of weak 

hydrophobicity from aqueous solutions. 

Typical applications include drugs and their 

metabolites in serum, plasma or urine, 

desalting of peptides, trace organics in 

environmental water samples and organic 

acids in beverages. Similar in behavior to 

reversed-phase HPLC columns. 

New 

#506500 1ml $ 70.00 

#506501 5ml $ 200.00 

MagneTalon-P-CX (Cation Exchanger) 

Magnetic separation of proteins via 

exploitation of differences in localized 

charged groups of proteins. Bead-bound 

positively charged groups reversibly adsorb 

charged molecules. Fractionation by adjusting 

pH and salt concentration during adsorption 

or elution to purify glycoproteins. 

#506512 1ml $ 60.00 

#506513 5ml $ 190.00 

New 


MagneTalon-P-AX (Anion Exchange) 

MagneTalon-P-CAM (Calmodulin) 


exploitation of differences in localized 

charged groups of proteins. Bead-bound 

negatively charged groups reversibly adsorb 

charged protein or nucleic acid molecules. 

Fractionation by adjusting pH and salt 

concentration during absorbtion. Elute by 

increasing ion concentration. 

BlackPearl S (Streptavidin) 

New 

#506503 1ml $ 60.00 

#506504 5ml $ 190.00 

Calmodulin covalently bound to MagneTalon 

polystyrene beads. For purification of 

calmodulin-binding proteins and calmodulinregulated 

proteins. Proteins containing a 

calmodulin binding peptide fusion tag can also 

be purified using MagneTalon-P -CAM. 

> 

accelerate: signaltransductionresearch 

#506515 1ml $ 70.00 

#506516 5ml $ 200.00 

New 

Find Calmodulin 

Binding Proteins 

21 


PickPen & QuickPick Kit 


MagneTalon Polystyrene Coated Magnetic Beads (contd.) 

MagneTalon-LCA 

The lentil Len culinaris lectin (agglutinin 

protein) (LCA) recognizes sequences 

containing mannose residues. Like other 

mannose-specific lectins, divalent cations 

calcium and manganese are required for sugar 

binding activity. LCA has been employed to 

separate lymphocyte populations and to purify 

numerous glycoproteins. 

MagneTalon-Fe (IMAC) 


exploitation of differences in net charge of 

proteins. Bead-bound negatively charged 

groups reversibly adsorb charged molecules. 

Fractionation by adjusting pH and salt 

concentration during adsorption or elution. 

#506506 1ml $ 70.00 

#506507 5ml $ 200.00 

New 

#506509 1ml $ 65.00 

# 506510 5ml $ 195.00 

New 

• Rapid purification and fractionation 

of RNA & proteins. 

• Use with optimized QuickPick purification 

kits and ready-to-use DNase/RNase free tips. 

FAST: • Move particles, not liquids to reduce processing steps 

EFFICIENT: 

• Avoid repeated aspirating and dispensing 

• Work with your choice of vessel format 

EASY: • Hand-held, carry PickPen: with you 

GENTLE: 

• Simple to learn, operate and maintain 

• Ensure high sample quality through rapid 

handling 

PickPen 

QuickPick Plasmid Kit 

• Avoid centrifugations and spin columns 

MagneTalon-A (Protein A) 

Protein-A covalently linked to MagneTalon 

polystyrene beads. For the magnetic 

separation and isolation of IgG subclasses from 

ascites, serum or cell culture supernatants. 

#051909 1 Pen $ 398.50 

#051920 1 Kit $ 145.00 

New 

#506521 1ml $ 70.00 

#506522 5ml $ 200.00 

New 

For other QuickPick Kits visit 

www.bio-world.com 

One of the most 

efficient tools for 

magnetic separation! 

MagneTalon-G (Protein G) 

StrepTalon-S (Streptavidin) 

Protein G covalently linked to MagneTalon 

polystyrene beads. Protein G binds Fc region 

of mammalian immunoglobulin. Ideal for 

purification and/or fractionation of IgG 

subclasses that poorly bind Protein-A. 

#506524 1ml $ 70.00 

#506525 5ml $ 200.00 

Streptavidin covalently linked to 

MagneTalon polystyrene beads. Ideal for 

the magnetic isolation and separation of 

biotinylated ligands for affinity separations. 

#506527 1ml $ 65.00 

#506528 5ml $ 195.00 

New 

New 

22 

All MagneTalon matrices are suitable for 

high throughput screenings



ZeroBind 8-Well Strips 

• Suggested Uses: Proteins which 

must not react with the surface 

• 8-Well Strip feature raised rim 

with flat bottom. 

Activated Microtiter Plates 

HighBind 8-Well Strips 

• Binding Capacity: 400ng 

IgG/cm 2 

• Binding Interaction: 

Hydrophobic & Ionic (-) 

• Protein Properties: Medium to 

High molecular weight 

Ligand-Coupled Microtiter Plates 

Carboxylated 8-Well Strips 


Covalent via crosslinkers 

• Binding Properties: Molecules 

with an amino group on 

their structure 

#509846 up to 20 $ 2.10 ea 

#509847 21-100 $ 1.98 ea 

#509848 101-250 $ 1.85 ea 

• Suggested Uses: Assays where the adsorbed molecule must work in 

excess (up to 400ng/cm 2 ), ELISA determination for IgG (indirect ELISA 

test) or ELISA capture test for IgM detection. Suitable to set up 

competitive tests (molecule



Protein Ligand Coupled Microtiter Plates 

Calmodulin (Ca 2 + Binding Protein) 


Hydrophobic 

#509873 up to 20 $ 24.50 ea 

• Suggested uses: interactions #509874 21-100 $ 19.70 ea 

with proteins involved in 

glycogen metabolism, 

#509875 101-250 $ 15-40 ea 

interactions with factors involved in neurotransmission, and 

interactions with enzymes involved in the NAD + /NADP + Phosphorylation 

New 

Concanavalin-A Coated 

• Binding Interaction:High 

Affinity for terminal 

D-Mannosyl & D-Glucosyl 

residues 

• Suggested uses: Used to 

#509858 

#509859 

#509860 

up to 20 

21-100 

101-250 

$ 6.30 ea 

$ 4.90 ea 

$ 4.20 ea 

immobilize glycoproteins or carbohydrates containing these groups. 

Jacalin Coated 

• Binding Interaction: High 

affinity for D-Galactose 

#509861 up to 20 $ 7.95 ea 

residues of proteins such as #509862 21-100 $ 6.45 ea 

IgA1 & cell membranes 

• Suggested uses: Human IgA1 

#509863 101-250 $ 5.75 ea 

specific binding, separation of immunocomplexes antigen-antibody 

Protein-A Coated 

New 

• Binding Interaction: Specific 

for Fc region of IgG 

• Suggested uses: Binds the Fc 

region of Immunoglobulin 

(IgG) molecules w/o interacting 

#509852 

#509853 

#509854 

up to 20 

21-100 

101-250 

$ 8.35 ea 

$ 7.05 ea 

$ 5.55 ea 

at the antigen binding site; Including species - human, mouse, horse, 

dog, rat, cat, sheep, rabbit and pig 

24 

Fucose binding Lectin Coated 

• Ulex europaeus Agglutin I 


#509858 up to 20 $17.60 ea 

glycoproteins & glycolipids #509859 21-100 $15.20 ea 

containing linked 

fucose residues 

#509860 101-250 $14.00 ea 

• Suggested uses: Binding of ABO blood group glycoconjugates; 

preferentially binds blood group O & has been used to determine 

secretor status. An excellent marker for human endothelial cells 

Heparin Coated 

New 

• Binding Interaction: Ionic 

• Suggested uses: binding of 

coagulation factors such as 

antithrombin III, DNA binding 

proteins, restriction 

#509894 

#509895 

#509896 

up to 20 

21-100 

101-250 

$ 6.50 ea 

$ 5.20 ea 

$ 4.70 ea 

endonucleases, lipases and growth factors. 

Poly-L-Arginine Coated 


density of-Amino/-Carboxyl/ 

guanidino groups able to react 

through electrostatic & 

stereospecific bonds 

#509870 

#509871 

#509872 

up to 20 

21-100 

101-250 

$ 9.80 ea 

$ 7.50 ea 

$ 6.25 ea 

• Suggested uses: Interactions w/serine proteases & maturation 

promoting factors 

Poly-L-Lysine Coated 

#509867 up to 20 $ 7.00 ea 


density of-Amino/-Carboxyl 

groups able to react 

through electrostatic & 

stereospecific bonds 

#509868 

#509869 

21-100 

101-250 

$ 5.40 ea 

$ 4.50 ea 

• Suggested uses: Interactions w/ plasminogen & plasminogen activator. 

coated microtiter plates for high throughput screening 

New

Biotin (Vitamin H) Coated 


Streptavidin/Biotin 

noncovalent ionic bound 

• Suggested uses: interactions 

with Avidin & Streptavidin 

Protein-G Coated 

• Binding Interaction: Specific for 

Fc of IgG 

• Protein Properties: IgG 

• Suggested uses: Binds the Fc 

region of Immunoglobulin 

#509876 up to 20 $ 9.80 ea 

#509877 21-100 $ 8.50 ea 

#509855 up to 20 $ 10.85 ea 

#509856 21-100 $ 9.05 ea 

#509857 101-250 $ 7.75 ea 

(IgG) molecules without interacting at the antigen binding site. 

SBA (Soybean agglutinin) Coated 


preferentially binds to 

oligosaccharide structures with 

Terminal or ß-linked 

N-Acetyl galactosamine and, to a 

lesser extent, galactose residues 

#509891 

#509892 

#509893 

up to 20 

21-100 

101-250 

$ 7.60 ea 

$ 6.00 ea 

$ 5.30 ea 

• Suggested uses: Used in glycoprotein binding, cell surface proteins, 

agglutination assays. 

Streptavidin Coated 

• Binding Capacity: 19pmol/biotin 


Streptavidin/Biotin, strongest 

non covalent biological interaction 

• Binding Properties: Any biotinylated 

protein / nucleic acid 

#509849 

#509850 

#509851 

up to 20 

21-100 

101-250 

$ 9.80 ea 

$ 8.25 ea 

$ 7.35 ea 

• Suggested uses: Proteins, Peptides, Polysaccharides, Oligonucleotides, 

DNA fragments. 

Wheat Germ Lectin (Triticum vulgaris) Coated 

• Binding Interaction: Specific 

binding of N-Acetyl-D- 

Glucosamine residue of the 

biochemical structure of 

glycoproteins, enzymes & 

cell membranes 

#509864 

#509865 

#509866 

up to 20 

21-100 

101-250 

$ 5.75 ea 

$ 4.85 ea 

$ 4.05 ea 

• Suggested uses: glycoprotein purification including membrane 

glycoproteins 



> 

accelerate: ELISAanalysis> 

highthroughputscreening 

25



Lectin Affinity 

Arachis hypogaea 

• Isolated from peanuts 

• Primary sugar specificity: 

Galactose 

• Preferential binding activity: 

Galactosyl (β-1,3) 

N-Acetylgalactosamine 

* Columns supplied as 

Sepharopore 4B-Lectin . 

#509955 25mg $ 145.00 

#510111 3ml $ 145.00 

Pure- #509954 5mg $ 40.00 

Column- #510110 0.8ml x 2 $ 96.00 

FITC- #510112 5mg $ 82.00 

#510113 25mg $ 132.00 

Datura stramonium 

• Isolated from jimson weed 

(thorn apple) seeds 

• Primary sugar specificity: N- 

Acetyl Glucosamine 


(β-1,4) N-Acetylglucosamine 

Pure- #509967 1mg $ 35.00 

#509968 5mg $ 80.00 

FITC- #510137 1mg $ 68.00 

#510138 5mg $ 127.00 

Rhodamine- #510139 1mg $ 68.00 

#510140 5mg $ 128.00 

Texas Red - #510141 1mg $ 69.00 

#510142 5mg $ 130.00 


Concanavalin A 

• Isolated from jackbean seeds 

• Primary sugar 

specificity: Glucose/Mannose 


α-linked Mannose 



#510115 25mg $ 138.00 

Texas Red - #510116 5mg $ 92.00 

#510117 25mg $ 145.00 

#714279 1g $ 96.50 

#510130 3ml $ 145.00 

#510132 10mg $ 118.00 

Rhodamine- #510114 5mg $ 82.00 

Pure- #714271 250mg $ 29.70 

Column- #510129 0.8ml x 2 $ 127.00 

FITC- #510131 5mg $ 67.00 

Rhodamine- #510133 5mg $ 68.00 

Dolichos biflorus 

• Isolated from horse gram seeds 


N-Acetyl Galactosamine 


α-linked N-Acetylgalactosamine 

Glycine max 

• Isolated from soybean seeds 


Galactose/N- 

Pure- #509961 5mg $ 60.00 

#509962 10mg $ 95.00 

FITC- #510143 5mg $ 85.00 

#510144 10mg $ 128.00 

Rhodamine- #510145 5mg $ 95.00 

#510146 10mg $ 167.00 

Texas Red - #510147 5mg $ 86.00 

#510148 10mg $ 158.00 

Pure- #509956 10mg $ 80.00 

#509957 25mg $ 175.00 

#510134 10mg $ 120.00 

Texas Red - #510135 5mg $ 68.00 

#510136 10mg $ 122.00 

Enquire for bulk Con A 

call 1•888•bioPLUS 

Acetylgalactosamine 


Terminal or β-linked 

N-Acetylglucosamine 



Column- #510158 0.8ml x 2 $ 125.00 

#510159 3ml $ 145.00 

FITC- #510160 10mg $ 74.00 

#510161 25mg $ 138.00 

Rhodamine- #510162 10mg $ 75.00 

#510163 25mg $ 148.00 

26 

Texas Red - #510164 10mg $ 76.00 

#510165 25mg $ 149.00 


Griffonia simplicifolia (Call for pricing) 

• Isolated from Griffonia Simplicifolia seeds 

• Primary sugar specificity: Galactose/N-Acetyl Galactosamine 

• Preferential binding activity: A rich: N-Acetylgalactosamine, B rich: Galactose 

Lens culinaris 

• Isolated from lentil seeds 


Pure- #509942 10mg $ 75.00 

Glucose/Mannose 

#509943 25mg $ 120.00 

• Preferential binding activity: Column- #510169 0.8ml x 2 $ 87.00 

α-linked Mannose (divalent 

cations needed 

#510170 3ml $ 96.00 

FITC- #510171 10mg $ 62.00 

#510172 25mg $ 95.00 

Rhodamine- #510173 10mg $ 86.00 

#510174 25mg $ 145.00 

Texas Red - #510175 10mg $ 92.00 

#510176 25mg $ 148.00 

Psophocarpus tetragonolobus 

• Isolated from winged 

bean seeds 

Pure- #509964 1mg $ 35.00 


#509965 5mg $ 80.00 

N-Acetyl Galactosamine 

FITC- #510198 1mg $ 98.00 


α-linked Galactosamine 

#510199 5mg $ 138.00 

Rhodamine- #510200 1mg $ 98.00 

#510201 5mg $ 138.00 

Texas Red - #510202 1mg $ 98.00 

Triticum vulgaris (Wheat Germ) 

• Isolated from wheat germ 


Pure- #509973 10mg $ 65.00 

N-Acetyl Glucosamine 

#509974 25mg $ 110.00 


FITC- #510213 10mg $ 95.00 

N-Acetyl Glucosamine 

(dimers & trimers) 

#510214 25mg $ 159.00 

Rhodamine- #510215 10mg $ 95.00 

#510216 25mg $ 159.00 

Texas Red - #510217 10mg $ 95.00 

#510218 25mg $ 159.00 

Ulex europaeus 

• Isolated from furze gorse seeds 


Fucose 


α-linked fucose residues 

Pure- #509958 

#509959 

Column- #510219 

#510220 

2mg 

5mg 

0.8ml x 2 

3ml 

$ 55.00 

$ 90.00 

$ 96.00 

$ 127.00 

FITC- #510221 2mg $ 196.00 

#510222 5mg $ 298.00 

Rhodamine- #510223 2mg $ 196.00 

#510224 5mg $ 298.00 

Texas Red - #510225 2mg $ 196.00 

#510226 5mg $ 298.00 



Sambucus nigra 

• Isolated from elderberry bark 


Sialic Acid 


Sialic Acid attached to 

terminal Glucose in (α-2,6) 

#510203 5mg $ 138.00 

Pure- #509975 5mg $ 80.00 

FITC- #510204 5mg $ 98.00 

Rhodamine- #510205 5mg $ 98.00 

Texas Red - #510206 5mg $ 98.00 

For our complete line of lectins visit 


Custom tagging of lectins available 

Contact: tech@bio-world.com 

27

proteomics 

2 

Protein Extraction 

Protein Purification 

Protein Analysis 

Homo & Heterobifunctional Linkers 

flexARM (dPEG) Linkers 

Electrophoresis Tools 

Mass Spectrometry 

> 

accelerate: proteinresearch 

28 26

proteomics 


Cellbuster-A (Animal proteinXtraction) 

• An efficient cell lysis medium 

for animal cells for preparation 

of total cellular proteins. 

• Cell lysis facilitated by combination of proprietary 

detergent mixture. 

• The kit contains enough reagents for 50 medium scale cultures. 

• Includes 2M DTT & Protease inhibitor cocktail. 

#506152 1 Kit $ 148.00 

Cellbuster-B (Bacterial proteinXtraction) 

• Most efficient cell lysis medium 

for bacterial cells and 

preparation of total cellular proteins. 

• Efficient cell lysis facilitated by a combination of lysozyme & 

proprietary detergent mixture. 

• Enough reagent for 50 medium scale cultures. 

#506150 1 Kit $ 128.00 

DissolveAway (Inclusion bodies solubilization) 

New 

• For solubilization of inclusion 

bodies in protein expression 

studies involving bacteria. 

• Minimizes denaturation of proteins. 

#506140 100ml $ 37.40 

#506141 250ml $ 67.00 

• Increase efficiency & yield of your recombinant protein. 

• Use of FoamFree (cat #543100) is recommended in large preparations. 

Omni Homogenizer 

• Includes Omni TH Homogenizer, Omni Tip adapter, 

12 Omni Tip probes, convenient storage and accessory case. 

with Hard Tissue Probes #008530 1 Kit $ 746.00 

• Omni with 7mm x 110mm hard tissue Omni Tip probes 

with Long Probes 

For more homogenizers 

visit: www.bio-world.com 

#008529 1 Kit $ 746.00 

• Omni with 7mm x 110mm (long) deaggregation probes 

with Short probes 

#008528 1 Kit $ 746.00 

• Omni with 7mm x 65mm (short) deaggregation probes 

Proteomax Protein Extraction Systems 

Proteomax MP 

• For fractionation of highly 

enriched membrane proteins 

using a single step phase partition. 

• Membrane proteins are extracted with an efficiency greater than 90% 

with minimal cross-contamination from hydrophilic proteins. 

• Increases spot resolution for 2-D gels. 

Proteomax T 

• Broad-spectrum total protein 

sample preparation kit. 

• Extraction kit targeted for all 

proteins from animal cells including soluble & membrane proteins. 

• Contains buffers to solubilize difficult proteins. 

#515937 50 preps $ 195.00 

#515934 50 preps $ 89.50 

#515924 100 preps $ 149.00 

29 

proteomics Protein Extraction

proteomics 


30 

PerfectFocus-Protein Kits 

Perfect-Focus 2-D Gel Prep Kit 

• Designed to clean & 

#515940 1 Kit $ 149.25 

concentrate protein samples 

prior to running IEF/2-D gels. 

• Process is a simple wash & collection that yields a sample essentially free 

from non-protein agents. 

• Removes interfering agents such as detergents, salts, lipids, natural 

products, reductants, alkylation agents & EDTA. 

• Includes protein solubilization reagents ready for IEF application. 

• Sufficient for 50 preps. 

Protein Master Strategy 

• The Protein Master is a kit #078515 1 Kit $ 295.00 

designed to simplify protein 

purification and optimization 

procedures. 

• The kit guides the researcher 

step-by-step through various 

protein purification techniques, 

which include various protein 

precipitation-fractionation and 

chromatography techniques. 

• Supplied with all reagents needed 

to test various purification 

techniques. 

• No expensive equipment is 

needed. 

Cytoplasmic/Nuclear Protein Extraction Kit 

• Fractionation of cytoplasmic & 

#515936 50 preps $ 185.00 

nuclear proteins. 

• Supplied with a strong chaotropic extraction buffer to solubilize both 

cytoplasmic as well as nuclear proteins for 2-D gel analysis. 

Soluble & Insoluble Protein Kit 

• Allows selective preparation of 

soluble (hydrophilic) proteins & 

insoluble (hydrophobic) proteins. 

#515935 1 Kit $ 185.00 

• The soluble & insoluble proteins may be further fractionated. 

• Store Perfect-FOCUS kit at room temperature. 

• Kit contains: all reagents necessary for protein fractionation. 

Protein Alkylation Kit 

• Ultrapure grade lodacetamide 

#506176 1 Kit $ 74.40 

• Simply add appropriate amount 

of reagent solution for alkylation of SH-groups in proteins. 

• Minimizing re-oxidation of the competing thiol pairs. 

Signal Protein Extraction Kit 

• For fractionation of caveolinenriched 

membrane proteins, 

#515938 50 preps $ 126.30 

proteins rich in cholesterol, glycolipids, GTP-binding & GPI linked 

proteins & protein molecules involved in directing intracellular 

membrane traffic. 

Protein Reduction Kit 

• Water soluble, odorless, nontoxic, 

stable TCEP (Tris[2- 

#506174 100 preps $ 75.40 

carboxyethyl]phosphine hydrochloride) reductant. 

• Works over a wide pH range including lower acidic pH. 

• Reduces disulfide bonds in less than 5 minutes at room temperature. 

• Use of the TCEP reductant is compatible with the alkylation reaction of 

the SH-groups for 2-D gel analysis. 

Protein Reduction/Alkylation Kit 

• Improves resolution & 

#506172 100 preps $ 129.30 

prevents streaking of protein 

spots on 2-D gels. 

• Kit offers a simple two step method for reduction & alkylation of 

protein samples for 2-D gel analysis. 

• Disulfide bonds are reduced with a highly active & stable reagent TCEP 

(Tris[2-carboxyethyl]phosphine hydrochloride) followed by blocking of 

the thiols by alkylation.

FoamFree 

• Free-flowing dry powderspecifically 

developed for 

controlling foam in various 

biotechnology applications. 

#543100 

#543101 

50G 

100G 

$ 39.50 

$ 67.20 

• Prevents foaming during sonication or homogenization of cells & 

tissues for protein extraction, hybridization solutions during rocking & 

shaking & during fermentation of bacterial or yeast cultures. 

• Inert, non-toxic & non-metabolizable. Does not interfere with enzyme 

activity or assays. 

bioDetergents 

Test Kit 

• Six detergents with different 

properties for solubilization of 

membrane proteins. 

#730034 1 Kit $ 98.50 

• Contains: 10g CAPS, CHES, Deoxycholic Acid, 0.5g CHAPS, CHAPSO & 

N-Octyl-Glucopyranoside. 

proteomics 

Protease Inhibitors 

Antipain Dihydrochloride 

• MF: C 27 H 44 H 10 O 6 2HCl 

• A reversible cysteine and serine 

protease inhibitor of trypsin, 

papain and cathepsin A and B. 

• Slightly inhibits plasmin. 

Bestatin 

• MF: C 16 H 24 N 2 O 4 

• A competitive inhibitor of 

aminopeptidases, especially aminopeptidase B, leucine aminopeptidase 

and tripeptide aminopeptidase. 

• Not an inhibitor of carboxypeptidases. 

Chymostatin 

• MF: C 31 H 41 N 7 O 6 

• A reversible cysteine and serine 

protease inhibitor of a, b, g and 

d-chymotrypsin. 

#711001 5mg $ 35.50 

#711002 10mg $ 99.70 

#711003 5mg $ 52.47 

• Also inhibits papain and many other cysteine proteases. 

proteomics 

proteomics Protein Inhibitors 

Protein Extraction Kits 

Detergent-Out® Kit, DTG-100X 

• Simple, high performance 

#542551 1 Kit $ 142.30 

method for removing free 

detergents from protein solutions without significant loss or dilution. 

• Simply load the protein solution on the Detergent-Out ® column & spin. 

Detergent is retained by the column & the protein solution is eluted & 

collected in a small volume. 

• Designed for the removal of non-ionic detergents such as Triton-X100, 

NP-40, Tween & Sodium Deoxicholate. 

• Applications: 10 (each can be up to 2ml of protein solution) 

Detergent-Out ® Kit, SDS-300 - For SDS Removal 

• (See kit above for info) 

#542550 1 Kit $ 99.80 

E-64 

• MF: C 15 H 27 N 5 O 5 

• Soluble in DMSO 

#711004 5mg $ 68.95 

• An irreversible inhibitor of cysteine protease like papain, calpain and 

cathepsin B, H, and S. 

Pepstatin A 

• MF: C 34 H 63 N 5 O 9 

• Soluble in DMSO, MeOH 

• An aspartic acid protease 

#711005 

#711006 

5mg 

25mg 

$ 31.48 

$ 82.92 

inhibitor of pepsin, renin and cathepsin D. 

• Also an ihibitor of the HIV protease. 

31

proteomics 

Protein Kits 

1,10-Phenanthroline 

• MF: C 12 H 8 N 2 H 2 O 

• A metalloprotease inhibitor 

useful for chelating iron and 

other divalent metals. 

• Store at room temperature 

#711007 

#711006 

10mg 

25mg 

$ 31.50 

$ 117.00 

Trypsin Inhibitor, Soybean 

• Activity: 87,000 BAEE Units/mg 

protein 

• Soluble in H 2 O 

#711010 

#711011 

1g 

10g 

$ 50.39 

$ 358.13 

• A serine protease inhibitor specific for trypsin and trypsin-like proteases. 

PMSF (Phenylmethyl sulfonyl fluoride) 

• MF: C 6 H 5 CH 2 SO 2 F 

• MW: 174.19 

• CAS: 329-989-6 

• Purity: >99.0% 

#705619 

#705621 

5g 

25g 

$ 28.77 

$ 96.60 

• Most commonly used protease inhibitor. 

• Dissolve in isopropanol to make 100mm stock. 

• Store in desk at 70ºC use 1mM. 

Protease Inhibitor Cocktail I 

• Supplied as 100X solution 

• Benzamadine 

• Phenanthroline 

#508088 

#508089 

100 x 5ml 

100 x 1ml 

$ 139.00 

$ 31.00 

• Aprotinin 

• Storage; -20ºC 

• Pepstatin 

• Leupeptine 

proteomics 

Protein Purification Kits 

FOCUS - PhosphoRich 

• Ready to use kit allows 

enrichment of phosphorylated 

proteins. 

• It also provides a simple way 

to isolate phosphopeptides 

from complex samples. 

• The kit contains resin filled 

spin columns with binding 

capacity ~ 20mg of 

phosphorylated ovalbumin per 

column. 

Glycoprotein Isolation (GPI) Kit 

• The is Con A Kit is easy to use and 

contains all of the necessary 

components for isolation of most N-linked glycans. 

Con A Sepharose: 2ml settled resin supplied as a 50% slurry in buffer 

Binding/Wash Buffer: 10ml of a 5x stock solution 

Elution Buffer: 5ml 

Column Acessories Pack: 10 spin columns with bottom caps and 20 

collection tubes 

#078514 1 Kit $295.00 

• The resin columns supplied 

with the kit can be re-used, if 

regenerated and stored properly. 

#515554 1 Kit $ 190.00 

Protease Inhibitor Cocktail II 

32 

• Supplied as 100X solution 

• AEBSF 

• E-64 

• Bestatin 

• Leupeptin 

• Pepstatin 

#508091 100 x 5ml $ 139.00 

#508092 100 x 1ml $ 31.00 

• Storage; -20ºC 

> 

accelerate: proteinpurification

Protein Concentrator Kit 

Column Protein Concentrator Kit 

• Dilute protein solution 

#542560 10 assay $ 176.35 

(1µg/mL) can be quantitatively 

precipitated by using the Universal Protein Precipitation reagent 

followed by solubilization and centrifugation through a gel filtration 

spin column (provided). 

• Protein precipitation is not affected by the presence of detergents, 

chaotropic or other common laboratory reagents. 

• Method involves mixing protein solution with UPPA reagents, which 

quantitatively precipitate protein. 

• Suitable for concentrating protein for running gels, raising antibodies, 

protein purification, protein assays & other applications. 

• Not suitable for those proteins which may lose their biological activity 

when precipitated. 

Procipitate 

• Replaces the need for phenol 

#516207 10ml $ 115.00 

following restriction enzyme 

digests, ligations, labeling & #516209 25ml $ 184.00 

amplification reactions. 

• Removes phosphatases & nucleases as well as replaces phenol in nucleic 

acid isolations. 

• One step purification reagent developed from patented polymer 

bridging network technology. 

• Synthetic, water insoluble, anionic polyelectrolyte which selectively 

binds proteins in solution. 

• Supplied as a stable liquid suspension that is ready for use. 

Cleanascite 

• Lipid absorbent & clarification 

#516205 50ml $ 147.50 

agent for pretreatment of 

samples prior to further purification. 

• Suitable for ascites, serum & cell culture supernatants facilitating 

subsequent purification. 

• Virtually no cross reactivity with antibodies, hemoglobin, glycoproteins, 

proteoglycans, nucleic acids & most serum components. 

• Suitable for larger volumes of #078512 1 kit $ 78.50 

dilute protein solution. 

• This Kit has been specifically 

developed for concentration 

of those proteins that cannot 

be concentrated either by 

precipitation or other 

techniques. 

• Binds and immobilizes any 

protein in a low salt buffer 

between pH 3-10 (capacity ~ 

0.5mg protein/ml Protein 

Binding Resin). 

• The immobilized protein is 

spin-eluted in a small volume 

of specifically formulated elution 

buffer, giving several fold 

effective concentration. 

• Will concentrate a total of 4mg 

protein in either single or 

multiple procedures. 

• The kit is supplied with a 

proprietary spin column that may 

be regenerated and re-used once. 

• Recovery ~ 80% 

Dextropore Desalting Columns 

• Specifically designed to effectively separate proteins, complex 

carbohydrates & nucleic acids from smaller molecules like peptides, 

nucleotides, labels & salts. (Similar to Sephadex) 

• Kit Includes: 100 columns Dextropore (frit attached), collection tubes, 

2 racks of 200µl tips, 1 rack of 1000µl tips and Dextropore. 

G-10 #078502 100 $ 238.23 

G-15 #515915 100 $ 259.40 

G-25 #078504 100 $ 298.00 

G-50 

#078506 100 $ 298.00 

bioZorbant 

• Highly useful material for 

#475227 1kg $ 47.50 

concentration of proteins and 

other macromolecules and can be used for rapid removal of water 

from dialysis tubings. Absorbs 20 times water by weight. 

proteomics Protein Kits 

33

proteomics 

Protein Kits 

E-Z Protein Stabilizer 

New 

• E-Z Stabilizer is a cocktail of 4 

ingredients, each of which adds 

stability to purified enzyme/ 

protein & increases storage time while maintaining enzyme activity. 

• Supplied as a 10X solution. 


Empty Chromotography Columns 

• Appropriate columns for protein fractionation, affinity 

chromatography, desalting and other procedures are necessary for 

proteomic studies. 

• bioWorld offers a variety of analytical columns, 50/pack. 

A. Capped 0.8ml 

C. With collection tubes 

D. Spin column 0.5ml 

E. Capped 3ml column 

#042015 A $ 45.00 

B. Uncapped 0.8ml #042016 B $ 39.00 

For more columns visit: 


#543110 20ml $ 48.00 

#543111 50ml $ 87.20 

#042017 C $ 47.00 

#042018 D $ 49.00 

#042019 E $ 69.00 

New 

Colloidal Gold for Protein Assays 

Auraquant 

#515526 250ml $ 129.00 

Quantigold 

#515525 250ml $ 117.00 

• Fast, sensitive assay ideal for accurately detecting nanogram quantities 

of protein in solution. 

• Capitalizes on the affinity of protein to collodial gold creating a stable 

color, with maximum absorbance at 595nm. 

• Protein quantities can be extrapolated from the BSA standard curve, 

which is linear in the 5-200ng range. 

• Auraquant is about 30% more sensitive than Quantigold. 

• Quick & Easy: One-Step, One-Solution assay, can be completed in 

30 minutes. 

• Stable, accurate results readable up to 24 hours following completion. 

• Requires only minimal amounts of protein samples. 

• Materials required: Distilled/deionized water, micropipettes, 

1.5ml clear microfuge tubes & disposable 1.0ml micro-assay cuvettes. 

• Microplate format can be adopted 

• Stable for at least 6 months at 4˚C . Do not freeze. 

CB-Protein Assay 

• Coomassie dye-based protein 

#542320 500ml $ 85.50 

assay provides simple, rapid 

estimation of protein concentration & can be performed using as little 

as 1mg protein per sample. 

• Supplied ready-to-use with an easy to follow protocol that does not 

require pre-filtering or dilution. 

• Simply mix the protein solution with CB-Protein Dye & read optical 

density 5 minutes later at 595 nm 

• Compatible with reducing agents. 

• This assay is contradicted if the protein solution contains a high 

concentration of detergents. 

• Kit comes with Albumin Standard (2mg/ml) 

34 

proteinpurificationmadeeasy

Non-Interfering Protein Assay 

• Simple to follow protein assay 

#542300 500 assay $ 156.10 

overcomes interference of 

agents commonly present in protein solutions & shows no protein-toprotein 

variation. 

• Non-Interfering Protein Assay has been extensively tested to work in 

the presence of reducing agents, chelating agents, detergents, amines 

& numerous other agents. Not affected even if the protein is in 

strong extraction buffers. 

• Universal Protein Precipitating Agent (UPPA) removes 

interfering agents from protein solutions. 

• Any protein modifications (glycosylation, etc.) or the presence of 

normally interfering compounds do not affect this protein assay - you 

get reliable results every time from different samples. 

• Simple protocol yields excellent results in as little as 15 minutes. 

• For less than 1.5ml cuvettes, scale all reagents down. 

• Components: 2ml assay tubes, disposable cuvettes, Albumin 

protein standard. 

Bradford Protein Assay 

• Bradford Method utilizes Coomassie Brilliant Blue G-250 dye binding 

to an unknown protein & forming a complex that can be detected 

spectrophotometrically at 595nm. 

• Method of choice for protein quantitation. 

• Designed to quantitate 1 to 10µg/ml protein but can be scaled up to 

quantitate 10 to 100µg/ml simply by increasing the volume of the dye 

generating a standard curve in the 10 to 100µg/ml range. 

• Includes BSA standard 10mg/ml, 2ml) 

Protein Assay Kit #700600 1 kit $ 99.00 

• Sufficent for 1000 assays 

Dye Concentrate (5X) #700601 500ml $ 58.90 

Reagent Ready To Use #700602 500ml $ 28.50 

#700603 1L $ 47.50 


BCA Protein Assays 

#078516 1 Kit $ 87.00 

• A simple and sensitive 

colorimetric assay for the determination of protein concentration 

• Comes with two solutions plus a protein standard 

• The concentration dependent reaction reduces 

a blue color that is read at 562nm. 

• Compatible with many common buffers 

and detergents. 

• Can be used with both tubes and 

microplates. 

• Sufficient for 500 samples 

 

 

 

 

 

© 

Protein Standards 

Bovine Serum Albumin 

• Concentration: 2mg/ml 

• Prepared in saline buffer 

• Used as a standard in protein assays 

Ovalbumin 

• 2mg/ml concentration 

Gamma Globulin 

• 2mg/ml concentration 

• Used as standard in 

protein assays 

#542301 5 x 2ml $ 33.50 

#715080 5 x 2ml $ 23.50 

#542302 5 x 2ml $ 33.50 

• Simple protocol yields excellent results in as little as 15 minutes. 

> 


35

proteomics 

Protein Kits 

Dotmetric Protein Assay 

• Sensitive, economical & takes only 10 minutes. 

• Requires as little as 1µL of sample & has the sensitivity to detect as little 

as 0.5ng (BSA) protein. 

• Determine protein concentration by measuring the diameter of the 

protein spot with the dotMetric gauge supplied with each kit. 

• No protein to protein variation. 

• Uniquely tolerant of detergents and other common laboratory agents. 

• Resistant to Triton-X 100, TWEEN-20, NP -40, SDS, reducing agents 

such as ß-mercaptoethanol, DTT, Sugars, EDTA, Tris , Urea & 

Guanidine-HCl. 

• Can be used even if the protein is in SDS-PAGE gel loading buffer. 

• Suitable for Gel Loading Samples (SDS-PAGE), Total Cellular Protein 

& Crude Extract Cellular Fractions, Chromatography and Purification 

Extracts. 

• Each kit is supplied with the necessary reagents & test strips for 

assaying up to 300 samples. 

• Highly reliable (+/- 5% above 5µg/ml) determination of 

protein concentration. 

Protein Basic Kit 

#515552 300 preps $ 149.15 

Protein Kit w/ Tips & Board #515553 1 Kit $ 235.00 

OsmoCons Protein Folding Facilitators 

• A clear understanding of the 

energetics of osmolyte induced 

protein folding/unfolding has 

#506524 1 kit $ 98.50 

recently been established (Auton & Bolen, 2005 PNAS 102: 15065). 

• bioWorld offers a set of Osmocons, well-recognized osmolytes 

to assist in solvent-dependent cooperative protein folding and free 

energy changes. 

• Kit Contains: 

* 1M TMAO, 25ml 

* 1M Sorbitol, 25ml 

* 1M Sarcosine, 25ml 

* 1M Proline, 25ml 

* All reagents are filter sterilized 

TMAO (Trimethylamine- N-Oxide, dihydrate) 

• MF: C 3 H 9 NO*2H 2 O 

#078516 25ml $ 47.00 

• MW: 111.14 

#078517 50ml $ 73.00 

• CAS: 62637-93-8 

• 1M Sterile 

• Facilitates protein folding 

FoldRight Kit 

• Designed to optimize protein 

#543400 1 Kit $ 247.00 

folding procedures. 

• Based on a proprietary combination of denaturing agents for 

solubilization of inclusion bodies and the foldright protocol based on 

rapid dilution of denatured protein into folding mixes. 

• Folding mixes are based on proven folding agents 

Polyethylene Glycol, Cyclodextrine, ND SB-201 detergent, and 

Ox-Re agents (oxidizing-reducing agents). 

• Foldright kit offers a simple-to-follow protocol to quickly optimize 

folding protocol for proteins. 

• See also Osmocons (below). 

Proline 

• MF: C 5 H 9 NO 2 

• MW: 115-13 

• CAS: 147-85-3 


• Stabilize proteins 

#078522 50ml $ 27.50 

#078523 100ml $ 43.00 

36

Sarcosine (N-Methylamino-acetic or N-Methylglycine) 

• MF: C 3 H 7 NO 2 

• MW: 89.09 

• CAS: 107-97-1 


• Facilitates protein foldinig 

Sorbitol 

• MF: C 6 H 14 O 6 

• MW: 182.17 

• CAS: 50-70-4 


Urea (8M ) 

• MF: CH 4 N 2 O 

• CAS: 57-13-6 

• MW: 60.06 

• Solubilize proteins by denaturation 

#078520 25ml $ 34.00 

#078521 50ml $ 47.00 

#078518 50ml $ 34.00 

#078519 100ml $ 49.00 

#705830 100ml $ 34.00 

#705831 500ml $ 65.00 

Trypsin-Digestion Mix 

• For efficient digestion of 

proteins with trypsin. 

• TPCK modified Mass-Spec. grade 

#515947 100 preps $ 77.50 

-Used with In-Gel Protein Digestion Kit 

Proteomics Protein Controls 

• Protein preparations specifically prepared for producing 2-D gels as 

controls for standardizing electrophoresis. 

