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Enzymes & Substrates enzymes&substrates<br />
enzymes&substrates<br />
Enzymes<br />
Alkaline Phosphatase<br />
with Glycerol<br />
Cellulase (Onozuka R-10)<br />
for protoplast preparation<br />
• Often used in <strong>com</strong>bination with Macerozyme R-10<br />
a-Chymotrypsin<br />
• Cleaves peptides on the C-terminus side of aromatic<br />
residues<br />
DNAse 1 (Bovine Pancreas)<br />
• CAS: 9003-98-9<br />
Galactosidase<br />
• Catalyzes the cleavage of beta-linked terminal<br />
galactose-containing substrates.<br />
Glucose Oxidase<br />
• Capable of oxidizing D-aldohexoses, monodeoxy-Dglucoses,<br />
and methyl-D-glucoses at varying rates.<br />
Horseradish Peroxidase<br />
• Horseradish Peroxidase is a single chain polypeptide<br />
containing four disulfide bridges and belongs to the<br />
ferroprotoporphyrin group of peroxidases.<br />
#752025 1ku $ 44.20<br />
#752027 2.5ku $ 94.60<br />
#752029 5ku $ 170.10<br />
#752021 1ku $ 35.60<br />
#752023 5ku $ 64.60<br />
#705117 1G $ 49.50<br />
#705119 5G $ 169.00<br />
#750033 1G $ 44.10<br />
#750035 5G $ 194.30<br />
#750048 50ku $ 119.70<br />
#750050 100ku $ 241.75<br />
#750137 1ku $ 34.70<br />
#750139 5ku $ 168.00<br />
#505168 up to 10mg $ 875.00<br />
#750057 50ku $ 17.00<br />
#750059 100ku $ 29.20<br />
#750061 500ku $ 116.60<br />
#750081 10ku $ 49.60<br />
#705797 25ku $ 64.20<br />
#705798 100ku $ 120.47<br />
Pectinase • Used in plant protoplast preparation<br />
to digest cell wall prior to organelle isolation.<br />
Pepsin 1:3000 (Porcine Source)<br />
• Preferentially cleaves C-terminal to Phenylalanine,<br />
Leucine and Glutamic Acid.<br />
Protein A<br />
Proteinase K<br />
• A serine proteinase used to inactivate RNAse and<br />
DNAse in RNA and DNA isolation protcols<br />
Solution 20mg/ml<br />
RNAse A<br />
• RNase A specifically hydrolyzes RNA between the<br />
3’-phosphate of a pyridine ribonucleotide and the 5’-<br />
hydroxyl of the adjacent nucleotide.<br />
S1 Nuclease<br />
Super Zymolyase<br />
T4 DNA Ligase<br />
FirePol<br />
Hot Fire Pol (TAQ)<br />
#705099 10G $ 72.20<br />
#705796 250G $ 34.65<br />
#705800 500G $ 63.36<br />
#700800 5mg $ 46.50<br />
#700802 20mg $ 150.00<br />
#700804 100mg $ 650.00<br />
#705873 100mg $ 72.24<br />
#705874 500mg $ 279.90<br />
#705875 1G $ 549.33<br />
#750085 5ml $ 85.50<br />
#750089 25ml $ 338.10<br />
#750092 250mg $ 112.20<br />
#750094 500mg $ 189.10<br />
#750096 1G $ 353.40<br />
#752104 100ku $ 209.00<br />
#513020 2x0.5ml $ 47.70<br />
#752176 20ku $ 47.50<br />
#752177 100ku $ 190.00<br />
#516222 1000u $ 79.00<br />
#516227 4000u $ 359.00<br />
#516220 1000u $ 99.00<br />
#516226 4000u $ 386.00<br />
en<br />
Lysozyme<br />
• Used in purification protocols of genomic and plas<br />
mid DNA to lyse bacterial cells.<br />
#750073 5G $ 24.75<br />
#750075 10G $ 35.20<br />
T4 Polynucleotide Kinase<br />
• Catalyzes the transfer of the g phosphate of ATP to<br />
a 5’-OH terminus in either RNA or DNA.<br />
#752179 0.5ku $ 23.75<br />
#752178 2.5ku $ 90.25<br />
88<br />
Macerozyme R-10<br />
• Used in plant protoplast preparation to digest cell<br />
wall prior to organelle isolation.<br />
Malate Dehydrogenase<br />
• Catalyzes the following reaction: Oxaloacetate + ß-<br />
NADH - L-Malate + ß-NAD<br />
#750076 25G $ 59.00<br />
#750082 100G $ 147.00<br />
#750080 1G $ 49.00<br />
#750084 5G $ 129.75<br />
#750077 25ku $ 38.40<br />
Tosyl Phenylalanyl<br />
Chloromethyl Ketone (TPCK)<br />
• MF: C 14 H 21 ClN 2 O 3 S HCl<br />
• MW: 369.31<br />
TPCK-Treated Trypsin<br />
#750120 50mg $ 9.00<br />
#750121 100mg $ 15.00<br />
#750122 250mg $ 35.00<br />
#750114 50mg $ 27.95<br />
#750115 100mg $ 45.50<br />
For In vitro lab use only - Not for human use or consumption
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