Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
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Orange-G Loading Dye - for molecular biology<br />
Glycerol (6X)<br />
• 6X concentration<br />
• Orange-G (0.25%)<br />
#700824 25ml $ 19.00<br />
• Glycerol in water (18%)<br />
Blue (6X)<br />
#700825 50ml $ 28.50<br />
• Orange-G (0.25%)<br />
• Bromophenol Blue (0.25%)<br />
#700822 10ml $ 19.00<br />
• Xylene Cyanol FF (0.25%)<br />
• Ficoll in water (30%)<br />
• EDTA (30mM)<br />
#700823 25ml $ 34.50<br />
Isoelectrofocusing (IEF) Buffer<br />
Anode (50X), pH 2.4<br />
• Dilute with deionized water<br />
to make 1X concentration.<br />
#743140 500ml $ 16.25<br />
• 1X concentration: PH 2.4 #743141 1L $ 26.75<br />
Phosphoric Acid (7mM)<br />
• Proteases free<br />
Cathode (10X), pH 10.1<br />
• Dilute with deionized water<br />
to make 1X concentration.<br />
#743142 250ml $ 36.25<br />
• 1X concentration: 20mM #743143 500ml $ 62.70<br />
Arginine and 20mM Lysine<br />
Sample Buffer (4X) - for protein electrophoresis<br />
IPG-Strip Equilibration Buffer<br />
• For equilibration of IPG- Strip<br />
before running second<br />
dimension SDS-PAGE.<br />
• No urea-induced protein<br />
carbamylation<br />
• No waste of unused reagents<br />
buffers&reagents<br />
#705940 50ml $ 49.50<br />
#705955 100ml $ 86.60<br />
• Improves resolution and prevents<br />
streaking of protein spots on 2D gels.<br />
Laemmli SDS-Sample Buffer - electrophoretic<br />
dye for denaturation of proteins<br />
Reducing, (4X - 6X)<br />
• 1X solution: SDS (2%), Glycerol<br />
(10%), Tris - HCl (62.5mM),<br />
Bromophenol Blue and<br />
β - mercaptoethanol<br />
• DNase and Protease free<br />
• Stability (20°C) >1 year<br />
Non-reducing, (4X - 6X)<br />
• Ultra Pure Grade<br />
• 1x solution: SDS (2%), Glycerol<br />
(10%), Tris -HCl (62.5mM), and<br />
Bromophenol Blue.<br />
• DNase, RNase and<br />
Protease free<br />
• Stability (20°C) >1 year<br />
• Storage: RT<br />
4X- #742126 50ml $ 28.50<br />
6X- #742114 25ml $ 24.50<br />
• Storage: RT<br />
4X- #742124 25ml $ 19.00<br />
#742118 50ml $ 28.50<br />
6X- #742110 25ml $ 24.50<br />
#742112 50ml $ 39.80<br />
buffers&reagents Electrphoresis<br />
• 20mM Lysine (free base) and<br />
Glycerol (15%)<br />
#742133 25ml $ 19.00<br />
#742134 50ml $ 28.50<br />
MES-SDS Running Buffer (20X)<br />
><br />
accelerate: electrophoresis<br />
• Dilute with deionized water<br />
to make 1X concentration.<br />
• 1X concentration: MES<br />
(50mM), Tris base (50mM),<br />
SDS (0.1%), EDTA (1mM)<br />
• pH 7.3<br />
#743146 500ml $ 34.50<br />
#743147 1L $ 59.70<br />
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69<br />
for protocols visit www.bio-<strong>WORLD</strong>.<strong>com</strong>