• Protein preparations are reduced & SH-groups substantially blocked 

by alkylation. 

• Free from reductants, alkylation agents & other common 

laboratory agents. 

• Low conductivity 

• Supplied as dry protein pellets in ready to use form, simply rehydrate 

pellets in an appropriate buffer. 

Animal (Mouse Liver) #515942 2 x 2mg $ 67.00 

E.coli #515943 2 x 2mg $ 47.00 


In-Gel Protein Digestion 

• Simple, reliable method of in 

#515946 100 preps $ 109.80 

gel trypsin digestion of protein, 

in gel spots & bands for Mass Spec analysis. 

• Excise/remove protein spots/bands & wash in SilverOUT to remove 

silver ions followed by incubation in our proprietary Trypsin-Digestion 

Mix which ensures efficient protein digestion. 

• Digested peptides are extracted with Pep-Extract, a high diffusion 

peptide extraction buffer. 

• Supplied with sufficient reagents for extraction of 100 protein bands. 

Plant (Arabidopsis) #515944 2 x 2mg $ 57.00 

Yeast #515941 2 x 2mg $ 59.00 

Standard Set (all 4) 

#515945 2x2mg each $ 146.85 

37

proteomics 

Protein Kits 

38 

Antigen:Antibody Dissociators 

Acidic Dissociator, pH2.5 

• Includes Elution and Neutralize 

buffers. Just add predetermined 

#650161 1 Kit $ 49.50 

quantity to elution buffer to neutralize antibodies and prevent 

denaturation. Also includes 

Neutral Dissociator, pH 7.0 

• To dissociate your antigenantibody 

complex in a column 

at neutral pH. 

#650160 50ml $ 29.50 

• Dialyze to desalt after dissociating (under non-denaturing conditions) 

with neutral dissociator. 

HisTalon Affinity Purification Kit 

• Polyclonal antibodies (Rabbit) 

#600059 2ml $ 249.00 

against poly-Histidine are 

immobilized on Sepharose to prepare a column for purification of 

His-tagged proteins using affinity chromatography. 

• Bound protein can be eluted using denaturing or 

non-denaturing conditions. 

• Contains poly-His antibody bound Sepharose, column & elution 

buffers (Denaturing and non-denaturing elutions). 

HisTalon HRP-C 

• Nickel-NTA horseradish 

#600051 10 blots $ 247.00 

peroxidase (HRP) enzyme 

conjugate binds directly to the His-Tag protein and is detected using 

chromagenic substrates, saving a significant amount of time in Western 

Blot analyses. 

HisTalon HRP-L 

• Nickel-NTA horseradish 

#600050 10 blots $ 298.00 

peroxidase (HRP) enzyme 

conjugate directly binds to the His-Tag protein and is detected using 

intense chemiluminesence enhancing signal, saving a significant 

amount of time in Western Blot analyses. 

HisTalon AP-C 

• Nickel-NTA alkaline phosphatase 

#600049 10 blots $ 295.00 

(AP) enzyme conjugate binds 

directly to the His-Tag protein and is detected using chromagenic 

substrates, saving a significant amount of time in Western Blot analyses. 

QuantaLite 

• Up to 10-fold more sensitive #500617 1 Kit $ 265.00 

than ECL Plus. 

• High performance blocking agent and substrate included in the kit, 

enable detection of low-abundance proteins. 

• Ideal for use in situations where the primary antibody is in short supply 

or expensive to produce. 

• Easy-to-use protocol similar to ECL and ECL Plus. 

• Compatible with nitrocellulose and PVDF membranes, as well as both 

film and CCD imaging methods. 

Quick Immuno Detect Kit 

• For rapid detection of antigens 

#506151 1 Kit $ 78.60 

in any purification protocol, 

apply 1µL of sample to the strips provided, allow the strips to react 

with your primary (rabbit) antibody for 5 minutes, then apply the 

Quick-Immuno-Detect reagent provided & watch for the developing 

color change. 

• Kit contains sufficient reagents to detect 500, 1µL aliquots of antigen in 

any sample without the interference of salts & buffers. 

Western Stripper 

• Remove antibodies from a 

western blot and reprobe the 

same blot with a second antibody. 

#650100 1 kit $ 64.00 

• Contains Ab stripping buffer and neutralizer for reprobing. 

• Not effective with chromogenic substances.

QuantaLeap Signal Amplification System 

FireTag-HRP Conjugation Kit 

New 

• This kit provides a flexible-octoantennary 

PEG linking agent with 

#506190 1 Kit $ 245.00 

terminal active groups for the attachment of bio-molecules of your 

choice. This can be used for the conjugation of multiple enzymes 

such as peroxidase or alkaline phosphatase to ligand. Or, for creating 

multiple antigen complexes as immunogens. It is also useful for 

enhanced presentation of a ligand on a chromotographic support to 

create highly efficiant ligand attached bioaffinity 

matrix via a flexible linker. 

• Sufficient for 10 reactions 

Maleimide 

Maleimide 

Maleimide 

Maleimide 

NHS 

Maleimide 

Maleimide 

Maleimide 

Maleimide 

New 

• FireTag-HRP Conjugation Kit is #600047 6 x 0.1 mg rxn $ 245.00 

based on well-established 

#600048 2 mg HRP $ 178.00 

periodate chemistry, yielding 

consistent and reproducible protein conjugates. This kit contains all 

reagents required to conjugate your protein or antibody, including 

buffers and activated HRP and requires no activation or purification. 

Sufficient reagents are provided to conjugate 6 samples, each 

containing up to 0.1 mg of antibody or protein. 

• Modified HRP, SFB Linker and AP Conjugation Buffer (5X). 

FireTag-Activated HRP is also available as a stand-alone reagent. 

See catalog # 600048 

A 

O 

O 

N N H H 

N O 

N N 

H 

B 


SANH-modified Biomolecule A 

(Hydrazine) 

H 

SFB-modified Biomolecule B 

(Aldehyde) 

FireTag-AP Conjugation Kit 

Conjugation 

(acetone) 

O 

• FireTag-AP Conjugation Kit 

enables antibody or protein 

labeling with alkaline 

#600045 3 x 0.1 mg rxn. $ 275.00 

#600046 1 mg AP $ 198.00 

A 

O 

N 

H 

N 

H 

N 

O 

N 

H 

B 

phosphatase (AP) in less than 3 

hours, saving you several hours over standard maleimide/thiol methods. 

AP is supplied ready-to-use, preactivated and requires no activation or 

purification. Sufficient reagents are provided to conjugate 3 samples, 

N 

H 

A-B Bioconjugate 

(Hydrazone) 

each containing up to 0.1 mg of antibody or protein. 

• Contains: 

Conjugation of SANH and SFB Modified BioMolecules 

New 

Modified AP, SFB Linker and AP Conjugation Buffer (5X). 

FireTag-AP is also available as a stand-alone reagent. Cat# 600046 

novel conjugation & signal amplifyers 

39

proteomics 

Protein Kits 

40 

New 

Intense ChemiLuminescence (ICL) Kit 

for Western Blots 

Intense Chemiluminescence (ICL) Kit 

for ELISA 

New 

ICL Western Blot kit is a two-component substrate that contains a stable 

luminol solution with an enhancer and a stable peroxide solution. 

• Longer light emission - strong 

light emission over a working 

day allows you to make several 

exposures 

• Picogram sensitivity - highly sensitive for the rapid development of a 

wide range of protein levels 

Kit contains: 

• Luminol/Enhancer, 250 ml 

• Stable Peroxide Buffer, 250 ml 

For greater sensitivity in ELISAs or any other solution-based assay use ICL 

(intense chemiluminescent) substrate. ELISAs can take place in either a test 

tube or a microplate and are quantified by measuring relative light units 

(RLU) in a luminometer. ICL substrate was developed for researchers who 

need high sensitivity at an economical price. 

• Immediate light generation - 

intense signal is produced 

immediately at room temperature 

• High signal:noise ratio - minimal background 

• Low picogram sensitivity 

• Room temperature storage - a consistent product with ambient 

shipping and no need to store at 4°C 

• Consistent performance of the working solution over an 8-hour period 

• Flexible - signal can be read in black or white opaque plates 

• Emits light at 425 nm 

Includes: 

• Luminol/Enhancer Solution 50 ml 

• Stable Peroxide Buffer 50 ml 

#506191 1 Kit $ 175.00 

 

#506192 1 Kit $ 275.00 

Prionex, BSA Alternative 

New 

• Used as a protein stabilizer, 

blocking agent, protectant and 

excipient. 

• Prionex is freely soluble in water, 

dilute electrolyte solutions, glycerol, DMSO, and ethanol at 

concentrations below 75% and ammonium sulfate solutions below 20% 

saturation. 

NAP-Blocker 

#705101 25ml $ 47.00 

#705102 100ml $138.00 

A new non-animal blocking protein preparation for immuno-assays. For 

improved assay sensitivity, clean background, and a high signal-to-background 

ratio, bioWorld has developed NAP-Blocker - a blocking agent 

containing non-animal proteins. Because NAP-Blocker is a nonanimal 

protein you can be absolutely sure that there is no cross reaction with your 

animal source antigens, or primary and secondary antibodies. NAP-BLOCK- 

ERTM is free from biotin and other cross-reacting agents present in animal 

source blocking agents. NAP-Blocker ensures uniform blocking without 

nonspecific binding. Simple to use and better results than milk powder 

preparations. 

in PBS 

in TBS 

• Supplied as a premade [2X] 

solution; simply dilute with any 

buffer and block nitrocellulose or PVDF membranes. 

#462157 500ml $ 56.00 

#462158 1L $ 98.50 

• Blocking agent for immuno-detection assays such as Western blots, dot- 

blots, and ELISA. 

#462159 500ml $ 56.00 

#462160 1L $ 98.50 

For ready protein blots 

visit: www.bio-world.com

proteomics 

Heterobifunctional Crosslinkers 

SANH and SBF Linkers 

SANH and SFB (succinimidyl 4-formylbenzoate) will modify proteins by 

incorporating hydrazine and benzaldehyde moieties, respectively, in a 

single step. The alkyl hydrazone protecting group on SANH readily 

hydrolyzes in acidic or neutral pH (4.5-7.4) and the liberated hydrazine 

moiety reacts with the aldehyde modified biomolecules to produce stable 

hydrazone conjugates (see below). Unlike the classical maleimido/thiol 

linkers, proteins modified with either hydrazines or aldehydes are very 

stable for months. 

SFB Linker 

• SFB (Succinimidyl 4- 

formylbenzoate) is a 

#600044 20mg $65.00 

heterobifunctional crosslinker used to modify amine-containing 

biomolecules or surfaces to directly incorporate benzaldehyde moieties. 

EMCH (e-Maleimidocaproic acid hydrazide) 

• MF: C 10 H 15 N 3 O 3 *CF 3 CO 2 H 

#507013 50mg $ 120.00 

• MW: 339.27 

• MP: 104-105C 

#506997 100mg $ 198.00 

• Spacer length: 11.8Å 

• Sulfhydryl and carbonyl reactive heterobifunctional crosslinking agent 

• Useful for preparing immunoconjugates of doxorubicin 


MBS1(m-Maleimidocaproic N-hydroxysuccinimide) 

• MF: C 15 H 10 N 2 O 6 

• MW: 314.25 

• CAS: 58626-38-3 

• MP: 175-177C 


#507016 100mg $ 54.00 

• Sulfhydryl and amino reactive heterobifunctional protein crosslinking 

agent. 


proteomics Protein Linkers 

SANH Linker 

• SANH (Succinimidyl 6- 

#600043 10 mg $145.00 

hydrazinonicotinamide 

acetone hydrazone) is heterobifunctional crosslinker used to modify 

amine-containing biomolecules or surfaces to directly incorporate 

2-hydrazinopyridine moieties. 

A 

NH O B A 

N 

B 

2 

H + 

Sulfo-SMCC (Sulfosuccinimidyl 4-[N-maleimidomethyl] 

cyclohexane-1-carboxylate) 

• MF: C 16 H 17 N 2 O 9 SNa 

• MW: 436.37 

• CAS: 92921-24-9 

• Sulfhydryl and amino reactive heterobifunctional crosslinking agent. 



 

 

 

#507033 100mg $ 120.00 

#506996 500mg $ 547.00 

 

 

 

 

 

 

 

 

N 

N 

SANH 

SFB 

-H 2 O 

H 

H 

H H 

HYDRAZINE ALDEHYDE HYDRAZONE 

(See page 39 for more details) 

41

proteomics 

Protein Linkers 

Sulfo-SIAB (N-Sulfosuccinimidyl[4-iodoacetyl]aminobenzoate) 

• MF: C 13 H 10 N 2 O 8 INa 

• MW: 504.20 

• CAS: 58626-38-3 

• MP: 175-177C 


• Sulfhydryl and amino reactive 

heterobifunctional protein crosslinking 

agent. 

• Water soluble analog of SIAB 

proteomics 

Homobifunctional Crosslinkers 

• MW: 309.28 

• Cleavable, bifunctional 

imidoester crosslinker 

• Water soluble 

#507021 100mg $ 120.00 

 

 

 

 

 

 

DTBP (3,3’-dithiobis-propionimidate HCl) 

• MW: 660.45 

• Water soluble and cleavable 

• Amine reactive 

 

#507010 1g $ 70.00 

• Cleaved by thiols and reducing agents. 

Sulfo-EGS [Ethylene glycol bis (sulfosuccinimidylsuccinate)] 

#506993 50mg $ 94.50 

 

 

 

Sulfo-DST (Disuccinimidyl tartarate) 

• MW: 584.3 

• Primary amine reactive 


• Periodate cleavable 

Sulfo-BSOCOES 

Bis[2-(Sulfosuccinimidooxycarbonyloxy) ethyl] Sulfone 

• MW: 640.5 


• Water soluble and base cleavable 

proteomics 

flexArm (dPEG) Linkers 

#506994 100mg $ 104.00 

#506992 100mg $ 104.00 

flexArm is a descrete size polyethylene glycol spacer which is hydrophilic. 

flexArm NHS-(dPEG) 4 - Biotin 

• Adds aspecial arm for 

rapid and tight avidin/ 

streptavidin binding. 

• dPEG spacer is 

non-immunogenic. 

• Ultra high purity 

• Reactivity: amine 

#789245 4x5mg $ 70.00 

#789246 50mg $ 95.00 

#789247 1g $ 745.00 

• Solubility: high water soluble 

• Spacer Length: 19.2 Å 

• MW: 588.67 

• Single molecule 

DTSSP [3,3’-dithiobis-(sufosuccinimidyl propionate)] 

• MW: 608.5 


#506995 100mg $ 104.00 


New 

O 

HN 

NH 

S 

O 

N 

H 

O 

O 

O 

O 

O 

O 

N 

O 

O 

• Cleavable by disulfide reduction. 

42 

enhance solubility of your peptide/protein 

by using flexARM linkers.

flexArm MAL-(dPEG) 4 NHS ester 

New 

New 

• Hydrophilic (water soluble) and 

non-immunogenic spacer. 

• Spacer may reduce or eliminate 

problems with aggregation and 

immunogenicity 

• Hydrophilic dPEG spacer is 


• Reactivity I: amine 

• Hydrophilic/water soluble and 

non-immunogenic spacer. 

• Spacer may reduce or eliminate 

problems with aggregation and 

immunogenicity. 



• Reactivity I: amino 

• Reactivity II: thiol/sulfhydryl 


O 

N 

O 

O 

N 

H 

#789248 100mg $ 195.00 

#789249 1g $ 995.00 

• Reactivity II: sulfhydryl/thiol 


• Spacer Length (atoms): 22 

• MW: 513.50 

flexArm MAL-(dPEG) 12 NHS ester 

O 

N 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

N 

O 

#789250 100mg $ 220.00 

#789251 1g $1245.00 

• Solubility: water soluble; also very 

soluble in various organic solvents 

including DMAC and methylene 

chloride. 


• Spacer Length: (atoms): 46 

• MW: 265.30 

O 

O 

O 

O 

O 

O 

H 

N 

O 

O 

N 

O 

O 

New 

flexArm Amino-(dPEG) acid 

• Unprotected amino acid for those who prefer the potential simplicity of removing one less 

protecting group. 

#789252 #789253 #789254 100mg 500mg 1g $ 95.00 

$ 367.00 

$ 545.00 

• Introduce into peptides and related compounds to 

increase water solubility to 

reduce or eliminate 

aggregation pproblems. 

• Hydrophilic dPEG spacer is non-immunogenic. 

• Solubility: Zwitterionic, 

highly water soluble; soluble in 

methylene chloride to at least 

40 mg/ml. 

• Spacer Length:18.0 Å 

• MW: 265.30 


- O O 

O 

O 

O 

NH3 + 

O 

O 

O 

O 

O 

flexArm N-Fmoc-amido-(dPEG) 4 acid 

• N-Fmoc protected spacer for #789257 100mg $ 145.00 

peptide synthesis. 

#789258 1g $ 745.00 

• Introduce into peptides to 

increase water solubility and to 

reduce or eliminate 



• Solubility: water soluble 



• MW: 487.54 



• Ultra high purity. 

H 

O N 

O 

O 

O 

O 

O 

H 

proteomics Protein Linkers 

flexArm Amino-(dPEG) 8 acid 

• Same as # 789252 but with 

longer spacer 

#789250 100mg $ 220.00 

#789251 1g $1245.00 

New 

O 

O 

New 

• Length 32.2 Å 

• Atoms 28 

• MW: 441.51 


For protocols visit: 

www,bio-world.com 

43

proteomics 

Protein Linkers 

flexArm N-Fmoc-amido-(dPEG) 8 - acid 

New 

New 

• N-Fmoc protected spacer for 

peptide synthesis. 

• Introduce into peptides to 


reduce or eliminate. 





• Reactivity: amino using 

carbondiimide 

flexArm N-Fmoc-amido-(dPEG) 12 - acid 

• Protected spacer for peptide 

synthesis. 

• Introduce into peptide to 


reduce or eliminate. 

aggregation problems. 




• Reactivity: amino 

HO 

HO 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

#789259 100mg $ 195.00 

#789260 1g $ 995.00 

• Solubility: water soluble; also 

#789261 100mg $ 195.00 

#789262 1g $ 995.00 

• Solubility: water soluble; also 

soluble in various organic 

solvents including DMAC and 

methylene chloride. 



• MW: 839.96 


O 

soluble in various organic 

solvents including DMAC and 

methylene chloride. 



• MW: 663.75 


O 

O 

O 

O 

O 

O 

O 

O 

O 

H 

N 

O 

H 

N 

O 

O 

O 

flexArm NHS-m-(dPEG) 12 

New 

• Enables discrete MW pegylation #789263 100mg $ 170.00 

to almost 100g/mol 

#789266 500mg $ 590.00 

• Produces compounds with 

increased water solubility and 

reduced aggregation, or 

surfaces with reduced nonspecific 

binding. 

• Produces stable amide bonds 


• High purity. 




• MW: 685.75 



H 2 C 

O 

O 

N 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

O 

flexArm NHS-m-(dPEG) 4 

New 

3 O 

• Enables low MW pegylation of 

small molecule, drugs and 

related compounds. 

• Increase water solubility and 

reduced aggregation, or 

produces surfaces with reduced 

non-specific binding. 

• Produces stable amide bonds 



H 2 CO 

3 

#789263 100mg $ 170.00 

#789267 500mg $ 590.00 





• MW: 333.33 


o 

o 

o 

o 

o 

N 

o 

o 

44 

 

 

 

 

 

©

GeBAflex-Tube 

Mini Dialysis 

• GeBAflex-Tube defines the 

next generation technology in 

dialysis of small-volume samples 

based on a single-use tube. 

• Removal of Ethidium bromide, 

Urea & detergents. 

• Minimum sample size: 50µl 

• Volume Range: 10-250µl 

• Molecular Weight cut off: 3500, 6000-8000 & 12000-14000 Da 

• Purity: Protease, RNase, DNase & PCR free 

• Membrane: Ultra-clean, sulfur & heavy metal free, EDTA treated 

• Single use unit, single step process, no need for cleaning or autoclaving. 

• Kit includes: GeBAflex-tubes, Floating Rack, Handbook 

Midi Dialysis 


• (see Mini Dialysis for info.) 

Maxi Dialysis 


• (see Mini Dialysis for info.) 

Midi Electroelution 


• Gel size: 0.5 x 1.0cm 

• Kit includes: Floating Rack, 

Supporting Tray, 10/20ml TCA, 

5ml MS buffer, 5ml KAC 

buffer, Handbook 

• (see Mini Full Kit for info.) 

#506181 10 tubes $ 32.50 

(6000) 

#506183 30 tubes $ 63.00 

(6000) 

#506182 10 tubes $ 32.50 

(12000) 

#506184 30 tubes $ 63.00 

(12000) 

#506162 10 tubes $ 46.50 

(3500) 

#506163 30 tubes $ 130.00 

(3500) 

#506164 10 tubes $ 46.50 

(6000) 

#506165 30 tubes $ 130.00 

(6000) 

#506166 5 tubes $ 45.00 

(3500) 

#506167 15 tubes $ 90.00 

(3500) 

#506169 15 tubes $ 90.00 

(6000) 

#506170 5 tubes $ 45.00 

(12000) 

#506171 15 tubes $ 90.00 

(12000) 

#765262 10 tubes $ 63.00 

(3500) 

15 tubes $ 176.40 

#765263 (3500) 

#765264 30 tubes $ 94.50 

(3500) 

#765265 10 tubes $ 63.00 

(6000) 

Mini Full Kit 

• Extraction of DNA, in vitro 

transcribed SRNA, protein DNA 

& protein RNA from agarose or 

polyacrylamide gels for 

cloning or sequence analysis. 

• Extraction ofRNA from 

polyacrylamide gel for structure 

analysis, splicing & other biology experiments. 

• Will fit in most electro elution units available. 

• RNase, DNase & PCR product free 

• Single use unit, single step process. 

• Membrane: Sulfur & heavy metal free 

• Kit includes: Floating Rack, Supporting Tray, 2.5/7.5ml TCA, 250/750ml 

MS buffer, 300/900ml KAC buffer, Handbook 

Midi Full Kit 

• Kit includes: Floating Rack, 

Supporting Tray, 10/20ml TCA, 

5ml MS buffer, 5ml KAC 

buffer, Handbook 


Maxi Kits 

• Kit includes: GeBAflex-tube 

5/15, Supporting 

Tray, Handbook 


• No buffers included 

#506177 10 tubes $ 36.00 

(6000) 

#506178 30 tubes $ 72.00 

(6000) 

#506179 10 tubes $ 36.00 

(12000) 

#506180 30 tubes $ 72.00 

(12000) 

#506142 10 tubes $ 67.50 

(3500) 

#506143 15 tubes $ 101.25 

(3500) 

#506144 20 tubes $ 126.00 

(3500) 

#506145 30 tubes $ 189.00 

(3500) 

#506156 10 tubes $ 67.50 

(6000) 

#506157 15 tubes $ 101.25 

(6000) 

#506158 20 tubes $ 126.00 

(6000) 

#506159 30 tubes $ 189.00 

(6000) 

#506146 5 tubes $ 49.50 

(3500) 

#506147 15 tubes $ 101.25 

(3500) 

#506160 5 tubes $ 49.50 

(6000) 

#506161 15 tubes $ 101.25 

(6000) 

#506148 5 tubes $ 49.50 

(12000) 

#506149 15 tubes $ 101.25 

(12000) 

45 

proteomics Electroelution Tools

proteomics 


proteomics 


bioPAGE PreCast Polyacrylamide Gels 

• Variety of gradients to provide a wide range of 

8% 

10% 

12% 

4-20% 

8-16% 

8% 

10% 

12% 

4-20% 

8-16% 

8% 

10% 

12% 

flexibility. 

• 10 gels per box, 10 wells per gel 

• 4% stacking layers with preformed wells. 

• 4 month shelf life from manufacture date 

• 100% reproducibility 

• Tris Glycine buffer gel 

Acrylamide cat. # well vol. price 

8-16% 

#519100 50µl $ 85.00 

#519101 50µl $ 85.00 

#519102 50µl $ 85.00 

#519103 50µl $ 85.00 

#519104 50µl $ 85.00 

#743115 30µl $ 85.00 

#743118 30µl $ 85.00 

#743120 30µl $ 85.00 

#743116 30µl $ 85.00 

#743117 30µl $ 85.00 

#743121 25µl $ 85.00 

#743122 25µl $ 85.00 

#743123 25µl $ 85.00 

#743125 25µl $ 85.00 

Save Time! 

Use Ready Gels 

omniPAGE Electrophoresis Systems 

Mini 

Maxi 

Modular System 

Blotting Unit 10 x 10cm 

• omniPAGE Mini is constructed 

using the latest injection molding techniques. 

Acrylamide Starter Kit 

• For protein gel electrophoresis. 

• Biotechnology Grade. 

#031350 1 set $ 496.00 

#031360 1 set $ 695.00 

#031349 1 set $ 675.00 

#031348 1 set $ 348.00 

• The unit incorporates a sealing system, which is compatible with all 

major types of 8 x 10cm and 10 x 10cm precast gels. 

• The omniPAGE mini gel system requires low buffer volumes, and offers 

rapid set up cooling and the ability to run up to 4 gels. 

• Set includes: 2 pack of notched glass plates, 2 pack of regular glass 

plates with bonded 1mm spacers, 1 dummy plate, 2 sets of 12 well 

combs (1mm thick), 1 casting base and casting mat, 1 cooling rack and 

1 set of cables. 

• Plate dimensions: 10cm x 10cm, Gel dimensions: 7.5cm x 8cm 

• Buffer volume: Minimum: 250 ml; Maximum: 1200 ml 

For more details visit: 


• Contains: Acryl/Bis 37.5:1 (40%) 100ml, Ammonium Persulfate 25g, 

TEMED 25ml, TG-SDS 10X Buffer 2 pk. Sufficant for 50 mini gels 

• Storage: 4ºC 

• Save time, enhance productivity 

#740007 1 Kit $ 90.50 

46 

Wearing gloves is highly recommended when handling kit contents

omniPAGE Maxi System 

• 0mniPAGE Maxi combines ease 

#031360 1 set $ 696.00 

of use features with high 

resolution separations. 

• Ultra soft silicone seals guarantees trouble free glass plate loading and 

gel casting. 

• Set includes: 2 pack of notched glass plates, 2 pack of regular glass 

plates with bonded 1mm spacers, 1 dummy plate, 2 pack of 24 well 

combs (1mm thick), 1 casting base and casting mat, 1 cooling rack and 1 

set of cables. 

• Plate dimensions: 20cm x 20cm 

• Gel dimensions: 16cm x 17.5cm (W x L) 

• Buffer volume: Minimum: 1200 ml; Maximum: 5600 ml 

True Blue Protein Markers 

• Electrophoresis protein standards provide the sharpest, most uniform 

bands on the market. 

• Offers prestained low, mid & high molecular weight range kits with 

distinctly blue bands that yield a reliable & constant PAGE pattern. 

• Each protein is custom stained & calibrated to eliminate any frustrating 

anomalous mobility. 

• Presolubilized with one year shelf life. 

Low Range kit 

• Includes: Insulin, Aprotinin, 

#743000 500µL $ 114.15 

Lysozyme, Tripsin Inhibitor, & 

Carbonic Anhydrase. 

Mid Range kit 

• Includes: Cytochrome C (12,512 

#743001 500ML $ 123.50 

daltons), Lactate 

Dehydrogenase (36KDa), Beta-Lactoglobin (184KDa), Carbonic 

Anhydrase (29KDa), Ovalumin (43KDa), Glutamate 

Dehydrogenase (55KDa), Phosphorylase b (95KDa). 

High Range Kit 

• Includes: Myosin (205kDa), 

#743004 500ML $ 130.00 

Phosphorylase (97.4kDa), 

Bovine Serum Albumin (68kDa), Ovalbumin (43kDa), Carbonic 

Anhydrase (29kDa), Beta-Lactoglobulin (18.4kDa), & 

Lysozyme (14.4kDa). 

Gel Drying Frames and Films 

Complete Gel Drying Systems #408503 15 x15cm $ 59.60 

• Bio Gel-Drying System allows #408501 24 x24cm $ 98.70 

fast drying of gels because both sides can be exposed to air. The frame 

can be placed in an incubator up to 50°C for even faster drying. 

• Supplied with 2 sets of frames and a plate with 50 gel drying 

membranes. The system allows fast drying of a single gel or normal 

drying of two gels at one time. 

• Eight side clips also included. 

• Ideal for drying mini gels. 

Standing Gel Drying Frames #251500 6” x 6” $ 14.50 

• Vertical stand designed for rapid economical drying of electrophoresis 

gels. Inexpensive but effective system is ideal for easy dryinig of gels 

and for teaching laboratories. 

• Use Crack Free Solution (catalog #409504) to prevent cracking of gel 

during drying 

Flat Gel Drying Frames #251502 6” x 6” $ 12.70 

• Designed for rapid economical #251501 7” x 9.5” $ 19.50 

drying of electrophoresis gels. 

#251503 9.5” x 12” $ 25.00 

Inexpensive but effective system 

is ideal for the occasional users 

#408504 24cmx24cm $ 26.50 

and teaching laboratories. 

• Supplied as one pair of frames and one flat sheet. 

Gel Drying Films #408497 5.5” x 5.5” $ 17.60 

• This item is part of the gel 

#408502 7” x 9” $ 28.50 

drying system (Cat# 408501 

and 408503) that allows gels 

to dry quickly and easily between 

#408500 10” x 12” $ 32.67 

two sheets of cellophane without the use of expensive heat/vacuum 

gel dryers. 

• 50 sheets of pre-cut cellophane. 

47 

proteomics Protein Stains

Gel Plate Drying Rack 

proteomics 

• A sturdy, A-shaped rack that 

holds 12 glass electrophoresis 

#220721 1 rack $ 27.00 

plates vertically for quick drying and 

convenient access. 

• Can also hold Gel Drying Frames 

and Ready Gels Cassettes. 

Quick Reversible Stain 

• A single step, 5-minutes 

#742850 25 mini gels $ 110.00 

protocol for staining protein in 

SDS-Page gels. 

No destaining necessary. 

• Based on copper ions and 

produces colorless images of a band against a semi-opaque background. 

• This stain has greater sensitivity than Coomassie Blue. 

• Ideally suited for transfer or elution protocols where fixing of the 

protein bands is not required. 

Protein Stains 

Ponceau S 

• MF: C 22 H 12 N 4 Na 4 O 13 S 4 

• MW: 760.58 

• Protein Stain 

Coomassie Brilliant Blue G-250 

• CAS 3104-58-1 

• MW 854.04 

• Protein electrophoresis dye 

used in SDS-PAGE. 

#705844 50G $ 26.88 

#705845 100G $ 42.00 

• Also used for protein concentration determination by Bradford method. 

• Ultra Pure Grade. 

• Storage: RT 

#742073 10g $ 17.85 

#742077 25g $ 34.65 

#742081 50g $ 63.00 

New 

• The kit contains reagents for 25 mini gels. 

Express Coomassie Stain 

R-250 

#742830 100ml $ 69.50 

G-250 

#742831 100ml $ 69.50 

• Express Coomassie Stain detects as little as 15ng of protein and gives 

complete band detection within 50 minutes. 

• Ideal for densitometry and able to be stained with silver 

• This stain can be used more than once. 

• Express Coomassie is a colloidal suspension of Coomassie Blue that 

preferentially binds to protein and eliminates the unwanted destaining 

step. 

SeeBand Protein Stain 

• Blue Straining gel similar to 

#742060 500ml $ 30.00 

48 

Coomassie staining for protein 

gel electrophoresis. 

• Allows electro elution of a desired protein with high recovery yield 

• No denaturative material affecting the protein during staining. 

• Guarantees protein staining in polyacrylamide gels with only slight 

fixation of the protein to the gel. 

• No need for multi-step washing. 

• Washing uses water only 

• Storage at 4ºC. Shelf life 12 months 

RAPIDstain

RAPIDstain 

An ultra sensitive and ready-to-use protein stain based on Coomassie dye. 

No mixing or preparation is involved. No fixation step is needed. Simply 

pour RAPIDstainTM on your gel, and bands develop within 5-10 minutes 

and reaches a maximum visibility in 60 minutes. You can monitor the bands 

developing directly in the staining tray. 

#462155 500ml $ 37.00 

#462156 1L $ 65.00 

• Only stains protein, leaving a crystal clear background resulting in high 

and visibility. The gels require no destaining. The protein band intensity 

is enhanced by simply rinsing with water. 

• Has the sensitivity to detect as little as 4-8 ng BSA. Staining shows a 

linear response for densitometric gel analysis and produces sharp 

scanning or photographic results. 

• Avoids the use of methanol, acetic acid, or other toxic agents. 

• Works with native PAGE, SDS-PAGE, Iso electric focusing, or 2D gels. 

RUBEO 

• Suitable for high sensitivity 2D 

#462153 200ml $ 58.00 

gel fluorescent staining. 

• Fluorescent: Red emission at #462154 500ml $ 98.00 

605nm. 

• High Sensitivity: Easily detect ng levels of protein. 

• Minimal protein protein variation: Allows quantitative comparison of 

proteins. 

• Compatible: Ideal for subsequent 

analysis, including Mass 

spectroscopy, microsequencing 

and immunostaining. 

• Versatile: Stain native, denaturing, 

IEF and 2D gels. 

• Suitability: Visualize with the 

simplest UV transilluminators to the 

complex laser scanners. 

FASTsilver 

• Detects proteins and nucleic 

#742900 25 mini gels $ 129.50 

acids fractionated by PAGE, 

FASTSilver is a unique 

formulation designed to leave the background exceptionally clear and 

produce sharp images of protein and nucleic acid bands. 

• 100 times more sensitive than Coomassie Blue and 10 times more 

sensitive than Eithidium bromide, FASTSilver detects as little as 1ng 

protein or nucleic acids in 60 minutes. 

• The kit contains regents for 25 mini gels and comes with an easy to 

follow protocol. 

• Kit components: 125 ml Silver Stain, 75g Developer (mixed powder), 4ml 

Sensitizer-I, 4ml Sensitizer-II. 

1000ng 500ng 250ng 125ng 62ng 31ng 15ng 8ng 

SilverOUT Destainer 

• For destaining & removal of 

#515948 100 preps $ 77.50 

silver ions. 

• Used with In-Gel Protein Digestion Kit (catalog #515946) 

Enhanced Silver 

• Silver staining kit with increased 

sensitivity. 

• One kit stains 25 mini gels 


Silver solution 

Sensitizer 

Developer 

Stop solution 

Destainer A 

Destainer B 

#506189 1 Kit $ 149.00 

proteomics Electrophoresis 

New 

49

proteomics 

Protein Stains 

• Mass Silver is a stain that is 

compatable with Mass 

Spectrometry. 

#742750 20 preps $ 149.00 

• Our glutaraldehyde-free formulation #742750 provides the 20 runs sub-nanogram $ 129.95 

sensitivity that you would expect from a silver stain while allowing for 

trypsin digestion required for mass spectrometry procedures. 

New 

New 

MASS Silver 

Crack Free Gel Drying Kit 

• A unique system of cellophane #409506 1 Kit $ 86.90 

sheets and solution regulate 

the water release from the gel to ensure a crack free gel of up to 

20% polyacrylamide. 

• Gel drying is useful for fluorography, densitometry, autoradiography, 

and storage. 

• Contents will dry 20 mini gels. 

• Gel can be native or denatured. 

• Kit includes solution, cellophane sheets 

and drying rack. 

femtoLucent (Chemiluminescent detection 

system for Western Blots) 

Detection System 

2ml Goat anti-mouse lgG 

• Based on an innovative formulation of isoluminol, supersensitive 

detection and washing buffers and AB-horseradish peroxidase (HRP) 

conjugates. The femtoLucent immunodetection system is able to 

detect extremely low concentrations of antigens. 

• Supplied with a novel blocking agent. BLOT-QuickBlocker, which 

allows a rapid blocking step and produces a clear background. 

• Includes detection reagents, detection and washing buffers, BLOT- 

QuickBlocker to perform detection on 5,000cm membrane. 

• A choice of conjugates and other reagents give users the option 

to customize the system to precisely match their Western blotting 

requirements. 

#542400 1 Kit $ 142.00 

#542401 1 Kit $ 95.00 

2ml Goat anti-rabbit lgG #542402 1 Kit $ 95.00 

Yariv’s Reagent 

New 

• Stain binds specifically to 

arabinogalactans in plant and 

microbial cells. 

• Can astain gels and cells. 

#506187 100ml $ 47.00 

50 

> 

accelerate: proteinvisualization 

Glycoprotein Staining Kit 

• Incorporates the proven Schiff’s 

#506186 1 Kit $ 127.00 

reagent and method. 

• One kit contains enough reagents to stain 25 mini gels 


Oxidation Reagent 

Reduction Reagent 

Schiff’s Reagent 

Glycoprotein Standard

BioTRAP (Total Removal of Abundant Proteins) 

Our immunoaffinity columns will remove many of the abundant proteins 

in serum that interfere with proteomics analysis. These include 

albumin, transferrin, haptoglobin, alpha-1-antitrypsin, and many of the 

immunoglobins. By removing these proteins, the remaining serum can be 

concentrated, making detection of low abundance proteins easier. These 

columns are available packed with Sepharose. We also provide columns 

for the removal of any one of these proteins, or a custom mixture of 

depletion supports. Kit includes serum diluent, binding/wash buffer, and 

column regeneration solution. 

Gravity/spin columns with 0.5ml Sepharose-bound Abs 

• bioTrap complete 

• anti-albumin 

• anti-transferrin 

• anti-haptoglobin 

• alpha-1-trypsin 

• Protein A 

#515917 

#515918 

#515919 

#515920 

#515921 

#515922 

1 Kit 

1 Kit 

1 Kit 

1 Kit 

1 Kit 

1 Kit 

$ 247.00 

$ 247.00 

$ 247.00 

$ 247.00 

$ 247.00 

$ 225.00 

bioTrap Multiple Affinity Removal System is comprised of an individual 

peak column packed with polymeric support for HPLC instruments to remove 

six interfering high-abundant proteins (albumin, IgG, IgA, transferin, 

haptoglbin, and antitrypsin) from human serum. 

HPLC Columns 1.6ml PEAK Columns with fitings 

• bioTrap complete 

#515901 1 Kit $ 695.00 

• anti-albumin 

#515902 1 Kit $ 495.00 

• anti-transferrin #515903 1 Kit $ 495.00 

• anti-haptoglobin #515904 1 Kit $ 495.00 

• alpha-1-trypsin #515905 1 Kit $ 495.00 

• Protein A 

#515906 1 Kit $ 385.00 

Single Tissue Protein Blots 

Ready to screen protein blots from different speices 

Description cat. # well price 

Murine Brain 

Murine Kidney 

Murine Ovary 

Murine Heart 

Murine Lung 

Murine Pancreas 

Murine Spleen 

Human Pancreas 

Human Spleen 

Human Testis 

Human Ovary 

Rat Brain 

Rat Kidney 

Multiple Tissue Protein Blots 

#542201 2 blots $ 198.00 

#542202 2 blots $ 198.00 

#542203 2 blots $ 225.00 

#542204 2 blots $ 198.00 

#542205 2 blots $ 198.00 

#542206 2 blots $ 198.00 

#542207 2 blots $ 198.00 

#542226 2 blots $ 198.00 

#542227 2 blots $ 198.00 

#542228 2 blots $ 198.00 

#542229 2 blots $ 24500 

#542230 2 blots $ 198.00 

#542231 2 blots $ 198.00 

Choice of liver, lung, kidney, testies, ovary, pancrease, heart 

and spleen 

Description cat. # well price 

Human 

Mouse 

Rat 

#542250 2 blots $ 523.25 

#542252 2 blots $ 468.65 

#542254 2 blots $468.65 

proteomics Electrophoresis Tools 

New 

proteinpurificationmadeeasy 

51 

for all your electrophoresis supplies visit: www.bio-world.com

iokits&systems 

3 

Analytical 

Cell Biology 

Genomics 

Immunology 

> 

accelerate: cellbiologyresearch 

50

kits Analytical 

Chloride Analysis Test Kit 

Acidity Test Kit 

• For simple & economical 

#061038 1 Kit $ 37.96 

analysis-supplied ready to use. 

