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Download bioWORLD Catalog - Bio-WORLD.com

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iokits<br />

Genomics<br />

Plasmid Purification Powerprep Kit<br />

• High quality plasmid purification based on alkaline lysis.<br />

• Plasmid is allowed to bind to PinkResin, a binding matrix, followed by<br />

quick washing steps & elution of plasmids using spin columns.<br />

• Suitable for restriction enzyme analysis, fluorescent & radioactive<br />

sequencing, subcloning, transfection & in vitro transcription.<br />

• Mini yields from 1.5ml culture, Large from 50-100ml culture.<br />

Mini<br />

#515985 50 preps $ 82.75<br />

#515986 100 preps $ 148.25<br />

Large #515987 10 preps $ 105.80<br />

#515988 20 preps $ 199.25<br />

Rapid RNA Purification Kit<br />

• <strong>Bio</strong>technology Grade<br />

• Total RNA<br />

#705881 1 Kit $ 107.10<br />

• Reagents included in kit: Reagent 1, Tissue Homogenization<br />

Suspension, Reagent 2, Reagent 3, Reagent 4, RNA Precipitation<br />

Reagent & Homogenization Pestles.<br />

• Storage: RT<br />

RNA-Total Arrest<br />

• Optimized mixture of<br />

#535101 10 preps $ 37.40<br />

chaotropic agents & RNase<br />

inhibitors which eliminates #535100 50 preps $ 129.95<br />

RNase activity in 5 seconds & is universally <strong>com</strong>patible with phenol,<br />

chloroform & other detergent-based RNA purification protocols.<br />

• Contains enough reagent to isolate RNA from 10 grams of tissue or 200<br />

preps of 50mg tissue each.<br />

• Permits extremely rapid isolation of total RNA from cells, regardless of<br />

origin & is free of DNA & protein.<br />

Southern and Northern blots Hybridization<br />

Saline Sodium Citrate (20X)<br />

• Ultra Pure Grade<br />

#730296 2x1L/ready pk $ 27.57<br />

• Chloride content (1% water)<br />

(mg/L) 110-120<br />

#730298 1L lq concentrate $ 27.50<br />

• pH (1% water) at 25°C: 7.85-<br />

8.15<br />

• Hazard: Irritant<br />

• Storage: RT<br />

Prehybridization Solution<br />

• SSC 6X<br />

• Denhartdt’s solution 5X<br />

• SDS (0.5%)<br />

• Denatured, phenol-chloroform<br />

extracted from salmon DNA (100µg/ml)<br />

Hybridization Solution<br />

• SSC 6X<br />

• Blotto (5%)<br />

BIOzol Plant RNA Isolation Kit<br />

New<br />

#730299 2L lq concentrate $ 49.70<br />

- hybridization of probes to nucleic acids<br />

#700830 50ml $ 46.20<br />

#700831 100ml $ 89.20<br />

- hybridization of probes to nucleic acids<br />

#700834 100ml $ 34.50<br />

• BIOzol Kit is a single phase<br />

#527700 1 Kit $ 98.50<br />

solution designed specifically<br />

for the isolation of pure, intact RNA from tissue and cells in less than<br />

90 minutes.<br />

• Kit utilizes guanidine thiocyanate and ß-mercaptoethanol, two of the<br />

most potent inhibitors of RNase, to effectively disrupt cellular<br />

structures while inactivating RNase released from membrane-bound<br />

organelles.<br />

• Total RNA of free protein, and DNA and can be used for cDNA synthesis,<br />

Northern blotting and in vitro translation.<br />

• Procedure eliminates the need for lengthy ethanol precipitations and<br />

cesium chloride gradients.<br />

• One kit performs 100 RNA isolations (each from 100 milligrams of<br />

tissue) Product can be used as a cost effective substitute to TRIzol<br />

60<br />

Wearing gloves is highly re<strong>com</strong>mended when handling kit contents

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