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<strong>LAB</strong>ORATORY 5 WORKSHEET<br />
Ex. 1 – Digestion of cream by lipase<br />
Your control tubes are the tubes without lipase. Why is it<br />
important to include the second control (tube #2)?<br />
Which of your reactions was most acidic?<br />
What pH indicator allowed you to observe changes in pH?<br />
Name ________________________<br />
Section_______________________<br />
tube # color<br />
1<br />
2<br />
3<br />
4<br />
What product caused the pH to drop in the acidic reactions?<br />
Did bile alone (without lipase) result in triglyceride digestion?<br />
Why or why not?<br />
Did bile aid the digestion of triglycerides in the cream? If so, what is its role in this reaction?<br />
Ex. 2 – Digestion of gelatin by trypsin<br />
State your hypothesis for this experiment and identify the control.<br />
Which of your reactions, if any, remained liquid after incubating on ice? ___________________<br />
Explain your results below:<br />
Human beings can make only 12 of the 20 amino acids needed to build proteins, the other 8 are<br />
referred to as essential amino acids since they can only be obtained from food. Gelatin is<br />
mostly collagen extracted from animal connective tissues and contains little to none of the<br />
essential amino acids methionine and tryptophan. Is gelatin by itself a nutritious protein source?<br />
Why or why not?
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