Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
Download bioWORLD Catalog - Bio-WORLD.com
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
uffers&reagents<br />
Protein Purification<br />
Protein Extraction (5X)<br />
• Tris-HCl (0.5M)<br />
• β-Mercaptoethanol (100mM)<br />
• EDTA (20mM)<br />
• pH 8.0<br />
• Add protease inhibitor according to your protocol<br />
Tris HCl (Sterile)<br />
• <strong>Bio</strong>technology Grade<br />
• Storage: RT<br />
• Hazard: Irritant<br />
* M = Molarity<br />
#765533 250ml $ 23.75<br />
#765534 500ml $ 38.00<br />
* M<br />
pH<br />
#730214 0.5 6.8 500ml $ 29.72<br />
#730206 0.1 7.4 100ml $ 17.43<br />
#730218 0.1 7.4 500ml $ 27.51<br />
#730199 1 7.5 100ml $ 23.52<br />
#730195 1 7.5 500ml $ 41.50<br />
#730212 2 7.5 1L $ 87.84<br />
#730213 2 7.8 500ml $ 44.85<br />
#730194 1 8.0 100ml $ 16.38<br />
#730197 1 8.5 500ml $ 18.27<br />
DTT (2M)<br />
• Ultrapure DTT for<br />
protein reduction.<br />
• Add an aliquot in buffer<br />
solution to maintain protein<br />
reduction potential.<br />
• Protease free for proteomics<br />
work. For solid DTT see <strong>Catalog</strong># 705323, page112)<br />
#515939 5 x 1ml $ 37.40<br />
#515929 10 x 1ml $ 49.00<br />
#730198 1 9.0 250ml $ 21.48<br />
#730216 1 10 250ml $ 19.53<br />
Triton Cell Lysis Buffer - for extracting proteins from cells<br />
• 1X concentration<br />
• Tris-HCl (50mM)<br />
• NaCl (150mM)<br />
• Triton X-100 (1%)<br />
• EDTA (5mM)<br />
pH<br />
#765518 7.4 250ml $ 19.70<br />
#765519 7.4 500ml $ 34.50<br />
#765521 8.0 250ml $ 19.70<br />
#765522 8.0 500ml $ 29.70<br />
86<br />
><br />
accelerate: proteinpurification
Change language