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haematology users' handbook - University College London Hospitals

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Test Specimen type Key Factors<br />

affecting<br />

tests<br />

Notes<br />

Reference<br />

Range<br />

Out of hours<br />

service<br />

Time Limit for<br />

requesting<br />

additional<br />

tests from<br />

time of<br />

venesection<br />

Turnaround<br />

time from<br />

receipt of<br />

sample<br />

Factor Assays<br />

FVIII, FIX, FXI, FXII<br />

FII, FV, FVII, FX<br />

FXIII<br />

Lupus Anticoagulant<br />

test<br />

Dilute Russell’s Viper<br />

Venom Time test<br />

or Taipan Venom<br />

Time test (on<br />

warfarin).<br />

4.5ml Sodium<br />

Citrated samples –<br />

contact the<br />

laboratory for the<br />

number of samples<br />

required (dependent<br />

on tests requested).<br />

3 x 4.5ml Sodium<br />

Citrated samples<br />

Heparin and<br />

Warfarin.<br />

Acute illness<br />

(contact the<br />

laboratory).<br />

Delayed<br />

receipt of<br />

sample<br />

Warfarin,<br />

Heparin and<br />

Rivaroxaban<br />

therapy.<br />

Delayed<br />

receipt of<br />

sample<br />

Required factor assays<br />

must be specified on the<br />

request form.<br />

Lupus Anticoagulant testing<br />

is inappropriate when<br />

patients are receiving<br />

heparin therapy.<br />

An SST sample is required<br />

if Cardiolipin Antibody<br />

testing is also requested.<br />

Contact the<br />

laboratory<br />

Factor VIII<br />

and Factor IX<br />

only: by<br />

agreement<br />

with the<br />

Haematology<br />

Consultant<br />

on call and<br />

subject to<br />

staff<br />

availability.<br />

No out of<br />

hours service<br />

Contact the<br />

Laboratory<br />

Contact the<br />

laboratory<br />

3 working<br />

days<br />

3 working<br />

days<br />

Heparin induced<br />

thrombocytopaenia<br />

(HIT) screen<br />

Heparin:PF4<br />

antibody assay<br />

1 x 4.5ml Sodium<br />

Citrated sample<br />

(preferred)<br />

Urgent test:<br />

send the<br />

sample to the<br />

lab.<br />

Immediately.<br />

This test MUST be<br />

authorised by a<br />

Haematology Consultant<br />

(i.e. not a SpR): contact via<br />

the Haematology SpR<br />

(bleep 7044/7000) or<br />

switchboard out of hours.<br />

Contact the<br />

laboratory<br />

By agreement<br />

with the<br />

Haematology<br />

Consultant<br />

on call and<br />

subject to<br />

staff<br />

availability.<br />

Contact the<br />

Laboratory<br />

Urgent:<br />

1 working<br />

day<br />

Non-urgent:<br />

5 working<br />

days<br />

HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 24 of 38<br />

Authors: Karen Orfinada (Senior Biomedical Scientist)<br />

Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015

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