• Comes complete with a hard carrying case that helps keep your kit 

organized & protected with a comprehensive instruction manual to 

guide you through the analyses step-by-step. 

• Range & Smallest Increment: 0 to 100mg/L CaCO 3 & 1mg/L 

0 to 500mg/L CaCO 3 & 5mg/L 

• Method: Methyl-orange/Phenolphthalein Titration 

• Applications: 110 

Alkalinity Test Kit 

• Comes complete with a hard 

#061040 1 Kit $ 37.96 

carrying case that helps keep 

your kit organized & protected with a comprehensive instruction 

manual to guide you through the analyses step-by-step. 

• Range & Smallest Increment: 0 to 100mg/L CaCO 3 & 1mg/L 

0 to 300mg/L CaCO 3 & 3mg/L 

• Method: Phenolphthalein/Bromophenol blue Titration 



#061041 1 Kit $ 34.80 





• Range & Smallest Increment: 0 to 100mg/L Cl & 1mg/L 

0 to 1000mg/L Cl & 10mg/L 

• Method: Mercuric Nitrate Titration 


Dissolved Oxygen Test Kit 


#061044 1 Kit $ 59.16 





• Range & Smallest Increment: 0 to 10mg/L & 0.1mg/L 

0 to 5mg/L & 0.005mg/L 

• Method: Modified Winkler Titration 


Formaldehyde Determination Test Kit 

biokits Analytical Tests 

Calcium Assays 

• Sensitive and accurate. Use as 

#061039 1 Kit $ 198.00 

little as 5 mL samples. Linear 

detection range 0.08 mg/dL (20mM) to 20 mg/dL (5mM) Ca2+ in 96-well 

plate assay. 

• Simple and high-throughput. The procedure involves addition of a 

single working reagent and incubation for 3 min. Can be readily 

automated as a high-throughput assay for thousands of samples 

per day. 






• Range & Smallest Increment: 0 to 1% CH 2 O & 0.1% 

0 to 10% CH 2 O & 1% 

• Method: Sulfite/Hyperchloric Acid Titration 


#061076 1 Kit $ 58.00 

> 

accelerate: analyticaltests 

53

iokits 

Analytical Test 

Free Chlorine Determination Test Kit 


#061074 1 Kit $ 39.00 





• Range: 0 to 2.5mg/L Chlorine, Increment 0.5mg/L 

• Method: DPD Colorimetric 


Iodine Test Kit 


#061080 1 Kit $ 39.00 





• Range: 0 to 2.5mg/L I 2 & Increment 0.5mg/L 



Phosphate Assay Kit 

Aminoantipyrine Based Assay 

• Range: 0 to 5mg/L PO 3- 4 & Increment 1mg/L 


• Method: Colorimetric assay 

Malachite Green Based Assay 

#061084 1 Kit $ 35.00 

• Range: 2 to 20µM 

• Measured spectrophotometrically at 620nm, suitable for microtiter plate 

assay. 

Salinity Test Kit 

#061085 1 Kit $ 87.00 


#061068 1 Kit $ 53.36 





• Range: 0 to 40ppt(g/kg) & Increment 0.4g/kg 

• Method: Mercuric Nitrate Titration 


54 

Iron Chemical Test Kit 


#061082 1 Kit $ 39.00 

analysis ready to use. 




• Range: 0 to 5mg/L Fe 2+ , Fe 3 + 

. 



Nitrite (NO) Assay Kit 

• For the quanitification of total 

#061090 1 kit $ 98.70 

NO (a very unstable free radical 

intermediate), a simple assay method is used. 

• The principle involves the metabolic production of nitrite and nitrate 

via NO from L-arginine by inducible or noninducible NOS. 

• The nitrite formed reacts with sulfanilamide and N-(1-naphthyl)- 

ethylinediamine dihydrochloride to give a red-violet diazo dye, 

measured by its absorbance at 540 nm. 

• Assay can be completed in less that 30 minutes. 

• Applications: 

Signal transduction 

Immune response 

Inflammation 

New 


Sulfite Test Kit 


#061086 1 Kit $ 66.12 





• Range & Smallest Increment: 0 to 20mg/L Na 2 SO 3 & 0.2mg/L 

0 to 200mg/L Na 2 SO 3 & 2mg/L 

• Method: Iodometric Titration 


Caspase Apoptosis Assay Kit 

• Provides reagents for the detection of apoptosis by measuring the 

caspase activity - an early indicator of apoptosis. 

• Assay can be monitored with a fluorescence reader, a 

spectrophotometer or microtiter plate reader. 

• Uses AFC-substrate 

• If the assay is measured with a spectrophotometer or microtiter plate 

reader, the absorbance is measured at 390-400nm. 

• Can be conveniently adapted for high-throughput 96-well format. 

• System may be used with purified enzyme preparations, cell extracts or 

kits Cell Biology 

tissue lysates. 

• Supplied with necessary assay buffers, enzyme specific AFC-substrate, 

free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper 

positive and negative controls and standards. 

• Permits in situ PCR reactions. 

A nonhazardous tissue 

Caspase 1,4,5 

#526110 50 assay $ 149.00 

In-situ® Fixative 

New 

#710150 500ml $ 37.00 

fixative exempt from EPA disposal regulations. Virtually odorless 

Inactivates the HIV-1 virus in culture 

#526112 100 assay $ 198.00 

• Maintains cellular morphology 

Optimizes immunohistochemistry results 

Caspase 2 

#526114 50 assay $ 149.00 

#526116 100 assay $ 198.00 

biokits 

Cell Biology 

Quick de WAX (xylene substitute) 

New 

• A non xylene based paraffin removal media for quick 

#506122 1L $ 48.20 

dewaxing of sections. Ideal for insitu PCR, hybridizations and 

immunoreactions. 

Peroxidase (in situ) Removal Kit 

• To remove endogenous 

#506155 1 Kit $ 49.20 

peroxidase, treat the samples 

before primary antibody reaction with the reagent provided in this kit. 

• 1 assay 

Caspase 3,7,10 

Caspase 6 

Caspase 8 

Caspase 9 

#526118 50 assay $ 151.15 

#526120 100 assay $ 199.70 

#526122 50 assay $ 149.00 

#526124 100 assay $ 199.70 

#526126 50 assay $ 149.00 

#526128 100 assay $ 199.70 

#526130 50 assay $ 149.00 

#526132 100 assay $ 199.70 



F: 614.792.8685 


55 56

iokits 

Genomics 

Protoplast Isolation Kit 

New 

• For isolation of protoplasts 

#760512 1 Kit $ 167.00 

from plant tissue. 

• Contains: All necessary enzymes, buffers & reagents. 

• Other materials NOT provided: 4-6 day old plant culture, sterile 50ml 

centrifuge tubes, centrifuge with swing-out 

rotor, sterile 250ml flask, platform shaker, 

hemacytometer & microscope. 

kits Gene 

metaDNA 

• A kit to isolate total DNA from 

#525158 1 Kit $ 98.00 

soil samples capable of 

generating PCR products free of any taq-polymerase inhibitor. 

• Sufficient reagents for 50 samples. 

PlantGene 

Plant Genomic DNA Extraction Kit 

• PlantGene Plant Genomic 

DNA extraction kit provides a 

rapid protocol for extracting plant genomic DNA without 

contamination of polysaccharides and any organic extractions. 

• An average yield of 20-100µg DNA/gram tissues obtained. 

• The average size of DNA is usually more than 20Kb and in highly 

purified form, ready for restriction enzyme digestion and cloning. 

• Kit includes all necessary reagents and protocols. 

Apoptotic DNA-Ladder 

• For the production of Apoptosis 

Nucleosomal DNA Ladders. 

• The Apoptotic DNA-ladder 

#525161 50 preps $ 115.00 

kit contains all of the reagents necessary for the preparation of an 

apoptotic DNA ladder. 

• The protocol involves cell lysis, removal of cellular debris, followed 

by precipitation of DNA. 

• Nucleosomal DNA is detected after standard 1.8% agarose gel 

electrophoresis. 

#525105 100 tests $ 130.00 

DNA-pure Yeast Genomic 

• Easy 30-minute protocol 

• High yield of quality, intact 

yeast genomic DNA 

#525175 50 preps $ 115.00 

• No lytic enzymes or proteinase K needed 

• No organic extractions 

• DNA can be used directly for restriction enzyme, digestion, PCR and 

hybridization. 

56 


ArrayHyb Kit 

• Contains DNA hybridization solution for microarray analysis. 

• Sufficient product for 50 microarray hybridizations. 

With Ficoll #515932 1 Kit $ 64.20 

With Formamide #515931 1 Kit $ 59.60 

Without Formamide #515930 1 Kit $ 48.70 

Gene Bullet 

• Diameter: 0.8-1.5 microns 

• Offered in Tungsten (T) & Gold (G) particles; provides the most suitable 

carrier of foreign genes using particle bombardment. 

• Coat the DNA/RNA with the Gene Bullet & you are ready for delivery 

into host. 

• Suitable for animal & plant cells/tissues. 

• Suspension is sterile & contains 50% Glycerol. 

• Store at -20˚C to prevent oxidation. 

biokits 

Z-Competent Transformation Kit 

• Simple, highly efficient method for preparing cells. 

• No heat shock or any other manipulation is required. 

• Routinely produces 2x10 8 to 1x10 9 transformants per 1µg supercoiled 

pUC19 DNA. 

Basic 

#525355 300 preps $ 91.50 

Gold - Powder 

Gold - Ready To Use 

Tungsten - Powder 

#520122 100mg $ 87.50 

#520121 250mg $ 147.50 

#520120 2x0.5ml $ 59.50 

#520110 5G $ 145.00 

Genomics 

+ SOB Medium 

#525357 300 preps $ 135.95 

Tungsten - Ready To Use 

#520111 2 x 0.5ml $ 59.50 

#520112 2 x 1ml $ 79.70 

Fasttrack Yeast Transformation Kit 

• Simple protocol for multiple 

plasmid transformation. 

#512950 120 preps $ 114.95 

• High transformation efficiency, yields approximately 10 5 -10 6 

transformants per microgram circular DNA. 

• Broad spectrum kit works well with C. albicans, S. pombe, P. 

pastoris and S. cerevisiae. 

• Transformation procedure takes less than an hour. 

• Frozen competent cells are good for use up to a year. 

• Rapid single step yeast transformation kit takes less than 10 minutes to 

prepare competent cells. 

• Protocol involves simply suspending yeast cells in the “Competent 

Buffer” for immediate use. 

• Cells are ready to receive transforming DNA. 

• Grow cells by applying appropriate selection for cloning your 

desired sequence. 

GeneExit 

• Uses a superior proprietary binding matrix PinkResin. 

• Non toxic system provides you with DNA ready for any 

downstream application. 

• Suitable for extraction of DNA fragments from 100bp to more than 

20kb from both TAE & TBE buffer and gels. 

• One kit will perfom 100 preps. 

DNA Isolation Kit 

#515983 1 Kit $ 50.40 

Isolation Kit w/ Column #515984 1 Kit $ 129.65 

57 56

iokits 

Genomics 

Gene Capsule 

• Single use device for rapid recovery of DNA, RNA & protein from gels, 

agarose or acrylamide. 

• No spinning, washing, heating, precipitation or damage to 

your sample. 

• Uses the principle of electroelution & requires no additional equipment. 

• Procedure takes as little as 90 seconds to recover 1000bp DNA. 

• Has two parts, Gel-Pick & Gene-Trap. 

• Users simply pick up the DNA, RNA or protein band with the Gel-Pick & 

assemble with the Gene-Trap. 

• Rapid electroelution & DNA, RNA or protein is recovered in 25-40µl. 

• Recovery is as high as 90%. 

• Can be used for extraction of DNA, RNA & protein as small as 20bp 

or a MW of 4000. 

• Recovered DNA is ready for ligation, restriction enzyme digestion, 

sequencing, amplification, random priming, nick translation or other 

enzymatic reactions. 

Accessory Kit #515703 1 Kit $ 29.15 

• includes small weight device and forceps 

Large (6-8mm wide) 

#515701 1 Kit $ 148.65 

• 54 assays 

BioFectin (Transfection Reagent) 

• BioFectin Lipid Reagent #515555 0.5ml $ 105.00 

provides high efficiencies and 

#515556 1.0ml $ 195.00 

transgene activity in a broad 

range of commonly used cell lines, while lowering the cytotoxic effects 

observed with other high-efficiency transfection reagents. BioFectin 

is composed of a proprietary cationic lipid and co-lipid (1,2-dioleoylsn-Glycero-3-Phosphoethanolamine) 

and works well when working with 

difficult-to-transfect and sensitive cell lines. The BioFectin protocol 

allows for a high degree of flexibility in your culture conditions, and 

provides a simple three-step method that can be easily adapted to highthroughput 

applications. 

New 

Hybridization Cocktail 

• Designed to optimize complementary base pairing of DNA & RNA in 

Southern Blot, Northern Blot, Dot Blot, Colony & in situ hybridizations. 

• Components of these cocktails stabilize the target & probe nucleic 

acids, lower melting temperatures, concentrate the nucleic acids in 

solution & lower background on the membrane or slides. 

• Storage: 2-8˚C 

50% Formamide 

#705475 50ml $ 38.35 

58 

Electroporation Cuvettes 

• For electroporation of DNA, RNA and protein 

in bacteria, yeast, animal, and plant cells. 

• New and improved electrodes. 

• Fits most major brands of electroporators. 

• Individually packaged and sterilized by 

UV/gamma irradiation. 

1mm Gap #039506 50/pk $ 125.00 

2mm Gap #039507 50/pk $ 125.00 

4mm Gap 

#705473 

#039508 

50ml 

50/pk 26.30 

$ 125.00 

#705477 100ml $ 50.65 

No Formamide 

Nucleic Acid Stripping Buffer (10X) 

- for hybridization of probes to nucleic acids 

Nitrocellulose 

• SSC (0.5X) 

• EDTA (100mM) 

Nylon Membrane 

• Tris HCl 

• pH 8.0 (1mM) 


• Denhardt’s solution 1X 

#705479 100ml $ 55.07 

#705477 100ml $ 50.65 

#740125 250ml $ 26.75 

#740126 500ml $ 49.60 

#740127 250ml $ 29.50 

#740128 500ml $ 54.50 


Nucleic Dotmetric Kit 

Phase-Guard 

Basic 

#515570 1 Kit $ 166.15 

Tips & Board 

#515572 tips/board $ 249.25 

• 1µL assay for DNA, RNA & Oligonucleotide quantitation in 3 minutes. 

• Measures spot diameter, not intensity, to accurately determine 

concentration of DNA, RNA or oligonucleotides in minutes. 

• Sensitive enough to detect as little as 1-5 ng/µL of nucleic acids. 

• Based on the direct relationship between nucleic acid concentration & 

the diameter of the circular nucleic acid spot produced on the test strip. 

• Determine DNA, RNA or oligo concentration by measuring the 

diameter of the nucleic acid spot using the Nucleic dotMetric gauge 

supplied with each kit. 

• 300 Assays/Kit 

• Economical alternative to spectrophotometry - no expensive equipment 

or cuvettes are necessary. 

• Available with a Spot Application Board (magnetic) for securing test 

strips and dotMetric compatible sample application tips (Cat# 515572). 

• Indispensable for protocols with organic extraction of DNA & RNA. 

• Increase nucleic acid recoveries by up to 30%. 

• Eliminate contamination with interface material. 

• Single tube enzymatic reactions & extractions. 

• Pre-dispensed for convenience into standard lab centrifuge tubes. 

• Reduces exposure to hazardous organic solvents. 

• Inert, heat stable proprietary gel, present during organic extractions 

migrates under centrifugal force to form a seal between the organic & 

aqueous phases. 

• The organic phase & the interface material are effectively trapped 

below the seal. 

• Barrier allows complete, easy transfer of the entire aqueous phase, by 

simply decanting or pipetting to a fresh tube. 

• Comes in two density formulations, Heavy & Light and can be adapted 

to virtually any protocol requiring extraction with phenol and/or 

chloroform by selecting the formulation that is compatible with the 

organic phase used. 

• Does not interfere with standard nucleic acid restriction & 

modification enzymes. 

• Can be present in reactions requiring a heat inactivation step 

(65˚C for 10 minutes). 

biokits 

Genomics 

Light Density #540608 1-6ml $ 70.20 

(50 tubes) 

#540609 1-6ml $ 124.80 

(100 tubes) 

#540600 10-35ml $ 84.50 

(50 tubes) 

Heavy Density 

#540610 1-6ml $ 80.20 

(50 tubes) 

#540603 3ml syringe $ 84.50 

(10 tubes) 

#540604 20-100ml $ 84.50 

(100 tubes) 



F: 614.792.8685 


> 

accelerate:genomicsresearch 

59 56

iokits 

Genomics 

Plasmid Purification Powerprep Kit 

• High quality plasmid purification based on alkaline lysis. 

• Plasmid is allowed to bind to PinkResin, a binding matrix, followed by 

quick washing steps & elution of plasmids using spin columns. 

• Suitable for restriction enzyme analysis, fluorescent & radioactive 

sequencing, subcloning, transfection & in vitro transcription. 

• Mini yields from 1.5ml culture, Large from 50-100ml culture. 

Mini 

#515985 50 preps $ 82.75 

#515986 100 preps $ 148.25 

Large #515987 10 preps $ 105.80 

#515988 20 preps $ 199.25 

Rapid RNA Purification Kit 

• Biotechnology Grade 

• Total RNA 

#705881 1 Kit $ 107.10 

• Reagents included in kit: Reagent 1, Tissue Homogenization 

Suspension, Reagent 2, Reagent 3, Reagent 4, RNA Precipitation 

Reagent & Homogenization Pestles. 


RNA-Total Arrest 

• Optimized mixture of 

#535101 10 preps $ 37.40 

chaotropic agents & RNase 

inhibitors which eliminates #535100 50 preps $ 129.95 

RNase activity in 5 seconds & is universally compatible with phenol, 

chloroform & other detergent-based RNA purification protocols. 

• Contains enough reagent to isolate RNA from 10 grams of tissue or 200 

preps of 50mg tissue each. 

• Permits extremely rapid isolation of total RNA from cells, regardless of 

origin & is free of DNA & protein. 

Southern and Northern blots Hybridization 

Saline Sodium Citrate (20X) 

• Ultra Pure Grade 

#730296 2x1L/ready pk $ 27.57 

• Chloride content (1% water) 

(mg/L) 110-120 

#730298 1L lq concentrate $ 27.50 

• pH (1% water) at 25°C: 7.85- 

8.15 

• Hazard: Irritant 


Prehybridization Solution 

• SSC 6X 

• Denhartdt’s solution 5X 

• SDS (0.5%) 

• Denatured, phenol-chloroform 

extracted from salmon DNA (100µg/ml) 

Hybridization Solution 

• SSC 6X 

• Blotto (5%) 

BIOzol Plant RNA Isolation Kit 

New 

#730299 2L lq concentrate $ 49.70 

- hybridization of probes to nucleic acids 

#700830 50ml $ 46.20 

#700831 100ml $ 89.20 

- hybridization of probes to nucleic acids 

#700834 100ml $ 34.50 

• BIOzol Kit is a single phase 

#527700 1 Kit $ 98.50 

solution designed specifically 

for the isolation of pure, intact RNA from tissue and cells in less than 

90 minutes. 

• Kit utilizes guanidine thiocyanate and ß-mercaptoethanol, two of the 

most potent inhibitors of RNase, to effectively disrupt cellular 

structures while inactivating RNase released from membrane-bound 

organelles. 

• Total RNA of free protein, and DNA and can be used for cDNA synthesis, 

Northern blotting and in vitro translation. 

• Procedure eliminates the need for lengthy ethanol precipitations and 

cesium chloride gradients. 

• One kit performs 100 RNA isolations (each from 100 milligrams of 

tissue) Product can be used as a cost effective substitute to TRIzol 

60 


SSPE Buffer - for use in nucleic acid hybridization applications 

DNA Supperladder Molecular Markers 

20X Solution 

• Each liter prepares 20L of 1X 

Saline-Sodium-Phosphate- 

EDTA buffer 


• pH at 25°C: 7.25-7.55 



Hybridization Solution 

• SSPE 6X 

• Denhartdt’s solution 5X 

• SDS (0.5%) 

• Denatured, phenol-chloroform 

extracted salmon testes DNA (100µg/ml) 

Prehybridization Solution 

#730124 4L concentrate $ 49.14 

#730123 2x1L/ready pk $ 24.57 

#730126 1L solution $ 25.80 

#705917 4L solution $ 58.80 

#700832 50ml $ 43.50 

#700833 100ml $ 76.50 

• SSPE 6X 

• Blotto 5% 

#700836 100ml $ 34.50 

#700837 200ml $ 68.50 

DNA Superladders 

High Mid-1 Mid-2 High 

Low Ladder 

• Covers an optimal range for the 

definitive sizing of PCR & 

restriction generated 

DNA fragments. 

• The 20bp ladder brings 

unprecedented accuracy (1-2%) 

to routine agarose or 

polyacrylamide gel sizing 

of DNA. 

• The 100bp ladder consists of 10 sharp bands with very low background. 

• Excellent quality & a better value per microgram than any ‘ready-to- 

load’ ladders available. 

• Provide 2-in-1 ladders; combined ladders yield bright-easy-to read main 

divisions & lighter subdivisions from 20bp to 100kb. 

• No overload plasmid DNA bands. 

High Ladder 

• Designed for pulsed field or 

very low percentage agarose 

gel electrophoresis. 

• Provides accurate sizing of 

large DNA fragments. 

• The 2.5kb is prepared from 

concatamers of a highly 

purified DNA subunit with 

precisely 50% G-C content. It has a brighter band at 10kb. 

• The 5kb is made of concatamers of pBR322-derived vector 

DNA sequences. 

• Provide 2-in-1 ladders; combined ladders yield bright, easy to read main 

divisions & lighter subdivisions from 20bp to 100kb. 

• No overload plasmid DNA bands. 

#745102 40UG $ 128.25 

(20bp) 

#745202 150UG $ 370.50 

(20bp) 

#745101 40UG $ 114.75 

(100bp) 

#745201 150UG $ 331.50 

(100bp) 

#745132 40UG $ 148.50 

(2.5kb) 

#745232 50UG $ 168.50 

(2.5kb) 

#745135 40UG $ 128.25 

(5kb) 

#745235 50UG $ 138.25 

(5kb) 

biokits 

Genomics 

Low Marker Kit 

• (For info see Low Ladder). 

• Kit includes separate tubes of 

#745100 Kit $ 128.25 

20bp ladder (25µg, 50-75 wells) & 100bp ladder (25µg, 100-150 wells), a 

pre-labeled tube for storing the ladder mixture, 6X loading buffer 

& instructions. 

61 56

DNA Supperladder contd. 

biokits 

Genomics 

High Marker Kit 

• (For info see High Ladder) 

#745230 25UG $ 141.75 

• Kit includes separate tubes of 

5kp ladder (75µg) & 2kb ladder (75µg), 6X loading buffer 

& instructions. 

Mid1 Marker Kit 

• Range: 100bp to >15kb 

#745210 150UG $ 375.50 

• A 500bp ladder & a 100bp 

ladder extending to 4kb. 

• Kit includes separate tubes of 100bp ladder (75µg) & 500bp ladder 

(150µg), a pre labeled tube for storing the ladder mixture, 6X loading 

buffer & instructions. 

Mid2 Marker Kit 

• Consists of a novel 200bp 

#745220 150UG $ 429.00 

ladder providing excellent 

resolution up to 8Kb & the highest range 1Kb ladder available, 

extending above 15kb. 

• Brighter band at 5kb. 

• Kit includes separate tubes of 1Kb ladder (150µg) & 200bp ladder 

(150µg), a pre labeled tube for storing the ladder mixture, 6X loading 

buffer & instructions. 

Genomic DNA Extraction System 

This kit uses a silica-gel membrane for simple and fast isolation 

of genomic DNA without the use of toxic chemicals. Purified DNA 

is free from contaminants and usually has an absorbance ratio 

between 1.7 and 1.9 (A 260 /A 280 ). DNA can be used for such applications 

as: Restriction digestion, Southern Blotting, RAPD, RFLP, 

PCR and Real-time PCR. Preparation time is less than 2 hr. 

Blood and Tissue Genomic DNA 

Miniprep 

• 1 x 10 9 cells of Bacteria/ Yeast 

Yields up to 80µg/DNA 

• 200µl whole blood. Yields up 

to 10µg/DNA. 

• 30mg animal tissue. Yields up 

to 120µg/DNA. 

• 0.1g plant tissue. Yields up to 40µg/DNA. 

Maxiprep 

• 5ml whole blood. Yields up to 

300µg/DNA 

• 1.0g plant tissue. Yields up to 

1.0mg/DNA 

Sample Amount Yield 

Whole Blood 

Animal Cells 

Animal Tissues 

Mouse Tail 

Bacteria/Yeast 

Parraffin- 

Embedded Tissue 

200µl 

10 7 cells 

30mg 

0.5cm 

1x10 9 /10 8 cells 

25mg 

up to 10µg 

up to 120µg 

up to 120µg 

up to 15µg 

up to 80µg 

up to 120µg 

#078507 50 preps $ 39.50 

#078508 250 preps $ 147.00 

#078509 10 preps $ 59.00 

#078510 50 preps $ 187.00 

62 


Pink DNA Cleanup Kit 

• For cleaning PCR products, 

purifying DNA fragments after 

in vitro labeling reactions, restriction enzyme removal from plasmid 

DNA prior to in vitro transcription or cleaning any DNA preps. 

• Removes salts, enzymes, unicorporated nucleotides, radiolabels after 

P 32 or S 35 labeling & primer-dimers from any DNA prep of 100bp to 

more than 20kb. 

• 200 preps 

Glass Milk 

#515980 1 Kit $ 75.50 

Midi-V100 

Viagen Ultrapure Plasmid Extraction System 

• Designed for rapid isolation of ultrapure plasmid DNA from a range 

of 25-500ml Bacterial Culture. Uses Gravity-flow anion-exchange tips 

for efficient purification of plasmid DNA. 100-500µg of ultrapure 

plasmid DNA can be isolated without using toxic chemicals. 

Features: 

• Rapid and simple extraction 

• Reproducible results 

• Non-toxic reagents 

Plasmid Culture Volume Elution Volume Yields 

High copy number 25-50ml 100µl up to 100µg 

Low copy number 50-100ml 100µl up to 100µg 

biokits 

• Specially prepared proprietary 

suspension of propriety silica 

matrix in an aqueous solution 

used to isolate & purify DNA. 

• Provides rapid, quantitative, specific binding of DNA/RNA & is able to 

clean DNA from solution or clear gel bands. 

• DNA purified by Glass Milk shows no effect on sequencing reactions, 

restriction digests, ligations or phosphorylations. 

• Also suitable for mRNA preparation. 

#700101 5ml $ 49.50 

#700102 20ml $ 116.18 

A proven low cost tool for 

DNA/RNA isolation! 

Maxi-V500 

High copy number 100-250ml 300µl up to 500µg 

Low copy number 350-500ml 300µl up to 500µg 

Midi-V100 

• 25 preps: 

25 Midi-V100 Columns, 50 

#078526 25 preps $ 95.00 

Mini-M Columns, RNase A, #078527 50 preps $ 169.00 

Buffers 

• 50 preps: 

50 Midi-V100 Columns, 100 Mini-M Columns, RNase A, 

Buffers 

Genomics 

Yeast-Genome-DNA-Template Extraction 

• Based on a two-step lysis of 

#526107 1 Kit $ 159.00 

yeast cells & removal of protein 

& other cellular impurities by centrifugation. 

• DNA extracted is a high yield & on average is 100Kb in size with an 

A260/280 1.8-2.0. 

• Supplied with reagents for extracting 100 samples from 1.5ml cultures. 

Maxi-V500 

• 10 preps: 

10 Maxi-V100 Columns, 20 

#078528 10 preps $ 145.00 

Mini-M Columns, RNase A, 

Buffers 

• 25 preps: 

#078529 25 preps $ 215.00 

25 Maxi-V100 Columns, 50 Mini-M Columns, RNase A, 

Buffers 



F: 614.792.8685 


> 

accelerate:genomicsresearch 

63 56

iokits 

Genomics 

ColonyPCR Kit 

• The Colony PCR kits contains all 

#522705 1 Kit $ 58.70 

the reagents necessary to 

rapidly extract genomic DNA from a single colony and amplify targets of 

interest by PCR. 

• Sufficient reagents for 50 reactions. 

• Kit includes protocol, sample extraction reagents and components for 

PCR reaction such as Taq polymerase and dNTPs.. 

BonePCR Kit 

• Quick sample extraction kit for 

#522702 100 assays $ 97.50 

direct bone PCR from bone 

material. 


• Extracted sample can be stored at 4ºC for several weeks. 

• Kit includes protocol and the sample extraction reagents. 

• Includes the components for PCR reaction such as polymerase FirePol and 

dNTPs and reaction buffer. 

BloodPCR Kit 

• Contains all the reagents 

#522703 1 Kit $ 69.00 

necessary to rapidly extract 

genomic DNA from whole blood and amplify targets of interest by PCR. 


• Kit includes protocol, sample extraction reagents and components for 

PCR reaction such as Taq polymerase and dNTPs.. 

LeafPCR Kit 

• Quick sample extraction kit for 

#522701 1 Kit $ 67.50 

direct PCR from leaf 

material. 

• Sufficient reagents for 50 samples. 

• Extracted sample can be stored at 4ºC for several weeks. 

• Kit includes protocol and the sample extraction reagents. 

• Includes the components for PCR reaction such as Taq polymerase 

and dNTPs. 

PCR 

FirePol and Hot FirePol 

FirePol 

#516222 1000u $ 79.00 

#516227 4000u $ 299.00 

Hot FirePol 

#516220 1000u $ 99.00 

• A thermostable DNA polymerase 

• FirePol is similar to Taq DNA 

polymerase (homology 99%) 

• 3’ to 5’ exonuclease activity 

#516226 4000u $ 386.00 

• Has the “extendase activity”, allowing TA cloning 

• Due to the unique modifications and purification technology it is very 

thermostable at high temperatures. 

• Can be used in all applications involving polymerase chain reaction (PCR). 

• Contains necessary reaction buffers and MgCl 2 . 

64

kits Immunology 

Antigen-Antibody Dissociation Kit 

• Acidic dissociator pH 2.5, 50ml; 

neutralizer pH 8.5, 10ml. 

• Two reagents are provided to dissociate your antigen-antibody complex 

from a column. 

#650161 50ml $ 27.40 

• Dialyze to desalt after dissociating with neutral dissociator. 

• Neutralize your antibody after elution with acid dissociator. 

Unmask Kit 

• An antigen retrieval system in 

fixed sections. 

• Provides a set of reagents that individually or in combination, allow 

maximum retrieval of any antigen for antibody reaction to maximize 

immunohistology results 

#506100 1 Kit $ 67.50 

ImmunoMax Kit 

• For maximum antigen-antibody 

#506110 1 Kit $ 74.40 

reactivity in solution, cryofixed 

sections & paraffin embedded 

sections. 

• Single amplification technique, which allows the signal to be enhanced 

significantly above the low background. 

• Up to 200-fold signal amplification achieved by covalent deposition 

of biotin reporter molecules, combined & modified retrieval & 

amplification steps & a new maximized immunohistochemical staining 

protocol (termed ImmunoMax Method). 

• Enhances the sensitivity of the reaction up to 1000-fold. 

• Antigens previously thought to be nonreactive or insufficiently 

detectable in formalin-fixed, paraffin-embedded tissues can now 

be detected. 

• Kit supplied with a detailed protocol, may be ordered with or without 

the antigen retrieval system. (catalog #506100) 

biokits 

Immunology 

New 

An efficient antigen retrieval 

system for immunohistology 

Protein A-Gold 

17-23nm particle size 

#506445 2 ml $ 89.30 




#506448 2 ml $ 89.50 

#506449 500µL $ 29.30 

#506447 500µL $ 29.70 

• Capable of binding to the fc portion of immunoglobulins, especially 

IgG. 

• Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 

1% bovine serum albumin, 20% glycerol and 15 mM sodium azide. 




F: 614.792.8685 


Wearing gloves is highly recommended when handling kit contents 

> 

accelerate:immunohistology 

65 56

uffers&reagents 

4 

DNA / RNA 


General Use 

Hybridization 

Immunologicals 


> 

accelerate: lifeSciencesresearch


DNA / RNA 

DNA Denaturing Solution - guaranteed sterile and 


• 1.5M NaCl, 0.5M NaOH 

• pH (25°C) >11.5 


• Hazard: Corrosive 

Glucose / Tris / EDTA (Sterile) 

• Glucose (50mM) 

• Tris (25mM) 


• pH 7.9 - 8.1 

Plasmid DNA Isolation 

Solution 1 

• Glucose (50mM) 

• Tris-HCl, pH 8.0 (25mM) 

• EDTA, pH 8.0 (10mM) 

Solution 2 

• NaOH (0.2N) 

• SDS (1%) 

Solution 3 

• Potassium (3M) 

• Acetate (5M) 

All 3 Solutions 

DNase-free 

#700845 500ml $ 21.50 

#730601 1L $ 37.50 

#730605 500ml $ 27.51 

#730606 1L $ 39.00 

#700860 250ml $ 21.55 

#700861 500ml $ 34.90 

#700863 250ml $ 23.00 

#700864 500ml $ 34.90 

#700866 250ml $ 19.50 

#700867 500ml $ 34.90 

#700859 250ml $ 59.70 

QBT Buffer - for DNA purification 

• NaCl (750mM), pH 7.0 

• MOPS (50mM) 

• Ethanol (15%) 

• Triton X-100 (0.15%) 

QC Buffer - for DNA purification 

• NaCl (1.0M), pH 7.0 

• MOPS (50mM) 


QF Buffer - for DNA purification 

• NaCl (1.25M), pH 8.5 

• Tris-Cl (50mM) 


Sodium Acetate (3M) 

• Autoclave sterilized 


• pH 5.2 

#700870 250ml $ 24.50 

#700871 500ml $ 39.70 

#700909 250ml $ 22.50 

#700910 500ml $ 37.50 

#700912 250ml $ 22.50 

#700913 500ml $ 37.50 

#730120 100ml $ 16.59 

#730122 200ml $ 22.47 

Sodium Hydroxide with MSH - for protein and DNA 

isolation from yeast 

• NaOH (1.85M) 

• β−Mercaptoethanol (7.5%) 

STE Reagent - for molecular biology 

• NaCl (100mM) 



#700225 250ml $ 21.50 

#700226 500ml $ 38.50 

#700918 1L $ 19.50 

#700919 2L $ 35.00 

buffers&reagents DNA / RNA 

DNase, RNase & Protease-free buffers 

67 




TE (Tris / EDTA) - buffer for DNA isolation & electrophoresis 

• Sterile Solution 


#730153 100ml $ 16.84 


• pH 8.0 ( + - 0.1) 

• DNase : None 


#730155 500ml $ 49.70 

SDS Urea Buffer- - for protein & DNA isolation from yeast 

• SDS (5%) 

• Urea (8M) 

#700227 250ml $ 19.50 

• Tris-HCl, pH 8.0 (50mM) #700228 500ml $ 36.90 

• Bromophenol Blue (0.005%) 

Tris Buffered Saline - for Western blot washing 

High Salt (10X) 

• pH at 25°C 7.4 


#740116 1L $ 23.75 

• NaCl (5M) 

#740117 2L $ 42.75 


#740118 4L $ 66.50 

buffers&reagents 


Agarose Gel Loading Dye 6X - for molecular biology 

Glycerol with Xylene Cyanol FF 

• No Bromophenol Blue 

• Xylene Cyanol FF (0.25%) 

• Glycerol (30%) 

Glycerol with Bromophenol Blue 




Alkaline 

• NaOH (300mM) 


• Bromocresol Green (0.25%) 


• Ficoll (18%) 

Ficoll 



• Ficoll in water (15%) 

#700813 25ml $ 19.00 

#700814 50ml $ 27.50 

#700811 25ml $ 19.70 

#700812 50ml $ 28.50 

#700820 25ml $ 28.50 

#700821 50ml $ 47.50 

#700818 25ml $ 28.50 

#700819 50ml $ 47.50 

Low Salt (10X) 

Sucrose 

• pH at 25°C 7.4 


• NaCl (1.5M) 

#740111 1L $ 19.95 

#740112 2L $ 35.15 



• Sucrose (40% w/v) 

#700815 25ml $ 19.00 

#700817 50ml $ 27.50 


#740113 4L $ 66.50 

68 

Yeast Lysis Solution - for DNA isolation 

• LiCl (2.5M) 

• Tris-HCl (50mM), pH 8.0 

#700223 250ml $ 29.00 

• Triton X-100 (1%) 

#700224 500ml $ 45.60 

New • EDTA (62.5mM) 

Alkaline DNA Gel Running Buffer 

• Biotechnology grade 

#700841 250ml $ 19.50 

• NaOH (0.5M) 


#700842 500ml $ 35.00 

Sterile, nuclease-free buffers & reagents have 

been prepared with the finest Biotechnology 

Grade chemicals in specially purified 3D water 

treated to remove particulates, salts, dissolved trace 

metals, organic compounds & microorganisms.

Orange-G Loading Dye - for molecular biology 

Glycerol (6X) 

• 6X concentration 

• Orange-G (0.25%) 

#700824 25ml $ 19.00 

• Glycerol in water (18%) 

Blue (6X) 

#700825 50ml $ 28.50 

• Orange-G (0.25%) 


#700822 10ml $ 19.00 


• Ficoll in water (30%) 


#700823 25ml $ 34.50 

Isoelectrofocusing (IEF) Buffer 

Anode (50X), pH 2.4 

• Dilute with deionized water 

to make 1X concentration. 

#743140 500ml $ 16.25 

• 1X concentration: PH 2.4 #743141 1L $ 26.75 

Phosphoric Acid (7mM) 

• Proteases free 

Cathode (10X), pH 10.1 



#743142 250ml $ 36.25 

• 1X concentration: 20mM #743143 500ml $ 62.70 

Arginine and 20mM Lysine 

Sample Buffer (4X) - for protein electrophoresis 

IPG-Strip Equilibration Buffer 

• For equilibration of IPG- Strip 

before running second 

dimension SDS-PAGE. 

• No urea-induced protein 

carbamylation 

• No waste of unused reagents 


#705940 50ml $ 49.50 

#705955 100ml $ 86.60 

• Improves resolution and prevents 

streaking of protein spots on 2D gels. 

Laemmli SDS-Sample Buffer - electrophoretic 

dye for denaturation of proteins 

Reducing, (4X - 6X) 

• 1X solution: SDS (2%), Glycerol 

(10%), Tris - HCl (62.5mM), 

Bromophenol Blue and 

β - mercaptoethanol 

• DNase and Protease free 

• Stability (20°C) >1 year 

Non-reducing, (4X - 6X) 


• 1x solution: SDS (2%), Glycerol 

(10%), Tris -HCl (62.5mM), and 

Bromophenol Blue. 

