infertility treatments, genes and chromosomes

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DNA amplification: the polymerase chain reaction • Enzymatic method for copying specific DNA sequences C T T A C C G T G G T A A A T C G G A A T G G C A C C A T T T A G C • Essential for the analysis of <strong>genes</strong> in single cells See Wells & Sherlock 1998 for review
Generalized Diagnostic Methodology Biopsy PCR Mutation detection Polymorphism analysis Diagnosis Linked polymorphisms DNA fingerprint Report Contamination detection
Page 1 and 2: In vitro fertilisation and genetic
Page 3 and 4: Indications for IVF/ICSI Unexplaine
Page 5 and 6: Egg collection procedure
Page 7 and 8: Fertilization Mixing of sperm and e
Page 9 and 10: Embryo development 2‐cell embryo
Page 11 and 12: Genetic analysis of embryos
Page 13 and 14: Aneuploidy and IVF failure Chromoso
Page 15 and 16: Preimplantation genetic screening (
Page 17 and 18: Increase in implantation/pregnancy
Page 19 and 20: Problems with negative PGS studies
Page 21 and 22: Microarray comparative genomic hybr
Page 23 and 24: 46,XX‐10 +16 Microarray‐CGH (ar
Page 25 and 26: Blastocyst CGH‐ clinical results
Page 27 and 28: PGD of single gene disorders Altern
Page 29 and 30: PGD of single gene disorders Diseas
Page 31: Cleavage stage biopsy •Used by ma

infertility treatments, genes and chromosomes

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