haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Special Haematology (page 2 of 3)<br />
Test Specimen type Key Factors<br />
affecting tests<br />
Notes<br />
Out of<br />
hours<br />
service<br />
Time Limit for<br />
requesting<br />
additional tests<br />
from time of<br />
venesection<br />
Turnaround<br />
time from<br />
receipt of<br />
sample (in<br />
working days)<br />
Haemoglobin H stain<br />
4.5ml EDTA or Lithium<br />
heparin<br />
Age of sample<br />
Samples greater than 48 hours<br />
old are unsuitable for this test.<br />
No service<br />
provided<br />
24 hours 24 – 48 hours<br />
Heinz Body<br />
Preparation<br />
4.5ml EDTA or Lithium<br />
heparin<br />
Age of sample<br />
Samples greater than 48 hours<br />
old are unsuitable for this test.<br />
No service<br />
provided<br />
48 hours 72 hours<br />
Hereditary<br />
Spherocytosis<br />
Dye Binding Assay<br />
4.5ml EDTA Age of sample Please arrange with laboratory<br />
Samples greater than 24 hours<br />
old are unsuitable for this test.<br />
No service<br />
provided<br />
Within 8 hours<br />
72 hours<br />
Red Cell Osmotic<br />
Fragility<br />
Red Cell Oxygen<br />
Dissociation<br />
Globin Chain<br />
Biosynthesis<br />
4.5ml Lithium heparin Please arrange with laboratory No service<br />
provided<br />
4.5ml Lithium heparin Please arrange with laboratory No service<br />
provided<br />
3 x 4.5ml Lithium<br />
Please arrange with laboratory No service<br />
heparin (10ml blood<br />
provided<br />
required)<br />
Within 8 hours<br />
Within 8 hours<br />
Within 8 hours<br />
72 hours<br />
48 hours<br />
Urgent<br />
5 working days<br />
Non urgent<br />
10 working<br />
days<br />
Haemoglobinopathy<br />
DNA and<br />
Haemochromatosis<br />
Erythropoietin<br />
2 - 4 x 4.5ml EDTA<br />
(10 - 20ml blood<br />
required)<br />
7ml clotted sample (no<br />
anticoagulant)<br />
Referred to Haemoglobinopathy<br />
Genetics, UCLH<br />
Via Haematology consultant.<br />
Referred to Kings <strong>College</strong><br />
Hospital<br />
No service<br />
provided<br />
No service<br />
provided<br />
Additional<br />
samples are<br />
required for test.<br />
Additional<br />
samples are<br />
required for test.<br />
2-20 working<br />
days<br />
10 -15 working<br />
days<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 27 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015