• DNase, RNase and 

Protease free 

• Stability (20°C) >1 year 


4X- #742126 50ml $ 28.50 

6X- #742114 25ml $ 24.50 


4X- #742124 25ml $ 19.00 

#742118 50ml $ 28.50 

6X- #742110 25ml $ 24.50 

#742112 50ml $ 39.80 

buffers&reagents Electrphoresis 

• 20mM Lysine (free base) and 

Glycerol (15%) 

#742133 25ml $ 19.00 

#742134 50ml $ 28.50 

MES-SDS Running Buffer (20X) 

> 

accelerate: electrophoresis 



• 1X concentration: MES 

(50mM), Tris base (50mM), 

SDS (0.1%), EDTA (1mM) 

• pH 7.3 

#743146 500ml $ 34.50 

#743147 1L $ 59.70 

56 

69 




SDS Sample Loading Buffer 

• Standard SDS polyacrylamide 

#700958 50ml $ 29.50 

electrophoresis sample loading 

buffer 

#700957 100ml $ 39.70 

• 0.05% (w/v) bromophenol blue 

• Dilute samples to at least 1:4 

with the sample buffer. 

#700956 200ml $ 67.50 

• Heat samples at 95ºC for 4 minutes prior to loading. 

SDS Separating Gel Buffer (4X) 


gel 10% electrophoresis 

#700952 100ml $ 00.00 

separating gel buffer 

#700951 250ml $ 00.00 

• Buffer in 0.375M Tris, pH 6.8 

• 20% (w/v) SDS 

#700950 500ml $ 00.00 

SDS Stacking Gel Buffer (4X) 


gel 10% electrophoresis 

separating gel buffer 

• Buffer in 0.375M Tris, pH 8.8 

• 20% (w/v) SDS 

#700954 

#700953 

#700950 

100ml 

200ml 

500ml 

$ 00.00 

$ 00.00 

$ 00.00 

TAE Buffer (Tris Acetate EDTA) - for electrophoresis 

Ready-Pack (10X) 

• Each Ready-Pack prepares 

1L of 10X concentrate. 

• pH 8.3 + - 0.1 

Ready-Pack (25X) 

• Each pack makes 1L of 25X 

• 1X contains: Tris (0.04M ), 

Acetate (0.04M ), EDTA 

(0.001M) 

• pH at 25C: 8.2 - 8.4 

• (P/F): Pass 


Liquid Concentrate (25X) 

• 1X contains: Acetate (0.04M), 

EDTA (0.001M ) 

• pH 8.3 + - 0.1 


#730125 1x1L/pk $ 14.95 

#730127 10x1L/pk $ 56.35 

#730128 2x1L/pk $ 10.25 

#730137 1L /pk $ 39.77 

#730131 2x1L/pk $ 59.50 

#730135 10x1L/pk $ 170.2 

#730129 1x40L/pk $ 59.50 

#730144 1L $ 39.50 

#730134 4L $ 78.00 

Liquid Concentrate (50X) 

Tris - for protein analysis 

Tricine-SDS Running Buffer (10X) 


#743150 500ml $ 28.50 

• 1X contains: Tris base (50mM), 

Tricine (50mM), SDS (3.5mM) 

#743151 1L $ 48.50 

pH 8.24 

Sample Buffer (4X) 

• Same as above 

• Each pack makes 50X of stated 

volume 

#730136 500ml $ 23.70 

#730138 1L $ 38.40 

#730140 4L $ 97.60 

• Tris-HCl 400mM, Glycerol (40%) 


• pH 8.6 

#742106 25ml $ 21.00 

#742108 50ml $ 31.50 

70 

> 

accelerate: electrophoresis

Tris-Borate Buffer 

Tris-Borate EDTA Sample (4X-6X) - for nucleic acid analysis 

• Tris base (270mM) 

• Boric Acid (270mM) 

4X- #700944 25ml $ 15.00 


#700945 50ml $ 25.00 

• Bromophenol Blue G (0.025%) 6X- #742144 25ml $ 19.00 

• Xylene Cyanol (0.025%) 


#742146 50ml $ 28.50 

Tris-Borate Urea Sample Buffer (2X) - for protein analysis 

• Tris base (45mM) 

• Boric Acid (45mM) 

• Urea (3.5m) 

• Bromophenol 0.005% 

• Xylene Cyanol 0.025% 


Urea Running Buffer (10X) 

• Tris base (0.89M) 

• Boric Acid (0.89M) 

• Urea (20mM) 

TG Buffer - for electroblotting proteins in Western Blots 

Liquid & Powder (10X) 

#742152 25ml $ 28.50 

#742154 50ml $ 47.50 

#700947 1L $ 16.00 

#700948 2L $ 28.00 

#700949 4L $ 50.00 


• 1X TG contains: Tris base 

#705923 1L $ 19.95 

(0.025M), Glycine (0.192M) #705925 2L $ 35.15 

• pH 8.3 

#705926 4L $ 66.50 

#730171 1x4L/pk powder $ 55.13 

#730165 2x1L/pk powder $ 27.85 

#730168 5x1L/pk powder $ 49.68 

#705929 1x40L/pk powder $ 26.70 

#705928 2x40L/pk powder $ 39.50 

TGS Buffer - for protein separations 

Tris Glycine - SDS (10X) 


• 1X solution contains: Tris 

#705932 1L $ 19.95 

(0.025M), Glycine (0.192M), #705935 2L $ 35.15 

SDS (0.1%) 

#705943 4L $ 66.50 

• pH (5X dilution of 

reconstituted buffer) 8 - 8.5 

#730175 5x1L/ready pk $ 31.05 


#705931 1x40L/ready pk $ 26.70 

#730173 2x1L/ready pk $ 35.64 

#705930 2x40L/ready pk $ 37.50 

Tricine-SDS Sample Buffer - for protein analysis 

Non-Reducing (2X) 

• Contains Tris -HCl, SDS (8%), 

Glycerol (20%), Coomassie Blue 

G (0.01%), pH 8.45 

Reducing (2X) 

• Contains Tris -HC (450mM), 

SDS (4%), Glycerol (10%), 

Coomassie Blue G (0.0025%), 

β-mercaptoethanol (2%), pH 8.45 

Tris-Tricine (TT) Buffer - for nondenaturing & denaturing 

TT (10X) 


• pH (1X) @ 25ºC: 8.2-8.4 



#742102 25ml $ 19.00 

#742104 50ml $ 28.50 

#742148 25ml $ 19.00 

#742150 50ml $ 28.50 

electrophoresis of proteins 

#705969 1L $ 72.80 

#705970 1x1L/ready pk $ 64.70 

#705973 2x1L/ready pk $ 98.50 

buffers&reagents Molecular biology 

71 



Molecular biology 

TT SDS (10X) 


• pH (1X) @ 25°C 8.2 + 0.1 



• 1X Anode solution (pH 8.9): 

Tris-HCl (200mM) 

• 1X Cathode solution (pH 8.3): 

Zymogram Buffer - for protein analysis / use with zymogram 

gels to test for proteolytic activity 

Sample Buffer 

when characterizing proteins 

• 1X: Tris-HCl (63mM), pH 6.8, 

SDS (2%), Glycerol (10%), 

β-mercaptoethanol (2%), 

Bromophenol Blue ( 0.005%) 

Renaturation (10X) Buffer 

• 2.7% (w/v) Triton X-100 in 

distilled water 

- 

SDS Running Buffer (10X) 

Tris base (100mM), Tricine 

(100mM), SDS (0.1%) 

• TT-SDS Kits include both 

Cathode and Anode buffers. 

(cat. # 765544 & 765545) 

#705971 1L $ 56.02 

#705972 1x1L/ready pk $ 69.50 

#765540 500ml (anode) $ 19.95 

#765541 1L (anode) $ 35.15 

#765542 500ml (cathode) $ 38.00 

#765543 1L (cathode) $ 66.50 

#765544 500ml kit $ 47.50 

#765545 1L kit $ 85.50 

#742162 25ml $ 26.90 

#742164 50ml $ 37.50 

#765546 500ml $ 14.25 

#765547 1L $ 23.75 


General Use 

Acetate Buffer - for biological sciences 


• 1M Solution 

• DNase, RNase and Protease 

free 

pH 

#700610 4.0 500ml $ 23.75 

#700611 4.0 1L $ 37.50 

#700612 5.0 500ml $ 23.75 

#700613 5.0 1L $ 37.50 

Alkaline Phosphatase Buffer (30X) 

• Western Blot development 

• Microplate assays 

• Tris, Brij -35, NP - 40 

• pH 7.6 

• Stability: > 6 months at 4°C 

#730176 50ml $ 24.50 

#730177 100ml $ 39.60 

Ammonium Acetate Solution - reagent 

• MF: C 2 H 7 NO 2 

• MW: 77.08 

• CAS: 631-61-8 

• DNase, RNase and Protease 

free 


AMP-Buffer 

#705030 250ml $ 19.50 

#705032 500ml $ 35.00 

72 

Developing (10X) Buffer 

• 1X: Tris-HCl (50mM), pH 7.5, 

NaCl (200mM), CaCl 2 (5mM), 

Brij 35 (0.02%) 

#765548 500ml $ 19.95 

#765549 1L $ 37.15 

> 

accelerate: genonics/proteomics 

• MF: C 4 H 11 NO 

• MW: 89.14 

• CAS: 124-68-5 

• High Purity Grade 

• 2-amino-2-methyl-1-propanol 

• Purity (Anhydrous) >99% 

• pH 11.0 - 12.0 at 0.1M 


#705522 1L $ 39.60 

#705525 4L $ 115.20 

• Hazards: Irritant & Combustible 

• 5% water has been added to 

maintain liquid state at RT.

BES Buffer 

• MF: C 6 H 15 NO 5 S 

• MW: 213.2 

• CAS: 10191-18-1 

• N, N-Bis(2-hydroxyethyl), 

-2- Amino ethane sulfonic acid 

• Purity >99% 

• pKa range 6.4 - 7.8 

Borate Buffer 

• MF: H 3 BO 3 

• MW: 61.83 

• CAS: 10043-35-3 

• pKa at 20°C 9.24 

• Purity >99.5% 

• Sulfate



74 

DEPC-Treated Water -for ultimate RNA preparations 

• Ultra Pure Water 

• Molecular Grade 

#700799 250ml $ 18.70 

• Treated with - DEPC 

#710147 500ml $ 27.20 

#710148 1L $ 36.00 

EGTA - used in tissue culture media & biological buffers 

• Ethyleneglycol-bis 

(2-Aminoethylether)-N,N,N’,N’ 

tetraacetic acid 

• MF: C 14 H 24 N 2 O 10 

• MW: 380.35 

• CAS: 67-42-5 

• Purity (by titration) >97% 

• Melting point (with 

decomposition) 238°C 

Glycine Buffer - for biological sciences 

• MF: C 2 H 5 NO 2 

• MW: 75.07 

• CAS: 56-40-6 

• Purity>99% 

• Concentration (0.2M) 

• pH 3.0 

• A280 (water 1.0M)

PBS Dulbecco’s 

- for in vitro use 

• Modified washing liquid 

• Cell culture tested 

#700647 500ml $ 15.20 

• Store 2-8˚C 

PBS Tablets 


• Sodium Chloride (137mM) 

#700648 

#730091 

1L 

1x100ml 

$ 26.60 

$ 36.40 

• Phosphate Buffer (10mm) #730093 2x100ml $ 57.40 

• Potassium Chloride (2.7mm) 

• pH at 25˚C 7.4 ( + - 0.1) 



• Tablets dissolve within 3 

• Higher concentrations obtained 

by dissolving 2, 3 or 4 tablets in 

water (100ml) 

• To sterilize the solution for cell 

culture, autoclave or sterile filter. 

minutes with stirring 

PBS With Ca 2+ & Mg 2+ (10X) - for peroxidase conjugates 


• Sodium Phosphate (100mM) 

#700643 500ml $ 17.18 

• NaCl (9%) 

• Ca2+ & Mg2+ added 

• pH (at 25˚C) 7.4 

- for peroxidase conjugates 


• NaCl (9%) 

#700644 

#700650 

1L 

500ml 

$ 26.60 

$ 23.75 

• pH (at 25˚C) 7.4 

#700651 1L $ 39.90 

Potassium Chloride 

- reagent for molecular biology 

• MF: KCl 

• MW: 74.55 

#700878 100ml $ 13.50 

• CAS: 7447-40-7 

#700879 200ml $ 20.00 

• ACS Grade 


#700880 500ml $ 35.00 

• Purity 99.5% 

• pH at 25˚C water (5%) 5.4 - 8.6 


Potassium Phosphate - buffer for biological sciences 




• NaCl (9%) 


Sodium Acetate (sterile) 

pH 

#700654 7.2 500ml $ 23.75 

#700655 7.2 1L $ 39.90 

#700656 7.4 500ml $ 23.75 

#700657 7.4 1L $ 39.90 

• Autoclave Sterilized 

pH 

• 3M solution 

#700890 4.5 250ml $ 19.50 

• Storage: 4˚C 

#700891 4.5 500ml $ 35.00 

• Biotechnology grade 

#700893 5.2 200ml $ 25.94 

#700894 5.2 500ml $ 35.00 

buffers&reagents Molecular biology 

Potassium Acetate - biological buffer 

• MF: KC 2 H 3 O 2 

• MW: 98.14 

#700875 1 100ml $ 14.70 

• CAS: 127-08-2 

#700876 1 200ml $ 26.50 

• ACS Grade 

#700872 3 100ml $ 19.50 

• pH (at 25˚C, water 5%) 6.5 - 6.9 


#700873 3 250ml $ 35.00 


* M = molarity 

• Sodium



Sodium Borate 

• MF: BNaO 2 

• MW: 65.80 


• pH 9.0 (+/-0.1) 

Sodium Cacodylate Buffer 

• MF: C 2 H 6 AsNaO 2 

• 1M solution 

• High Purity (98%) 

• pH 6.8 ( + -0.1) 

Sodium Citrate Buffer 


• Buffer solution from Sodium 

citrate 


• pH 5.5 ( + 0.1) - 

#700679 500ml $ 37.50 

#700680 1L $ 55.60 

pH 

#700687 6.8 500ml $ 23.75 

#700688 6.8 1L $ 39.90 

#700690 7.0 500ml $ 23.75 

#700691 7.0 1L $ 39.90 

#700683 500ml $ 19.70 

#700684 1L $ 29.50 

Sodium Phosphate Buffer 

• Buffer solution from 

Phosphoric Acid / mono- 

#700693 5.8 500ml $ 19.70 

Sodium / DiSodium 

#700694 5.8 1L $ 29.50 

Hydrogen Phosphate 

#700696 6.0 500ml $ 19.70 


• Prepared under clean 

#700697 6.0 1L $ 29.50 

room conditions 

#700699 6.2 500ml $ 19.70 

• Limited Shelf Life 

#700700 6.2 1L $ 29.50 

• Used for protein purification, 

immunological assays and for #700702 6.4 500ml $ 19.70 

cell cultures 

#700703 6.4 1L $ 29.50 

#700705 6.6 500ml $ 19.70 

#700706 6.6 1L $ 29.50 

#700708 6.8 500ml $ 19.70 

#700709 6.8 1L $ 29.50 

#700711 7.0 500ml $ 21.70 

#700712 7.0 1L $ 32.50 

#700714 7.2 500ml $ 19.70 

#700715 7.2 1L $ 29.50 

76 

Sodium Hydroxide (10N) - reagent for alkaline lysis & pH 

• MF: NaOH 

#700900 100ml $ 17.50 

• MW: 40.00 

• CAS: 1310-73-2 

#700901 200ml $ 27.50 

• ACS Grade 

#700903 500ml $ 49.00 


• Sodium Carbonate

Sucrose Solution - reagent used for immunology 

• MF: C 12 H 22 O 11 

• MW: 324.30 

• CAS: 57-50-1 


• Purity >99.9% 

• 1.2M Solution 

• Specific rotation +66.3 - +66.8˚ 

• Glucose

Immunology buffers&reagents 

Imidazole Buffered Saline(IBS) Reagents 

IBS Casein 

• All blocks and diluents are 

supplied in a 10X concentration 

of physiological saline buffer. 

• pH 7.0 ( + 0.1) based upon an 

imidazole physiological buffer 

IBS Fish Gelatin 

• Gelatin (5%) 

• Diluents and coating 

solutions supplied as 10X 

• pH range 7.0 ( + 0.1) 

IBS Fish Gelatin-Glycerol 

- 


• pH range 7.0 ( + 0.1) 

- 

- 

#715383 2 x 100ml $ 47.50 

#715384 500ml $ 69.50 

#715390 2 x 100ml $ 62.70 

#715391 1L $ 149.00 

Bicarbonate Buffer 

• MF: NaHCO 

pH 

3 

#705728 8.0 

• MW: 84.0 

500ml $ 19.70 

• CAS: 144-55-8 

#705708 8.0 1L $ 34.50 

• 50mM Solution 

#705706 9.0 500ml $ 19.70 


#705707 9.0 1L $ 34.50 

#700233 10.0 500ml $ 19.70 

#700234 10.0 1L $ 34.50 

Blotto Blocker Buffer - to block non specific binding for 

Blotto / in PBS 

antibodies on the membrane 

• Nonfat dry milk (5%) 

• Sodium Azide in PBS (0.02%) 

#740031 500ml $ 21.70 

#740032 1L $ 37.50 

Phosphate Buffer Saline (PBS) Reagents 

PBS Casein (10X) 

• All blocks & diluents are 

supplied in a 10X PBS 

• pH range 7.4 ( + 0.1) 

- 

#715387 2x100ml $ 49.70 

#715338 500ml $ 69.50 

Blotto / Anti-Foam in PBS 



• Antifoam A (0.01%) 

Blotto / Tween in PBS 



• Tween (0.05%) 

#740078 250ml $ 23.75 

#740079 500ml $ 38.00 

#740082 250ml $ 21.70 

#740083 500ml $ 37.50 

PBS Fish Gelatin (10X) 


• 10X concentrate 

• pH range 7.4 ( + 0.1) 

- 

#715392 2x100ml $ 49.70 

#715401 500ml $ 69.50 

Blotto / in TBS 


• Sodium Azide in TBS (0.02%) 

Blotto / Anti-Foam in TBS 



• Antifoam A (0.01%) 

#740029 500ml $ 21.70 

#740030 1L $ 37.50 

#740084 250ml $ 23.75 

#740085 500ml $ 38.00 

78 

> 

accelerate: immunodetections

Blotto / Tween in TBS 



• Tween-20 (0.05%) 

#740087 250ml $ 21.70 

BSA Blocking Buffer - to block non specific binding site for 


BSA in PBS (10X) 

• BSA in PBS (3%) 

• Sodium Azide (0.02%) 

#740025 100ml $ 32.50 

#740026 200ml $ 49.70 

BSA in TBS (10X) 

• BSA in TBS (3%) 


#740027 100ml $ 32.50 

#740028 200ml $ 49.70 

Carbonate Buffer 

• MF: Na 2 CO 3 

• MW: 106.0 

#700235 500ml $ 19.50 

• CAS: 5968-11-6 

#700236 1L $ 27.50 


• Sodium Carbonate 

• 0.05M Solution 

• pH 9.4 

Gelatin Blocking Buffer 

Gelatin in PBS 

• Gelatin 0.1% 

#740066 500ml $ 28.50 

#740067 1L $ 52.25 

Gelatin in PBS with Azide 


#740068 500ml $ 28.50 


#740069 1L $ 52.25 


Immunology 

Casein Blocking Buffer - to block non specific binding 

site for antibodies on the membrane 

Casein in PBS 

• Casein PBS (3%) 

#740033 500ml $ 23.75 

#740034 1L $ 38.00 

Gelatin / Tween in PBS 

• Gelatin 10% 


• Tween 20 (0.05%) 

Gelatin in TBS 


#740098 500ml $ 27.50 

#740099 1L $ 47.50 

#740072 500ml $ 28.50 

Casein in PBS with Azide 

• Casein (3%) 


Casein in TBS 


Casein in TBS with Azide 



#740060 500ml $ 28.50 

#740061 1L $ 49.70 

#740035 500ml $ 28.50 

#740042 1L $ 43.50 

#740063 500ml $ 28.50 

#740064 1L $ 49.60 

Gelatin in TBS with Azide 



Gelatin / Tween in TBS 



• Tween 20 (0.05%) 

#740073 1L $ 49.70 

#740074 500ml $ 28.50 

#740075 1L $ 49.70 

#740100 500ml $ 27.50 

#740101 1L $ 47.50 

79


Horse Blocker 

• Horse serum (10%) 


#740104 100ml $ 33.25 

#740105 200ml $ 57.00 

Formaldehyde (4%) IN PBS - reagent for histology 



#730278 250ml $ 19.50 

#730279 500ml $ 35.00 

Protein Stripping Buffer (4X) - blot stripping buffer 

with Tris 



#740121 250ml $ 19.70 

• SDS (8%) 

#740122 500ml $ 34.50 

• Add ß-mercaptoethanol 

• pH at 25°C 6.8 


with Glycine 


• Glycine (200mM) 

#740123 250ml $ 17.80 

• pH at 25°C 2.5 

#740124 500ml $ 32.50 

Immunology 

80 

Para-Formaldehyde (4%) - reagent for histology 

In PBS 



In PBS with Mg 2+ , EGTA 



PBS-T (Tween 20) - phosphate buffered saline with Tween 20 to 

block non specific binding of proteins. 

PBS-T (Tween 0.5%) 10X 


• pH (at 25°C) 7.4 - 7.6 



PBS-T (Tween 1%) 10X 


• pH (at 25°C) 7.5 



#730280 250ml $ 27.60 

#730281 500ml $ 48.50 

#730282 250ml $ 27.60 

#730283 500ml $ 48.50 

#730104 250ml $ 23.75 

#730108 500ml $ 38.00 

#730110 500ml $ 23.75 

#730112 1L $ 38.00 

TBS-T (Tween 20) 10X - to block non specific binding site for 


• Tween 20 in 10x TBS 

(Tris Borate Saline) 


Capillary Electrophoresis 

Capillary Sequencing Buffer 

• Provided as 10x concentration. 

• Dilute to your working 

concentration. 

0.5%- #740092 500ml $ 21.50 

#740093 1L $ 37.50 

1%- #740090 500ml $ 21.50 

#740091 1L $ 37.50 

Formulated to be an effective running buffer when used with automated 

sequencers. This optimized buffer concentrate provides excellent results, 

long read lengths,and consistent run times. 

#700963 100ml $ 25.00 

#700965 500ml $ 450.00 

#700964 1L $ 120.00 

> 

accelerate: immunodetections

Tetraborate Buffer pH 9.9-10, 1mM 

Tricine Buffer pH 7.0, 1M 

Acetate Buffer pH 7.0, 1M 

CAPS Buffer pH 9.9-10, 1mM 

#700968 100ml $ 19.00 

#700969 250ml $ 39.00 

#700968 250ml $ 23.75 

#700969 500ml $ 39.00 

#700659 500ml $ 23.75 

#700661 1L $ 39.00 

#700966 100ml $ 19.00 

#700967 250ml $ 39.00 



CHAPS Lysis Buffer (1X) - for extracting protein from cells 




• CHAPS (1%) 

• See Cat#730039 for CHAPS 

DEA Buffer (10X) 

• Diethanolamine & MgCl 2 

• pH 9.5 

• Stability >1 year at 4°C 

#765531 250ml $ 33.25 

#765532 500ml $ 57.50 

#730148 50ml $ 28.40 

#730149 100ml $ 42.90 

Denaturing Lysis Buffer (1X) - for extracting proteins 


• SDS (2%) 

#765529 250ml $ 16.50 

#765530 500ml $ 27.00 

buffers&reagents Protein Purification 

Phosphate Buffer 

pH 4.4, 0.2M 

pH 6.8, 0.2M 

#700659 500ml $ 23.75 

#700661 1L $ 39.00 

#700662 500ml $ 23.75 

#700664 1L $ 39.00 

E. coli Lysis Buffer II - for protein extraction & purification 

of GST-fusion proteins 


• Triton X-100 (1%) 

pH 

#700202 7.4 250ml $ 19.50 


#700203 7.4 500ml $ 35.00 

• β-mercaptoethanol (10mM) 

#700200 8.0 250ml $ 19.50 

• KCl (2.7mM) 


#700201 8.0 500ml $ 35.00 

• See also Cell Buster BL Cat #506150 

> 



81



EIA Buffer (10X) Concentrate - makes 500ml of 1X 

solution when diluted with distilled water 

• Solution contains 1% BSA, 

#730139 50ml $ 24.40 

4M NaCl, 10mM EDTA and 

0.1% Azide 

• 1M Phosphate Solution 

• pH 7.4 

#730190 100ml $ 37.00 

• Stability >1 year at 4°C 

HEPES Buffer & Reagents 

HEPES 1M Sterile pH 7.3 


• MF: C 18 H 18 N 2 O 4 S 

#730048 100ml $ 18.27 

• DNAse, RNase, Protease free 

• pH (at 37°C) 7.2 - 7.4 


#730050 500ml $ 47.35 

• For HEPES Chemical (see page 106) 

HEPES Buffered Saline (2X) - for biological sciences 


• KCl (10mM) 

• Na 2 HPO 4 (1.5mM) 

• Dextrose (12mM) 

• HEPES (50mM) 

• pH 7.01 

Protein Extraction (5X) 


• CaCl 2 (5mM) 

• pH 7.4 

• Add protease inhibitor according to your protocol. 

#700620 250ml $ 28.50 

#700621 500ml $ 49.70 

- for extracting proteins from cells 

#765535 250ml $ 23.75 

#765536 500ml $ 37.50 

Gelatin (10%) - to block non specific binding site for antibodies on 

the membrane 

• Ready to use 

• Gelatin (10%) solution 

• For fish gelatin (see page 77) 

MES Buffer - used in plant cell culture 

• MF: C 6 H 13 NO 4 S 

• MW: 195.2 

• CAS: 4432-31-9 


• pH range 5.5 - 6.7 

• pKa at 25°C 6.10 ( + -0.05) 



• pKa value near physiological 

pH- not absorbed through cell membranes. 

• Essentially transparent to UV light 

• For MES Chemical (see page 107) 

 

 

 

 

#740096 500ml $ 33.25 

#740097 1L $ 57.00 

pH 

#700628 6.1 250ml $ 23.75 

#700629 6.1 500ml $ 39.90 

#700631 6.5 250ml $ 23.75 

#700632 6.5 500ml $ 39.90 

 

© 

Triton Protein Extraction 


• Triton X-100 (10%) 

• pH 7.4 

• Add protease inhibitor according 

to your protocol 

#765537 250ml $ 23.75 

#765538 500ml $ 38.00 

82

MOPS Buffer - for culturing cells in-vitro & other biological 

MOPS-1M pH 7.0 

applications 


• 3-(N-Morpholino) 

#700626 125ml $ 23.75 

Propane Sulfonic Acid 

#700627 250ml $ 39.90 

• MF: C 7 H 15 NO 4 S 

• pH range 6.8 - 8 

• pKa at 25°C: 7.0 - 7.4 



MOPS-EDTA (10X) 


• MOPS (0.4M) 

#730068 100ml $ 25.62 

• Sodium Acetate (0.1M) #730070 250ml $ 34.44 

• EDTA (0.01M) 

• RNAse: None 

#730072 500ml $ 49.50 

• DNAse: None 

• pH at 25°C 6.8 - 7.2 


• Prepared with DEPC-treated 

double distilled H 2 O for RNA 

applications 

NP-40 Lysis Buffer - for extracting proteins from cells 

No Salt 


#765523 250ml $ 23.75 

• Tris-HCl (50mM) 

• NP-40 (1%) 

#765524 500ml $ 38.00 

• EDTA (5mM), pH 8.0 

Protein Precleaning Buffer - for clarification of 

proteins to eliminate nucleic acids & polysaccharides before electrophoresis 

• For use with 1D gels 

• Storage: 20°C 

• Total protein preparation & 

cleaning solution 

PIPES Buffer 1M - for cell culture, in vitro & other 

biological applications 

• MF: C 8 H 17 N 2 O 6 S 2 *Na 1.5 

• MW: 335.37 

• CAS: 100037-69-2 


• pKa at 20°C 6.80 

• pH range 6.1 - 7.5 



#700635 100ml $ 25.75 

#700636 200ml $ 44.30 

• pKa value near physiological pH 

• Not absorbed through cell 

membranes 

• Essentially transparent to 

UV light 

Protein Extraction/Purification Buffer 

Extraction 



• Triton X-100 (1%), pH 8.0 

• For protein extraction & 

purification of GST fusion proteins 

#730026 10 x 1ml $ 29.95 

#730027 2 x 10ml $ 37.70 

#700206 250ml $ 19.50 

#700207 500ml $ 35.00 

buffers&reagents Protein Purification 

High Salt 



• NP-40 (1%), EDTA (5mM) 

• NaCl (500mM), pH 8.0 

#765525 250ml $ 23.75 

#765526 500ml $ 38.00 

E. coli Lysis 

• MF: Na 2 HPO 4 (50mM) 


• Imidazol (10mM), pH 8.0 

• For His-tag protein purification 

#700208 250ml $ 19.50 

#700209 500ml $ 35.00 

from E. coli & insect cells 

Low Salt 

70 



• NP-40 (1%) 

• EDTA (5mM), pH 8.0 

#765527 250ml $ 23.75 

#765528 500ml $ 38.00 

E. coli Wash 

• MF: Na 2 HPO 4 (50mM) 



#700210 250ml $ 19.50 

#700211 500ml $ 47.50 

83

E. coli Protein Elution 

RIPA Buffer - for extracting proteins from cells 



• Na 2 HPO 4 (50mM) 

#700212 250ml $ 19.50 



#700213 500ml $ 35.00 

• For His-tag protein purification from E. coli & insect cells 

Mammalian Protein Elution 

• Na 2 HPO 4 (50mM) 


#700217 250ml $ 19.50 

• Imidazol (250mM) 

#700218 500ml $ 35.00 

• Tween 20 (0.5%), pH 8.0 

• For His-tag protein purification from mammalian cells 

Mammalian Protein Wash 

• Na 2 HPO 4 (50mM) 

#700219 250ml $ 19.50 


• Imidazol (20mM) 

#700220 500ml $ 35.00 

• Tween 20 (0.5%), pH 8.0 

• For HIS-TAG protein purification from mammalian cells 

Protein Coupling Kit 

RIPA pH 7.4 



• NP-40 (2%) 

• Sodium Deoxycholate (1.0%) 

• SDS (0.2%) 

RIPA pH 8.0 (1X) 



• NP-40 (1%) 


• SDS (0.1%) 

RIPA with EDTA (1X) 



• NP-40 (1%) 


• SDS (0.1%) 

1X- #765512 250ml $ 29.70 

#765513 500ml $ 49.50 

2X- #765504 250ml $ 27.50 

#765505 500ml $ 44.70 

#765502 250ml $ 29.70 

#765503 500ml $ 49.50 

• EGTA (1mM) / EDTA (5mM) 

#765506 250ml $ 29.70 

#765507 500ml $ 49.50 

• EGTA (5mM) 

• pH 7.4 

• Gel swelling solution: 

#730265 1 Kit $ 93.00 

HCl (1mM) 

• Gel coupling solution-Sodium Borate or Citrate (100mM), 

NaCl (500mM), pH 9.3 

RIPA with EDTA & EGTA 



• NP-40 (1%) 

#765510 250ml $ 29.70 

#765511 500ml $ 49.50 

• Gel washing solution-Sodium Acetate (100mM), 


NaCl (500mM),pH 4.0 

• SDS (0.1%) 

• Kit is used for CnBr-activated Sepharose / Sepharophore 

• EGTA (1mM) / EDTA (5mM) 

Coupling. 

RIPA with Triton X-100 

• 1X - Tris-HCl (50mM), pH 7.4, 

NaCl (150mM), Triton X-100 

(1%), Sodium Deoxycholate 

1X- #765516 250ml $ 29.70 

#765517 500ml $ 49.50 

(0.5%), SDS (0.1%) 

• 5X - Tris-HCl (250mM), pH 7.4, 

NaCl (750mM), Triton X-100 

5X- #765514 250ml $ 29.70 

#765515 500ml $ 57.25 

(5%), Sodium Deoxycholate 

(2.5%), SDS (0.5%) 

84

Sodium Dodecyl Sulfate (20%) - ionic detergent used for 

denaturation of native proteins 

• MF: C 12 H 25 NaO 4 S 

• MW: 288.38 

• CAS: 151-21-3 


• Aggregation #: 62 


• A260 (3% water)



Protein Extraction (5X) 

• Tris-HCl (0.5M) 

• β-Mercaptoethanol (100mM) 


• pH 8.0 

• Add protease inhibitor according to your protocol 

Tris HCl (Sterile) 




* M = Molarity 

#765533 250ml $ 23.75 

#765534 500ml $ 38.00 

* M 

pH 

#730214 0.5 6.8 500ml $ 29.72 

#730206 0.1 7.4 100ml $ 17.43 

#730218 0.1 7.4 500ml $ 27.51 

#730199 1 7.5 100ml $ 23.52 

#730195 1 7.5 500ml $ 41.50 

#730212 2 7.5 1L $ 87.84 

#730213 2 7.8 500ml $ 44.85 

#730194 1 8.0 100ml $ 16.38 

#730197 1 8.5 500ml $ 18.27 

DTT (2M) 

• Ultrapure DTT for 

protein reduction. 

• Add an aliquot in buffer 

solution to maintain protein 

reduction potential. 

• Protease free for proteomics 

work. For solid DTT see Catalog# 705323, page112) 

#515939 5 x 1ml $ 37.40 

#515929 10 x 1ml $ 49.00 

#730198 1 9.0 250ml $ 21.48 

#730216 1 10 250ml $ 19.53 

Triton Cell Lysis Buffer - for extracting proteins from cells 




• Triton X-100 (1%) 


pH 

#765518 7.4 250ml $ 19.70 

#765519 7.4 500ml $ 34.50 

#765521 8.0 250ml $ 19.70 

#765522 8.0 500ml $ 29.70 

86 

> 

accelerate: proteinpurification


5-6 

Enzymes 

Inhibitors 

Substrates 

Antibodies 

> 

accelerate: lifescienceresearch

Enzymes & Substrates enzymes&substrates 


Enzymes 

Alkaline Phosphatase 

with Glycerol 

Cellulase (Onozuka R-10) 

for protoplast preparation 

• Often used in combination with Macerozyme R-10 

a-Chymotrypsin 

• Cleaves peptides on the C-terminus side of aromatic 

residues 

DNAse 1 (Bovine Pancreas) 

• CAS: 9003-98-9 

Galactosidase 

• Catalyzes the cleavage of beta-linked terminal 

galactose-containing substrates. 

Glucose Oxidase 

• Capable of oxidizing D-aldohexoses, monodeoxy-Dglucoses, 

and methyl-D-glucoses at varying rates. 

Horseradish Peroxidase 

• Horseradish Peroxidase is a single chain polypeptide 

containing four disulfide bridges and belongs to the 

ferroprotoporphyrin group of peroxidases. 

#752025 1ku $ 44.20 

#752027 2.5ku $ 94.60 

#752029 5ku $ 170.10 

#752021 1ku $ 35.60 

#752023 5ku $ 64.60 

#705117 1G $ 49.50 

#705119 5G $ 169.00 

#750033 1G $ 44.10 

#750035 5G $ 194.30 

#750048 50ku $ 119.70 

#750050 100ku $ 241.75 

#750137 1ku $ 34.70 

#750139 5ku $ 168.00 

#505168 up to 10mg $ 875.00 

#750057 50ku $ 17.00 

#750059 100ku $ 29.20 

#750061 500ku $ 116.60 

#750081 10ku $ 49.60 

#705797 25ku $ 64.20 

#705798 100ku $ 120.47 

Pectinase • Used in plant protoplast preparation 

to digest cell wall prior to organelle isolation. 

Pepsin 1:3000 (Porcine Source) 

• Preferentially cleaves C-terminal to Phenylalanine, 

Leucine and Glutamic Acid. 

Protein A 

Proteinase K 

• A serine proteinase used to inactivate RNAse and 

DNAse in RNA and DNA isolation protcols 

Solution 20mg/ml 

RNAse A 

• RNase A specifically hydrolyzes RNA between the 

3’-phosphate of a pyridine ribonucleotide and the 5’- 

hydroxyl of the adjacent nucleotide. 

S1 Nuclease 

Super Zymolyase 

T4 DNA Ligase 

FirePol 

Hot Fire Pol (TAQ) 

#705099 10G $ 72.20 

#705796 250G $ 34.65 

#705800 500G $ 63.36 

#700800 5mg $ 46.50 

#700802 20mg $ 150.00 

#700804 100mg $ 650.00 

#705873 100mg $ 72.24 

#705874 500mg $ 279.90 

#705875 1G $ 549.33 

#750085 5ml $ 85.50 

#750089 25ml $ 338.10 

#750092 250mg $ 112.20 

#750094 500mg $ 189.10 

#750096 1G $ 353.40 

#752104 100ku $ 209.00 

#513020 2x0.5ml $ 47.70 

#752176 20ku $ 47.50 

#752177 100ku $ 190.00 

#516222 1000u $ 79.00 

#516227 4000u $ 359.00 

#516220 1000u $ 99.00 

#516226 4000u $ 386.00 

en 

Lysozyme 

• Used in purification protocols of genomic and plas 

mid DNA to lyse bacterial cells. 

#750073 5G $ 24.75 

#750075 10G $ 35.20 

T4 Polynucleotide Kinase 

• Catalyzes the transfer of the g phosphate of ATP to 

a 5’-OH terminus in either RNA or DNA. 

#752179 0.5ku $ 23.75 

#752178 2.5ku $ 90.25 

88 

Macerozyme R-10 

• Used in plant protoplast preparation to digest cell 

wall prior to organelle isolation. 

Malate Dehydrogenase 

• Catalyzes the following reaction: Oxaloacetate + ß- 

NADH - L-Malate + ß-NAD 

#750076 25G $ 59.00 

#750082 100G $ 147.00 

#750080 1G $ 49.00 

#750084 5G $ 129.75 

#750077 25ku $ 38.40 

Tosyl Phenylalanyl 

Chloromethyl Ketone (TPCK) 

• MF: C 14 H 21 ClN 2 O 3 S HCl 

• MW: 369.31 

TPCK-Treated Trypsin 

#750120 50mg $ 9.00 

#750121 100mg $ 15.00 

#750122 250mg $ 35.00 

#750114 50mg $ 27.95 

#750115 100mg $ 45.50 

For In vitro lab use only - Not for human use or consumption

Trypsin 

• A serine peptidase that cleaves at internal peptide 

bonds on the carboxyl-side of arginine and lysine 

residues. 

1:250 Preparation 

1:300 Preparation 

#750108 25G $ 13.60 

#750117 50G $ 23.40 

#750119 250G $ 108.00 

#750109 25G $ 27.10 

#750110 50G $ 30.00 

#750111 1G $ 34.30 

#750113 5G $ 130.90 

AP 

Blue 

Yellow 

(Yellow= 405-410nm;Blue= 600-650nm) 

BCIP-5-Bromo-4-Chloro-3-Indoyl 

Phosphate 

INT 

• MF: C 8 H 6 BrClNO 4 P-C 7 H 9 N 

• MW: 433.62 

• CAS: 6548-06-9 

#715359 1 x 100ml $ 104.40 

#715358 2 x 100ml $ 92.70 

#715357 1 x 1L $ 166.50 

#715001 100mg $ 18.95 

#715003 500mg $ 71.98 

#715005 1G $ 122.30 

#715007 100ml $ 27.57 



Substrates 

ABTS-2,2’-Azino-bis (3-ethyl 

benzthiazoline-6-sulfonic acid) 

• MF: C18H24N6O6S4 

• MW: 548.66 

• CAS: 30931-67-0 

AP-Blue Stop Reagent 

RNAse Inhibitor (cloned) 

• Guaranteed nuclease-free and has been 

demonstrated not to inhibit nucleic acid 

modifying enzymes. 

• One unit of RNAse inhibitor inhibits the activity 

of 5ng of RNAse A by 50%. 

