haematology users' handbook - University College London Hospitals

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Routine Coagulation (page 1 of 2) Autolab 1 st Floor, Whitfield St Laboratories, 60 Whitfield St <strong>London</strong> W1T 4EU Tel: 0203 447 8547 or x 78547 • Citrated samples must be adequately filled (a 9:1 blood:citrate sample ratio is required for all citrated samples). Tests cannot be performed on underfilled, overfilled, haemolysed or clotted samples. • Paediatric citrated tubes are issued from the Haemostasis Laboratory to the Neonatal Unit, Paediatric A&E and Phlebotomy Outpatient departments and can be issued to other locations on request: contact x78549/78545 during routine hours. • Include clinical details on the request form, e.g. “patient receiving Warfarin/Heparin therapy” or “post op”; to facilitate appropriate testing and interpretation of results. • Send the request form and sample to the laboratory as rapidly as possible; notify the laboratory regarding very urgent requests on ext 78547 (during routine working hours) or Bleep 7060 (out of hours). • Grossly lipaemic samples may require manual testing, potentially delaying results. • Abnormal results that require further laboratory investigation and/or manual testing/confirmation may be delayed. Test Coagulation screen PT/INR, APTT, TT, Fibrinogen Specimen type 4.5ml Sodium Citrate Key Factors affecting tests Heparin & Warfarin. Delayed receipt of sample. May not be possible on grossly lipaemic samples. Note Reference Range Out of hours service Heparin & warfarin therapy affects results and determines appropriate testing please indicate that the patient is on heparin and/or warfarin on the request form. PT: 10-12 secs APTT: 25-37 secs TT: 15.5-18.5 secs Fib: 1.5 – 4.0 g/L Service for clinically urgent requests Time Limit for requesting additional tests from time of venesection 5 hours Urgent: 1hr Turnaround time from receipt of sample Nonurgent: 4 hrs HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 18 of 38 Authors: Karen Orfinada (Senior Biomedical Scientist) Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
Routine Coagulation (page 2 of 2) D-dimers Test (Unfractionated) Heparin Monitoring: APTT ratio Specimen type 4.5ml Sodium Citrate 4.5ml Sodium Citrate Key Factors affecting tests Delayed receipt of sample. Note Out of hours service This test can be added for an existing citrated sample. Please indicate Heparin therapy on the request form (tick box or handwrite). Normal cut off: 0 – 0.55 mg/L FEU DVT/PE cut off:
Page 1 and 2: HAEMATOLOGY AND BLOOD TRANSFUSION U
Page 3 and 4: General Information Location The Ha
Page 5 and 6: Medical Staff Consultants Dr Marie
Page 7 and 8: Vacutainer Guide Becton Dickinson V
Page 9 and 10: Sample Transport to the Laboratory
Page 11 and 12: Routine Haematology (page 2 of 3) T
Page 13 and 14: Blood Transfusion (page 1 of 5) 1 s
Page 15 and 16: Blood Transfusion (page 3 of 5) Tes
Page 17: Blood Transfusion (page 5 of 5) Gui
Page 21 and 22: Haemostasis (page 2 of 6) Test Spec
Page 23 and 24: Test Specimen type Key Factors affe
Page 25 and 26: Haemostasis (page 6 of 6) Test Spec
Page 27 and 28: Special Haematology (page 2 of 3) T
Page 29 and 30: Flow Cytometry (HIV and Malignancy
Page 31 and 32: Flow Cytometry (HIV and Cell Marker
Page 33 and 34: Flow Cytometry (HIV and Cell Marker
Page 35 and 36: Leukaemia Cytogenetics (page 2 of 5
Page 37 and 38: Leukaemia Cytogenetics (page 4 of 5

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