Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
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E. coli Protein Elution<br />
RIPA Buffer - for extracting proteins from cells<br />
buffers&reagents<br />
Protein Purification<br />
• Na 2 HPO 4 (50mM)<br />
#700212 250ml $ 19.50<br />
• NaCl (300mM)<br />
• Imidazol (250mM), pH 8.0<br />
#700213 500ml $ 35.00<br />
• For His-tag protein purification from E. coli & insect cells<br />
Mammalian Protein Elution<br />
• Na 2 HPO 4 (50mM)<br />
• NaCl (300mM)<br />
#700217 250ml $ 19.50<br />
• Imidazol (250mM)<br />
#700218 500ml $ 35.00<br />
• Tween 20 (0.5%), pH 8.0<br />
• For His-tag protein purification from mammalian cells<br />
Mammalian Protein Wash<br />
• Na 2 HPO 4 (50mM)<br />
#700219 250ml $ 19.50<br />
• NaCl (300mM)<br />
• Imidazol (20mM)<br />
#700220 500ml $ 35.00<br />
• Tween 20 (0.5%), pH 8.0<br />
• For HIS-TAG protein purification from mammalian cells<br />
Protein Coupling Kit<br />
RIPA pH 7.4<br />
• Tris-HCl (100mM)<br />
• NaCl (300mM)<br />
• NP-40 (2%)<br />
• Sodium Deoxycholate (1.0%)<br />
• SDS (0.2%)<br />
RIPA pH 8.0 (1X)<br />
• Tris-HCl (50mM)<br />
• NaCl (150mM)<br />
• NP-40 (1%)<br />
• Sodium Deoxycholate (0.5%)<br />
• SDS (0.1%)<br />
RIPA with EDTA (1X)<br />
• Tris-HCl (50mM)<br />
• NaCl (150mM)<br />
• NP-40 (1%)<br />
• Sodium Deoxycholate (0.5%)<br />
• SDS (0.1%)<br />
1X- #765512 250ml $ 29.70<br />
#765513 500ml $ 49.50<br />
2X- #765504 250ml $ 27.50<br />
#765505 500ml $ 44.70<br />
#765502 250ml $ 29.70<br />
#765503 500ml $ 49.50<br />
• EGTA (1mM) / EDTA (5mM)<br />
#765506 250ml $ 29.70<br />
#765507 500ml $ 49.50<br />
• EGTA (5mM)<br />
• pH 7.4<br />
• Gel swelling solution:<br />
#730265 1 Kit $ 93.00<br />
HCl (1mM)<br />
• Gel coupling solution-Sodium Borate or Citrate (100mM),<br />
NaCl (500mM), pH 9.3<br />
RIPA with EDTA & EGTA<br />
• Tris-HCl (50mM)<br />
• NaCl (150mM)<br />
• NP-40 (1%)<br />
#765510 250ml $ 29.70<br />
#765511 500ml $ 49.50<br />
• Gel washing solution-Sodium Acetate (100mM),<br />
• Sodium Deoxycholate (0.5%)<br />
NaCl (500mM),pH 4.0<br />
• SDS (0.1%)<br />
• Kit is used for CnBr-activated Sepharose / Sepharophore<br />
• EGTA (1mM) / EDTA (5mM)<br />
Coupling.<br />
RIPA with Triton X-100<br />
• 1X - Tris-HCl (50mM), pH 7.4,<br />
NaCl (150mM), Triton X-100<br />
(1%), Sodium Deoxycholate<br />
1X- #765516 250ml $ 29.70<br />
#765517 500ml $ 49.50<br />
(0.5%), SDS (0.1%)<br />
• 5X - Tris-HCl (250mM), pH 7.4,<br />
NaCl (750mM), Triton X-100<br />
5X- #765514 250ml $ 29.70<br />
#765515 500ml $ 57.25<br />
(5%), Sodium Deoxycholate<br />
(2.5%), SDS (0.5%)<br />
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