Stop Reagent 

#710991 1G $ 23.50 

#710992 10G $ 120.49 

#715351 2 x1 00ml $ 90.90 

#715350 1L $ 194.30 

#715370 1 x1 00ml $ 15.60 

#750099 2ku $ 72.60 

#527725 2.5ku $ 109.20 

#527728 5ku $ 198.00 

#750101 10ku $ 262.50 

Bluo-Gal 

NBT - blue 

Indoxyl-Gal 

NBT - purple 

Indoxyl-Glucoside 

• C 14 H 16 BrNO 6 

• MW: 374.2 

• CAS: 97753-82-7 

• C 14 H 17 NO 6 

• CAS: 126787-65-3 

• Purity >98% by TLC 

• C 14 H 17 NO 6 

• CAS: 487-60-5 


#715364 2 x 100ml $ 104.70 

#715363 1 x 1L $ 207.90 

#715366 2 x 100ml $ 104.40 

#715365 1 x 1L $ 209.00 

#714510 500mg $ 98.00 

#714511 1G $ 149.00 

#714513 500mg $ 98.00 

#714514 1G $ 149.00 

#714515 500mg $ 127.00 

#714516 1G $ 198.00 

Enzymes & Substrates 

405/410nm 

#715368 1 x 100ml $ 17.20 

450nm 

#715367 1 x 100ml $ 17.20 

> 

accelerate: enzymeassays 

89



IPTG (Isopropyl-ß-D- 

Thiogalactopyranoside) 

• C 9 H 18 O 6 S 

• MF: 238.31 

• CAS: 367-93-1 

D-Luciferin (Free Acid) 

Magenta-Caprylate 

• C 16 H 19 BrCINO 2 


MUG (4-Methylumbellifeyl-ß-D 

glucoronide-trihydrate) 

NBT Nitroblue tetrazolium chloride 

O-nitrophenyl-ß-D- 

Galactopyranoside 

O-Phenylenediamine 

(OPD) 

p-Nitrophenyl Phosphate 

#715051 1G $ 12.50 

#715053 2G $ 19.50 

#715055 10G $ 135.00 

#715054 25G $ 167.00 

#715074 50mg $ 330.00 

#715076 100mg $ 678.60 

#714512 1G $ 449.00 

#715267 100mg $ 23.40 

#715268 1G $ 196.50 

#715057 500mg $ 37.80 

#715059 1G $ 65.10 

#715061 5G $ 29.93 

#715063 25G $ 135.08 

#715075 25G $ 15.30 

#715077 50G $ 30.35 

#705778 100 tablets $ 78.91 

#705781 1000 tablets $ 461.63 

X-Glu (Glucopyranoside) 

X-Gluc 5-Bromo-4 Chloro-3-Indolyl-β-D- 

Glucuronide) 

Cyclohexylammonium Salt 

(For GUS assay) 

• C 16 H 13 BrCINO 6* C 6 H 13 N 

• MW: 521.8 

• CAS: 114162-64-0 

Sodium Salt Trihydrate 

X-Glucosaminide 

• C 16 H 18 BrCIN 2 O 6 

• MW 449.7 

• CAS: 4264-82-8 

X-α-Gal ( for β−galactosidase assay) 

• C 14 H 15 BrCINO 6 

• CAS: 107021-38-5 

X-α-Glucoside 

• C 14 H 15 BrCINO 6 


#715301 100mg $ 59.00 

#715303 500mg $ 199.00 

#715047 100mg $ 65.00 

#715049 500mg $ 198.00 

#715050 1G $ 349.00 

#714325 500mg $ 298.00 

#714502 500mg $ 349.00 

#714503 1G $ 547.00 

#714504 500mg $ 449.00 

#714505 1G $ 695.00 

#714506 500mg $ 495.00 

#714507 1G $ 749.00 

Salmon Gal 6-Chloro-3-Indolyl-β-D- 

Galactopyranoside 

#714192 100mg $ 116.64 

TMB-3,3’,5,5’-Tetramethyl benzadine 

#715353 2x100ml $ 92.70 

#715352 1x1L $ 203.40 

2 Component 

#715354 2x100ml $ 53.20 

Microwell 

#715361 100ml $ 29.70 

X-Gal (5-Bromo-4 Chloro-3-Indolyl-β- 

D-Galactopyranoside) 

#715041 200mg $ 10.98 

#715043 1G $ 44.95 

#715044 5G $ 211.95 

> 

accelerate: enzymeassays 

X-Galactosaminide 

#714500 500mg $ 395.00 

90 

• C 16 H 18 BrCIN 2 O 6 

• CAS: 129572-48-1 


#714501 1G $ 687.00 


Recombinant Protein Horomones 

Human Growth Hormone Releasing Hormone 

Human Follicle Stimulating Hormone 

Human Goserelin 

Human Leuprolide 

Human Leutenizing Hormone Releasing 

Hormone (Gonadorelin) 

Human Octreotide 

#507805 250µg $ 35.00 

#507799 10µg $ 65.00 

#507817 5mg $ 55.00 

#507826 5mg $ 38.00 

#507820 10mg $ 35.00 

#507838 1mg $ 35.00 

Chemokines 

Recombinant Human Gro-Alpha (CXCL1) 

Monocyte Chemotactic Protein-1 (CCL2) 



Recombinant Human Lymphotactin 

Recombinant Human MIG (CXCL9) 

#507679 25µg $ 90.00 

#507715 20µg $ 90.00 

#507718 10µg $ 90.00 

#507727 10µg $ 90.00 

#507712 10µg $ 90.00 

#507736 20µg $ 90.00 


Human Oxytocin 

Human Protirelin 

#507835 5mg $ 35.00 

#507850 100mg $ 70.00 

Recombinant Cytokines 

Recombinant Viral Proteins 

Recombinant Combined Hepatitis C Virus 

Recombinant Hepatitis C Virus NS5 Genotype 

Recombinant Cytomegalo Virus, Mosaic 

Recombinant Epstein-Barr Virus (HHV-4) P23 

Recombinant HepatitisC Virus NS5,Genotype 6 

Recombinant Rubella Virus Capsid C (91- 

123a.a.) 

Recombinant Dengue Virus NS3 

#508426 100µg $ 70.00 

#508534 100µg $ 70.00 

#508675 100µg $ 70.00 

#508846 100µg $ 70.00 

#508567 100µg $ 70.00 

#508876 100µg $ 70.00 

#508918 100µg $ 70.00 

Recombinant Human Artemin 

Recombinant Human Endocrine Gland 

Vacular Growth Factor 

Recombinant Human Epidermal 

Growth Factor 

Recombinant Human Granulocyte 

Macrophage- Colony Stimulating Factor 

Recombinant Human Baff (BLYS) 

Recombinant Human Beta- Nerve 

Growth Factor 

Recombinant Human Glial-Derived 

Neurotropic Factor 

Recombinant Human Growth Hormone 

#507181 10µg $ 90.00 

#507214 10µg $ 90.00 

#507217 500µg $ 90.00 

#507565 10µg $ 90.00 

#507187 20µg $ 90.00 

#507199 20µg $ 90.00 

#507250 10µg $ 90.00 

#507262 500µg $ 90.00 


Recombinant Hemagglutinin 

#508882 100µg $ 160.00 

Recombinant Human Interferon-Alpha 2A 

#507304 100µg $ 90.00 

Recombinant Hepatitis B Virus HBE 

Recombinant HIV-I GP41 

Recombinant HIV-I P24 

#508621 100µg $ 70.00 

#508744 100µg $ 70.00 

#508729 100µg $ 70.00 

Recombinant Human Neurotrophin-3 

Recombinant Human Prolactin 

#507571 10µg $ 90.00 

#507583 50µg $ 90.00 

Recombinant HIV-II Envelope 

#508816 100µg $ 70.00 

Recombinant Human Soluble CD4 

#507592 100µg $ 440.00 

Recombinant HIV-II GP32 

#508777 100µg $ 70.00 

Recombinant Human Thrombopoietin 

#507634 10µg $ 90.00 

Recombinant HIV-II GP36 

#508786 100µg $ 70.00 

Recombinant HTLV-I Enveope 

Recombinant Human HIV-I P55 GAG 

Recombinant Rubella Virus E1 Mosaic (157 

176, 374-390, 213-239a.a.) 

Recombinant Varicella Zoster Virus ORF26 

Recombinant Varicella Zoster Virus ORF9 

#508822 100µg $ 70.00 

#508810 100µg $ 210.00 

#508870 100µg $ 70.00 

#508684 100µg $ 70.00 

#508681 100µg $ 70.00 

For more recombinantproteins 

visit: www.liveproteins.com 

91

Enzymes & Substrate enzymes&substrates 

Recombinant Enzymes 

Recombinant Human AKT1 

Protein Kinase AKT1/PKB Alpha 

Active Enzyme 

Recombinant Human ERK1/MAPK1 

Active Enzyme 

Recombinant Human P38 ALPHA/SAPK2 

Alpha 

Recombinant Human Protein Kinase 

Alpha 

Recombinant Human Hormones 

Recombinant Human Chorionic 

Gonadotropin 

Recombinant Human Glucagon 

Recombinant Human Thyroid Stimulating 

Hormone 

#507955 2µg $ 35.00 

#507988 5µg $ 275.00 

#508006 1µg $ 255.00 

#508045 20µg $ 240.00 

#508060 2µg $ 35.00 

#507790 10µg $ 35.00 

#507814 100µg $ 35.00 

#507853 10µg $ 125.00 

Recombinant Human Proteins 

Recombinant Human Sumo-I 

Recombinant Human Heat Shock Protein 27 

Recombinant Human CD34 





Recombinant Human P53 

#508216 20µg $ 70.00 

#508909 2µg $ 35.00 

#508276 2µg $ 140.00 

#508279 2µg $ 140.00 

#508282 2µg $ 35.00 

#508903 2µg $ 35.00 

#508906 2µg $ 35.00 

#508315 2µg $ 140.00 


Antibodies 

Protein Phosphorylation on serine, threonine and tyrosine is an important 

reversible modification on many proteins for both enzymatic activation and in 

signal transduction pathways. These peptides can be detected after in vitro 

For more Recombinant Proteins 

visit www.liveproteins.com 

labeling or by Western blot analysis using appropriate antibodies. 

Anti-Phosphoserine 

Recombinant Proteins 

Recombinant Single Chain Cardiac 

Troponin I-C 

Recombinant Toxoplasma GONDII P29 

(GRA 7) 

#508231 2µg $ 45.00 

#508693 100µg $ 70.00 

• Antigen: phosphoserine 

containing synthetic peptide 

#650121 100µg $195.00 

• The polyclonal antibody was developed in rabbits and shown by ELISA 

to be high titer. 

Recombinant Toxoplasma GONDII P30 

(SAG1) 

#508696 100µg $ 70.00 

• This antibody is expected to react strongly with mouse NIH3T3 cells, 

human KL562 cells and human FGF-stimulated A431 cells. 

Recombinant Treponema Pallidum P15 


#508708 100µg $ 210.00 

#508705 100µg $ 70.00 

• Usage: Western blotting, immunoprecipitation, ELISA and affinity 

adsorption of phosphothreonine containing peptides. A 700-900 fold 

dilution is recommended for Western blot analysis. 


#508711 100µg $ 70.00 

• Storage: -20° C and avoid freeze/thaw cycles 

92 


#508714 100µg $ 70.00

Anti-Phosphotyrosine 

• Antigen: phosphoserine #650120 100µg $195.00 


• The polyclonal antibody was developed in rabbits and shown by ELISA 








Epitope Tag Antibodies 

• For Western blot analysis and ELISA dilute 1:800-1000 

• For immunoprecipitation dilute 1:100 

• For histological studies dilute 1:500 

• Storage: -20ºC, avoid freeze/thaw cycles 

Anti-MYC-Tag 

• Host: (rabbit) 

• Immunnogen MYC tag 

#650130 0.1mg $249.00 

Sequence (EQKLISEEDL) 

#650132 0.5mg $375.00 

Anti-FLAG-Tag 


Anti-Phosphothreonine 

• Antigen: phosphoserine #650122 100µg $ 195.00 


• The polyclonal antibody was developed inr abbits and shown by ELISA 








• Host: Rabbit 

• Immunogen: Synthetic Peptide 

(DYKDDDDKC ) 

Anti-S-Tag 

• Immunogen S-tag epitope tag 

Anti-HA-Tag 

• Host: Mouse monoclonal 

antibody 

• Immunogen HA-Tag (YPYDVPDYA) 

Anti-His 6 -Tag 

#650133 0.2mg $245.00 

#650129 0.2mg $ 245.00 

#650134 0.2mg $ 245.00 


Anti Fluorescent Protein Antibodies 

• Host: Mouse monoclonal #650135 0.2mg $ 245.00 

antibody 

• Immunogen His 6 -Tag (YPYDVPDYA) 

Anti-GFP 

#650140 20µl $ 352.50 

• Polyclonal antibody (Rabbit) against GFP (Green fluorescent 

protein) with high specificity for localization of GFP-tagged protein. 

• Suitable for immunoprecipitation at a dilution of 1:50 

• Storage: -20ºC, avoid freeze/thaw cycles 

Poly Histalon 

• Polyclonal antibodies (Rabbit) 

against poly Histidine with high 

specificity suitable for 

histochemical localization of the 

target protein with His epitope. 

#600052 200µg $ 44.50 

#600055 500µg $ 68.00 

#600057 1mg $165.00 

93

Enzymes & Substrate enzymes&substrates 

94 

Cytox Cytotoxicity Assay Kit, LDH 

• Simple & safe to use in any 

#526102 1000 assay $ 177.35 

set up. 

• Detects low level damage to cell membranes that are not detected with 

other methods. 

• Can be used with both suspension & adherent cells. 

• Non-radioactive - for convenient & high-throughput screening of cell 

death & cytotoxicity of test compounds. 

• Test involves a 96-well colorimetric assay & requires only the use of a 

standard plate reader. 

• Suitable for chemical as well as cell mediated cytotoxicity 

without the concern for safety involved in radioactive handling. 

• Produces results comparable to the values obtained with 

51Cr release assays. 

• Researchers can quantitate the color produced by reading the plate 

with a standard plate reader. 

• Results can be expressed as percentage cell survival or cell death. 

Diaminobenzidine Substrate System 

• Complete Peroxidase system. 

#715031 200mg $ 195.00 

• For Horseradish Peroxidase that 

has a greater sensitivity in the presence of Nickel or Cobalt. 

• Contains: DAB Substrate, Peroxidase Solution, Mixing/ 

Dispensing Bottle. 


Malachite Green Phosphate Assay Kit 

• Fast, convenient, robust and 

#600041 2,500 assays $187.00 

highly sensitive assay for 

phosphatase, phospholipase, and many more assays. High-throughput 

screen for phosphatase enzyme inhibitors. 

P-Nitrophenyl Phosphate for Phosphatase Assay 

Substrate only 

#715319 100ml $ 50.00 

#715320 500ml $ 135.00 

#715321 1L $ 245.00 

Complete Kit 

#600042 500 assays $185.00 

• This is an excellent substrate for phosphatase based ELISA 

assays. Stable for 18 months at 2-8ºC. It produces a soluble end 

product which is yellow in color and read at 405 nm to 410 nm. 

• Both kinetic and end point determinations can be performed. 

• This product is buffered in DEA. 

• Kit performs a simple, robust and highly sensitive assay for phosphatase 

activity, enzyme kinetics and high-throughput screen for phosphatase 

inhibitors. 

TMB ( Peroxides/Substrates) 

TMB-M 

Produces a soluble end product 

which is blue in color ideal for 

immunology procedures. 

TMB-S 

#715313 100ml $ 50.00 

#715314 500ml $ 85.00 

#715315 1L $ 150.00 

TMB-US #715310 100ml $ 50.00 

Ultra Sensitive TMB. Our fastest 

acting TMB for ELISA Incubate 2-15 

minute and read the blue color at 

#715311 500ml $ 95.00 

#715312 1L $ 175.00 

650 nm. Stop solution produces a yellow end product that can be read at 

450 nm. The yellow color is stable for up to 2 hours! 

Produces a soluble end product 

which is blue in color that is 

quantitated at 650 nm. Upon 

addition of stop reagent, the 

resulting yellow chromogen is 

quantitated at 450 nm. 

#715316 100ml $ 50.00 

#715317 500ml $ 85.00 

#715318 1L $ 150.00 

#71535 1L $ 45.00 

stop reagent

iochemicals 

7 

Antibiotics 

bioDetergents 

Research Chemicals 

> 


iochemicals 

Antibiotics 

Amphotericin B 

Antibiotics biochemicals 

Actinomycin D 

• CAS: 50-76-0 

• MW: 1255.42 



• Antineoplastic antibiotic 

produced by growth of 

Streptomyces chrysomallys & 

Amoxicillin 

with Potassium Clavulanate 

• Inhibitor of bacterial cell 

wall synthesis with high 

activity against Gram 

negative bacteria 

• Mixture contains 5 to 1 

amoxicillin to potassium 

#762003 5mg $ 49.50 

#762005 10mg $ 89.60 

#762007 25mg $ 198.70 

other species of Streptomyces. 

• Inhibits DNA replication 

#762200 2G $ 39.80 

#762201 5G $ 87.70 

• β-lactamase sensitive 


• Store at 2-8ºC 

• MF: C 47 H 73 NO 17 

• MW: 924.08 

• CAS: 1397-89-3 

• USP Grade 

• Antifungal 


• Store at 2-8ºC 

• Eliminates yeast and 

mold in cell cultures by means 

of disrupting the sterols of the 

cell membrane. 

Sterile 

• Tissue Culture Grade 

• Antifungal 

• Potency: 900 µg/mg 

• Solubility: 2.5 mg/ml 

• Residue upon ignition 

accelerate: cellcultureresearch 



activity against Gram 


• May cause sensitization by 

inhalation and skin contact 

• Irritating to eyes, respiratory 

system and skin 

96 

fresh antibiotics for microbial / animal / plant cell cultures

Ampicillin (contd.) 

Trihydrate 

• MF: C16H19N3O4S • 3H2O 

• MW: 403.66 

#762012 25G $ 29.00 

• CAS: 7177-48-2 

#762013 50G $ 53.20 

• Potency: 900-1050 µg/mg #762015 100G $ 87.95 

• USP Grade 

• Moisture (12-15%) 

#762017 500G $ 297.60 

• pH water (1%) 3.5 - 6.0 




activity against Gram negative 

bacteria 

• Selective agent for plasmid 

transformation such as pBR322 

& pUC derivatives 

Apramycin Sulfate 

• MF: C 21 H 41 N 5 O 11 

• MW: 637.66 

#762204 1G $ 25.00 

• CAS: 65710-07-8 

#762205 5G $ 87.90 



bactericidal activity against 

most Gram negative bacteria 

• Aminoglycoside antibiotic with • Harmful by inhalation, contact 

with skin & if swallowed 

Bacitracin 

Bleomycin Sulfate 

• MW: 1400 

• CAS: 9041-93-4 

#762018 5mg $ 189.70 

• Potency: 1 unit per mg solid #762019 15mg $ 355.00 

• Tissue culture tested 



• MP: 160˚F 

• Mixture of basic 

antineoplastic antibiotics 

produced by Glycopeptide 

Streptomyces verticullus 

• Binds to DNA & causes 

strand scissions 

• Carcinogen & Teratogen 


inhalation & skin contact 

Carbenicillin Disodium Salt 

• MF: C 17 H 16 N 2 O 6 SNa 2 

• MW: 422.37 

#762021 1G $ 23.70 

• CAS: 4800-94-6 

#762023 5G $ 56.60 

• α-carboxybenzylpenicillin #762025 10G $ 89.00 

• Potency>770 µg/mg 

• USP Grade 

#762024 25G $ 198.00 

• Hygroscopic 

• Protect against moisture 




• Interferes with bacterial cell 

wall formation 




biochemicals 

Antibiotics 

• MF: C 66 H 103 N 17 O 16 S 

• MW: 1422.69 

#762206 5G $ 52.50 

• CAS: 1405-87-4 

#762207 10G $ 81.90 

• Approximately 75,000 units/gm 

• Protect from moisture 

• Soluble in water 




activity against Gram positive 

bacteria 

• Toxic to plant cells 

• DO NOT breath dust 

• Avoid contact with skin & eyes 

Carboxin 

• MF: C 12 H 13 NO 2 S 

• MW: 477.5 

• CAS: 5234-68-4 



• MP: 91-92˚C 

#762026 250mg $ 142.75 

• Fungicide that inhibits the 

oxidation of succinate in 

sensitive yeast and fungi 

• May cause sensitization by inhalation 

> 


97 

For in vitro lab use only - Not for human use or consumption

Cefotaxime Sodium 

Chlorhexidine Digluconate 


• MF: C 16 H 16 N 5 O 7 S 2 Na 

• MW: 477.5 

#762076 5G $ 62.70 

• CAS: 64485-93-4 

#762077 10G $ 95.70 


#762078 20G $ 172.00 


• Inhibits synthesis of bacterial 

#762079 50G $ 389.50 

cell wall 



inhalation 

Cephalexin Monohydrate 

• MF: C 16 H 17 N 3 O 4 S*H 2 O 

• MW: 365.4 

#762208 5G $ 46.25 

• CAS: 15686-71-2 




• Active against Gram 

positive bacteria 

• Moderately active against 

Gram negative bacteria 

• Inhibitor of cell wall synthesis 


• Harmful by inhalation, contact with 

skin or if swallowed 

• May cause irritation to eyes, 

• MF: C 22 H 30 Cl 2 N 10 *2C 6 H 12 O 7 

• MW: 897.76 

• CAS: 18472-51-0 

• Aqueous solution (20%) 

• Bisbiguanide antiseptic 

& disinfectant 

• Inhibits mycobacteria, fungi, 

& some viruses 


Chlortetracycline HCl 

• MF:C 22 H 23 CIN 2 O 8 *HCl 

• MW: 515.34 

• CAS: 64-72-2 


• Protect from light 


• Melting point: 210-215˚C 

#714134 10ml $ 35.00 

• Bactericidal & Bacteriostatic 

against Gram positive & 


• Active: neutral or slightly acid pH 

• Clean instruments & hard surfaces 

in a concentration of water 

(0.05% to 0.5%) 

#762210 25G $ 75.00 

• Bacteriostatic antibiotic active 

against Gram positive & Gram 



Chloramphenicol 

• MF: C 11 H 12 Cl 2 N 2 O 5 

• MW: 323.13 

• CAS: 56-75-7 

• Potency > 900 µg/mg 


• pH water (2.5%): 4.5 - 7.5 


• Light sensitive 

• Bacteriostatic against Gram 

negative and Gram 

positive bacteria 

• Inhibits protein synthesis 

by preventing peptide bond 

respiratory system & skin 

#762027 50G $ 35.00 

#714132 100G $ 65.00 

#762029 250G $ 127.50 

formation by peptidyl transferase 

• Used in large scale plasmid 

preparation of low to 

moderate copy number 

plasmids & in CAT assays 




Clindamycin HCl 

• MF: C18H33CIN2O5S*HCl* 

• MW: 461.45 

• CAS: 21462-39-5 



Colchicine 

• MF: C22H25NO6 

• MW: 399.44 

• CAS: 64-86-8 


• Hazard: Very Toxic 

#762213 500mg $ 79.00 

#762212 1G $ 129.50 

• Lincosamide antibiotic active 

against Gram positive bacteria 


#714144 100mg $ 63.40 

• MP: 150-160˚C 


• Inhibits tubulin polymerization 

98 


Destomycin 

G-418 Disulfate (Geneticin®) 

• MF: C20H37O13 

• MW: 527.5 

• 50% Destomycin 

50% Rice starch 



• Used as an alternative to 

#762216 100mg $ 187.50 

Hygromycin B - less toxic 

• Similar to Hygromycin B - 

phosphorylated with the 

same enzyme 

• MF: C 20 H 40 N 4 O 10 *2H 2 SO 4 

• MW: 692.71 

• CAS: 108321-42-2 

• Cell cultured tested 

• Ultra Pure 


• Aminoglycoside antibiotic 

used as a selective agent in 

#762037 250mg $ 59.50 

#762052 1G $ 89.70 

#762054 5G $ 259.00 

biochemicals 

Doxorubicin HCl 

• MF: C 27 H 29 NO 11 HCl 

• MW: 579.98 

• CAS: 25316-40-9 



• Harmful if swallowed 

• Light/moisture sensitive 

Doxycycline HCl 

• MF: C 22 H 24 N 2 O 8 *HCl 

• MW: 480.9 

• CAS: 10592-13-9 




• Active against Gram positive/ 

#762218 10mg $ 137.50 

• Possible risk of 

irreversible effects 

• Antineoplastic antibiotic interferes 

with nucleic acid synthesis - acts on 


#762220 10G $ 84.50 


• Interferes with bacterial cell 

wall synthesis 

• Harmful by inhalation, contact 

with skin & if swallowed 

transformation experiments 

with animal, plant and 

yeast cells. 

Gentamicin Sulfate 

• CAS: 1405-41-0 

• Approximately 650 units/mg 

• USP Grade 

• Potency


Hygromycin B 

• MF: C 20 H 37 N 3 O 13 *HCl 

• MW: 564.0 

• CAS: 31282-04-9 

• Purity: 60% minimum by HPLC 



• MP 160-180˚C 

• Inhibits peptide synthesis by 

blocking peptide chain 

elongation 

• Source: Streptomyces 

Kanamycin Sulfate 

• MF: C 18 H 36 N 4 O 11 *H 2 SO 4 

• MW: 582.58 

• CAS: 25389-94-0 

• Potency >750 µg/mg 

• pH water (1%) 6.5 - 8.5 




#762047 50mg $ 19.75 

#762049 100mg $ 26.95 

#762051 250mg $ 57.60 

#762053 1G $ 97.20 

hygroscopicus 

• Toxic by inhalation or if swallowed 

• Irritating to skin and eyes 

#762055 10G $ 49.50 

#762057 25G $ 96.00 

#762056 50G $ 136.70 

#762059 100G $ 316.60 

• Used as a selective 

agent for the incorporation 

Methotrexate 

• MF: C 20 H 22 N 8 O 

• MW: 454.44 

• CAS: 59-05-2 



• Antineoplastic agent acts as 

an antimetabolite of folic 

acid biosynthesis 

• Inhibits the enzyme 

Dihydrofolate Reductase & 

Metronidazole 

• MF: C 6 H 9 N 3 O 3 

• MW: 171.15 

• CAS: 443-48-1 

• Soluble in diluted acids 

& DMFO 



• MP: 154-161˚C 

#762058 500mg $ 127.00 

#762060 1G $ 228.50 

prevents formation of 

Tetrahydrofolate 

• Toxic by inhalation, contact with 

skin & if swallowed 

• May cause heritable genetic damage 

#762062 25G $ 56.25 

#762064 100G $ 128.70 

• Derivative of 5-Nitroimizadole 

with activity against anaerobic 

protozoa & bacteria. 

• Possible carcinogenic effects 

• Aminoglycoside with activity 

of NPT II (Neomycin 

against Gram negative bacteria 

• Binds to ribosomal subunit & 

Phosphortransferase) (APH3’) gene 

in plant & animal cells 

Miconazole Nitrate 

inhibits protein synthesis 

Lincomycin HCl 

• MF: C 18 H 34 N 2 O 6 S*HCl*H 2 O 

• MW: 461.02 

• CAS: 859-18-7 



#762222 10G $ 89.70 


• Bacteriostatic action against 

Gram positive bacteria 

• MF: C 18 H 14 Cl 4 N 2 O*HNO 3 

• MW: 479.14 

• CAS: 75319-48-1 

• Soluble in Propylene Glycol 


• Imidazole antifungal agent 

that interferes with 

Ergosterol synthesis 

#705302 1G $ 13.60 

#705303 5G $ 35.90 

#705304 25G $ 107.75 

• Alters the permeability of the 

cell membrane of sensitive fungi 

& yeasts 


100 


Mitomycin C 

Nystatin 

• MF: C15H18N4O5 

• MW: 334.33 

• CAS: 50-07-7 

• Soluble in ethanol 

• Slightly soluble in water 


• Acts as an alkylating agent 

after activation & suppresses 

#714320 2mg $ 71.40 

the synthesis of nucleic acids 

• Cell cycle non-specific agent 

• Most active in the late G1 & early S 

phases 

• Hazard: Highly toxic, possible 

carcinogen 

• MF: C 47 H 75 NO 17 

• MW: 926.1 

• CAS: 1400-61-9 

• Potency >4400 U/mg 

• pH (3%water suspension): 

6.5 - 8.0 


• Moisture 98% 


• Moisture: 105˚C 600 µg/mg 

• pH water (3%) 5.0 - 7.5 

8.0 - 10.5 

• Moisture

Penicillin (contd.) 

G, Sterile 

Phleomycin 


• Cell wall inhibition 

• Reconstituted in sterile water 

(20ml) - yields Penicillin G 

(10,000 U/ml) 

• Gamma irradiated 

V, Sodium Salt 

• MF: C 16 H 17 N 2 NaO 4 S 

• MW: 356.37 


• Potency 1650 U/mg 

• Water Soluble 

Streptomycin Sterile 

• 10mg/ml 


• Reconstitution in distilled 

water (20ml), yields 

Penicillin G (10,000U/ml) 

#705790 20ml $ 14.50 



#762224 25G $ 19.50 


• Cell wall synthesis inhibitor 

• Active against Gram positive 

& some Gram negative bacteria 

#705792 20ml $ 16.65 

• Irradiated 

• Storage: -20°C 

• MF: C 9H 11 NO 2 

• MW: 165.2 

• CAS: 11006-33-0 



• Produced by Streptomyces 

verticullus 

• Selective agent in 

transformation experiments 

with mammalian, plant & 

yeast cells 

Polymyxin B Sulfate 

• MF: C 55 H 96N 16 0 13 *2H 2 SO 4 

• MW: 1385.61 

• CAS: 1405-20-5 

#762226 25mg $ 39.70 

• Cytotoxic action results from 

the ability to cause fragmentation 

of DNA 

• In vitro studies indicate Phleomycin 

causes accumulation of cells in the 

G2 phase of the cell cycle 



#762073 25mu $ 52.10 

#762074 100mu $ 179.50 

Streptomycin Amphotericin B 

• Potency >6000 U/mg 


• 10mg Streptomycin & 2.5mg 

Amphotericin B per ml 

#705793 20ml $ 16.65 

• Loss on drying

Ribavirin 

• MF: C 8 H 12 N 4 O 5 

• MW: 244.20 

• CAS: 36791-04-5 



Rifampicin 

• MF: C 43 H 58 N 4 O 12 

• MW: 822.95 

• CAS: 13292-46-1 

• Refamycin SV 3-(Methyl-4 

Piperazinyl Inino-1) 

• Soluble in dilute acid solution 

• USP Grade 

• Bright red or dark red 

Spectinomycin Dihydrochloride, Pentahydrate 

• MF: C 14 H 24 N 2 O 7 *2HCl*5H 2 O 

• MW: 495.3 

• CAS: 21736-83-4 



• Irritating to eyes & respiratory 

Streptomycin Sulfate 

• MF: (C 21 H 39 N 7 O 12 ) 2 *3H 2 SO 4 

• MW: 1457.41 

• CAS: 3810-74-0 

• Potency >650 µg/mg 

• USP Grade 

• pH water (20%) 25˚ 4.5 - 7.0 

• Loss on drying 900 µg/mg 

#762089 100G $ 65.80 

• pH water (1%) 1.8 - 2.8 

• Soluble in Ethanol 


• Bacteriostatic antibiotic with 

activity against Gram positive/ 


Aminoacyl transferase 

• Active marker for the 

transformation of plasmids 

encoding for tetracycline resistance 

• Irritating to eyes, respiratory system 

& skin 

• Binds the 30S subunit of the 

ribosome preventing binding of 

Ticarcillin Disodium Salt 

• MF: C 15 H 14 N 2 Na 2 O 6 S 2 

• MW: 428.4 

#762234 1G $ 58.90 

• CAS: 4697-14-76 

#762235 5G $ 111.00 



• Inhibits bacterial cell 

wall synthesis 

• Active against Gram negative 

bacteria (such as Agrobacterium 

strains) and thus used for plant cell 

transformation 




antibiotics for in vitro lab use only. 

103 

biochemicals 

Antibiotics

Vancomycin HCl 

biochemicals 

Timentin 

• Ticarcillin Disodium Salt/- 

Potassium Clavulanate mixture 

• CAS (Ticarcillin Disodium) 

4697-14-76 

• CAS (Potassium Clavulanate) 

61177-45-5 

• 15:1 



#762236 2G $ 44.00 

#762237 5G $ 104.00 

• Plant cell culture tested 

• β-lactamase inhibitor 

• Active against Gram negative 

bacteria (such as 

Agrobacterium strains) 


• MF: C 66 H 75 Cl 2 N 9 O 24 *HCl 

• MW: 1485.7 

• CAS: 1404-93-9 

• Potency 1099 µg/mg 



• Used for plant transformation 

• Glycopeptide -inhibits 

formation of peptidoglycan 

polymers of the bacterial 

cell wall 

#762242 1G $ 89.00 

#762243 5G $ 198.00 

• Produces synergism in antimicrobial 

activity when combined with 

Cefotacime or Carbenicillin 

• Used by Agrobacterium species 

with high β-lactamase production 

• Storage: 2-8˚C in airtight container 

• Irritating to eyes, respiratory 

system & skin 

bio Detergents 

Tobramycin Sulfate 

• MF: (C 18 H 37 N 5 O 9 ) 2 *5H 2 SO 4 

• MW: 1425.4 

• CAS: 79645-27-5 


• Aminoglycoside that has a 

bactericidal action against 

Gram negative bacteria 

#714292 500mg $ 293.70 

• Inhibits protein synthesis & errors 

generated in the transcription of 

DNA 

• Storage (dry): 2-8˚C 

Virginiamycin 

• CAS: 11006-76-1 


• Natural mixture of two cyclic 

lactone peptolidesfactors 

M & S 

#762244 100mg $ 135.00 

• Storage (dry): 2-8˚C 

• Produced by Streptomyces virginiae 

• Harmful by inhalation, contact with 

skin & if swallowed 

Validamycin A 

• MF: C 20 H 35 NO 13 

• MW: 497.5 

#762240 1G $ 59.95 

• CAS: 37248-47-8 

#762241 5G $ 269.50 

• Soluble in DMSO & Ethanol 

• Inhibits Trehalase activity 

• Enhances Trehalose 

accumulation in Transgenic plants 


freshantibiotics@lowestprice 

Zeocin 

• MF: C 55 H 83 N 19 O 21S 2 Cu 

• MW: 1137.40 


• High toxicity in mammalian 

cells, plants, yeasts & bacteria 

• Acts by binding to DNA & 

cleaving it, causing cell death 

• Copper-chelated Glycopeptide 

isolated from culture broth of 

a Streptomyces verticillus 

mutant 

#706210 250mg $ 76.00 

#706211 1G $ 198.00 


104

iochemicals 

Detergents 

CAPS 

(3)Cyclohexylamino-(1)Propanesulfonic acid 

• MF: C 9 H 19 NO 3 S 

• MW: 221.32 

• CAS: 1135-40-6 

• pH range 9.7 - 11.1 

• pKa (20˚C) 10.40 



• pKa value near physiological pH 

Sodium Salt 

• MF: C 9 H 18 NO 3 SNa 

• MW: 243.3 




#730021 250G $ 51.20 

#730023 500G $ 92.19 

• Not absorbed through 


• Essentially transparent to UV light 


#730241 25G $ 15.40 

#730242 100G $ 43.55 

CAPSO 3-[Cyclohexylamino]-2-Hydroxy-1- Propanesulfonic acid 

Free Acid 

#730243 25G $ 14.50 

BRIJ-35 Polyoxyethylene lauryl Ether 

• MF: C 58 H 118 O 24 

• MW: 1199.57 

• CAS: 9002-92-0 

• Water 99% 

• Cationic detergent used in 

bacterial DNA purification & to 

precipitate DNA 


• MF: C 19 H 42 NBr 

• MW: 364.48 

#730031 500G $ 48.63 

• CAS: 57-09-0 

#730033 1kg $ 98.10 



DNA purification & DNA precipitation 


• Cationic detergent used for 

biochemicals 

bio Detergents 

#730244 100G $ 47.50 

CHAPS 

Sodium Salt 

#730267 25G $ 16.65 

#730268 100G $ 53.20 

• MF: C 32 H 58 N 2 SO 7 

• MW: 614.88 

• CAS: 75621-03-3 

• Conductivity water 

(10%)

io Detergents biochemicals 

106 

CHAPSO 

• MF: C 32 H 58 N 2 O 8 S 

• MW: 630.88 

• CAS: 82473-24-3 

• Nondenaturing Zwitterionic 

detergent 

• Solubilizing agent for 

membrane proteins 


DOC Deoxycholic Acid Sodium Salt 

• MF: C 24 H 39 Na 4 

• MW: 414.56 

• CAS: 302-95-4 

• Anionic detergent extracted 

from bovine bile 


#730041 1G $ 54.00 

#730043 5G $ 107.35 

#730045 10G $ 215.50 

CHES 2 -(N-Cyclohexylamino) ethane sulfonic acid 

• MF: C 8 H 17 NO 3 S 

• MW: 207.29 

• CAS: 103-47-9 

• pH range 8.6 - 10.0 

• pKa (20˚C) 9.55 


#730047 100G $ 34.40 

#730049 500G $ 141.75 



#730051 50G $ 29.40 

#730053 100G $ 46.75 

#730055 500G $ 194.78 


> 

accelerate: membraneresearch 

DIPSO 3-[N,N-bis(2-Hydroxyethyl)amino] 

• MF: C 7 H 17 NO 6 S 

• MW: 243.28 

#730245 25G $ 35.60 

• CAS: 68399-80-4 

#730246 100G $ 99.50 

• Melting Point 189-192˚C 

• Moisture (KF) 98% 


N-Dodecyl-β-D-Maltoaside 

• MF: C 24 H 46 O 11 

#730056 1G $ 115.90 

• MW: 510.60 

• CAS: 69227-93-6 

• Storage: Below 4˚C 

• Non-ionic detetgent similar to 

N-Octyl-beta-D-Glucopyranoside 

LDS Lithium Dodecyl Sulfate 

• MF: C 12 H 25 LiO 4 S 

• MW: 272.33 

• CAS: 2044-56-6 

• Denatures native proteins 

in gel electrophoresis 


NonIdet P-40 

• MF: C 16 H 26 O 2 

• MW: 603 (avg) 

• CAS: 9016-45-9 

• CMC: 0.29 

• Density (20˚C) 1.06 

• Solubility in water (5%) clear/ 

haze free 

• Refractive Index: 1.48-1.52 

#730529 25G $ 47.33 

#730531 100G $ 142.02 

#730533 250G $ 328.50 


#730079 50ml $ 13.98 

#730081 100ml $ 17.30 

#730083 500ml $ 55.65 

• Reagent Grade 

• Non ionic nondenaturing detergent 

used for solubilizing proteins 

• Storage: RT

N-Octyl β D-Glucopyranoside 

• MF: C 14 H 28 O 6 

• MW: 292.37 

#730087 1G $ 31.20 

• CAS: 29836-26-8 

#730089 5G $ 125.70 

• CMC: 20-25 


• Specific rotation -32 to-28˚C 

• Non-ionic detergent used to 

solubilize membrane proteins 

• Storage: -15 to -22˚C 

• Also known as n-octyl glucoside 

TritonX -100 /X-114 

• MF: C 34 H 62 O 11 

• MW: 646.86 

• CAS: 9002-93-1 

• Peroxides: None 

• Reagent Grade 

• Non-ionic nondenaturing 

100- #730208 500ml $ 13.70 

#730209 1L $ 21.50 

114- #730210 500ml $ 15.90 

#730211 1L $ 23.50 

biochemicals 

detergent 

membrane proteins 

SDS Sodium Dodecyl Sulfate 

• Used to solubilize 

• Used for isoelectric focusing 

• MF: C 12 H 25 NaO 4 S 

• MW: 288.38 

• CAS: 151-21-3 

• CMC 7-10 


• A 260 water (3%)

