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Forward <strong>Genetic</strong>s in Drosophila any mutagen -Zygotic screen (e.g. Wieschaus & Nüsslein-Volhard) -Maternal-effect screen (e.g. Schüpbach & Wieschaus) -Maternal-effect clonal screen (e.g. Perrimon, St Johns<strong>to</strong>n) -Adult clonal screen (e.g. Dickson) -Modifier (Enhancer/Suppressor) screen (e.g. Rubin) P-element based -Enhancer-trap screen (e.g. Bellen, Jan) -Overexpression-trap screen (e.g. Rorth) -Protein-trap screen (e.g. Chia, Cooley)
Venken & Bellen, March 2005
Page 1 and 2: Genetic approaches to development:
Page 3 and 4: LIFE CYCLE STATISTICS C. elegans Dr
Page 6 and 7: A brief history of fly genetics •
Page 8 and 9: Why flies???? Genetics
Page 10 and 11: Forward Genetics in Drosophila any
Page 12 and 13: P General mutagenesis approach to i
Page 14 and 15: Drosophila germ cells from in germ
Page 16 and 17: The “No Germ Cells” Class wild-
Page 18 and 19: Flp/FRT technique * FRT > > * * > >
Page 20 and 21: OvoD technique Soma Germ line
Page 22 and 23: Enhancer/suppressor screens Sensiti
Page 26 and 27: Mis/overexpression screens, the goo
Page 28 and 29: ..but RNA of close homolog is local
Page 30 and 31: Mis/overexpression screens can iden
Page 32 and 33: Reverse Genetics in Drosophila -Dom
Page 34 and 35: Venken & Bellen, March 2005
Page 36 and 37: Germ Cells •Set aside early in de
Page 38 and 39: Germ cells are specificied by mater
Page 40 and 41: Fly and mouse germ cells: repressio
Page 42 and 43: pgc RNA is localized to germ plasm
Page 44 and 45: PGC may repress germ cell transcrip
Page 46 and 47: Target specificity suggests that PG
Page 48 and 49: Up-regulation of torso represses ge
Page 50 and 51: Antagonism between pgc and torso se
Page 52 and 53: Repression of somatic differentiati
Page 54 and 55: The niche concept for stem cell mai
Page 56 and 57: Dpp, a BMP2/4 homologue, is an inst
Page 58 and 59: The number of germ cells increases
Page 60 and 61: PGC differentiation is repressed du
Page 62: Primordial germ cell = germ line st

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