Genetic approaches to development - Society for Developmental ...

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Enhancer/suppressor screens Sensitized condition: sev ts + a ts mutation in kinase domain @ 22.7 o C R7 present @ 24.3 o C R7 absent EMS P sev - sev - ; + ; Bal, P(sev ts ) sev D2 + D X ; ; Y + + + F1 or sev - ; * ; * sev - /Y + Bal, P(sev ts ) Screen for absence of R7 at 22.7 o C
Different Mapping Methods in Drosophila Method tools principle result resolution pro con Classical meiotic mapping Deficiency mapping P-mediated male recombination mapping SNP mapping Based on visible markers Based on available deficiencies Based on available P insertions Based on molecular markers Meiotic Homologous recombination Complementation Meiotic Genetic recombination map position Chromosomal interval Position of mutation relative to P (proximal/distal) Molecular map position Non-molecular (not all markers cloned) Non-molecular (not all breakpoints molecularly mapped) Molecular (if position of P known) Molecular genetic location Fast mapping to a certain region Precise molecular interval Markers neutral Precise molecular interval Few visible markers available, often spaced far away from mutant Not all regions of the genome covered Interactions with other genes in Df Stepwise process, slow Still requires visible markers Expensive dependent on detection method
Page 1 and 2: Genetic approaches to development:
Page 3 and 4: LIFE CYCLE STATISTICS C. elegans Dr
Page 6 and 7: A brief history of fly genetics •
Page 8 and 9: Why flies???? Genetics
Page 10 and 11: Forward Genetics in Drosophila any
Page 12 and 13: P General mutagenesis approach to i
Page 14 and 15: Drosophila germ cells from in germ
Page 16 and 17: The “No Germ Cells” Class wild-
Page 18 and 19: Flp/FRT technique * FRT > > * * > >
Page 20 and 21: OvoD technique Soma Germ line
Page 24 and 25: Forward Genetics in Drosophila any
Page 26 and 27: Mis/overexpression screens, the goo
Page 28 and 29: ..but RNA of close homolog is local
Page 30 and 31: Mis/overexpression screens can iden
Page 32 and 33: Reverse Genetics in Drosophila -Dom
Page 34 and 35: Venken & Bellen, March 2005
Page 36 and 37: Germ Cells •Set aside early in de
Page 38 and 39: Germ cells are specificied by mater
Page 40 and 41: Fly and mouse germ cells: repressio
Page 42 and 43: pgc RNA is localized to germ plasm
Page 44 and 45: PGC may repress germ cell transcrip
Page 46 and 47: Target specificity suggests that PG
Page 48 and 49: Up-regulation of torso represses ge
Page 50 and 51: Antagonism between pgc and torso se
Page 52 and 53: Repression of somatic differentiati
Page 54 and 55: The niche concept for stem cell mai
Page 56 and 57: Dpp, a BMP2/4 homologue, is an inst
Page 58 and 59: The number of germ cells increases
Page 60 and 61: PGC differentiation is repressed du
Page 62: Primordial germ cell = germ line st

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