Research Chemicals biochemicals 

108 

biochemicals 

Research 

ACES - N-[Carbamoylmethyl]-2- 

Aminoethane-Sulfonic acid 

• MF: C 4 H 10 N 2 O 4 S 

• MW: 182.20 

• CAS: 7365-82-4 

Acetylsalicylic Acid 

Acriflavine, Neutral 

(EUFLAVIN) 

Acrylamide/ bisAcrylamide 

Mixture 

Bis 19:1 

Powder 

Bis 19:1 30% 

Bis 19:1 40% 

Bis 29:1 

Bis 29:1 30% 

Bis 29:1 40% 

Solution 

Solution 

Powder 

Solution 

Solution 

#730001 100G $ 49.61 

#730003 500G $ 230.37 

#730005 1kg $ 458.35 

#714110 500G $ 39.50 

#705009 100G $ 78.75 

#705011 250G $ 114.83 

#740011 500g $ 92.40 

#740013 1kg $167.60 

#705494 40G $ 26.25 

#705498 200G $ 80.75 

#705071 100ml $ 15.80 

#705060 500ml $ 42.60 

#705070 1L $ 79.00 

#705065 100ml $ 10.80 

#705076 500ml $ 36.00 

#705064 1L $ 68.00 

#705496 40G $ 26.20 

#705499 200G $ 89.25 

#705062 100ml $ 16.80 

#705072 500ml $ 43.60 

#705061 1L $ 80.00 

#705080 100ml $ 16.80 

#705066 500ml $ 43.60 

#705078 1L $ 80.00 

Bis 37.5:1 30% Solution 

Bis 37.5:1 40% Solution 

Adenine 

6-Aminopurine 

Hemisulfate 

• MF:(C 5 H 5 N 5 )•H2SO4•H2O 

Sulfate Dihydrate 

• CAS: 6509-19-9 

Adenosine 

9-D-Ribofuranosyladenine 

Adenosine 5’- 

Diphosphate (ADP) 


Monophosphate (AMP) 

disodium salt 


Triphosphate (ATP) 

AEBSF-4-(2 Aminoethly) benzene 

sulfonyl fluoride 

Agarose 3:1 

High RES Blend 

Bis 37.5:1 Powder #705497 40G $ 24.20 

• MW: 368.33 

• CAS: 321-30-2 

• MF: (C 8 H 10 FNO 2 )•HCL 

• MW: 239.70 

• MF: C 5 H 5 N 5 

• MW: 135.14 

• CAS: 73-24-5 

#705500 200G $ 76.80 

#705063 500ml $ 15.80 

#705074 1L $ 43.60 

#705068 100ml $ 15.80 

#740016 500ml $ 43.60 

#705013 50G $ 39.75 

#705015 100G $ 70.40 

#714101 25G $ 32.40 

#714100 50G $ 54.80 

#714102 100G $ 106.60 

#705014 50G $ 38.01 

#705017 50G $ 47.73 

#705019 100G $ 93.08 

#705021 5g $ 67.32 

#705057 25G $ 52.50 

#705059 100G $ 168.00 

#705077 25G $ 78.60 

#705079 100G $ 293.84 

#714112 50mg $ 33.95 

#714113 100mg $ 52.25 

#714111 1G $ 384.00 

#705460 25G $ 55.65 

#705461 100G $ 175.30 

#705462 250G $ 298.00 


Agarose 

Agarose II 

L-Alanine 

• CAS: 9012-36-6 

#740038 25mg $ 24.20 

#740039 100G $ 72.40 

#740040 500G $242.20 

#740050 25 G $ 81.05 

#740052 100G $240.10 

#710001 100G $ 26.00 

#710003 250G $ 59.07 

Phosphate (Dibasic) 

Phosphate (Monobasic) 

Sulfate 

#705041 1kg $ 47.25 

#705043 2.5kg $ 103.27 

#705045 500G $ 24.36 

#705047 1kg $ 37.80 

#705051 1kg $ 19.86 

#705052 2.5kg $ 47.50 

#705053 5kg $ 67.48 

#705055 10kg $ 81.70 

biochemicals 

Albumin Bovine 

Fraction V 

P-Aminobenzoic Acid (PABA) 

Ammonium 

Acetate 

Carbonate 

Chloride 

Hydrogen Carbonate 

Nitrate 

Persulfate 

#710005 500G $ 93.27 

#705020 25G $ 32.60 

#705023 100G $ 78.60 

#714178 25G $ 42.75 

#705025 500G $ 17.93 

#705026 1kg $ 30.70 

#705027 2.5kg $ 40.22 

#705521 1kg $ 26.36 

#705029 500G $ 17.00 

#705031 1kg $ 32.70 

#705033 2.5kg $ 58.00 

#714106 500G $ 22.75 

#714108 500G $ 33.25 

#714109 1kg $ 53.65 

#705035 25G $ 9.68 

#705037 100G $ 29.50 

#705038 500G $ 98.00 

AMPSO 3-[(1,1-Dimethyl-2) Hydroxyethyl 

amino]-2-Hydroxypropanesulfonic acid 

Free Acid 

L-Arginine 

Sodium Salt 

L-Ascorbic Acid 

(Vitamin C) 

L-Asparagine 

L-Aspartic Acid 

Avidin 

(recombinant grade 1) 

Benzamidine Hydrochloride 

Benzoic Acid 

• MW: 227.28 

• MW: 249.3 

• MF: C 6 H 14 N 4 O 2 

• MW: 174.20 

• CAS: 74-79-3 

• MF: C 7 H 8 N 2 •HCL•H 2O 

• MW: 156.62 + H 2 O 

• CAS: 1670-14-0 

• MF: C 6 H 6 O 2 

• MW: 122.12 

• CAS: 65-85-0 

#730237 25G $ 20.54 

#730238 100G $ 67.85 

#730239 25G $ 25.60 

#730240 100G $ 83.20 

#710009 100G $ 19.50 

#710011 500G $ 89.70 

#710013 1kg $ 198.00 

#764001 100G $ 13.10 

#764003 500G $ 48.80 

#710015 100G $ 35.00 

#710017 500G $ 106.00 

#710021 500G $ 21.00 

#506130 10mg $ 99.00 

#506131 25mg $ 165.00 

#705529 25G $ 30.78 

#705464 50G $ 57.02 

#705465 100G $ 92.61 

#705081 500G $ 37.43 

#705083 1kg $ 49.98 

#705085 2.5kg $ 98.00 

109 

Research Chemicals


BES - 2-[Bis(2-Hydroxyethyl)amino]Ethanesulfonic 

acid or N,N-Bis(2-Hydroxyethyl)-Taurine 

Betaine (Anhydrous) 

• MF: C 5 H 11 NO 2 

• MW: 117.15 

• CAS: 107-43-7 

D-Biotin (Vitamin H) 

Bis-Tris - Bis-(2Hydroxyethyl) 

iminotris(Hydroxymethyl)Methane 

• MF: C 8 H 19 NO 5 

• MW: 209.24 

Blue-Gal 

Boric Acid 

• MF: H 5 BO 3 

• MW: 61.83 

Bovine Albumin 

Fraction V, Crystalline 

#714118 100G $ 42.75 

#705087 50G $ 12.00 

#705089 100G $ 22.00 

#705091 1kg $ 115.00 

#764013 1G $ 33.10 

#764015 5G $ 108.80 

#764014 10G $ 214.70 

#764016 25G $ 539.00 

#730007 100G $ 36.59 

#730009 250G $ 82.44 

#730011 500G $ 137.92 

#714120 100mg $ 42.75 

#705093 500G $ 17.67 

#705095 1kg $ 29.15 

#705097 2.5kg $ 52.86 

#705511 5G $ 71.24 

Carrageenan 

Phosphate (Diabasic) #705113 500G $ 16.80 

Iota Type 

Kappa Type 

Casamino Acids - Casein, 

Acid Hydrolysate 

Cesium 

Chloride (Biotech Grade) 

Sulfate 

Cetylpyridinium Chloride 

Charcoal 

Activated Acid Washed 

#714124 100G $ 27.70 

#714126 100G $ 29.75 

#760019 100G $ 27.77 

#760021 500G $ 49.80 

#705541 50G $ 22.70 

#705135 100G $ 43.70 

#705137 500G $ 123.70 

#705139 1kg $ 199.70 

#705544 50G $ 41.05 

#705141 100G $ 78.12 

#705143 250G $ 29.72 

#705145 500G $ 52.55 

#714236 1kg $ 44.25 

#705514 10G $ 122.69 

Serum Albumin (BSA) 

#705512 25G $ 35.08 

#705463 100G $ 85.12 

Calcium 

Carbonate Anhydrous 

Chloride Dihydrate 

#705104 250G $ 288.35 

#705538 500G $ 29.19 

#705107 500G $ 22.10 

#705109 1kg $ 41.63 

110 

Citrate Tetrahydrate 

D-Gluconate 

Nitrate 

#705111 2.5kg $ 74.71 

#714122 500G $ 27.40 

#714138 500G $ 44.20 

#714140 25G $ 54.40 

> 

accelerate: yourresearch 


Chlorhexidine 

Digluconate 

4-Chloro-1-Napthol 

Cholesterol 

Choline Chloride 

Citric Acid 

• MF: C 6 H 8 O 7 

Ammonium Salt Dibasic 

Monohydrate 

Trisodium Salt Dihydrate 

Cobalt Chloride 

(hexahydrate) 

Colchicine 

Creatine Phosphate 

•C 22 H 30 CL 2 N 10 2C 6 H 12 O 7 

•MW: 897.76 

•MF: C 4 H 7 OCl 

•MW: 178.62 

•CAS: 604-44-4 

• MW 226.19 

• MW 210.14 

• MW 294.10 

• MF: C 5 H 46 O 

• MW: 386.65 

• CAS: 57-88-5 

• MF: C 5 H 14 O 

• MW: 386.65 

• CAS: 67-48-1 

#714134 100ml $ 35.00 

#705554 25G $ 40.53 

#705556 50G $ 64.16 

#705558 100G $ 40.69 

#705560 250G $ 142.75 

#714137 100G $ 16.20 

#714136 500G $ 54.70 

#705157 500G $ 20.32 

#705159 500G $ 12.81 

#705161 1kg $ 22.79 

#705163 2.5kg $ 53.76 

#714294 500G $ 31.50 

#714295 2.5kg $ 96.88 

#705165 1kg $ 16.38 

#705167 2.5kg $ 29.65 

#714142 100G $ 36.40 

#714144 100mg $ 63.40 

#705169 10G $ 56.07 

L-Cysteine HCL 

• MW:175.63 

Cytidine-5’-Triphosphate (CTP) 

Cupric Sulfate 

(pentahydrate) 

• MW: 249.69 

Cytosine 

• MW: 119.10 

Dansyl Chloride - 5- Dimethylamino-1- 

Naphthalene Sulfonyl Chloride 

dATP 2’-Deoxyadenosine-5’-Triphosphate 

dCTP 2’-Deoxycytidine-5’-Triphosphate 

DEAE Dextran 

2’-Deoxyadenosine 

Monohydrate 

2’-Deoxycytidine 

Hydrochloride 

#710025 250G $ 61.85 

#710027 500G $ 88.10 

#705173 50mg $ 31.83 

#705175 100mg $ 52.60 

#705564 500G $ 23.94 

#705566 1kg $ 39.90 

#705568 2.5kg $ 98.02 

#705177 10G $ 32.75 

#705179 25G $ 70.02 

#705181 5G $ 59.33 

#705183 10G $ 106.16 

#705191 50mg $ 71.03 

#705193 100mg $ 138.39 

#705199 50mg $ 57.60 

#705201 100mg $ 105.00 

#705578 50G $ 84.75 

#705580 100G $ 140.00 

#705582 250G $ 340.88 

#705187 5G $ 48.83 

#705189 10G $ 88.36 

#705195 5G $ 94.25 

#705197 10G $ 154.30 

biochemicals 


Cyanocobalamin 

#705171 25G $ 118.55 

#714146 5G $ 73.75 

2’-Deoxy-D-Ribose 

3-Deoxy-D-Erythro-Pentose 

#705211 5G $ 39.53 

#705213 10G $ 63.11 

finestchemicals@lowestprice 

2’-Deoxyguanosine 

Monohydrate 

2’-Deoxythymidine 

#705203 250mg $ 47.99 

#705205 1G $ 111.51 

#705227 5G $ 31.98 

#705229 25G $ 125.79 

111 


2’-Deoxyurdine 

#705239 1G $ 37.28 

Fish Testes 

#705313 5mg $ 14.40 

#705241 5G $ 149.30 

#705315 10mg $ 19.10 


Dextran 

• MF: (C 6 H 10 O 5 )n 

• MW: 10,000 

• CAS: 9004-54-0 

Sulfate 

dGTP 2’-Deoxyguanosine-5’-Triphosphate 

DICAMBA - 3,6-Dichloro-o-Anisic acid 

Diethanolamine Bis[ 2- 

Hydroxyethyl]Amine 

Diethylamine-Free Base 

DEPC (diethylpyrocarbonate) 

DMF (n’,n-dimethylformamide) 

DMSO (dimethylsulfoxide) 

DIPSO - 3-[N,N-Bis(2-Hydroxyethyl)amino]-2- 

Hydroxypropanesulfonic acid 

DTE (dl-dithioerythritol) 

• MF: C 4 H 10 O 2 S 5 

• MW: 154.25 

• CAS: 689-68-8 

(DTT) dl-dithiothreitol 

#705243 10G $ 16.90 

#705245 50G $ 40.64 

#705247 100G $ 64.58 

#705249 50G $ 58.80 

#705251 250G $ 231.53 

#705207 50mg $ 66.57 

#705209 250mg $ 298.00 

#705260 250mg $ 129.75 

#705291 1kg $ 36.00 

#705293 2.5kg $ 59.70 

#705295 950ml $ 29.00 

#714152 5G $ 18.52 

#705588 25G $ 54.34 

#705297 500ml $ 14.70 

#705299 500ml $ 23.50 

#705301 1L $ 38.70 

#730245 25G $ 35.60 

#730246 100G $ 99.50 

#705319 5G $ 38.38 

#705321 25G $ 133.16 

#705322 100G $ 400.40 

#705323 5G $ 35.00 

#705325 25G $ 98.00 

Denatured Salmon Sperm 

N-Dodecyl-D-Maltoside 

N-Dodecyl-D-Maltopyranoside 

DTTP 

(2’-Deoxythymidine-5’-triphosphate) 

EDTA Ethylenediaminetetraacetate 

Dipotassium Salt, Dihydrate 

Disodium Salt, Dihydrate 

Ferric Sodium Salt 

Free Acid 

Tetrasodium Salt, Dihydrate 

EGTA Ethyleneglycol Bis(2-Aminoehtylether)- 

N,N,N’,N’ Tetraacetic Acid 

Ethylene Glycol 1,2-Ethanediol 

#700826 10mg $ 49.95 

#700827 20mg $ 94.50 

#730056 1G $ 115.90 

#705215 50mg $ 90.62 

#705219 100mg $ 150.62 

#705327 500G $ 51.08 

#705329 1kg $ 96.29 

#705331 500G $ 42.26 

#705333 1kg $ 63.53 

#705335 2.5kg $ 158.70 

#714238 500G $ 56.25 

#714240 1kg $ 85.75 

#705337 500G $ 19.95 

#705339 1kg $ 34.50 

#705343 500G $ 22.84 

#705345 1kg $ 40.22 

#705347 2.5kg $ 69.50 

#705349 10G $ 22.40 

#705351 50G $ 53.55 

#705353 100G $ 93.45 

#705616 1L $ 16.70 

#705618 4L $ 49.50 

DNA 

Denatured Calf Thymus 

#700828 10mg $ 59.95 

#700829 20mg $ 105.00 

Ferric 

Ammonium Sulfate (Dodecahydrate) 

#705624 500G $ 40.90 

#705626 1kg $ 70.25 

112 


Gelatin (Bacteriological Grade) 

#760068 500G $ 28.25 

Chloride (Hexahydrate) 

Nitrate (Nonahydrate) 

Sulfate (Hydrate) 

#705630 500G $ 32.60 

#705632 1kg $ 55.65 

#705636 500G $ 16.42 

#705638 1kg $ 25.88 

#705640 2.5kg $ 57.20 

#705373 500G $ 34.60 

Fish (Granulated) 

D-Glucose 

Anhydrous 

#715398 15G $ 14.25 

#715399 30G $ 21.85 

#715400 60G $ 43.75 

#705401 1kg $ 17.80 

#705403 2.5kg $ 29.70 

biochemicals 

#705375 1kg $ 58.33 

Monohydrate 

#705655 1kg $ 33.34 

Ferrous Sulfate 

#705377 500G $ 33.34 

#705654 2.5kg $ 72.92 

heptahydrate 

Ficoll 400 (Molecular Biology grade) 

5-Fluorouracil 

Fluridone 

Folic Acid - Pteroylglutamic Acid 

Formamide 

D-Fructose 

Deionized 

d-(+)-Galactose 

• MF: C 4 H 3 FN 2 O 2 

• MW: 130.08 

USP Grade 

• CAS: 51-21-8 

(used for DNA / 

RNA hybridization) 

#705379 1kg $ 57.91 

#760014 100G $ 127.00 

#705383 5G $ 61.16 

#705385 10G $ 117.73 

#705387 25G $ 248.33 

#714242 100mg $ 154.00 

#705380 25G $ 30.75 

#705381 100G $ 92.25 

#705642 500ml $ 29.00 

#705644 950ml $ 39.70 

#705646 100ml $ 21.69 

#705648 500ml $ 49.95 

#705650 950ml $ 101.97 

#705389 1kg $ 16.70 

#705391 2.5kg $ 34.50 

#705395 100G $ 37.43 

#705397 250G $ 77.18 

#705399 500G $ 116.87 

L-Glutamic Acid 

L-Glutamine 

Glutathione 

Oxidized 

Reduced 

Glycerol - Glycerin 

Glycine 

Glycyl-Glycine 

• MF: C 2 H 5 NO 2 

• MW: 75.07 

• CAS: 56-40-6 

Guanidine 

Hydrochloride 

• MF: CH 5 N 2 •HCl 

• MW: 95.53 

#710031 1kg $ 26.70 

#710034 500G $ 75.29 

#710037 1kg $ 134.85 

#705405 1G $ 33.50 

#705407 5G $ 146.11 

#705409 50G $ 93.50 

#705411 250G $ 391.81 

#705417 1L $ 18.50 

#705419 4L $ 77.90 

#710038 500G $ 22.50 

#710039 1kg $ 29.50 

#710042 2kg $ 50.40 

#710041 5kg $ 97.00 

#730018 100G $ 127.00 

#730019 250G $ 247.00 

#705425 100G $ 28.35 

#705429 500G $ 92.40 

#705433 1kg $ 122.50 


113 


Thiocyanate (Isothiocyanate) 

#705435 50G $ 19.95 

Imidazole 

#705832 10G $ 27.62 


Guanine 

Guanosine 

5’-Diphosphate (GDP) 

disodium salt 

5’-Monophosphate (GMP) 

5’-Triphosphate (GTP) 

disodium salt 

HEPPS [N-(2-Hydroxyethyl) Piperazine-N’- 

(3-propane sulfonic Acid)] 

(EPPS) 

HEPES: [N-(2-Hydroxyethyl) Piperazine-N’-(2- 

ethanesulfonic Acid)] 

Free Acid 

Sodium Salt 

HEPPSO (Free Acid) N-[2- 

Hydroxyethyl]Piperazine-N’-[2-Hydroxypropanesulfonic 

Acid] 

• MF: CH 5 N 3 •CHNS 

• MW: 118.16 

#705437 250G $ 58.80 

#705439 500G $ 91.77 

#705441 25G $ 38.01 

#705443 50G $ 69.30 

#705467 50G $ 29.40 

#705469 100G $ 57.75 

#705445 250mg $ 90.30 

#705447 500mg $ 119.44 

#705449 5G $ 24.73 

#705453 50G $ 169.47 

#705455 50mg $ 34.29 

#705459 100mg $ 58.43 

#730052 25G $ 27.30 

#730054 100G $ 91.88 

#730057 50G $ 30.45 

#730059 250G $ 99.55 

#730058 1kg $ 204.00 

#730061 50G $ 27.25 

#730063 100G $ 43.95 

#730065 500G $ 197.13 

#730044 25G $ 28.87 

#730046 100G $ 95.18 

L-Isoleucine 

Isopropyl Alcohol 

a-Ketoglutaric Acid 

Kinetin 6-Furfuryl-aminopurin 

DL-Lactic Acid 

Lithium Salt 

L-(+)-Lactic Acid 

Lanthanum Acetate 

L-Leucine 

Leupeptin 

Linoleic Acid 

• MF: C 3 H 4 N 2 

• MW: 68.08 

• CAS: 288-32-4 

• MF: C 6 H 13 NO 2 

• MW: 131.18 

• CAS: 73-32-5 

• MF: C 6 H 13 NO 2 

• MW: 131.17 

• CAS: 61-90-5 

#705834 50G $ 68.25 

#705836 100G $ 115.00 

#710057 10G $ 13.65 

#710059 25G $ 34.55 

#710061 100G $ 95.75 

#705505 1L $ 20.84 

#705506 4L $ 59.85 

#705507 100G $ 33.18 

#705508 1G $ 13.25 

#705504 5G $ 44.00 

#705110 100G $ 198.00 

#705711 4L $ 45.05 

#705712 50G $ 70.46 

#705713 250G $ 279.58 

#705714 25G $ 48.02 

#705716 100G $ 160.58 

#705718 25G $ 37.42 

#705720 50G $ 85.21 

#710065 50G $ 19.24 

#710067 100G $ 30.90 

#710069 500G $ 119.00 

#705523 5mg $ 25.78 

#705524 25mg $ 104.55 

#705519 5G $ 20.42 

L-Histidine 

#710056 100G $ 57.00 

#705520 10G $ 63.00 

#710055 500G $ 154.70 

Linolenic Acid 

#705516 1G $ 46.83 

#705517 5G $ 184.91 

114 90 

Lithium Chloride 

#705526 100G $ 16.70 

#705527 500G $ 42.00

Luminol 3-Aminophthal Hydrazide 

L-Lysine 

Acetate 

• MF: C 8 H 7 N 3 O 2 

• MW: 177.16 

• CAS: 521-31-3 

• MF: C 6 H 14 N 2 O 2 

• MW: 146.19 

• CAS: 56-87-1 

#705528 10G $ 56.50 

#705534 50G $ 198.00 

#705536 100G $ 398.70 

#710071 5G $ 13.78 

#710073 25G $ 39.45 

#710075 100G $ 135.00 

#714160 100G $ 71.00 

Mercaptoethanol 

- Thioethylene Glycol, BME 

6-Mercaptopurine 

MES (free acid) 

[2-(N-Morpholino) ethanesulfonic Acid] 

L-Methionine 

#705575 100ml $ 10.92 

#705577 250ml $ 14.70 

#714162 5G $ 62.50 

#730067 100G $ 35.60 

#730069 250G $ 84.21 

#730071 500G $ 144.95 

#710079 25G $ 16.60 

biochemicals 

Magnesium 

Acetate (Tetrahydrate) 

Chloride (Hexahydrate) 

Sulfate (Heptahydrate) 

Malachite Green Oxalate 

Maleic Acid Hydrazide 

3,6 - Dihydroxypyridazine 

DL-Malic acid 

Manganese Sulfate 

Monohydrate 

D-Mannitol 

• MW: 214.45 

• MW: 203.30 

• MW: 246.48 

#705535 500G $ 18.11 

#705537 1kg $ 32.03 

#705539 2.5kg $ 74.28 

#705543 500G $ 23.10 

#705545 1kg $ 39.90 

#705553 500G $ 16.15 

#705555 1kg $ 28.35 

#705557 2.5kg $ 61.00 

#705733 100G $ 14.97 

#705734 250G $ 29.40 

#714252 25G $ 17.60 

#714254 100G $ 35.75 

#705305 2.5kg $ 26.99 

#705559 1kg $ 26.83 

#705563 2.5kg $ 51.66 

#705565 500G $ 23.05 

#705567 1kg $ 40.58 

4-Methylumbelliferyl 

Phosphate (MUP) 

Metribuzin 4-Amino-6-Tert-Butyl-4,5-Dihydro-3-Methylthio-1,2,4-Triazin-5-one 

Molybdic Acid 

NH4 + (Tetrahydrate) 

• MF: (NH4) 6 Mo 7 O 24 • 4H 2 O 

Sodium Salt 

• MF: Na 2 MoO 4 • 2H 2 O 

MOPS [3-(N-Morpholino) propanesulfonic 

Acid] 

Free Acid 

Sodium Salt 

MOPSO- 3-Morpholino-2-Hydroxypropane 

sulfonic Acid 

Free Acid 

#710081 100G $ 40.65 

#715131 1G $ 79.75 

#706300 1G $ 41.95 

#705747 250G $ 28.30 

#705748 500G $ 51.40 

#705755 5G $ 17.50 

#705752 250G $ 39.75 

#705754 500G $ 73.08 

#730073 100G $ 29.40 

#730075 250G $ 65.10 

#730077 500G $ 110.99 

#730078 25G $ 24.00 

#730080 100G $ 36.75 

#730082 250G $ 78.75 

#730084 25G $ 26.05 

#730086 1kg $ 69.70 


D-Mannose 

#705572 25G $ 23.10 

#705571 100G $ 55.71 

#705573 500G $ 198.70 


115 


Sodium Salt 

#730088 25G $ 24.50 

Oxalic Acid Dihydrate 

#705787 500G $ 26.25 


MYO-Inositol i-Inositol, meso-Inositol 

NADH (Reduced) 

NADP 

1,5-Naphthalenedisulfonate salt 

Naphthol 

AS-MX Phosphate 

Phosphate (Sodium Salt) 

Naphthylacetamide 2 (1-Naphthyl) 

Acetamide 

Nicotinamide 

Nocotinamide Adenine 

Dinucleotide (NAD) 

Nicotinic Acid 

Ninhydrin 

• MF: C 6 H 12 O 6 

• MW: 180.2 

• CAS: 87-89-8 

• MF: C 21 H 26 N 7 O 17 P 3 Na 2 • 3H 2 O 

• MW: 841.40 

• CAS: 53-59-8 

• MF: C 21 H 27 O 14 N 7 P 2 • 3H 2 O 

• MW: 717.50 

• MF: C 6 H 5 NO 2 

• MW: 123.11 

Nitrofurantoin N-(5-Nitro-2-Furfurylidene)-1-Aminohydantoin 

#730090 100G $ 44.53 

#714212 50G $ 25.95 

#714213 100G $ 39.00 

#714214 250G $ 78.00 

#705581 1G $ 31.50 

#705583 5G $ 133.98 

#705585 500mg $ 76.65 

#705587 1G $ 121.80 

#705589 5G $ 483.00 

#715081 5G $ 74.55 

#715083 25G $ 341.25 

#705766 1G $ 48.51 

#705767 1G $ 11.71 

#705768 5G $ 35.70 

#705597 100mg $ 36.90 

#705599 500mg $ 176.40 

#714168 250G $ 24.60 

#705591 1G $ 22.05 

#705593 5G $ 82.96 

#705595 10G $ 139.10 

#714171 50G $ 19.50 

#714170 250G $ 38.70 

#705605 25G $ 21.05 

#705607 100G $ 68.78 

#714172 25G $ 32.50 

Paclobutrazol N-Dimethylaminosuccinamic 

Acid 

Paraplast 

D-Pantothenic Acid 

(CalciumSalt), Vitamin B 

Peptone Water 

Phenazine Methosulfate 

L-Phenylalanine 

Phenol 

Crystalline 

Red 

Red (Sodium Salt) 

Phenylacetic Acid (PAA) 

O-Phenylenediamine (OPD) 

Phenylmethyl Sulfonyl 

Fluoride (PMSF) 

Phloroglucinol 

• MF: C 6 H 5 OH 

• MW: 94.11 

• CAS: 108-95-2 

• MF: C 19 H 14 O 5 S 

• MW: 354.38 

• MW: 376.36 

Picloram 4-Amino-3,5,6- 

-tissue embedding media 

trichloropicolinic Acid 

#705788 1kg $ 42.00 

#714260 25G $ 15.25 

#714262 100G $ 49.70 

#506115 1kg $ 36.50 

#714176 100G $ 26.25 

#765199 100G $ 17.75 

#765210 500G $ 36.95 

#705804 10G $ 77.70 

#710085 25G $ 12.00 

#710086 100G $ 37.00 

#705806 100G $ 30.40 

#750808 250G $ 47.70 

#705807 500G $ 84.00 

#705810 1kg $ 134.40 

#705814 25G $ 19.85 

#705815 50G $ 30.08 

#705816 25G $ 23.28 

#705817 50G $ 34.97 

#714182 250G $ 22.90 

#715075 25G $ 12.50 

#705619 5G $ 28.77 

#705621 25G $ 96.60 

#714264 25G $ 41.00 

#714266 100G $ 143.65 

#714268 5G $ 18.75 

#714269 10G $ 29.25 

116 92 


Pipes Sodium Salt Piperazine-1,4-bis (2- 

ethanesulfonic Acid) 

Polyethylene Glycol 

PEG 1000 

PEG 4000 

PEG 8000 

PVP (Polyvinylpyrrolidone) 

POPSO Piperazine-N,N’-Bis(2-Hydroxypropanesulfonic 

Acid) 

Disodium Salt 

Free Acid 

• MW: 342.35 

• MF: (C 6 H 9 NO) n 

• MW: 760.58 

#730099 100G $ 35.70 

#730101 250G $ 80.64 

#714220 500ml $ 19.00 

#714222 4L $ 73.00 

#714224 500G $ 33.00 

#714226 2.5kg $ 68.00 

#705631 500G $ 19.80 

#705633 1kg $ 29.81 

#705635 2.5kg $ 47.99 

#705842 500G $ 39.90 

#705843 1kg $ 71.82 

#730334 25G $ 19.30 

#730335 100G $ 56.00 

#730336 25G $ 17.00 

#730337 100G $ 49.30 

Hydroxide 

Iodide 

Nitrate 

Permanganate 

Persulfate 

Phosphate Dibasic Anhydrous 

Phosphate Monobasic 

(Anhydrous) 

#705653 500G $ 13.65 

#705656 1kg $ 18.90 

#705657 100G $ 17.85 

#705659 500G $ 69.30 

#705661 2.5kg $ 255.94 

#705663 1kg $ 45.65 

#705665 2.5kg $ 82.32 

#705852 500G $ 19.50 

#705667 500G $ 38.64 

#705669 2.5kg $ 184.70 

#705672 500G $ 30.45 

#705671 1kg $ 53.95 

#705673 2.5kg $ 114.70 

#705675 500G $ 20.75 

#705676 1kg $ 37.50 

#705677 2.5kg $ 63.58 

biochemicals 


Potassium 

Acetate 

Cacodylate 

Carbonate (Anhydrous) 

Chloride 

Ferricyanide 

#705639 1kg $ 48.98 

#705641 2.5kg $ 109.52 

#730142 2x25G $ 437.50 

#705850 1kg $ 35.28 

#705851 2.5kg $ 69.59 

#705643 500G $ 18.90 

#705645 1kg $ 29.40 

#705647 2.5kg $ 64.05 

#705649 500G $ 23.68 


L-Proline 

Sorbate (Dibasic) 

Propylene Glycol 

• MF: C 5 H 8 NO 2 

• MW: 115.13 

• MF: C 3 H 8 O 2 

• MW: 76.10 

Pyridoxal-5-phosphate 

• MF: C 8 H 10 NO 6 P 

• MW: 76.10 

Pyridoxine HCL - Vitamin B6 

• MW: 205.64 

Pyruvic Acid Sodium Salt 

• MF: CH 3 COCO 2Na 

• MW: 110.05 

#705853 500G $ 21.84 

#710093 25G $ 14.70 

#710095 100G $ 43.05 

#705870 1L $ 19.48 

#705872 4L $ 47.04 

#705683 5G $ 29.61 

#705685 25G $ 123.69 

#705682 25G $ 20.88 

#705681 100G $ 46.50 

#705876 100G $ 39.48 

#705877 250G $ 89.25 

117 



Raffinose 

Riboflavin 

Vitamin B2 

D-Ribose 

5’-Phosphate 

Rubidium Chloride 

Salicylic Acid 

Salmon X-Gluca 6-Chloro-3-Indolyl-B-D- 

Glycuronide CyclohexylammoniumSalt 

L-Serine -Hydroxyalanine 

Silver Nitrate 

Sodium 

• MF: C 18 H 32 O 16 *5H 2 O 

• MW: 594.53 

• MW: 376.37 

• MW: 515.36 

• MF: C 5 H 10 O 5 

• MW: 150.13 

Acetate (Anhydrous) 

Acetate (Trihydrate) 

Azide 

• MW: 82.03 

• MW: 136.08 

• MW: 65.02 

Bicarbonate 

• MW: 84.01 

#714186 25G $ 50.00 

#764021 250G $ 51.20 

#764023 500G $ 83.50 

#764017 25G $ 20.50 

#764019 100G $ 63.50 

#705687 50G $ 36.75 

#705689 100G $ 69.30 

#705691 25G $ 46.78 

#705693 50G $ 81.85 

#714190 100G $ 22.80 

#714194 10mg $ 71.11 

#710101 5G $ 4.08 

#710103 25G $ 23.40 

#710105 100G $ 59.70 

#705695 25G $ 45.20 

#705697 100G $ 147.00 

#705701 500G $ 16.63 

#705703 1kg $ 26.73 

#705705 2.5kg $ 63.48 

#705715 500G $ 18.70 

#705717 1kg $ 22.10 

#705719 2.5kg $ 44.15 

#705721 250G $ 33.60 

#705723 500G $ 67.20 

#705725 1kg $ 17.33 

#705727 2.5kg $ 31.50 

D-Sorbitol 

Borate (Decahydrate) 

Carbonate (Anhydrous) 

• MW:105.99 

Chloride 

• MW: 58.44 

Hydroxide 

• MW: 40.00 

Iodide 

• MW: 149.89 

Metabisulfate 

• MW: 190.11 

Phosphate (Dibasic) 

• MW: 268.07 

Phosphate Monobasic 

• MW: 137.99 

Sulfate Anhydrous • MW:142.04 

Sulfite Anhydrous 

• MW: 126.04 

Thiosulfate Pentahydrate 

• MW: 248.19 

Spermidine N-(3-Aminopropyl)-1,4- 

Butanediamine 

Streptavidin 

• MW: 182.17 

#705884 1kg $ 16.24 

#705735 500G $ 16.44 

#705737 1kg $ 29.90 

#705738 2.5kg $ 64.00 

#705744 500G $ 18.90 

#705745 1kg $ 31.50 

#705746 5kg $ 61.69 

#705758 500G $ 19.90 

#705761 1kg $ 29.40 

#705763 5kg $ 144.45 

#705769 250G $ 32.34 

#705770 500G $ 57.08 

#705773 500G $ 17.40 

#705774 1kg $ 23.52 

#705777 5kg $ 49.35 

#705783 500G $ 27.30 

#705784 1kg $ 44.10 

#705904 500G $ 24.94 

#705906 1kg $ 42.32 

#705795 1kg $ 25.29 

#705909 500G $ 12.11 

#705910 1kg $ 23.42 

#705912 500G $ 17.60 

#714196 500G $ 21.70 

#714197 1kg $ 39.70 

#714272 1G $ 18.75 

#714270 5G $ 70.00 

#705801 1mg $ 42.84 

118 

#705803 5mg $ 172.99 


Succinic Acid 

Sucrose 

• MF: C 12 H 22 O 11 

• MW: 336.40 

5-Sulfosalicylic Acid 

TAPS N-Tris(Hydroxymethyl) methyl-2-aminopropanesulfonic 

Acid 

Free Acid 

#705918 500G $ 15.26 

#705820 500G $ 11.50 

#705821 1kg $ 17.50 

#705921 250G $ 31.98 

#705922 500G $ 57.91 

#730344 25G $ 29.24 

#730338 100G $ 110.00 

(TDZ) Thidiazuron Phenyl-N’-(1,2,3-Thiadiazol-5-yl)Urea 

Thimerosal 

L-Threonine 

• MF: C 9 H 9 H 9 NaO 2 S 

• MW: 404.8 

#714276 100mg $ 80.25 

#714275 250mg $ 179.50 

#714274 500mg $ 320.25 

#714200 10G $ 56.25 

#714199 25G $ 93.60 

#710109 10G $ 15.50 

#710111 25G $ 36.40 

biochemicals 

biochemicals 

Sodium Salt 

TEMED - N,N,N’,N’-Tetramethylethylene-Diamine 

TES (sodium salt) - N-Tris[Hydroxymethyl]M 

ethyl-2-Aminoethanesulfonic Acid 

TTAB tetradecyl trimethyl ammonium Bromide 

• MF: C 17 H 38 NBr 

Tetramethyl Ammonium Chloride 

3,3’,5,5’-Tetramethyl 

Benzidine 

1H-Tetrazole 

• MW: 336.40 

Thiamine HCL - Vitamin B1 

Thiazoyl Blue 

Tetrazolium Bromide (MTT) 

• MF: C 18 H 16 N 5 SBr 

• MW: 414.33 

• CAS: 298-93-1 

#730339 25G $ 19.50 

#730340 100G $ 43.50 

#705924 25ml $ 14.70 

#705818 50ml $ 22.32 

#705819 100ml $ 42.11 

#730341 25G $ 27.90 

#730342 100G $ 74.50 

#705859 100G $ 16.12 

#705860 500G $ 51.19 

#705861 1kg $ 76.00 

#705824 100G $ 17.01 

#705825 500G $ 65.68 

#715069 250mg $ 12.93 

#715071 1G $ 24.97 

#715073 5G $ 89.04 

#705927 25G $ 235.20 

#714198 10G $ 19.50 

#705762 500mg $ 23.10 

#705764 1G $ 32.55 

#705765 5G $ 119.70 

Trichloroacetic Acid (TCA) 

Tricine Buffer - N-Tris[Hydroxymethyl]Methylglycine 

Trifluralin 

Trimethoprim 

Trimethoprim Lactate 

Tris 

Acetate 

Base 

• ACS Grade 

• MW: 163.39 

• 80% Solution 

Biotechnology Grade 

Carbonate 

Hydrochloride 

Ultra Pure Grade 

#705994 125G $ 36.70 

#705995 500G $ 75.58 

#705991 500ml $ 69.85 

#705992 1L $ 115.00 

#730181 100G $ 23.70 

#730183 250G $ 51.45 

#730185 500G $ 75.55 

#714204 250mg $ 187.50 

#714206 5G $ 50.00 

#714208 250mg $ 118.75 

#730187 100G $ 34.39 

#730192 500G $ 169.74 

#730205 1kg $ 49.85 

#730219 500G $ 44.70 

#705964 500G $ 28.01 

#705966 1kg $ 50.51 

#705957 100G $ 27.75 

#730191 500G $ 51.45 

#730193 1kg $ 68.14 

#730204 500G $ 35.00 

#730201 1kg $ 54.95 


119 



Tryptone 

L-Tryptophan 

• MW: 204.23 

TTC 2,3,5-Triphenyl-2H-Tetrazolium chloride 

L-Tyrosine L-3-(4-Hydroxyphenyl) Alanine 

• MW: 181.19 

Uracil 

• MW: 112.09 

Urea 

Ultra Pure Grade 

USP Grade 

Uridine- ultrapure grade 

#760077 100G $ 16.69 

#760079 500G $ 39.70 

#760085 2kg $ 137.50 

#710125 25G $ 22.05 

#710127 100G $ 70.85 

#705980 25G $ 46.20 

#705981 100G $ 123.85 

#710133 25G $ 12.60 

#710131 100G $ 43.30 

#705833 250G $ 27.07 

#705835 500G $ 50.30 

#705846 500G $ 25.93 

#705847 1kg $ 42.21 

#705848 2.5kg $ 77.18 

#705985 1kg $ 14.90 

#705986 5kg $ 78.00 

#705863 25G $ 35.65 

Zeatin 

Zinc Sulfate 

• USP Grade 

• MW: 287.59 

#706200 50mg $ 29.70 

#706202 100mg $ 98.70 

#706204 250mg $ 198.45 

#706205 500G $ 22.07 

#706206 2.5kg $ 83.95 

• MW: 224.20 

#705864 50G $ 72.35 

5’Triphosphate (UTP) 

#705854 100mg $ 32.55 

#705855 250mg $ 40.74 

L-Valine 

• MW: 117.15 

#710141 25G $ 14.60 

#710143 100G $ 43.05 

> 


120 

107 


iomedia 

8 

Animal Media 

Microbiological Media 

Plant Media 

> 

accelerate: cellcultureresearch

iomedia 

Animal 

Animal Cell Culture Media biomedia 

122 

DMEM 

• Dulbecco’s Modified Eagle’s Medium-a modification of Basal Medium 

Eagle (BME). 

