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ioaffinitymatrix Ligand Coupled Arginine Sepharose This matrix is used to isolate or remove a number of different serine proteases, eg. Prekallikrein, Clostripain and Plasminogen activator from a wide range of materials. L-Arginine is coupled to Sepharose 4B, predominantly through its amino group, leaving the guanidine and carboxyl groups to react reversibly with the target protein. Desorption with ionic gradient or steps. Arginine Separose also has a wide pH and detergent compatibility. • Ligand: L-Arginine • Ligand coupling method: Epoxy • Ligand density: 20-40µmol Arginine/ml medium • Matrix: Agarose 4% Beads Benzamidine Sepharose • Particle size range: 45-165µm • pH stability: 2-12 • Storage: 2-8°C Used in the removal and/or purification of serine proteases, e.g. Thrombin. p-Aminobenzamidine (pABA) immobilized on Sepharose 4B matrix gives high productivity and allows for easy scale-up. Effective in the removal of thrombin and factor Xa after tag cleavage of recombinant proteins. • Ligand: p-Aminobenzamidine (pABA) • Ligand coupling method: Amide • Ligand density: >12µmol p- Aminobenzamidine/ml medium • Binding capacity: >35mg Trypsin/ml • Matrix: Agarose 4% Beads • Particle size range: 45-165µm • pH stability: 2-8°C • Storage: 2-12ºC New Save 25% #506489 10ml $ 74.00 #506490 25ml $ 149.50 #506494 50ml $ 298.00 #506491 10ml $ 87.00 #506492 25ml $ 198.00 #506493 50ml $ 317.00 bioaffinitymatrix Affinity Chromatography activeligands> liveproteins SM www.bio-WORLD.com 1.888.bio.PLUS F: 614.792.8685 save@bio-world.com 9
Affinity Chromatography bioaffinitymatrix Blue Sepharopore CL-6B Dye-ligand affinity matrix for purification of albumin, enzymes binding to NAD + and NADP + , coagulation factors, interferons and related proteins. The ligand, Cibacron Blue, is covalently coupled to Sepharopore through the chlorotriazine ring. Broad spectrum affinity that can be used as a general purpose protein purification tool and specifically used for removal of albumin from serum. Supplied as lyophilized matrix powder (1g swells to approx. 4ml) or as a suspension in 20% ethanol. Equal or superior to Blue Sepharose. • Ligand: Cibacron Blue F3G-A • Ligand coupling method: Triazine coupling • Ligand density: 8µ mol Cibacron Blue/ml • Binding capacity: ≥ 15 mg bovine serum albumin/ml drained gel DEAE Sepharopore • Matrix: Highly cross-linked agarose, 6%Beads • MW exclusion limit: 4 x 10 6 • Particle size range: 52-170µm • pH stability: 3-12 • Coupling conditions: pH 9 - 10, Temp. 4 - 25°C, Time 2-16h • Storage: 2-8°C DEAE bioMatrix is the most widely used chromatographic media for protein purification. BioWorld’s DEAE Sepharopore is prepared by covalent attachment of diethylaminoethyl to Sepharopore CL-6B. DEAE Sepharopore is a weak anion exchanger for binding and resolving of proteins of all pI values. Diethylaminoethyl remains charged over a wide working range of pH 3-9. An excellent matrix for analytical and preparative fractionation of proteins. Supplied as pre swollen in 20% ethanol. Bulk quantity available. • Ligand: diethylaminoethyl • Max flow rate: 150cm/h • Binding capacity: 20-70mg/ml • Matrix: Agarose 6% • Bead size range: 50-170 µm . • pH stability: 3-9 • Storage: 2-8°C #506480 5ml $ 34.50 #506482 25ml $ 107.00 #506484 50ml $ 185.60 #506485 100ml $ 347.00 #506929 50ml $ 80.00 #506930 100ml $ 158.00 #506931 500ml $ 340.00 CH 2CH 3 O CH 2CH 2 N: CH 2CH 3 10 Gelatin Sepharopore Affinity matrix for the purification of fibronectin. Gelatin covalently coupled to Sepharopore 4B by CNBr activation. Supplied as suspension in 0.5M NaCl. • Ligand: Bovine gelatin • Ligand coupling method: CNBr activation • Ligand density: ≥ 5 mg gelatin/ml • Matrix: Agarose, 4% Beads • MW exclusion limit: Approx. 2 x 10 7 • Particle size: 50-170 µm • pH stability: 3–10 • Storage: 2-8°C New New #506908 5ml $ 29.70 #506910 10ml $ 89.50 #506912 25ml $ 98.25
Page 1 and 2: For Current Prices: See the Website
Page 3 and 4: Contact Information 4150 Tuller Roa
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iokits Genomics Plasmid Purificatio
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DNA Supperladder contd. biokits Gen
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iokits Genomics ColonyPCR Kit • T
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uffers&reagents 4 DNA / RNA Electro
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uffers&reagents Electrophoresis TE
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uffers&reagents Electrophoresis SDS
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uffers&reagents Molecular biology 7
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Immunology buffers&reagents Imidazo
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uffers&reagents Horse Blocker • H
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uffers&reagents Protein Purificatio
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E. coli Protein Elution RIPA Buffer
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uffers&reagents Protein Purificatio
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Enzymes & Substrates enzymes&substr
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enzymes&substrates Enzymes & Substr
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Enzymes & Substrate enzymes&substra
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Enzymes & Substrate enzymes&substra
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iochemicals Antibiotics Amphoterici
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Cefotaxime Sodium Chlorhexidine Dig
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Antibiotics biochemicals Hygromycin
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Penicillin (contd.) G, Sterile Phle
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Vancomycin HCl biochemicals Timenti
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io Detergents biochemicals 106 CHAP
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Research Chemicals biochemicals 108
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Research Chemicals biochemicals BES
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2’-Deoxyurdine #705239 1G $ 37.28
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Thiocyanate (Isothiocyanate) #70543
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Sodium Salt #730088 25G $ 24.50 Oxa
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Research Chemicals biochemicals Raf
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Research Chemicals biochemicals Try
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iomedia Animal Animal Cell Culture
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Fetal Calf Bovine Serum • Please
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Animal Cell Culture biomedia MCDB 1
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Plant Tissue & Cell Culture Media b
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customlabservices 9 Affinity Purifi
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Custom Peptide Synthesis customlabs
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Custom Peptide Synthesis customlabs
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Antibody Production Service customl
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customlabservices Recombinant Techn
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customlabservices mRNA Isolation
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D-Fructose.........................
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Recombinant Treponema Pallidum P15.
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