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haematology users' handbook - University College London Hospitals

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Special Haematology (page 2 of 3)<br />

Test Specimen type Key Factors<br />

affecting tests<br />

Notes<br />

Out of<br />

hours<br />

service<br />

Time Limit for<br />

requesting<br />

additional tests<br />

from time of<br />

venesection<br />

Turnaround<br />

time from<br />

receipt of<br />

sample (in<br />

working days)<br />

Haemoglobin H stain<br />

4.5ml EDTA or Lithium<br />

heparin<br />

Age of sample<br />

Samples greater than 48 hours<br />

old are unsuitable for this test.<br />

No service<br />

provided<br />

24 hours 24 – 48 hours<br />

Heinz Body<br />

Preparation<br />

4.5ml EDTA or Lithium<br />

heparin<br />

Age of sample<br />

Samples greater than 48 hours<br />

old are unsuitable for this test.<br />

No service<br />

provided<br />

48 hours 72 hours<br />

Hereditary<br />

Spherocytosis<br />

Dye Binding Assay<br />

4.5ml EDTA Age of sample Please arrange with laboratory<br />

Samples greater than 24 hours<br />

old are unsuitable for this test.<br />

No service<br />

provided<br />

Within 8 hours<br />

72 hours<br />

Red Cell Osmotic<br />

Fragility<br />

Red Cell Oxygen<br />

Dissociation<br />

Globin Chain<br />

Biosynthesis<br />

4.5ml Lithium heparin Please arrange with laboratory No service<br />

provided<br />

4.5ml Lithium heparin Please arrange with laboratory No service<br />

provided<br />

3 x 4.5ml Lithium<br />

Please arrange with laboratory No service<br />

heparin (10ml blood<br />

provided<br />

required)<br />

Within 8 hours<br />

Within 8 hours<br />

Within 8 hours<br />

72 hours<br />

48 hours<br />

Urgent<br />

5 working days<br />

Non urgent<br />

10 working<br />

days<br />

Haemoglobinopathy<br />

DNA and<br />

Haemochromatosis<br />

Erythropoietin<br />

2 - 4 x 4.5ml EDTA<br />

(10 - 20ml blood<br />

required)<br />

7ml clotted sample (no<br />

anticoagulant)<br />

Referred to Haemoglobinopathy<br />

Genetics, UCLH<br />

Via Haematology consultant.<br />

Referred to Kings <strong>College</strong><br />

Hospital<br />

No service<br />

provided<br />

No service<br />

provided<br />

Additional<br />

samples are<br />

required for test.<br />

Additional<br />

samples are<br />

required for test.<br />

2-20 working<br />

days<br />

10 -15 working<br />

days<br />

HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 27 of 38<br />

Authors: Karen Orfinada (Senior Biomedical Scientist)<br />

Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015

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