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Visible light microscopy, efficient use of techniques and<br />
solutions to common complications<br />
Matja` Mencej<br />
Olympus Slovenija d.o.o.; In cooperation with dr. Rainer Wegerhoff, Olympus Life and<br />
Material Science Europe GmBH<br />
Historical overview<br />
Olympus entered the world of microscopy in 1912. Today Olympus is the leading<br />
manufacturer of microscopic equipment. The range of products used in the area<br />
of life science as well as material science is amongst the largest in the world. In<br />
Slovenia there are over 2000 installed Olympus systems, which range from the<br />
simplest school microscope to motorized research systems. In Europe we are also<br />
amongst the leaders in the area of confocal microscopes.<br />
Optics<br />
l32<br />
49<br />
Top of the line optics with the basic systems, as well with the research systems<br />
enables the best possible illumination of the field of view and homogenous field<br />
of view in the whole range of products. The introduction of UIS (Universal Infinity<br />
System) was a large step towards our goal, that is the best possible ergonomics for<br />
the user of the microscopy system. The difference between the UIS and previous<br />
system will be presented in the presentation.<br />
To be able to understand why we have a picture in the microscope we need a bit of<br />
physics. Light has dual nature, it can be modeled as waves and as particles (quanta).<br />
For the purposes of this presentation we will choose the wave model of light.<br />
Daylight is composed of a palette fo wavelengths. The human eye is able to detect<br />
only a small fraction of the whole spectrum of electromagnetic radiation. Picture 1.<br />
Picture 1: Spectrum of visible light.
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