02 BOOK OF ABSTRACTS .indd

Correlation of chromosomal abnormalities and results
of microsatellite markers analysis in radiation induced
T-cell lymphomas in mice
J. Piskorowska 1 , H. Szymanska 1 , E. Krysiak 1 , M. Gajewska 1 ,
B. Pienkowska-Grela 3 , H. Skurzak 2 , P. Demant 4 , A. Czarnomska 1
1
Department of Genetics and Laboratory Animal Breeding, The Maria Sklodowska -
Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland; 2 Department
of Immunology, The Maria Sklodowska - Curie Memorial Cancer Center and Institute
of Oncology, Warsaw, Poland; 3 Cytogenetic Laboratory, The Maria Sklodowska - Curie
Memorial Cancer Center and Institute of Oncology, Warsaw, Poland; 4 Department of
Cellular and Molecular Biology, Roswell Park Cancer Institute, Buffalo, N.Y., U.S.A.
The aim of the presented investigations was to map the genes responsible for the
development of radiation induced T-cell lymphoma in recombinant congenic mouse
strains (RCS) CcS/Dem. The tumors were histopathologically and immunologically
classified. Morphology of mouse lymphomas was evaluated by microscopic
examination. Estimating the phenotype of lymphomas was based on flow cytometry
(FCM) analysis. The lymphomas were classified according to “Bethesda proposals
for classification of lymphoid neoplasms in mice” (2002).
In order to map the genes responsible for radiation-induced T-cell lymphomas the
neoplasms representing two from seven found immunophenotypes were selected:
CD90 + (CD4/CD8) + sCD3 + and CD90 + (CD4/CD8) + sCD3 - . The microsatellite analysis
was performed using the PCR amplification method. Genomic DNA was obtained
from the tail of mice. DNA of all animals with tumor was characterized for the 24
microsatellite loci. The PCR amplification was performed as described by Dietrich
et al.(1992).
The genetic linkage has been evaluated using the SAS package, and the significance
of the observed linkages has been corrected for whole genome screening as
described by Lander and Kruglyak (1996). The obtained results suggested linkage of
the genes responsible for radiation-induced T-cell lymphomas in CcS mice involving
chromosomes 10 (D10 Mit134) and 16 (D16 Mit34).
Cytogenetic analysis of these tumors was performed according to the standard
procedure. G-bands were obtained with Wright stain. The karyotypes were described
according to the International Committee on Standardized Genetic Nomenclature for
Mice: Rules for nomenclature of chromosome aberrations (2005) and documented
with the Lucia imaging system. The analysis of the found non random chromosome
abnormalities - translocations and metacentric translocations - seems to confirm the
results obtained by microsatellite investigations.
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