• Original formulation of DMEM contained 1000mg/L of glucose, but 

since has been raised in some modifications to 4500mg/L. 

• Hams F-12 nutrient mixture (1:1) was created to determine the media 

requirements for individual cell types. 

Hams F-12 (1:1) with HEPES 

• Contains: L-Glutamine, 

Pyridoxine Hydrochloride & 

15mM HEPES buffer. 

#785058 

#785059 

500ml Lq. 

1L Lq. 

$ 8.15 

$ 11.82 

#785060 1L powder $ 6.42 

#785061 10L powder $ 23.49 

#785062 50L powder $ 97.89 

Hams F-12 (1:1) 

• See above for info 

• Without HEPES 

#785063 500ml Lq. $ 7.91 

#785064 1L Lq. $ 11.34 

#785065 1L powder $ 5.91 

#785066 10L powder $ 18.31 

#785067 50L powder $ 76.35 

Hams F-12 (1:1) 


• Without Phenol Red 

#785068 500ml Lq. $ 8.13 

#785069 1L Lq. $ 11.79 

High Glucose with L-Glutamine 

• Contains: Pyridoxine 

Hydrochloride supplement 

#785010 500ml Lq. $ 8.60 

with 25mM HEPES buffer #785011 1L Lq. $ 12.72 

• Contains Phenol Red 

#785012 1L powder $ 7.86 

#785013 10L powder $ 29.97 

#785014 50L powder $ 124.87 

High Glucose with L-Glutamine (Modification 1) 



• Does not include 

Sodium Pyruvate, Myo-Inositol 

#785025 

#785026 

500ml Lq. 

1L Lq. 

$ 8.15 

$ 11.82 


• See above for info. 


#785020 #785025 500ml Lq. $ 8.15 8.57 

#785021 #785026 1L Lq. $ 11.82 12.65 

#785022 1L powder $ 7.86 

#785023 10L powder $ 29.97 

#785024 50L powder $ 124.88 




#785005 #785025 500ml Lq. $ 8.13 8.15 

• Does not include 

#785006 #785026 1L Lq. $ 11.30 11.82 

Sodium Pyruvate or 

#785007 1L powder $ 6.49 

Sodium Biocarbonate 

• With Pyridoxine Hydrochloride 

#785008 10L powder $ 23.96 

#785009 50L powder $ 99.83 



• Without Sodium Phophate 

#785027 500ml Lq. $ 8.15 

#785028 1L Lq. $ 11.76 



Hydrochloride supplement & 

#785015 #785025 500ml Lq. $ 8.15 8.21 

110mg/L Sodium Pyruvate #785016 #785026 1L Lq. $ 11.82 11.96 

• With Sodium Pyruvate #785017 1L powder $ 6.62 

#785018 10L powder $ 24.32 

#785019 50L powder $ 105.75

High Glucose without L-Glutamine (Modification 6) 



25mM HEPES buffer 

#785038 

#785039 

500ml Lq. 

1L Lq. 

$ 8.49 

$ 12.00 




• Does not contain 

Sodium Pyruvate Phenol Red 

#785034 

#785035 

500ml Lq. 

1L Lq. 

$ 8.00 

$ 11.52 

High Glucose w/o L-Glutamine 


• With Pyridoxine Hydrochloride 

#785029 500ml Lq. $ 8.04 

#785030 1L Lq. $ 11.60 

#785031 1L powder $ 6.43 

#785033 10L powder $ 23.57 

#785032 50L powder $ 98.18 



Hydrochloride & Sodium 

Pyruvate supplement 

#785036 

#785037 

500ml Lq. 

1L Lq. 

$ 8.10 

$ 11.76 

Low Glucose with L-Glutamine 



25mM HEPES 

#785045 

#785046 

500ml Lq. 

1L Lq. 

$ 8.61 

$ 12.75 

Low Glucose with L-Glutamine (Modification 1) 



110mg/L Sodium Pyruvate 

#785040 

#785041 

500ml Lq. 

1L Lq. 

$ 8.15 

$ 11.82 

#785042 1L powder $ 5.57 

#785043 10L powder $ 21.75 

#785044 50L powder $ 90.96 

Low Glucose without L-Glutamine (Modification 2) 


Hydrochloride supplement, 

25mM HEPES buffer & 110mg/L 

Sodium Pyruvate 

#785056 

#785057 

500ml Lq. 

1L Lq. 

$ 8.51 

$ 12.03 

Low Glucose without L-Glutamine without Phenol Red 



& 110mg/L Sodium Pyruvate 

#785054 

#785055 

500ml Lq. 

1L Lq. 

$ 8.01 

$ 11.55 

Low Glucose w/o L-Glutamine w/Sodium Pyruvate 


• With Sodium Pyruvate 

#785052 500ml Lq. $ 8.04 

#785053 1L Lq. $ 11.61 

No Glucose with L-Glutamine 



• Does not contain Sodium 

Pyruvate or 

#785047 

#785048 

#785049 

500ml Lq. 

1L Lq. 

1L powder 

$ 8.04 

$ 11.61 

$ 5.81 

Sodium Bicarbonate 

#785050 10L powder 

#785051 50L powder 

$ 18.84 

$ 75.38 

Iscove’s with L-Glutamine 

• Contains: 25mM HEPES 

• Has been shown to support 

murine B lymphocytes, 

#785105 

#785106 

500ml Lq. 

1L Lq. 

$ 8.73 

$ 14.20 

hemopoietic tissue from bone marrow, B cells stimulated with 

lipopolysaccharide, T lymphocytes & a variety of hybrid cells. 

Iscove’s with L-Glutamine without Phenol Red 

• Contains: 25mM HEPES 


#785100 500ml Lq. $ 8.68 

Sodium Bicarbonate or #785101 1L Lq. $ 12.30 

Phenol Red 

#785102 1L powder $ 5.36 

#785103 10L powder $ 28.99 

#785104 50L powder $ 120.75 

biomedia Animal Cell Culture Media 

123

Fetal Calf Bovine Serum 

• Please inquire for availability & specifications. 

Heat Inactivated 

#760254 500ml $ 240.00 

F-10 with L-Glutamine 



#785079 500ml Lq. $ 7.70 

#785080 1L Lq. $ 10.90 

Animal Cell Culture Media biomedia 

Standard Grade #760252 500ml $ 240.00 

Premium Grade 

#760250 500ml $ 397.50 

Grace’s Media 

• When supplemented with the proper components, this medium has 

been shown to support a variety of insect cells, including several 

lepidopteran as well as some dipterans. 

With L-Glutamine 

#785071 500ml Lq. $ 11.62 

#785072 1L Lq. $ 18.03 

Without L-Methionine #785073 500ml Lq. $ 11.82 

#785074 1L Lq. $ 19.89 

Supplement (Hink’s) 

#785075 500ml Lq. $ 12.18 

#785076 1L Lq. $ 19.30 

#785077 1L powder $ 11.80 

#785078 10L powder $ 93.86 


• Contains: 25mM HEPES buffer 

F-12 with L-Alanine 

• Contains: Dipeptide L-Alanine; 

L-Glutamine substituted on a 

molar equivalent basis 

for L-Glutamine 






#785081 1L powder $ 5.91 

#785082 10L powder $ 18.24 

#785083 500ml Lq. $ 8.19 

#785084 1L Lq. $ 12.00 

#785091 500ml Lq. $ 8.16 

#785092 1L Lq. $ 11.84 

#785085 500ml Lq. $ 7.81 

#785086 1L Lq. $ 11.16 

#785087 1L powder $ 5.90 

#785088 10L powder $ 18.24 

#785089 500ml Lq. $ 7.82 

Ham’s Nutrient Mix 

• Mixtures F-10 & F-12 were both originally developed to support the 

growth of a variety of cells, particularly the Chinese Hamster Ovary 

(CHO) cells, with or without serum supplementation. 

• Ham’s F-10 has been used in the culture of a wide variety of cells of 

human & animal origin. 

• Ham’s F-12 is preferred for the culture of rodent cells & cloning medium 

for myeloma & hybridoma cells. 

F-12 Kaighn’s Modification 

• Contains: L-Glutamine 

> 

accelerate: cellculture 

#785090 1L Lq. $ 11.16 

#785093 500ml Lq. $ 8.16 

#785094 1L Lq. $ 11.85 

124 


Human Serum 

• Human plasma is collected from healthy male donors at FDA licensed 

facilities located within the U.S. 

• Individual units are converted to serum, pooled, filtered, bottled & cell 

culture tested for mycoplasma, endotoxin & sterility. 

• Individual units are tested & found negative for all required viral 

markers via FDA approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1Ag 

or Hiv-1 NAT, ALT, & syphilis). 

Normal 

#760264 100ml $ 89.00 

Heat Inactivated 

#760265 500ml $ 98.00 

• At 56°C for 30min 

IPL-41 Insect Medium 

• Designed to support 

the growth & maintenance of 

#785095 500ml Lq. $ 13.45 

cell lines descending from #785096 1L Lq. $ 21.27 

lepidopteran species & the #785097 1L powder $ 14.15 

propagation of viruses within 

these cells, & for large scale 

#785098 10L powder $ 120.48 

production of baculovirus. 

• Contains: L-Glutamine & 

Calcium Chloride 

#785099 50L powder $ 462.50 

• Does not contain Tryptose Phosphate Broth 

With L-Glutamine #785107 500ml Lq. $ 8.25 

#785108 1L Lq. $ 12.00 

#785109 1L powder $ 6.68 

#785110 10L powder $ 26.00 

#785111 50L powder $ 108.30 

Without L-Glutamine #785112 500ml Lq. $ 8.24 

#785113 1L Lq. $ 12.50 

Mccoy’s 5a Media 

#785114 1L powder $ 7.75 

#785115 10L powder $ 25.95 

#785116 50L powder $ 108.15 

• For use in work with human myeloblasts. 

• Does not contain Sodium Bicarbonate 

With L-Glutamine #785117 500ml Lq. $ 8.73 

#785118 1L Lq. $ 12.98 

#785119 1L powder $ 7.36 

#785120 10L powder $ 28.99 

#785121 50L powder $ 122.25 

biomedia Animal Cell Culture 

Leibovitz’s l-15 Medium 

• Formulated to support the growth & proliferation of several cell lines 

in CO 2 - free systems without a sodium bicarbonate supplement. 

• Buffered by its salts, free base amino acids & galactose (substitutes 

for glucose). 

• Can be used under conditions free of gaseous exchange with 

the atmosphere. 

Without L-Glutamine 

#785122 500ml Lq. $ 8.75 

#785123 1L Lq. $ 12.97 

#785124 1L powder $ 7.35 

#785125 10L powder $ 28.99 

#785126 50L powder $ 120.75 

> 

accelerate: animalcellresearch 

125

Animal Cell Culture biomedia 

MCDB 131 Medium 

• Developed for the culture of 

specific cell types without a 

serum supplement. 

#785127 

#785128 

500ml Lq. 

1L Lq. 

$ 7.97 

$ 11.50 

• Supplemented with growth factors, hormones, trace elements or low 

levels of dialyzed fetal bovine serum protein (FBSP). 

Medium 199 

• For growth of explanted tissue. 

• Long term cultivation of cells requires a serum supplement. 

• Used in virology, vaccine production, & the culture of many 

non-transformed cells. 

With Earle’s Salts and L-Glutamine 

#785135 500ml Lq. $ 9.95 

#785137 1L powder $ 5.83 

#785138 10L powder $ 13.37 

With Earle’s Salts and HEPES 



#785133 500ml Lq. $ 9.95 

#785134 1L Lq. $ 10.98 

With Hank’s Salt and L-Glutamine 

#785139 500ml Lq. $ 8.16 

#785140 1L Lq. $ 12.00 

With Hank’s Salt and HEPES 



#785141 500ml Lq. $ 8.20 

#785142 1L Lq. $ 11.93 

Microplate Replicator 

#054048 48 well $ 285.70 

biomedia 

Microbiological Media 

bioWorld formulates a large selection of 

microbiological media and can custom produce other 

formulations. For a full line of microbiomedia and media 

ingredients, please visit www.microbiomedia.com 

& www.bio-world.com. 

Agar 

• Bacteriological Grade 

• CAS: 9002-18-0 

• Melting Point: 84-88ºC 

• Gel Point (1.5%): 34-38ºC 

• Gel Strength: 550-950g/cm 2 

• Pass through 60 mesh:> 90% 

LB Broth, Lennox 

• Formulated for the propagation 

and maintenance of E. coli for 

molecular biology. 

• Contains: Bacto Tryptone, 

Yeast Extract, Sodium Chloride 

• pH 7.0 + 0.2 

- 

• Medium salt concentration 

Replica Platters & Valvet Squares 

To replicate bacterial or yeast colonies 

on a plate 

#760007 100g $ 26.40 

#760008 500g $ 69.70 

#760155 500g $ 29.70 

#760156 1kg $ 56.50 

#760157 2kg $ 107.50 

#270500 100mm $ 49.70 

#270550 150mm $ 98.70 

126 

#054096 96 well $ 386.70 

Stainless steal prongs 

- 

See Valvet & Valvet Squares (page 127) 

- 


LB Broth, Miller 

• Formulated for the propagation 

and maintenance of E. coli for 

- 

molecular biology. 

• Contains: Bacto Trytone, Bacto 

Yeast Extract, Sodium Chloride 

• pH 7.3 + 0.2 

• Higher salt concentration 

Super Broth 

• An extremely rich medium for 

obtaining high yields of bacteria 

- 

and lambdabacteriophage in liquid lysates. 

Yeast Extract 

#760151 500g $ 29.70 

#760154 1kg $ 54.00 

#760153 2kg $ 99.70 

#760152 500g $ 37.60 

• Water soluble portion of 

#760091 100g $ 19.50 

autolyzed yeast and source of 

#760093 500g $ 44.25 

vitamin B complex 

• A high vitamin content - provides 

excellent growth factors for most 

#760095 1kg $ 72.35 

microorganisms. 

- 

• Usually added at a concentration of 3-5g/L 

Yeast Nitrogen Base 

• For classification of yeast based on carbon assimilation. 

• Carbon source: Dextrose 

• Nitogen source: Ammonium Sulfate 

• Missing essential amino acid Histidine 

Histidine Dropout 

• Missing Histidine 

Leucine Dropout 

• Missing Leucine 

Tryptophane Dropout 

• Missing Trytophane 

Uracil Dropout 

• Missing Uracil 

#760355 1L $ 74.52 

#760356 1L $ 74.52 

#760357 1L $ 74.52 

#760358 1L $ 74.52 

UniLawn Inoculation Turntables 

2 in 1 

100mm, 150mm 

UniLawn 100mm 

Velvet Squares 10/pk 

6/pk 

#270100 100mm $ 88.90 

#270500 100mm $ 49.70 

#270506 6 x 6” $ 15.95 

#270510 9 x 9” $ 22.95 

biomedia Microbiology Media 

Yeast Nitrogen Base without Carbon Source 

• Ammonium sulfate is the sole 

nitrogen source. 

• Requires the addition of a carbon 

source. 

#760099 100g $ 59.50 

Velveteen Squares 10/pk 

6/pk 

#270507 6 x 6” $ 12.97 

#270511 9 x 9” $ 20.50 

127 


Plant Tissue & Cell Culture Media biomedia 

biomedia 

Plant Tissue & Cell Culture Media 

BioWorld provides a complete line of plant tissue culture media 

for transformation, regeneration and propagation of plants at 

very competitive prices. For the full line of plant tissue culture 

media, please visit www.plantmedia.com 

Custom formulations are available. 

MS Medium, Modification 1 plus Vitamins 

• Contains: microelements, 1/2 

macroelements and vitamins 

• Sucrose, cytokines and auxins 

should be added as needed 

• Storage: 2-8º C 

#765087 10 x 1L $ 16.70 

#765088 1 x 10L $ 14.80 

#765089 1x 25L $ 29.80 


• Contains: microelemints, 3/4 macro 

elements and vitamins 




For all plant tissue culture and plant 

transformation reagents 

visit www.plantmedia.com 

#765093 10 x 1L $ 16.70 

#765094 1 x 10L $ 14.80 

#765095 1x 25L $ 29.80 


• Contains: microelements, macro 

elements with 1/2 concentration of 

NH 4 

, NO 3 

and KNO 3 

, 

and vitamins 


should be added as needed. 


#765099 10 x 1L $ 16.70 

#765100 1 x 10L $ 14.80 

#765101 1x 25L $ 29.80 

MS Medium with Van Der Salm Modification 

• Contains: micro elements and 

macroelements with FeNaEDTA 

replaced by FeEDDHA 

• Sucrose, cytokines, vitamins and 

auxins should be added as needed 


MS Medium with Finer and Nagasawa Modification 

• Contains: microelements, macro 

elements with 1/2 concentration of 

NH 4 

NO 3 

and 1.6 x 

concentration of KNO 3 

• Sucrose, cytokines, vitamins and 

auxins should be added as needed 


#765111 10 x 1L $ 16.70 

#765112 1 x 10L $ 14.80 

#765113 1x 25L $ 29.80 

#765108 10 x 1L $ 15.60 

#765109 1 x 10L $ 13.70 

#765110 1x 25L $ 26.80 

128 

• transformation • regeneration • propagation

MS Medium plus Gamborg’s B5 Vitamins 

• Contains: Macro and Micro 

#765075 10x1L $ 16.70 

elements, vitamins 

• Sucrose, cytokines and auxins #765076 1x10L $ 15.20 



#765077 1x25L $ 29.80 

MS Medium plus MES Buffer 


#765081 10x1L $ 16.70 




• MES is non toxic for plant tissue 

and cells. 

#765083 1x25L $ 29.80 

• MES is an excellent buffer for plant media because of a 

high buffer capacity and its ph range of 5.5-6.7. 


MS Medium plus Nitsch Vitamins 


#765078 10x1L $ 16.70 





#765080 1x25L $ 29.80 

MS Medium with Shoot Multiplication Medium B 

New 

• Contains: micro elements and 

#765120 10x1L $ 16.70 

macro elements with FeNaEDTA 

replaced by FeEDDHA 

#765121 1x10L $ 14.80 




#765122 1x25L $ 29.80 

BASTA® (Glufosinate-ammonium) 

New 

• Selective herbicide for plant 

transformation studies using 

nucleatide sequence of the 

#714229 100ml $ 37.50 

phosphinothricin N-acetyltransferase gene from Streptomyces virido 

chromogenes, “Bar gene”. Acts by inhibiting glutamine snthetase and 

thus blocks all nitrogen assimilation into the plant. 10% solution. 

• Also known as gluphosinate 

FungiGone 

New 

• FuniGone is bioWorld’s proprietary 

#765174 100ml $ 37.50 

reagent formulation 

• This product will drastically reduce 

the growth of fungi in your tissue 

#765175 500ml $ 69.00 

culture applications, without affecting the plant tissue 

• Supplied as 100X concentrate 

• In vitro plant tissue culture-tested. 

biomedia Plant Tissue and Cell Culture Media 

plantMedia.com 

129 



9 

Affinity Purification 

Custom Peptide Synthesis 

Polycolonal Antibody Production 

Monoclonal Antibody Production 

1-D & 2-D Gel Analysis 

Recombinant Technologies 

(visit www.bio-WORLD.com 

for more custom lab services) 

> 




Antibody Purification with Protein A 

• bioWorld can purify your 

monoclonal and polyclonal 

IgG from ascites and serum. Ideal for subclasses of IgG from human, 

mouse, rabbit and sheep. 

• bioWorld can purify your 

monoclonal and polyclonal 

#501091 service inquire 

Antibody Purification with Protein G 

IgG from ascites, serum and cell culture supernatants. Ideal for 

IgG subclasses which bind poorly to protein-A such as bovine, goat and 

rat. 

Antigen Affinity Purification 

• bioWorld can perform custom 

coupling of an antigen or 


peptide of your choice to an affinity matrix and use it to purify your 

antibodies. Standard coupling chemistry 

Removal of E. coli- 

Contaminant Antibodies 

• To remove contaminant 

antibodies from a protein 

Affinity Purification of Ab 

• bioWorld can perform affinity 

purification to make your 


expressed and produced in E. coli and used as an antigen for antibody 

production. Standard coupling chemistry 

sample free of other contaminating antibodies deliver peptide-specific 

antibodies. 



FlexArm Linked Affinity Matrix 

• bioWorld can link your peptide 


(may be submitted or produced 

by bioWorld) to a bioAffinity matrix using flexArm linker to provide 

hydrophilic spacer and acheive highly efficient matrix binding. See 

FlexArm peptide synthesis for more information, page 134. 

Ubiquitin-Binding Protein Pufification 

• We can purify ubiquitinylated 


protein/enzymes from your 

sample using ubiquitin affinity chromatography. Useful for ubiquitin 

activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin 

ligases (E3’s) and ubiquitin terminal hydrolyases (ucms). 

Calmodulin-Binding Protein Purification 

• bioWorld purify calmodulin 


can binding proteins using 

calmodulin affinity chromotography. These proteins can be further 

analysed and identified using MASS spec. 

Cleaning of Specific Antibodies 

• bioWorld labs provide affinity purification services by using a unique 

peptide-agarose coupling using a flexible arm PEG flexArm linker to 

allow better binding of antigen to yield highly specific 

polycolonal antibodies. 

Per 1ml serum 

#500542 KLH $ 285.00 

To deplete KLH Abs 

Per 1ml serum 

#500543 E. Coli $ 285.00 

To deplete E. Coli Abs 

depletion 



Matrix Ligand bioAffinity Matrix 

Protein 

Impurities 

Elution Buffer 

131 

Pure Protein

Custom Peptide Synthesis customlabservices 



bioWORLD’s Custom Peptide Synthesis service is perfect for any research facility 

looking for the highest quality, low cost service available. For unique & larger 

peptides, as well as specific peptide modifications, please contact us. 

You can use the Peptide Synthesis and Antibody Production Quote forms. 

www.bio-world.com or www.peptidxpress.com or call 1.888.bio.PLUS. 

Antigenic Epitope Analysis (*free) 

• Antigenic analysis of a protein 

sequence to determine the 

most antigenically unique regions of the protein. 

#501138 service $ 100.00 

• Results can be used for further peptide synthesis & antibody production. 

* If peptide synthesis or antibody production is contracted through 

bioWORLD or PeptideExpress, the analysis fee is waived. 

Computational Analysis 

• The latest software is used to 

analyze your DNA or protein 

sequence to project the physical characteristics of the expressed protein 

including extinction coefficient, isoelectric point, charge, 

hydrophobicity, & secondary structure. 

• BLAST is performed to compare your sequence to published sequences 

to determine common & species-specific regions to aid with the design 

of peptides. 

• Identify various protein motifs to aid in the identification of your 

protein function. 

• A complete restriction site analysis is performed to aid in vector 

construction. 

#501140 service $ 495.00 

• A search for silent mutations that result in the appearance or 

disappearance of restriction sites without changing the protein sequence. 

• Oligonucleotides for PCR amplification, sequencing primers, & 

hybridization probes are designed & analyzed for melting temperatures. 

Peptide Synthesis and Purification 

• The peptides are produced using solid phase peptide synthesis. Fmoc 

is utilized as the amino protecting group & TFA is employed to cleave 

the peptide. The peptide is delivered unpurified (˜65% purity) and 

desalted but can be furthur purified to suit your research applications. 

Purity of all bioWORLD’s peptides are tested using HPLC & MASS 

Spectrometry. 

• The price per residue is for peptide sequences 8-20 amino acids in 

length. Peptides having less than 8 or more than 20 residues will have a 

slightly higher cost. 

• Discrete PEG (polyethylene Glycol) residues can be incorporated in a 

peptide to increase its solubility both to increase its bioactivity and 

further linkage with any carrier protein to enhance antigenicity. 

(see page 134) 

Synthesis of 8-30 residues #501109 per residue $ 15.00 

• (desalted) 

70% Purity #501101 process $ 150.00 

• 5-25mg peptide yield. 





For quotes & order forms, please visit: 

www.bio-world.com • www.peptideexpress.com 

# 

132 

> 

accelerate: molecularlifescienceresearch

Custom Peptide Synthesis (contd.) 

Peptide Modifications 

Acetylation 

Amidation (C-termiinal) 

Anilide (C-terminal) 

Benzyloxcarbonylation 

Biotin 

Dansylation 

#501111 $ 25.00 

#501112 $ 25.00 

#501110 $500.00 

#501124 $100.00 

#501113 $ 75.00 

#501114 $100.00 

MAP (multiple antigenic peptide) System 

• A revolutionary antigen system for high titer specific antibody 

production without conjugation to carrier proteins. 

• Allows for production of highly efficient immunogens. 

• Peptides can be synthesized on a symmetric or asymmetric lysine core 

with amino terminals (branches) available for peptide attachment. 

• Responses to MAP antigens are usually quick & potent, yielding higher 

titer than those produced by hapten-carrier conjugates. 

• Peptides >15 amino acids yield better antibody production. 

• MAP system set up fee is required for all MAP peptides. 


Dinitrobenzolylation (DNP) 

Fatty Acid 

Formylation 

Nitronation 

Phosphorylation - Serine 

Phosphorylation - Threonine 

Phosphorylation - Tyrosine 

Succinylation 

Sulfonation 

Fluorescene/Dye Labeling 

#501115 $ 60.00 

#501125 $ 75.00 

#501116 $ 50.00 

#501126 $100.00 

#501117 $150.00 

#501118 $150.00 

#501119 $150.00 

#501122 $ 50.00 

#501123 $500.00 

Set Up Fee #501090 fee $ 75.00 

MAP Synthesis #501100 per residue $ 22.00 

Asymmetric #501072 4 branches $ 265.00 

#501078 8 branches $ 295.00 

Symmetric #501075 4 branches $ 265.00 

#501080 8 branches $ 295.00 

For hydrophylic MAPs see page 134 


Aminocoumarin 

#501131 $200.00 

Fluorescein 

#505150 $150.00 

Lissamine Rhodamine 

#501134 $ 75.00 

NBD 

#501136 $250.00 

Tetramethylrhoddamine 

#501137 $200.00 

Custom modifications & conjugation 

services available. 

Contact: tech@bio-world.com 

(Note: MAPS can create artifical antigenic epitopes) 

see flexiMAP, page 134 

133

Custom Peptide Synthesis customlabservices 

FlexiPep Service 

• Peptides are synthesized on the 

ends of flexible dPEG spacer 


arms. The spacer arms remain attached to your peptide after synnthesis, 

increasing the solubility of the peptide. This also provides a point of 

attachment for conjugation to other ligands. FlexiPep peptides are 

more antigenic. 

FlexiMAP Service 

NH-Fmoc - Peptide 

• Peptides are synthesized on the 


ends of flexible dPEG spacer 

arms. When these complexes are cleaved from their stationary phase, 

they produce a highly soluble multi-antigenic peptide. FlexiMap offers 

enhanced antigenic displays without the need for conjugation to a 

carrier protein. This eliminates unwanted epitopes, creating a more 

precise immune response and produce specific antibodies. 

NH-Fmoc ~ Peptide 

HO 

d(PEG) 4 







Peptide Synthesis and Antibody Production 

• The project includes: Peptide 

#501105 1 rabbit $ 795.00 

Synthesis from 8-20 amino 

acids, KLH conjugation of the #501106 2 rabbits $ 1099.00 

peptide, Ab Production. Protocol is approximately 90 days-including 

Peptide Synthesis & Ab Production, a positive control bleed, three test 

bleeds & boosts, a production bleed & a final exsanguination bleed. 

• The project is delivered in 2-3 shipments, (shipped on dry ice, costs 

are extra) 

• Each Peptide Synthesis & Ab Project is held in strict confidence & 

complete records provided upon completion. 

• The antisera can be further purified and or lypholized for overseas 

shipments. 

Peptide dPEG Conjugation: KLH / BSA 

• bioWorld offers a unique peptide KLH/BSA conjugation service using 

flexible arm linker of discrete size polyethylene glycol with NHS. 

Such conjugations are often more immunogenic and specific because of 

the high solubility of the peptide and flexibility of the antigenic 

epitope with a hydrophylic spacer arm. 

KLH Conjugation 

#501066 NHS dPEG $ 195.00 

NH-Fmoc - Peptide 


BSA Conjugation 

#501069 NHS dPEG $ 195.00 

enhance antigenicity of your peptide epitope 

Protein Electroelution for Antibody Production 

• Send your sample and we can 

#500615 elution $ 85.00 

run a SDS gel and electroelute 

your protein band for antibody 

#500622 1D gel/elution $ 200.00 

production. 

> 

134 


 

 

 

 

©


Polyclonal Antibody Production Service 

bioWorld produces high quality antibodies at costs substantially lower than 

most laboratory facilities. 

For a Custom Lab Service Quote, access and fill out the Antibody Quote Form at 

www.bio-world.com or call 1.888.bio.PLUS. The quotation will include the following: 

all costs, schedule and delivery of this final product. 

Primary 

antibody 

E 

Substrate 

Secondary 

antibody 

Primary 

antibody 

E 

Substrate 

Biotinylated 

primary 

antibody 

Substrate 

E 

E 

E 

E 

E 

Substrate 

E 

Substrate 

Substrate 


Ag 

Ag 

Ag 

Direc Assay 

Indirect Assay 

Srtaptavidin-Biotin Complex 

• bioWorld offers many hosts suitable for polyclonal antibody production 

including rabbits/ guinea pigs/ mice/ chickens/ goats/ sheep,/and horse. 

• Typically, 1mg of antigen is required for 1 rabbit. Smaller can be 

administered but the immune response of the host may be less potent. 

• Antigens can be provided to bioWorld in a detergent-free buffer 

solution, as a lyophilizate, or a crushed polyacrylamide gel. 

• Each Polycolonal Antibody project is held in strict confidence. 

• bioWorld will not accept antigens that are pathogenic, toxic and/or 

carcinogenic. 

Rabbit #500500 process $ 298.00 

Host Procurement & 

conditioning 

#500518 process $ 45.00 

Pre-inoculation Bleed #500513 process $ 36.00 

Antibody Schedule 

Day 0 

Day 21 

Day 35 

Day 49 

Day 63-85 

Day >90 

Pre-inoculation and initial Inoculation 

First test bleed and boost (using 

complete Freund’s Adjavent) 

Second test bleed and boost 

Third test bleed and boost 

Production Bleed (Included in porject 

cost) and Final Exsanguination Bleed 

(available at additional cost) 

Extended protocols (Extended 

protocols will have additonal fees) 

Antibody Production Service 

Host Disposal #500519 process $ 45.00 

Shipping (dry ice) $ 45.00 / shipment 

• bioWorld offers many additional services to enhance your 

Polycolonal Antibody Project including: ELISA analysis of the test bleeds, 

antibody purification using Protein A or ammonium sulfate 

fractionation lyophilization of antiserum and prolonged host 

maintanence. 

135

Antibody Production Service customlabservices 


Monoclonal Antibody Production Service 

bioWORLD’s custom monocolonal antibodies production service against your 

antigen, includes development of primary antibodies, fusion and ascites 

production. Other services required for screening of hybrids and selection of 

clones can be requested by the researcher as needed. Each Monocolonal Antibody 

project is held in strict confidence and complete record is provided at the 

conclusion of the project. 

Development of Primary Antibodies 

#500520 $85.00/mouse 

ELISA screening to select host for Hybridoma #500525 $150.00/mouse 

Fusion and development of hybridoma #5000530 $ 2,998.00 

ELISA screening for selection of clones 

Cloning of selected hybrids #500534 $ 687.00 

ELISA screening of clones 

Expansion of selected clones 

#500532 $ 725.00 

#500536 $ 350.00 

#500538 $ 498.00 

Monoclonal Antibody Production Project 

Phase 1 #505184 service $ 1,900.00 

Step 1: Immunization 

• Immunization of 5 Balb/c mice. In general we begin with a priming 

dose of 50 to 200 µg followed by several boosting doses each containing 

50 to 200 µg. This regime will continue until a sufficient antiserum titer 

is achieved. 

Step 2: Screening 

• Test bleeds are evaluated by using an indirect ELISA. Optimal ELISA 

conditions are developed to ensure the proper evaluation in other 

assays. 

Step 3: Evaluation 

• Data is evaluated to determine the performance of antiserum. 

Antiserum titers and specific assay performance must be acceptable to 

continue project. 

Phase 2 

#505185 service $ 3,200.00 

Step 4: Hybridoma Fushions 

• A splenocyte fusion is performed on host animals with the best response 

as determined by ELISA or other assay. The lymphocytes are then 

fused to an Sp2/0-Ag14 cell line at a 5:1 ratio using the PEG method. 

Step 5: Expansion & Screening 

• The fusion is plated into 24 or 96 well plates. Plates are monitored for 

growth and fed weekly. Wells with cell growth are screened by ELISA and/or 

other methods at 10-20 days after fusion. 

Ascites production 100ml 

#500541 $ 7.60/ml 

> 

accelerate: monoclonalAntibodyproduction 

Step 6: Subcloning 

• Cells from up to 5 strongly positive parental clones from fusion wells 

are expanded, isotyped and subcloned by limiting dilution. More subclones 

can be processed at your request for additional charge. 

Step 7: Expansion & Screening 

• Subclones are screened by ELISA, positives expanded, and screened 

again. Up to (2) positive subclones from each parental subclone will be 

expanded, frozen, and stored for up to six (6) months.


1-D & 2-D Gel Protein Analysis 

1-D Gel Service 


• 1-D SDS gel running service. 

• Acrylamide gel % indicated at 

the customer’s request 

• Option of 10, 12, wells per comb 

• Large size: 16 x 13cm, small 8 x 8cm 

Silver Stained 

• We provide fully documented 

results with a publication 

quality print & stained gel 

(wet or dry). 

• Special handling of the gel is required to prevent degradation of 

proteins in order to get reproducible results (call for details). 

• Please call for instructions if shipment is radioactive (there is an ` 

additional disposal fee). 

• Gel dimensions: 16 x 13cm 

• A pH gradient blot is provided along with a description of method & 

gel loading information (custom pH gradients are available). 

• Standard IEF tube gel contains 2% (pH 4-8 BDH) ampholines. 

• Molecular weight & internal standard marker are included in each gel. 

• 10 times more sensitive than Coommassie Blue staining (depending on 

the protein). 

• Gives qualitative rather than quantitative results. 

• NOTE: Silver staining is not compatible with mass spectrometry. A 

special silver stain can be substituted if mass spectrometry analysis 

will be performed. Please indicate when ordering. 

Find your spot 

#500620 large $ 120.00 

#500621 small $ 100.00 

#500601 2D gel $ 198.00 

2-D Gel Service 

• Multi-step process used to resolve complex sets of proteins from total 

soluble or membrane protein fractions. 

• Ideal for detecting enzyme isomers, protein phosphorylation, protein 

glycosylation, amino acid microsequencing, enzyme/protein induction 

studies, mutations & other protein modifications as well as a complete 

proteomics profile. 

• bioWORLD provides fully documented results with a publication quality 

print & stained gel (wet or dry). A sample preparation kit (cat# 500627) 

is also available to ensure your protein samples are best suited for 2-D 

Gel Electrophoresis. 

• Avoid degradation of proteins in order to get reproducible results. 

• Gel dimensions: 16 x 13cm. 

• A pH gradient blot is provided along with a description of method & 

gel loading information (custom pH gradients are available). 

• Standard IEF tube gel contains 2% (pH 4-8 BDH) ampholines. 

• Molecular weights & internal standard marker are included in each gel. 

Coomassie Blue Staining PVDF 

• Reveals the major proteins in 

#500630 process $ 165.00 

the 2-D patterns & is necessary 

to visualize proteins for N-terminal sequencing. 

• Useful when matching the blot to a duplicate gel & identifying proteins 

for Mass Spectroscopy analysis. The stain disappears during 

western blotting so a color electronic photo should be obtained 

before analysis. 

Coomassie Blue Stained #500600 1 sample $ 195.00 

• (See above description). 

#500602 2-5 sample $ 185.00 

#500604 6+ sample $ 175.00 

Photography/Digital Image #500626 process $ 35.00 

• Excellent color images in .tif or 

.jpeg format. 

• Price includes an image file on a CD & prints, or the file can be 

e-mailed directly to you as requires. 


1-D & 2-D Gel Analysis 

> 


137



Sample Preparation Kit #500627 Kit $ 196.00 

• Provides necessary buffers (SDS, 

Urea, Lysis, Protease Inhibitors, & Nucleases) & components to 

adequately prepare samples for custom electrophoresis services. 

• Adequate material to prepare 10 samples. 

• Buffers must be shipped on dry ice. 

Sample Preparation Service #500611 service $ 145.00 

Silver Stained 2D gel #500601 1 sample $ 198.00 

• (See Silver Stained under 1-D). 

#500603 2-5 sample $ 188.00 

#500605 6+ sample $ 178.00 

Unique Spot Analysis #501139 process $ 298.00 

• bioWORLD provides 

detailed analysis for identification of unique spots in 2-D gels to 

identify a new gene product by comparing two samples. 

• *The gels to be analyzed must be run by bioWORLD.* 

Custom IEF or NEPHGE 

• Isoelectrofocusing or 

#500610 process $ 195.00 

non-equilibrium pH gradient electro-phoresis 

• Used to resolve complex sets of 

proteins from total soluble or membrane protein fractions. 

• bioWorld provides fully documented results with publication quantity 

print and stained gel (wet or dry). 

• Molecular weight and internal standard markers are included. 

• bioWorld can prepare the sample for you. for a fee of $140.00. 

Transblotting onto Polyvinyl difluoride (PVDF) 

• PVDF is directly compatible with 

#500629 process $ 75.00 

protein gel sequencers & binds 

proteins more tightly than nitrocellulose giving greater recoveries. 

• Carried out overnight according to standard procedures using a tris/ 

glycine/methanol buffer system without SDS. 

• The dried unstained blot is returned to the client unless BP-2 

or processed for Mass Spec analysis. 

• Custom blotting services are available upon request, please inquire to 

change the procedure to your specifications. 

MALDI-TOF Mass Spectrometry Analysis 

• Analysis of a peptide in solution 

#501129 1 sample $ 295.00 

or eluted from gel (1-D or 2-D). 

• Raw data is provided for #501130 2+ samples $ 195.00 

further analysis & interpretation. 

• Data analysis available for an additional cost (please call for pricing) 

LC-MS/MS Protein Identification Service 

• Protein identification service 

#501127 1 sample $ 495.00 

includes sample preparation, 

digestion, peptide extraction #501128 2+ samples $ 375.00 

from 1D or 2D gel, nano- LC-MS/MS analysis and database 

interrogation. 

