Lab #6 - Cellular Respiration

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Exercise 2 – Detecting cellular respiration in a mitochondrial suspension 1. Review the experiment below, write your hypothesis on your worksheet and identify the independent and dependent variables as well as the control. 2. Label 3 test tubes and add the components indicated in the chart below, in order: Tube #1 Tube #2 Tube #3 Phosphate Buffer Solution 4.0 ml 3.7 ml 3.4 ml DCPIP 0.5 ml 0.5 ml 0.5 ml Succinate Solution 0 ml 0.3 ml 0.6 ml Lima Bean Extract 0.5 ml 0.5 ml 0.5 ml TOTAL 5.0 ml 5.0 ml 5.0 ml 3. Make sure each tube is mixed and score the color of each tube every 5 minutes for a total of 30 minutes using the scale shown below: 0 1 2 3 4 5 4. Graph color score vs time for each tube and answer the associated questions. Part 3: DESIGNING AN EXPERIMENT Having investigated alcohol fermentation in yeast and cellular respiration in a mitochondrial suspension, you and your group will design and carry out a new experiment to expand on what you have already learned. Exercise 3 – Design an experiment 1. Decide as a group to further investigate yeast fermentation or cellular respiration in lima bean mitochondrial suspension. 2. Identify an independent variable you have not already investigated (e.g., amount of corn syrup or mitochondrial suspension) and come up with a hypothesis with regard to this variable. Write the hypothesis on your worksheet. 3. Design an experiment to test this hypothesis. On your worksheet, briefly describe your experimental plan, and identify the independent variable, dependent variable and control. 4. Carry out your experiment, record and graph the results on your worksheet, and write your conclusion.
Laboratory 6 worksheet – Fermentation & Cellular Respiration Name: ____________________________ Group: ________ Exercise 1 – Yeast fermentation Date: _______________ Start time: _________End Time: ________ State your hypothesis below and identify the indicated components of this experiment: Hypothesis: Independent variable: Dependent variable: Control: Results: Tube 1 Tube 2 Tube 3 0 min 5 min 10 min 15 min 20 min 25 min 30 min On the grid below, graph the results for each tube by plotting the amount of gas produced vs time. Did these results support your hypothesis? Explain.
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Lab #6 - Cellular Respiration

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