haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Flow Cytometry (HIV and Malignancy Cell Markers) (page 1 of 5)<br />
2 nd Floor, 60 Whitfield St, <strong>London</strong> W1T 4EU Hours: 09.00 – 17.15 Monday - Friday Tel: 0203 447 9603 or x 79603<br />
• Please see report or telephone laboratory for reference ranges<br />
Test Specimen Type Key<br />
Factors<br />
affecting<br />
tests<br />
Notes Out of hours service Time Limit for<br />
requesting<br />
additional<br />
tests from<br />
time of<br />
venesection<br />
Turnaround<br />
time from<br />
receipt of<br />
sample (in<br />
working days)<br />
Acute Leukaemia<br />
screen. This includes<br />
Myeloperoxidase stain,<br />
Esterase stain and Tdt.<br />
Bone Marrow in EDTA<br />
(can be performed on<br />
peripheral blood if<br />
bone marrow is not<br />
available)<br />
No minimum volume.<br />
Analysis can also be<br />
carried out on CSF,<br />
pleural and ascites<br />
fluid.<br />
Sample not<br />
kept at<br />
room<br />
temperature<br />
Pleural,<br />
Ascites and<br />
CSF<br />
samples not<br />
being spun<br />
onto slides<br />
within 12<br />
hours of<br />
being taken.<br />
Please discuss with<br />
Haematology SpR (Bleep<br />
7000/7001) before sending.<br />
Please send samples<br />
directly to Flow Cytometry<br />
laboratory.<br />
Please let flow cytometry<br />
laboratory know to expect<br />
sample.<br />
Transport samples at room<br />
temperature.<br />
The service at weekends<br />
and Bank Holidays is for<br />
new acute leukaemia<br />
screens only and must be<br />
backed by Consultant<br />
Haematologist. Contact<br />
Laboratory on-call bleep<br />
7060. Clinical staff must<br />
be available for<br />
interpretation of results as<br />
this will not be provided by<br />
laboratory staff.<br />
Within 24<br />
hours<br />
Urgent:<br />
Results can be<br />
sent out within<br />
a 8 hours after<br />
being received<br />
in laboratory<br />
Non-urgent:<br />
48hrs.<br />
Chronic Leukaemia<br />
screen<br />
Peripheral Blood in<br />
EDTA<br />
Can be performed on<br />
BM, CSF, pleural fluid<br />
and Ascites fluid.<br />
No minimum volume.<br />
Sample not<br />
kept at<br />
room<br />
temperature<br />
Please send samples<br />
directly to flow cytometry<br />
lab.<br />
Please let flow cytometry<br />
laboratory know to expect<br />
the samples.<br />
Transport samples at room<br />
temperature.<br />
No service provided Within 24<br />
hours<br />
Urgent:<br />
Results can be<br />
sent out within<br />
a few hours<br />
after being<br />
received in<br />
laboratory<br />
Non-urgent:<br />
48hrs.<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 29 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
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