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Download bioWORLD Catalog - Bio-WORLD.com

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Orange-G Loading Dye - for molecular biology<br />

Glycerol (6X)<br />

• 6X concentration<br />

• Orange-G (0.25%)<br />

#700824 25ml $ 19.00<br />

• Glycerol in water (18%)<br />

Blue (6X)<br />

#700825 50ml $ 28.50<br />

• Orange-G (0.25%)<br />

• Bromophenol Blue (0.25%)<br />

#700822 10ml $ 19.00<br />

• Xylene Cyanol FF (0.25%)<br />

• Ficoll in water (30%)<br />

• EDTA (30mM)<br />

#700823 25ml $ 34.50<br />

Isoelectrofocusing (IEF) Buffer<br />

Anode (50X), pH 2.4<br />

• Dilute with deionized water<br />

to make 1X concentration.<br />

#743140 500ml $ 16.25<br />

• 1X concentration: PH 2.4 #743141 1L $ 26.75<br />

Phosphoric Acid (7mM)<br />

• Proteases free<br />

Cathode (10X), pH 10.1<br />

• Dilute with deionized water<br />

to make 1X concentration.<br />

#743142 250ml $ 36.25<br />

• 1X concentration: 20mM #743143 500ml $ 62.70<br />

Arginine and 20mM Lysine<br />

Sample Buffer (4X) - for protein electrophoresis<br />

IPG-Strip Equilibration Buffer<br />

• For equilibration of IPG- Strip<br />

before running second<br />

dimension SDS-PAGE.<br />

• No urea-induced protein<br />

carbamylation<br />

• No waste of unused reagents<br />

buffers&reagents<br />

#705940 50ml $ 49.50<br />

#705955 100ml $ 86.60<br />

• Improves resolution and prevents<br />

streaking of protein spots on 2D gels.<br />

Laemmli SDS-Sample Buffer - electrophoretic<br />

dye for denaturation of proteins<br />

Reducing, (4X - 6X)<br />

• 1X solution: SDS (2%), Glycerol<br />

(10%), Tris - HCl (62.5mM),<br />

Bromophenol Blue and<br />

β - mercaptoethanol<br />

• DNase and Protease free<br />

• Stability (20°C) >1 year<br />

Non-reducing, (4X - 6X)<br />

• Ultra Pure Grade<br />

• 1x solution: SDS (2%), Glycerol<br />

(10%), Tris -HCl (62.5mM), and<br />

Bromophenol Blue.<br />

• DNase, RNase and<br />

Protease free<br />

• Stability (20°C) >1 year<br />

• Storage: RT<br />

4X- #742126 50ml $ 28.50<br />

6X- #742114 25ml $ 24.50<br />

• Storage: RT<br />

4X- #742124 25ml $ 19.00<br />

#742118 50ml $ 28.50<br />

6X- #742110 25ml $ 24.50<br />

#742112 50ml $ 39.80<br />

buffers&reagents Electrphoresis<br />

• 20mM Lysine (free base) and<br />

Glycerol (15%)<br />

#742133 25ml $ 19.00<br />

#742134 50ml $ 28.50<br />

MES-SDS Running Buffer (20X)<br />

><br />

accelerate: electrophoresis<br />

• Dilute with deionized water<br />

to make 1X concentration.<br />

• 1X concentration: MES<br />

(50mM), Tris base (50mM),<br />

SDS (0.1%), EDTA (1mM)<br />

• pH 7.3<br />

#743146 500ml $ 34.50<br />

#743147 1L $ 59.70<br />

56<br />

69<br />

for protocols visit www.bio-<strong>WORLD</strong>.<strong>com</strong>

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