APPENDIX I Toxicity Identification Evaluation Reports for Chollas ...

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Toxicity Identification Evaluation of Chollas Creek Stormwater using Hyalella azteca Table of Contents August 2006 TABLE OF CONTENTS 1. Executive Summary ..........................................................................................................1 2. INTRODUCTION...............................................................................................................4 2.1 Background and History ............................................................................4 2.2 Toxicity Identification Evaluation (TIE) Testing..........................................5 2.3 Initial Toxicity Testing Summary For Chollas Creek Stormwater...............7 3. MATERIALS AND METHODS ..........................................................................................8 3.1 Test Procedures ........................................................................................8 3.1.1 Toxicity Test Using Hyalella azteca........................................................... 8 3.2 Test Solution Preparation ..........................................................................8 3.3 Water Quality.............................................................................................8 3.4 Sample Receipt .........................................................................................8 3.5 Phase I TIE Methods .................................................................................9 3.5.1 Protocol Modifications................................................................................ 9 3.5.2 Baseline Tests ........................................................................................... 9 3.5.3 Ethylenediaminetetraacetic Acid (EDTA) Tests......................................... 9 3.5.4 Sodium Thiosulfate (STS) Tests.............................................................. 10 3.5.5 Aeration Tests.......................................................................................... 10 3.5.6 Filtration Tests ......................................................................................... 11 3.5.7 C 18 Solid Phase Extraction and Methanol Add-Back Tests ..................... 11 3.5.8 Graduated pH Tests ................................................................................ 12 3.5.9 Piperonyl Butoxide (PBO) Tests.............................................................. 12 3.5.10 Carboxyl Esterase Tests.......................................................................... 12 3.6 Statistical Analysis...................................................................................13 4. Results ............................................................................................................................14 4.1 TIE Tests on Chollas Creek Stormwater Using Hyalella azteca..............14 4.1.1 Results of TIE Performed on the October 18, 2005 Sample ................... 14 4.1.2 Summary of TIE Performed on the October 18, 2005 Stormwater Sample .................................................................................................... 16 4.1.3 Results of Special Testing Performed on November 11, 2005 Sample .................................................................................................... 17 4.1.4 Summary of Specialized Testing Performed on November 11, 2005 Sample .................................................................................................... 18 4.1.5 Results of Phase I TIE Performed on January 2, 2006 Sample .............. 18 4.1.6 Summary of TIE performed on January 2, 2006 Sample ........................ 20 4.1.7 Results of Phase I TIE Performed on February 19, 2006 Sample........... 22 4.1.8 Summary of TIE performed on February 19, 2006 Sample..................... 24 4.2 Chemical Analyses of Chollas Creek Stormwater ...................................24 5. DISCUSSION..................................................................................................................28 6. REFERENCES................................................................................................................31 Weston Solutions, Inc. i
Toxicity Identification Evaluation of Chollas Creek Stormwater using Hyalella azteca Table of Contents August 2006 LIST OF TABLES Table 1. Triad Definitions for San Diego Storm Water Monitoring Program. ................................4 Table 2. Toxicity Identification Evaluation (TIE) Manipulations.....................................................6 Table 3. Percent Survival of Hyalella azteca in TIE Tests performed on the Chollas Creek Stormwater Sample Collected on October 18, 2005. ..........................................................14 Table 4. Percent Survival of Hyalella azteca in Special Tests performed on the Chollas Creek Stormwater Sample Collected on November 11, 2005........................................................17 Table 5. Percent Survival of Hyalella azteca in TIE Tests performed on the Chollas Creek Stormwater Sample Collected on January 2, 2006 .............................................................19 Table 6. Percent Survival of Hyalella azteca in TIE Tests performed on the Chollas Creek Stormwater Sample Collected on February 19, 2006..........................................................22 Table 7. Chemical Analyses of Chollas Creek Stormwater Samples Collected During the 2005- 2006 Monitoring Season......................................................................................................26 Weston Solutions, Inc. ii
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