Duplicate Gel Service 

• 1 duplicate of any gel 

• Price per duplicate. 

#500606 1 gel $ 145.00 

#500613 2-4 gels $ 135.00 

#500609 5+ gels $ 120.00 

138



Vector Construction 

• Provide the gene insert either 

as purified DNA, within a 

purified plasmid or contained in a slab culture and bioWorld will excise 

the insert and ligate it into an appropriate bacterial expression vector. 

• The experimental design of the ligation may be facilitated using the 

latest software to design new recombinant DNA molecules or recreate 

gene segments using oligos with appropriate restriction sites and 

features that promote or enhance expression. 

• Transformation of E. coli using the subcloned plasmid will be performed. 

• Colonies will be screened using conventional methodologies. 

• Slab cultures require plasmid isolation prior to subcloning. 

Site Directed Mutagenesis 

• Single mutation per plasmid 

• Gene mutation, codon 

modification, or restriction site modification on any vector that is 

provided as plasmid DNA. 

#505021 service $ 600.00 

#505011 service $ 800.00 

Genomic DNA Library Construction 

• A genomic library is constructed 

#505020 service $ 3,600.00 

in lambda phage or EMBL vector. 

• The DNA supplied is partially or fully digested with either Eco RI or Sau 

3A-I, size fractionated and ligated into lambda phage vectors of choice, 

packaged and titrated. 

• A library of 10 6 independent recombinants is provided. 

• This library is amplified by infecting respective host strains of E. coli. 

DNA Sequencing 

• DNA sequencing is conducted 

#505026 per reaction $ 6.50 

by our highly trained and 

experienced staff with the Sanger’s dideoxy sequencing protocol using 

ABI’s automatic sequencer. 

• bioWorld will synthesize internal primers or subclone the fragment, if 

needed, or will purify the plasmid DNA for sequencing purposes. 

• Both strands of DNA are sequenced depending upon the need of the 

investigator. 

• Price includes one reaction for one sequence which equals,/a minimum 

of 500 base pairs. 

• Minimum charge $100/projects 

cDNA Library Construction 



Baculovirus Cloning Expression 

• A DNA insert from the vector 

#505024 service $ 950.00 

provided is isolated and 

subcloned into a Baculovirus expression vector. 

• The recombinant plasmid is transfected into SF9 or SF21 insect cells 

along with linearized wild type insect virus. 

• The gene product of interest is identified by western blot analysis (if 

the antibody is provided) for the expression of the recombinant protein. 

• A high titer virus stock in 50ml medium is provided to researcher for 

future studies. 

• bioWorld is able to create a 


cDNA Library of your choice, 

starting from 15µg of mRNA. 

• The library is produced in Eco RI or Xho I site of the vector. 

• The cDNA Library is made after 2 rounds of normalization (COT 100) 

thus enabling equal representation of low and high abundance cDNA 

clones. 

• The library provided contains 10 6 primary recombinants in the selected 

vector. bioWorld requires at least 1mg total RNA or 10µg of poly (A+) 

RNA. 

• The library will be ready for transfection purposes 

139


mRNA Isolation 

• High quality mRNA for 

#505014 1 Sample

Index 

Index 

1,5-Naphthalenedisulfonate Salt................... 116 

1,10 Phenanthroline...................................... 32 

1-D Gel Electrophoresis................................ 119 

1H-Tetrazole.................................................. 107 

2-D Gel - Silver Stained................................. 138 

2-D Gel Electrophoresis................................ 137 

2’-Deoxyadenosine Monohydrate.................. 111 

2’-Deoxycytidine Hydrochloride..................... 111 

2’-Dioxy-D-Ribose.......................................... 111 

2’-Deoxyguanosine Monohydrate.................. 111 

2’-Deoxythymidine.......................................... 111 

2’-Deoxyuridine.............................................. 112 

2-Mercaptoethanol......................................... 115 

3,3’,5,5’-Tetramethyl Benzidine...................... 119 

4-Chloro-1-Napthol........................................ 111 

4-Methylumbelliferyl Phosphate..................... 115 

5-Fluorouracil................................................. 113 

5-Sulfosalicylic Acid....................................... 119 

6-Mercaptopurine,.......................................... 115 

ABTS............................................................. 89 

ACES............................................................. 108 

Acetate Buffer ............................................... 81, 72 

Acetylsalicylic Acid........................................ 108 

α-Chymotrypsin............................................. 88 

Acidity Test Kit............................................... 53 

Acriflavine Neutral (Euflavin).......................... 108 

Acrylamide..................................................... 108 

Acrylamide Starter Kit................................... 46 

Acrylamide/BIS ............................................. 108 

Actinomycin D................................................ 96 

Activated Charcoal Acid Washed................... 110 

Adenine (6-aminopurine)............................... 108 

Adenosine...................................................... 108 

ADP, Adenosine-5’-diphosphate.................... 96 

AEBSF........................................................... 108 

Affinity Chrometography................................. 4-22 

Agar............................................................... 126 

Agarose 3:1 High Resolution ........................ 108 

Agarose Gel Loading Dye ............................. 68 

Agarose I....................................................... 109 

Agarose II...................................................... 109 

α-Ketoglutaric Acid........................................ 114 

Albumin- Bovine Fraction V............................ 109 

Alkaline DNA Gel Running Buffer.................. 68 

Alkaline Phosphatase.................................... 67 

Alkalinity Test Kit............................................ 53 

Ammonium Acetate........................................ 109 

Ammonium Acetate Solution......................... 109 

Ammonium Carbonate .................................. 109 

Ammonium Chloride...................................... 109 

Ammonium Hydrogen Carbonate.................. 109 

Ammonium Ntrate.......................................... 109 

Ammonium Persulfate ................................... 109 

Ammonium Phosphate................................... 109 

Ammonium Sulfate......................................... 109 

Amoxicillin ..................................................... 96 

AMP (Adenosine-5-monophosphate)............. 108 

AMP Buffer Concentrate................................ 72 

AMP Sepharopore....................................... 18 

Amphotericin B .............................................. 96 

Ampicillin........................................................ 96 

AMPSO.......................................................... 109 

Antibody Production....................................... 135-136 

Antibody Purification With Protein A............... 131 

Antibody Purification With Protein G.............. 131 

Anti-Flag-Tag.................................................. 93 

Antigen Affinity Purification............................ 131 

Antigen-Antibody Dissociation Kit.................. 65 

Antigenic Epitope Analysis............................. 132 

Anti-GFP-Tag.................................................. 93 

Anti-MYC-Tag................................................. 93 

Antipain.......................................................... 31 

Anti-Phosphoserine Ab................................... 92 

Anti-Phosphothreonine.Ab............................. 93 

Anti-Phosphotyrosine Ab................................ 93 

AP-Blue.......................................................... 89 

Apoptotic DNA Ladder Kit.............................. 56 

Apramycin Sulfate.......................................... 97 

AP-yellow....................................................... 89 

Arachis hypogaea (Peanut) Lectin................. 26 

Arginine Sepharose.................................... 9 

Arrayhyb Kit ................................................... 57 

ATP (Adenosine-5’-Triphosphate)................... 96 

Auraquent.................................................... 34 

Avidin Sepharopore..................................... 12 

Avidin.............................................................. 109 

Bacitracin........................................................ 97 

Bacteriological Agar........................................ 122 

Baculovirus Cloning/Expression..................... 139 

BASTA............................................................ 129 

BBS/Casein.................................................... 77 

BBS/Fish Gelatin............................................ 77 

BCA Protein Assay......................................... 35 

BCIP................................................................ 89 

BCIP/INT......................................................... 89 

BCIP/NBT........................................................ 89 

Benzamidine Hydrochloride........................... 109 

Benzamidine Sepharose............................. 9 

Benzoic Acid................................................... 109 

BES Buffer...................................................... 73, 110 

β-Galactosidase............................................. 110 

Bestatin........................................................... 31 

Betaine........................................................... 110 

Bicarbonate Buffer.......................................... 78 

bioAlginate Beads(BAB)................................. 5 

bioBlock.......................................................... 51 

bioFectin......................................................... 58 

bioLAM (Lectin Affinity Mtrix)....................... 14 

bioSilica Beads............................................... 6 

Biotin (Vitamin H) .......................................... 98 

bioTRAP......................................................... 51 

biozol Plant RNA Isolation Kit........................ 60 

Bis-Tris........................................................... 110 

Black Pearl Magnetic Beads....................... 6 

Black Pearl-A (Protein A)............................ 19 

Black Pearl CAM (Calmodulin)....................... 19 

Black Pearl-G(Protein G)............................ 19 

Black Pearl-L(WGL).................................... 19 

Black Pearl-S(Streptavidin)......................... 19 

Bleomycin Sulfate........................................... 97 

Blotto In PBS.................................................. 78 

Blue Gal......................................................... 110 

Blue Sepharopore ...................................... 10 

Bluo-Gal ........................................................ 89 

Bone PCR Kit................................................. 73, 77 

Borate Buffer.................................................. 73, 77 

Boric Acid....................................................... 110 

Bovine Albumin Fraction V.............................. 110 

Bovine Albumin Standard............................... 35 

Bovine Serum Albumin................................... 98 

Bradford Protein Assay ................................. 35 

BRIJ-35.......................................................... 105 

BSA-Blocking Buffer.... ...... ........................... 79 

Calcium Carbonate........................................ 110 

Calcium Chloride............................................ 98 

Calcium Citrate Tetrahydrate.......................... 110 

Calcium D-Gluconate..................................... 98 

Calcium Nitrate............................................... 110 

Calcium Phosphate........................................ 110 

Calmodulin Binding Protein Purification......... 131 

Calmodulin Microtiter Plate............................ 24 

Calmodulin Sepharose 4B.......................... 13 

CAPS............................................................. 105 

CAPSO........................................................... 105 

Carbenicillin................................................... 97 

Carbonate Buffer............................................ 73 

Carboxin......................................................... 97 

Carboxylated Microtiter Plates........................ 23 

Carrageenan.................................................. 110 

Casamino Acids............................................. 110 

Casein Blocker................................................ 79 

Caspase Apoptotis Assay Kit......................... 55 

CB Protein Assay........................................... 34 

CDI-Activated Sepharose 4B...................... 7 

cDNA Library Construction............................ 139 

Cefotaxime Sodium........................................ 98 

Cell Buster Kits........................................... 29 

Cellulase (Onozuka R-10).............................. 88 

Cephalexin..................................................... 98 

Cesium Chloride............................................ 110 

Cesium Sulfate............................................... 110 

Cetyldimethylethylammonium Bromide.......... 105 

Cetylpyridinium Chloride................................ 110 

Cetyltrimethylammonium Bromide................. 105 

CHAPS.......................................................... 105 

CHAPS Lysis Buffer...................................... 81 

CHAPSO....................................................... 106 

CHES............................................................ 106 

Chloramphenicol........................................... 98 

Chlorhexidine Digluconate............................ 98, 111 

Chloride Analysis Test Kit............................. 53 

Chlortetracycline .......................................... 98 

Cholesterol.................................................... 111 

Choline Chloride........................................... 111 

Citrate Buffer ................................................ 73 

Citric Acid...................................................... 111 

Cleanascite................................................... 33 

Cleaning of Specific Antibodies..................... 131 

Clindamycin ................................................. 17 

CM Sephapore.......................................... 17 

CNBr-activated Sepharose 4B.................. 7 

Cobalt Chloride Hexahydrate........................ 99 

Colchicine..................................................... 98 

Colloidal Gold Kit......................................... 34 

Column-Protein-Concentrate........................ 33 

Computational Analysis................................ 132 

Concanavalin A Lectin.................................. 26 

Concanavalin A Micro Plate.......................... 24 

Concanavalin A Sepharose 4B.................. 13 

Coomassie Brilliant Blue G-250.................... 48 

Crack Free Gel Drying Kit............................. 50 

Creatine Phosphate...................................... 111 

CTP (Cytidine-5’-Triphosphate).................... 111 

CU-Alginate.................................................. 5 

Cupric Sulfate, Pentahydrate........................ 111 

Cyanocobalamin........................................... 111 

Cytoscan Cytotoxicity Assay Kit.................... 94 

Cytosine........................................................ 111 

Cytox Cytotoxicity Assay Kit, LDH................. 94 

D (+) Galactose............................................. 113 

Dansyl Chloride............................................ 111 

d-ATP............................................................ 111 

Datura Stramonium Lectin............................ 26 

D-Biotin, Vitamin H........................................ 110 

d-CTP............................................................ 111 

DEA Buffer ................................................... 81 

DEAE Dextran............................................... 111 

DEAE Sepharopore .................................. 10 

Denatured DNA............................................ 100 

Denaturing Lysis Buffer................................. 81 

Deoxycholic Acid .......................................... 106 

DEPC-Treated Water..................................... 74 

DEPC, Diethylpyrocarbonate....................... 112 

Destomycin................................................... 99 

Detergent Kit................................................. 31 

Detergent-out Kit........................................... 31 

Dextran.......................................................... 112 

Dextropore Columns.................................. 33

D-Fructose.................................................... 113 

D-Glucose..................................................... 113 

d-GTP............................................................ 112 

Diaminobenzidine Substrate System............ 94 

DICAMBA...................................................... 112 

Diethanolamine............................................. 112 

Diethylamine.................................................. 112 

DIPSO............................................................ 106,112 

DissolveAway Kit........................................ 29 

Dissolved Oxygen Test Kit............................. 53 

DL- Malic Acid............................................... 115 

D-Luciferin (Free Acid).................................. 90 

D-Mannitol..................................................... 115 

D-Mannose.................................................... 115 

DMEM........................................................... 122 

DMF (N’,N- Dimethylformamide)................... 112 

DMSO (Dimethylsulfoxide), .......................... 112 

DNA Denatured Calf Thymus........................ 112 

DNA Denatured Salmon Sperm.................... 112 

DNA Denaturating Solution........................... 67 

DNA Fish Testes............................................ 112 

DNA Pure Yeast Genomic Kit........................ 56 

DNA Sequencing........................................... 139 

DNA Superladder High ................................. 61 

DNAse .......................................................... 122 

Dolichos Biflorus Lectin................................. 26 

Dotmetric Protein Assay ........................... 36 

Doxorubicin HCL........................................... 99 

Doxycycline HCL........................................... 99 

D-Pantothenic Acid........................................ 116 

D-Ribose........................................................ 118 

D-Sorbitol...................................................... 118 

DTBP............................................................. 42 

DTE (DL-Dithioerythritol)............................... 112 

DTSSP........................................................... 42 

DTT, DL-Dithiothreitol.................................... 112 

DTTP.............................................................. 112 

Duplicate Gel Service.................................... 138 

E-64............................................................... 31 

E.coli Lysis Buffer.......................................... 81 

E.coli Protein Sepharopore........................ 3 

EAH Activated Sepharose ....................... 7 

ECH Activated Sepharose......................... 8 

EDTA ............................................................ 112 

EGTA............................................................. 74, 112 

EIA Buffer-Electroporesis unit 46.................. 82 

EMCH............................................................ 41 

Enhanced Silver............................................. 49 

Enzyme Stabilizer Empty columns................ 34 

Epitope Tag Antibodies Anti-His6-Tag........... 93 

Epitope Tag Antibodies Anti-S-Tag................ 93 

Epoxy-Activated Sepharose 4B................. 8 

Erythromycin.................................................. 99 

Ethylene Glycol.............................................. 112 

Express Coomassie Stain............................. 48 

E-Z Protein Stabilizer................................. 34 

Fast Silver...................................................... 49 

Fasttrack Transformation Kit.......................... 57 

Femtolucent Detection Kit.............................. 50 

Ferric Ammonium Sulfate ............................. 112 

Ferric Chloride............................................... 112-113 

Ferric Nitrate.................................................. 112-113 

Ferric Sulfate.................................................. 112-113 

Ferrous Sulfate............................................... 113 

Fetal Calf Bovine Serum ............................... 124 

Ficoll 400....................................................... 113 

Firepol (Thermostable Taq polymerase)........ 64, 88 

Fire Tag-AP HRP............................................ 39 

Fish Gelatin................................................... 101 

Five Minute Reversible Stain......................... 48 

flexArm Linker Affinity Matrix..................... 131 

flexArmLinkers........................................... 42-44 

FlexiMAP................................................... 134 

FlexiPep..................................................... 134 

Fluridone....................................................... 113 

Foam Free................................................. 31 

FOCUS...................................................... 30 

FOCUS-Phosphorich................................. 32 

FoldRight Kit.............................................. 36 

Folic Acid....................................................... 113 

Formaldehyde (4%) In PBS........................... 30 

Formaldehyde Determination Test Kit............ 53 

Formamide..................................................... 113 

Facose Binding Microplate............................. 24 

Free Chlorine Determination Test Kit............. 54 

Fungi Gone.................................................... 129 

G-418 Disulfate............................................. 99 

Galactosidase................................................ 88 

GDP (Guanosine-5’-Diphosphate)................ 102 

Genomic DNA Extraction Kits........................ 57 

Gebaflex Kits.............................................. 45 

Gelatin........................................................... 113 

Gelatin Sepharopore.................................. 10 

Gelatine- Blocking Buffer .............................. 79 

Gel Drying..................................................... 47 

Gene Bullet................................................ 57 

Gene Capsule .............................................. 58 

GeneExit DNA Isolation Kit........................ 57 

Genomic Library Construction....................... 139 

Gentamicin Sulfate........................................ 99 

Glass Milk...................................................... 63 

Glucose Oxidase........................................... 88 

Glucose/Tris/EDTA ....................................... 67 

Glutathione.................................................... 113 

Glutathione Sepharose 4B......................... 11 

Glycerol......................................................... 113 

Glycine.......................................................... 113 

Glycine Buffer................................................ 74 

Glycine max (Soybean) Lectin...................... 26 

Glycoprotein Isolation Kit............................... 32 

Glycoprotein Staining Kit............................... 50 

Glycyl-Glycine............................................... 113 

GMP (Guanosine-5’-Monophosphate).......... 102 

Grace’s Media .............................................. 124 

Grittonia Lectin.............................................. 27 

GTP (Guanosine-5’-triphosphate)................. 102 

Guanidine ...................................................... 113-114 

Guanine......................................................... 114 

Guanosine..................................................... 114 

Ham’s Nutrient Mix........................................ 124 

Heparin Microtiter Plate................................. 24 

Heparin Sepharose.................................... 11 

HEPES...................................... .................... 114 

HEPES, Sodium Salt..................................... 114 

HEPPS ......................................................... 114 

HEPPSO........................................................ 114 

Heterobifunctional Crosslinkers..................... 41 

High Binding Microtiter Plate......................... 23 

Histalon Affinity Purification Kit.................. 38 

Homobifunctional Crosslinkers...................... 42 

Homogerizer, Omni........................................ 29 

Horse Blocker................................................ 80 

Hot Firepol (Taq polymerase)..................... 88,64 

Hotstart Firepol.......................................... 64, 88 

Human Follicle Stimulating Hormone............ 91 

Human Goserelin.......................................... 91 

Human Leuprolide......................................... 91 

Human Leutenizing Hormone Releasing 

Hormone...................................................... 91 

Human Octreotide.......................................... 91 

Human Oxytocin............................................ 91 

Human Protirelin............................................ 91 

Human Serum .............................................. 125 

Hybridization Cocktail.................................... 58 

Hygromycin B Hydrochloride......................... 100 

IBS/Casein.................................................... 78 

IBS/Fish Gelatin............................................ 78 

ICL................................................................ 40 

IEF................................................................. 138 

IEF Anode Buffer .......................................... 69 

IEF Cathode Buffer ....................................... 69 

IEF Sample Buffer ........................................ 69 

IgG Sepharopore 4B.................................. 14 

Imidazole....................................................... 114 

Immunomax Kit.......................................... 65 

In Gel Protein Digestion................................. 37 

Indoxyl-Gal. ................................................... 89 

Indoxyl-Glucoside ......................................... 89 

Iodine Test Kit................................................ 54 

IPG-Strip Equilibration Buffer........................ 69 

IPL-41 Insect Medium................................... 125 

IPTG.............................................................. 90 

Iron Chemical Test Kit.................................... 54 

Isopropyl Alcohol........................................... 114 

Jacalin Coated Microtiter Plate...................... 24 

Kanamycin Sulfate........................................ 100 

Kinetin (6-Furfuryl-aminopurine)................... 114 

KLH Sepharose-4B.................................... 14 

L-(+)-Lactic Acid............................................ 114 

Laemmli Buffer ............................................. 69 

L-Alanine....................................................... 109 

Lanthanum Acetate....................................... 114 

L-Arginine...................................................... 109 

L-Ascorbic Acid, (Vitamin C).......................... 109 

L-Asparagine................................................. 109 

L-Aspartic Acid.............................................. 109 

LB Broth......................................................... 126 

L-Cysteine ..................................................... 111 

LDS, Lithium Dodecyl Sulfate........................ 106 

Leaf PCR Kit.................................................. 64 

Leibovitz’s Media.......................................... 125 

Lens culinaris Lectiln ................................... 27 

Leupeptin ...................................................... 114 

L-Glutamic Acid............................................. 113 

L-Glutamine................................................... 113 

L-Histidine...................................................... 114 

Lincomycin .................................................... 100 

Linoleic Acid.................................................. 114 

L-Isoleucine................................................... 114 

Lithium Chloride............................................. 114 

L-Leucine....................................................... 114 

L-Lysine......................................................... 115 

L-Methionine.................................................. 115 

L-Phenylalanine............................................. 116 

L-Proline........................................................ 117 

L-Serine......................................................... 118 

L-Threonine................................................... 119 

L-Tryptophan................................................. 120 

L-Tyrosine...................................................... 120 

Luminol.......................................................... 115 

L-Valine.......................................................... 120 

Lysozyme...................................................... 88 

Macerozyme R-10......................................... 88 

Magenta-Caprylate........................................ 90 

Magnesium Acetate....................................... 115 

Magnesium Chloride...................................... 115 

Magnesium Sulfate........................................ 115 

MagneTalon Beads.................................... 20-22 

Malachite Green Oxalate............................... 115 

Malachite Green Phosphate Assay Kit.......... 94 

Malate Dehydrogenase................................. 88 

MALDI-TOF Mass Spec Analysis.................. 138 

Maleic Acid Hydrazide................................... 115 

Maleimide Microtiter Plate............................. 23 

Manganese Sulfate..................................,..... 115 

Mannose Sepharopore..........................,.... 17 

MAP Synthesis.............................................. 133 

MASS Silver.................................................. 50 

MBS............................................................... 41 

McCoy’s Media.............................................. 125

MCDB 131 Medium....................................... 126 

Medium 199................................................... 126 

Mercaptoethanol........................................... 115 

MES............................................................... 115 

Meta DNA Purificaton Kit............................... 56 

Metal Chelating Sepharopore.................... 11 

Methotrexate.................................................. 100 

Metribuzin...................................................... 115 

Metronidazole................................................ 100 

Miconazole Nitrate......................................... 100 

Mitomycin C................................................... 101 

Molybdic Acid................................................ 115 

Monoclonal Ab Production Service............... 136 

Monocyte Chemotactic Protein-1 (CCL2)..... 91 

MOPS............................................................ 74, 115 

MOPSO......................................................... 115 

mRNA Isolation.............................................. 140 

MS Medium.................................................... 128-129 

MUG.............................................................. 90 

MYO-Inositol.................................................. 116 

NAD............................................................... 116 

NAD Sepharopore..................................... 18 

NADH............................................................ 116 

NADP............................................................. 116 

NADP Sepharopore................................... 18 

Nalidixic Acid................................................. 101 

NAP Blocker.................................................. 40 

Naphthol ....................................................... 116 

Naphthylacetamide........................................ 116 

NBT............................................................... 90 

N-Dodecylβ-D-Maltoaside............................. 106 

NEPHGE....................................................... 138 

Neomycin Sulfate.......................................... 101 

Neutral Dissociator........................................ 38 

NHS-Activated Sepharose 4B................... 8 

Nicotinamide................................................. 116 

Nicotinic Acid................................................ 116 

Ninhydrin....................................................... 116 

Nitrate Detection Kit...................................... 54 

Nitrocellulose Stripping Buffer....................... 80 

Nitrofurantoin................................................ 116 

N-Octyl-β-D-Glucopyranoside....................... 107 

Non Interfering Protein Assay........................ 35 

NonIdet P-40................................................. 106 

Northern Blot Analysis.................................. 140 

NP-40 Lysis Buffer ....................................... 83 

Nucleic Dotmetric Basic Kit........................... 59 

Nylon Membrane Stripping Buffer ................ 58 

Nystatin......................................................... 101 

Omni PAGE Mini/maxi Electrophoresis......... 46 

Omni Tip Kit............................................... 29 

O-Nitrophenyl-β-D-Galactopyranoside.......... 90 

O-Phenylenediamine (OPD)......................... 90, 116 

Orange-G Loading Dye................................. 69 

Orange-G Blue Loading Dye......................... 69 

Orgosol-Protein Concentrator....................... 31 

Osmocons Kit............................................ 36 

Ovalbumin..................................................... 34 

Oxalic Acid..................................................... 116 

Paclobutrazol................................................. 116 

p-Aminobenzoic Acid. (PABA)........................ 109 

Paraformaldehyde (4%) In PBS .................... 75 

Paraplast........................................................ 116 

Paromomycin Sulfate..................................... 101 

PBS ............................................................... 69 

PBS/Casein.................................................... 73 

PBS/Fish Gelatin............................................ 73 

PBS-T ............................................................ 75 

Pectinase........................................................ 88 

Penicillin G ..................................................... 101-102 

Penicillin V....................................................... 102 

Pepsin 1:3000................................................. 88 

Pepstatin A..................................................... 31 

Peptide Conjugation To BSA/KLH.................. 134 

Peptide dPEG Conjugation............................ 134 

Peptide Pre-cleaning Buffer........................... 78 

Peptide Synthesis & Ab Production............... 134 

Peptide Synthesis, Purification ...................... 132 

Peptone Water................................................ 116 

Perfect Focus Protein Kits.......................... 30 

Peroxidase Removal Kit.................................. 49 

Peroxidase, Horseradish................................. 88 

Phase-Guard............................................... 54 

Phenazine Methosulfate................................. 116 

Phenol Red..................................................... 116 

Phenol, Crystalline.......................................... 116 

Phenylacetic Acid ........................................... 116 

Phleomycin..................................................... 102 

Phloroglucinol................................................. 116 

Phosphate Buffer ........................................... 69 

Phosphate Test Kit.......................................... 48 

Phosphotot.................................................. 50 

Pick Pen...................................................... 22 

Picloram.......................................................... 116 

Pink DNA Cleanup Kit.................................... 58 

PIPES.............................................................. 117 

Plant Genomic DNA Extraction...................... 50 

Plasmid DNA Isolation Solution...................... 62 

Plasmid Purification Powerprep...................... 55 

PMSF.............................................................. 32 

p-nitrophenyl Phosphate................................. 90,94 

Poly Histalon................................................... 93 

Polyethylene Glycol ....................................... 117 

Poly-L-Arginine Coated Micro Plate............... 24 

Poly-L-Lysine Coated Micro Plate.................. 24 

Polymyxin B Sulfate....................................... 102 

Polystyrene Beads......................................... 6 

Polyvinylpyrrolidone (PVP)............................ 117 

Ponceau S...................................................... 48 

POPSO........................................................... 117 

Potassium Acetate ......................................... 117 

Potassium Cacodylate.................................... 117 

Potassium Carbonate..................................... 117 

Potassium Chloride ........................................ 117 

Potassium Ferricyanide.................................. 117 

Potassium Hydroxide...................................... 117 

Potassium Iodide............................................ 117 

Potassium Nitrate........................................... 117 

Potassium Permanganate.............................. 117 

Potassium Persulfate...................................... 117 

Potassium Phosphate..................................... 75, 117 

Potassium Sorbate......................................... 117 

Precast Polyacrylamide Mini Gels.................. 46 

Primary Amine Microtiter Plate....................... 23 

Prionex........................................................... 40 

Procipitate................................................... 33 

Proline............................................................ 36 

Propylene Glycol............................................ 117 

Protease Inhibitors......................................... 31-32 

Protein A........................................................ 88 

Protein A Coated Micro Plate......................... 24 

Protein A Sepharose 4B............................. 15 

Protein A-gold................................................. 60 

Protein A-Red Sepharopore........................ 15 

Protein Blots.................................................... 40 

Protein Concentrator Kit................................ 33 

Protein Controls.............................................. 37 

Protein Coupling Kit........................................ 84 

Protein Electroelution...................................... 134 

Protein G Coated Micro Plate......................... 25 

Protein G Sepharose 4B............................. 15 

Protein Kinase AKT1/PKB ............................. 92 

Protein Markers.............................................. 47 

Protein Master Strategy.................................. 30 

Protein Puification Buffer................................. 78 

Protein Standards........................................... 35 

Proteinase K................................................... 88 

Proteomax................................................... 29 

Protoplast Isolation Kit.................................... 50 

Psophocarpus Tetragonolobus Lectin............. 27 

PVDF Coomassie Blue Staining..................... 137 

Pyridoxal-5-phosphate.................................... 117 

Pyridoxine HCI............................................... 117 

Pyruvic Acid.................................................... 117 

QBT Buffer...................................................... 62 

QC Buffer........................................................ 62 

QF Buffer........................................................ 62 

QuantaLeap................................................ 39 

Quantagold................................................... 34 

Quantalite......................................................... 38 

Quick Immuno Detect Kit................................ 37 

Quick Pick Plasmid Kit................................ 22 

Quickblock.................................................. 50 

Raffinose........................................................ 118 

Rapamycin...................................................... 102 

Rapid RNA Purification Kit.............................. 55 

Recombinant Cytomegalo Virus Mosaic......... 91 

Recombinant Dengue Virus NS3.................... 91 

Recombinant Epstein-barr Virus (HHV-4)P23. 91 

Recombinant Hemagglutinin........................... 91 

Recombinant Hepatitis C Virus NS5 ............. 91 

Recombinant Hepatitis B Virus HBE .............. 91 

Recombinant HIV-I GP41................................ 91 

Recombinant HIV-I P24.................................. 91 

Recombinant HIV-II envelope......................... 91 

Recombinant HIV-II GP32............................... 91 

Recombinant HIV-II GP36............................... 91 

Recombinant HTLV-I envelope........................ 91 

Recombinant Human AKT1............................ 92 

Recombinant Human Artemin......................... 91 

Recombinant Human BAFF (BLYS)................ 91 

Recombinant Human Beta Nerve 

Growth Factor................................................ 91 

Recombinant Human CD34............................ 92 



Recombinant Human Chorionic 

Gonadotropin................................................ 92 

Recombinant Human Endocrine Gland 

Vacular Growth Factor.................................. 91 

Recombinant Human Epidermal 

Growth Factor................................................ 91 

Recombinant Human ERK1/MAPK1 ............. 92 

Recombinant Human Glial Neurotropin.......... 91 

Recombinant Human Glucagon..................... 92 

Recombinant Human GRO-ALPHA (CXCL1). 91 

Recombinant Human Growth Hormone......... 91 

Recombinant Human Heat Shock Protein 20. 92 



Recombinant Human HIV-I P55 GAG............ 91 

Recombinant Human Interferon-Alpha 2a...... 91 

Recombinant Human Lymphotactin............... 91 

Recombinant Human Macrophage 

Colony Stimulant.......................................... 91 

Recombinant Human MIG (CXCL9)............... 91 

Recombinant Human Neurotrophin-3............. 91 

Recombinant Human P38 ALPHA/SAPK2..... 92 

Recombinant Human P53.............................. 92 

Recombinant Human Prolactin...................... 91 

Recombinant Human Protein Kinase Alpha... 92 

Recombinant Human Soluble CD4................ 91 

Recombinant Human Sumo-I......................... 92 

Recombinant Human Thrombopoietin............ 91 

Recombinant Human Thyroid Stimulating HO 92 

Recombinant Rubella Virus Capsid C............ 91 

Recombinant Rubella Virus E1 Mosaic ......... 91 

Recombinant Cardiac Roponin I-C................ 92 

Recombinant Toxoplasma GONDII P29 .......... 92 

Recombinant Toxoplasma GONDII P30 .......... 92

Recombinant Treponema Pallidum P15........... 92 




Recombinant Varicella Zoster Virus ORF26.... 91 

Recombinant Varicella Zoster Virus ORF9....... 91 

Recombinant combined Hepatitis C Virus ....... 91 

Removal of KLGH Antibodies.......................... 131 

Removal of serum abundent proteins.............. 51 

Removal of E.coli Contaminant Antibodies...... 131 

Ribavirin........................................................... 103 

Riboflavin, Vitamin B2...................................... 118 

Rifampicin........................................................ 103 

RIPA Buffer ..................................................... 84 

RNAse A.......................................................... 88 

RNAse Inhibitor (Cloned)................................. 89 

RNA-total Arrest............................................... 60 

Rubidium Chloride............................................ 118 

S1 Nuclease.................................................... 88 

Salicylic Acid.................................................... 118 

Salinity Test Kit................................................. 54 

Salmon Gal...................................................... 90 

Salmon-X-Gluca.............................................. 118 

Sambucus nigra Lectin.................................... 27 

Sample Prep Kit For 2-D Gels......................... 138 

SANH.............................................................. 41 

Sarcosine......................................................... 37 

SBA Coated Microtiter Plate............................ 25 

SDS 20% solution............................................ 107 

SDS Buffers..................................................... 68,70,71 

Secondary Amine Micro Plate......................... 23 

Seeband Protein Stain..................................... 48 

Sepharopore 6B........................................... 4 

SFB Linker....................................................... 41 

Silica Beads..................................................... 6 

Silica Coated Magnetic Beads......................... 20 

Silver Nitrate..................................................... 118 

SilverOUT..................................................... 49 

Site-Directed Mutagenesis............................... 139 

Sodium Acetate ............................................... 118 

Sodium Azide................................................... 118 

Sodium Bicarbonate........................................ 118 

Sodium Borate ................................................ 118 

Sodium Carbonate........................................... 118 

Sodium Chloride.............................................. 118 

Sodium Citrate................................................. 76 

Sodium Dodecyl SulfateSDS........................... 107 

Sodium Hydroxide .......................................... 118 

Sodium Iodide................................................. 118 

Sodium Lauroyl Sarcosine SLS....................... 107 

Sodium Metabisulfite....................................... 118 

Sodium Phosphate.......................................... 118 

Sodium Sulfate............................................... 118 

Sorbitol........................................................... 37 

Southern Blot Analysis................................... 140 

Spectinomycin ............................................... 103 

Spermidine..................................................... 118 

SSC Buffer...................................................... 60 

SSC Hybridization Solution............................ 60 

SSPE Buffer................................................... 61 

SSPE Hybridization Solution.......................... 61 

S-tag Antibody................................................ 93 

STE................................................................ 67 

STET ............................................................. 76 

Stop Reagent................................................. 89 

Streptavidin.................................................... 118 

StrepTalon-S............................................... 22 

Streptavidin Coated Microtiter Plate.............. 25 

Streptavidin Sepharose.............................. 16 

Streptomycin Sulfate...................................... 103 

Stripping Buffer ............................................. 50, 80 

Succinic Acid................................................. 119 

Sucrose ........................................................ 119 

Sulfamethoxazole............................................ 103 

Sulfite Test Kit................................................ 55 

Sulfo-BSOCOES............................................ 42 

Sulfo-DST....................................................... 42 

Sulfo-EGS....................................................... 42 

Sulfo-SIAB...................................................... 42 

Sulfo-SMCC.................................................. 41 

Super Broth Powder....................................... 127 

Super Zymolyase........................................... 88 

T4 DNA Ligase.............................................. 88 

T4 Polynucleotide Kinase.............................. 88 

TAE Buffer...................................................... 65 

TAPS.............................................................. 119 

TAQ................................................................ 88 

TB Buffers...................................................... 71 

TBS Buffer..................................................... 80 

TBS-T ........................................................... 80 

TE Buffer........................................................ 68 

TEMED.......................................................... 119 

TES............................................................... 119 

Tetracycline Hydrochloride............................ 109 

Tetradecyl Trimethyl Ammonium Bromide...... 119 

Tetramethylammonium Chloride.................... 119 

TG Buffer....................................................... 71 

TG-SDS Buffer............................................... 71 

Thiamine HCL................................................ 119 

Thiazoyl Blue Tetrazolium Bromide................ 119 

Thidiazuron (TDZ).......................................... 119 

Thimerosal..................................................... 119 

Thiophilic Agarose 4B................................ 12 

Thrombin Buffer............................................. 85 

Ticarcillin........................................................ 103 

Timentin..................................................... 104 

Tissue Blot..................................................... 51 

TM Buffer....................................................... 77 

TMAO............................................................ 36 

TMB............................................................... 90, 94 

TMB, Solution................................................ 90 

TN Buffer....................................................... 77 

TNT Buffer..................................................... 77 

Tobramycin Sulfate........................................ 104 

Total Chlorine Determination Test Kit............ 53 

Total Rapid RNA Purification Kit.................... 60 

TPCK............................................................. 88 

TPCK-treated trypsin..................................... 88 

Transblotting on PVDF................................... 138 

Trichloroacetic Acid........................................ 119 

Tricine Buffer ................................................. 119 

Tricine-SDS Sample Buffer............................ 70 

Trifluralin........................................................ 119 

Trimethoprim.................................................. 119 

Trimethoprim Lactate..................................... 119 

Tris Acetate.................................................... 119 

Tris Base........................................................ 119 

Tris Biotechnology Grade............................... 119 

Tris Buffer....................................................... 68 

Tris Carbonate................................................ 119 

Tris Hydrochloride.......................................... 119 

Tris-CAPS...................................................... 85 

Tris-Glycine Sample Buffer............................ 71 

Tris-HCl ......................................................... 85, 119 

Tris-Phosphate-EDTA ................................... 77 

Tris Tricine Buffer........................................... 71 

Tris Ultra Pure Grade.................................... 119 

Triticum vulgaris Lectin.................................. 27 

Triton Lysis Buffer ......................................... 86 

Triton X100.................................................... 107 

True Blue Protein MW Marker Kit............... 47 

Trypsin........................................................... 89 

Trypsin Digestion Mix.................................... 37 

Tryptone......................................................... 120 

TTC Buffer..................................................... 120 

Tween 20/80.................................................. 107 

Ubiquitin Binding Protein Purification............. 131 

Ubiquitin Sepharose................................... 16 

Ulex europaeus Lectin.................................. 27 

Ultrapure Plasmid Extraction Kit.................... 63 

Unmask Kit................................................. 65 

Uracil.............................................................. 120 

Urea............................................................... 37,120 

Uridine........................................................... 120 

UTP, Uridine-5’-triphosphate......................... 120 

Validamycin A................................................ 104 

Vancomycin HCI............................................ 104 

Vector Construction....................................... 139 

Virginiamycin................................................. 104 

Western Stripper............................................ 38 

Wheat Germ Lectin Micro Plate..................... 25 

Wheat Germ Lectin Sepharopore MB........... 16 

X-Gal.............................................................. 

X-Galactosaminide ....................................... 

X-Glu (Glucopyranoside)............................... 

90 

90 

X-Gluc............................................................ 90 

X-Glucosaminide .......................................... 90 

X-Glucuro....................................................... 90 

Yariv’s Reagent.............................................. 50 

Yeast Extract.................................................. 127 

Yeast Lysis Solution....................................... 68 

Yeast Nitrogen Base...................................... 127 

Yeast Nitrogen Base, Dropouts..................... 127 

Z-competent Transformation Kit..................... 57 

Zeatin............................................................. 120 

Zeocin............................................................ 104 

Zero Binding Microtiter Plates........................ 23 

Zinc Sulfate.................................................... 120 

Zymogram Buffer .......................................... 77 

Copyright 2006 C 

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protein affinity chromatography 

which facilitates purification of 

recombinant and natural proteins. 

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accelerating proteomics and 

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