Genetic approaches to development - Society for Developmental ...

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Forward Genetics in Drosophila any mutagen -Zygotic screen (e.g. Wieschaus & Nüsslein-Volhard) -Maternal-effect screen (e.g. Schüpbach & Wieschaus) -Maternal-effect clonal screen (e.g. Perrimon, St Johnston) -Adult clonal screen (e.g. Dickson) -Modifier (Enhancer/Suppressor) screen (e.g. Rubin)
Assays: You can only find what you are looking for Primary and secondary screens •Lethality •Sterility •Behavior •Pattern defects: segmentation, eye •Gene expression: RNA, protein, lacZ, GFP
Page 1 and 2: Genetic approaches to development:
Page 3 and 4: LIFE CYCLE STATISTICS C. elegans Dr
Page 6 and 7: A brief history of fly genetics •
Page 8 and 9: Why flies???? Genetics
Page 12 and 13: P General mutagenesis approach to i
Page 14 and 15: Drosophila germ cells from in germ
Page 16 and 17: The “No Germ Cells” Class wild-
Page 18 and 19: Flp/FRT technique * FRT > > * * > >
Page 20 and 21: OvoD technique Soma Germ line
Page 22 and 23: Enhancer/suppressor screens Sensiti
Page 24 and 25: Forward Genetics in Drosophila any
Page 26 and 27: Mis/overexpression screens, the goo
Page 28 and 29: ..but RNA of close homolog is local
Page 30 and 31: Mis/overexpression screens can iden
Page 32 and 33: Reverse Genetics in Drosophila -Dom
Page 34 and 35: Venken & Bellen, March 2005
Page 36 and 37: Germ Cells •Set aside early in de
Page 38 and 39: Germ cells are specificied by mater
Page 40 and 41: Fly and mouse germ cells: repressio
Page 42 and 43: pgc RNA is localized to germ plasm
Page 44 and 45: PGC may repress germ cell transcrip
Page 46 and 47: Target specificity suggests that PG
Page 48 and 49: Up-regulation of torso represses ge
Page 50 and 51: Antagonism between pgc and torso se
Page 52 and 53: Repression of somatic differentiati
Page 54 and 55: The niche concept for stem cell mai
Page 56 and 57: Dpp, a BMP2/4 homologue, is an inst
Page 58 and 59: The number of germ cells increases
Page 60 and 61:
PGC differentiation is repressed du
Page 62:
Primordial germ cell = germ line st
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