Download bioWORLD Catalog - Bio-WORLD.com

uffers&reagents
Protein Purification
EIA Buffer (10X) Concentrate - makes 500ml of 1X
solution when diluted with distilled water
• Solution contains 1% BSA,
#730139 50ml $ 24.40
4M NaCl, 10mM EDTA and
0.1% Azide
• 1M Phosphate Solution
• pH 7.4
#730190 100ml $ 37.00
• Stability >1 year at 4°C
HEPES Buffer & Reagents
HEPES 1M Sterile pH 7.3
• Biotechnology Grade
• MF: C 18 H 18 N 2 O 4 S
#730048 100ml $ 18.27
• DNAse, RNase, Protease free
• pH (at 37°C) 7.2 - 7.4
• Storage: RT
#730050 500ml $ 47.35
• For HEPES Chemical (see page 106)
HEPES Buffered Saline (2X) - for biological sciences
• NaCl (280mM)
• KCl (10mM)
• Na 2 HPO 4 (1.5mM)
• Dextrose (12mM)
• HEPES (50mM)
• pH 7.01
Protein Extraction (5X)
• HEPES (250mM)
• CaCl 2 (5mM)
• pH 7.4
• Add protease inhibitor according to your protocol.
#700620 250ml $ 28.50
#700621 500ml $ 49.70
- for extracting proteins from cells
#765535 250ml $ 23.75
#765536 500ml $ 37.50
Gelatin (10%) - to block non specific binding site for antibodies on
the membrane
• Ready to use
• Gelatin (10%) solution
• For fish gelatin (see page 77)
MES Buffer - used in plant cell culture
• MF: C 6 H 13 NO 4 S
• MW: 195.2
• CAS: 4432-31-9
• Ultra Pure Grade
• pH range 5.5 - 6.7
• pKa at 25°C 6.10 ( + -0.05)
• 1M Solution
• Storage: RT
• pKa value near physiological
pH- not absorbed through cell membranes.
• Essentially transparent to UV light
• For MES Chemical (see page 107)
#740096 500ml $ 33.25
#740097 1L $ 57.00
pH
#700628 6.1 250ml $ 23.75
#700629 6.1 500ml $ 39.90
#700631 6.5 250ml $ 23.75
#700632 6.5 500ml $ 39.90
©
Triton Protein Extraction
• HEPES (50mM)
• Triton X-100 (10%)
• pH 7.4
• Add protease inhibitor according
to your protocol
#765537 250ml $ 23.75
#765538 500ml $ 38.00
82