Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
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Streptavidin- Sepharose<br />
bioaffinitymatrix<br />
Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. For binding biotinylated<br />
<strong>com</strong>pounds. Supplied as buffered saline solution<br />
• Ligand: Native streptavidin (from<br />
Streptomyces avidinii)<br />
• Ligand coupling method: CNBr activation<br />
• Ligand density: 2 mg/ml<br />
• Binding capacity: ≥ 20 µg biotin/ml<br />
drained gel<br />
• Matrix: Agarose, 4% Beads<br />
• MW exclusion limit: Approx. 2 x 10 7<br />
• Particle size range: 45-165µm<br />
• pH stability: 3-10<br />
• Elution buffer: 50 mM<br />
ammonium acetate, 0.5 M<br />
NaCl, pH 4.0 (provided)<br />
• Storage: 2-8°C<br />
#506914 1ml $ 54.00<br />
#506916 10ml $ 379.00<br />
Wheat Germ Lectin Sepharopore-MB<br />
Affinity Chromatography<br />
Wheat Germ Lectin (agglutinin) binds specifically to cells, subcellular particles, glycoconjugates and polysaccharides containing<br />
N-acetylglucosamine residues. In WGA- 6MB, the lectin is covalently coupled to Sepharopore 6MB. This affinity<br />
adsorbent is useful for the general fractionation of glycoconjugates, but the large bead size also facilitates the specific<br />
binding of cells with practically no physical entrapment of the cells. WGA Sepharopore 6MB is supplied in 0.9% NaCl<br />
containing 0.04% sodium azide as a preservative.<br />
• Binding Specificity: Depends on the <strong>com</strong>position<br />
and structure of the glycoconjugate or<br />
polysaccharide<br />
• Ligand coupling method: CNBr activation<br />
• Ligand Concentration: 5mg Wheat germ lectin/<br />
ml drained gel<br />
• Matrix: Agarose, 6% Macrobeads<br />
• Exclusion limit (Mr): 40 megadaltons<br />
• Bead size range: 250µm-400µm diameter<br />
• Ligand source: Triticum vulgaris<br />
• pH stability: 4–9<br />
• Storage: 4-7°C (Do not freeze)<br />
#506963 1ml $ 88.00<br />
#506964 5ml $ 187.00<br />
#506965 10ml $ 276.00<br />
#506961 25ml $ 398.00<br />
CH 2CH 3<br />
WGA<br />
O CH 2CH 2<br />
N:<br />
CH 2CH 3<br />
New<br />
Ubiquitin-Sepharose<br />
Covalently linked ubiquitin to Sepharose beads via primary amine coupling leaving the C-terminus free. Useful for<br />
binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3’s) and ubiquitinterminal<br />
hydrolyases. (ucms)<br />
#506451 2x1ml $ 96.00<br />
#506446 10ml $ 195.00<br />
• Ligand: Red blood cell ubiquitin<br />
• Ligand coupling method: CNBr<br />
• Matrix: Agarose, 4% Beads<br />
• Binding capacity: 2mg/ml resin<br />
• Storage 4ºC<br />
• Supplied: in 20% ethanol solution.<br />
Study<br />
ubiquitinylation<br />
and proteasome<br />
pathways!<br />
New<br />
16
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