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Genetic approaches to development - Society for Developmental ...

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Different Types of Mutageneses<br />

Mutagenic<br />

Advantages<br />

Disadvantages<br />

Effect<br />

EMS<br />

(and other<br />

chemicals)<br />

Base pair<br />

changes (point<br />

mutations)<br />

Random<br />

Saturation<br />

Different types of mutations<br />

Slow gene identification<br />

Large-scale<br />

P-elements<br />

(and other<br />

transposons)<br />

DNA Insertions<br />

(mostly<br />

hypomorphic)<br />

Fast gene identification<br />

Flexible scale<br />

No saturation<br />

Non-random (hotspots)<br />

Deficiency kit<br />

Chromosome<br />

Small-scale<br />

Slow gene identification<br />

(and other<br />

aberrations)<br />

rearrangements<br />

(gene deletions)<br />

Fast screening<br />

Defined set<br />

No real saturation<br />

Ionizing<br />

radiations (Xrays,<br />

g-rays)<br />

Chromosome<br />

rearrangements<br />

(gene deletions)<br />

Random<br />

Gene deletions<br />

Slow gene identification<br />

No real saturation<br />

Inefficient

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