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uffers&reagents
Electrophoresis
SDS Sample Loading Buffer
• Standard SDS polyacrylamide
#700958 50ml $ 29.50
electrophoresis sample loading
buffer
#700957 100ml $ 39.70
• 0.05% (w/v) bromophenol blue
• Dilute samples to at least 1:4
with the sample buffer.
#700956 200ml $ 67.50
• Heat samples at 95ºC for 4 minutes prior to loading.
SDS Separating Gel Buffer (4X)
• Standard SDS polyacrylamide
gel 10% electrophoresis
#700952 100ml $ 00.00
separating gel buffer
#700951 250ml $ 00.00
• Buffer in 0.375M Tris, pH 6.8
• 20% (w/v) SDS
#700950 500ml $ 00.00
SDS Stacking Gel Buffer (4X)
• Standard SDS polyacrylamide
gel 10% electrophoresis
separating gel buffer
• Buffer in 0.375M Tris, pH 8.8
• 20% (w/v) SDS
#700954
#700953
#700950
100ml
200ml
500ml
$ 00.00
$ 00.00
$ 00.00
TAE Buffer (Tris Acetate EDTA) - for electrophoresis
Ready-Pack (10X)
• Each Ready-Pack prepares
1L of 10X concentrate.
• pH 8.3 + - 0.1
Ready-Pack (25X)
• Each pack makes 1L of 25X
• 1X contains: Tris (0.04M ),
Acetate (0.04M ), EDTA
(0.001M)
• pH at 25C: 8.2 - 8.4
• (P/F): Pass
• Storage: RT
Liquid Concentrate (25X)
• 1X contains: Acetate (0.04M),
EDTA (0.001M )
• pH 8.3 + - 0.1
• Storage: RT
#730125 1x1L/pk $ 14.95
#730127 10x1L/pk $ 56.35
#730128 2x1L/pk $ 10.25
#730137 1L /pk $ 39.77
#730131 2x1L/pk $ 59.50
#730135 10x1L/pk $ 170.2
#730129 1x40L/pk $ 59.50
#730144 1L $ 39.50
#730134 4L $ 78.00
Liquid Concentrate (50X)
Tris - for protein analysis
Tricine-SDS Running Buffer (10X)
• Dilute with deionized water
#743150 500ml $ 28.50
• 1X contains: Tris base (50mM),
Tricine (50mM), SDS (3.5mM)
#743151 1L $ 48.50
pH 8.24
Sample Buffer (4X)
• Same as above
• Each pack makes 50X of stated
volume
#730136 500ml $ 23.70
#730138 1L $ 38.40
#730140 4L $ 97.60
• Tris-HCl 400mM, Glycerol (40%)
• Bromophenol Blue (0.02%)
• pH 8.6
#742106 25ml $ 21.00
#742108 50ml $ 31.50
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accelerate: electrophoresis