APPENDIX I Toxicity Identification Evaluation Reports for Chollas ...

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Toxicity Identification Evaluation of Sweetwater River Stormwater Using Selenastrum capricornutum August 2006 TABLE OF CONTENTS 1. EXECUTIVE SUMMARY ..................................................................................................1 2. INTRODUCTION...............................................................................................................3 2.1 Toxicity Identification Evaluation (TIE) Testing..........................................4 2.2 Initial Toxicity Testing Summary for Sweetwater Stormwater....................5 3. MATERIALS AND METHODS ..........................................................................................5 3.1 Test Procedures ........................................................................................5 3.1.1 Toxicity Test Using Selenastrum capricornutum ....................................... 5 3.2 Test Solution Preparation ..........................................................................6 3.3 Water Quality.............................................................................................6 3.4 Sample Receipt .........................................................................................6 3.5 Phase I TIE Methods .................................................................................6 3.5.1 Protocol Modifications................................................................................ 6 3.5.2 Baseline Tests ........................................................................................... 6 3.5.3 Ethylenediaminetetraacetic Acid (EDTA) Tests......................................... 6 3.5.4 Filtration Tests ........................................................................................... 7 3.5.5 C 18 Solid Phase Extraction and Methanol Add-Back Tests ....................... 7 3.5.6 Piperonyl Butoxide (PBO)Tests................................................................. 7 3.6 Statistical Analysis.....................................................................................7 4. RESULTS..........................................................................................................................8 4.1 Sweetwater River Selenastrum capricornutum..........................................8 4.1.1 Results of TIE performed on October 18, 2005 Sample............................ 8 4.1.2 Summary of TIE Performed on October 18, 2005 Sample ...................... 10 4.1.3 Statistical Analysis: Differences in Growth Among Stormwater Dilutions................................................................................................... 10 5. DISCUSSION..................................................................................................................11 6. REFERENCES................................................................................................................13 LIST OF TABLES Table 1. Triad definitions for San Diego Storm Water Monitoring Program.................................3 Table 2: Toxicity Identification Evaluation Procedures .................................................................4 Table 3: Summary of Growth in TIE tests on Sweetwater Stormwater Sample Collected on October 18, 2005.................................................................................................................10 LIST OF FIGURES Figure 1. Growth of S. Capricornutum exposed to multiple dilutions of a Sweetwater River stormwater sample. Different letters indicate statistically significant differences among treatments where B>C>A>D................................................................................................10 Weston Solutions, Inc. i
Toxicity Identification Evaluation of Sweetwater River Stormwater Using Selenastrum capricornutum August 2006 ACRONYMS AND ABBREVIATIONS °C Degrees Celsius EDTA Ethylenediaminetetraacetic Acid g Grams > Greater Than < Less Than L Liter LADPW Los Angeles Department of Public Works MeOH Methanol µL Microliter mg/L Milligram Per Liter mL Milliliter M Molar MLS Mass Loading Station NOEC No Observed Effect Concentration PBO Piperonyl butoxide ppt Parts Per Thousand % Percent KCl Potassium Chloride K ow Octanol – Water Partition Coefficient STS Sodium Thiosulfate SPE Solid Phase Extraction TSS Total Suspended Solids TDS Total Dissolved Solids TUc Toxic Unit Chronic = 100/NOEC USEPA United States Environmental Protection Agency Weston Weston Solutions, Inc. Weston Solutions, Inc. ii
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APPENDIX I Toxicity Identification Evaluation Reports for Chollas ...

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