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haematology users' handbook - University College London Hospitals

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Flow Cytometry (HIV and Cell Markers) (page 2 of 5)<br />

Test Specimen Type Key<br />

Factors<br />

affecting<br />

tests<br />

Notes<br />

Out of hours<br />

service<br />

Time Limit<br />

for<br />

requesting<br />

additional<br />

tests from<br />

time of<br />

venesection<br />

Turnaround<br />

time from<br />

receipt of<br />

sample (in<br />

working days)<br />

Lymphoma screen<br />

Bone Marrow or<br />

Peripheral Blood<br />

(Can be performed on<br />

existing FBC sample)<br />

No minimum volume.<br />

Sample not<br />

kept at<br />

room<br />

temperature<br />

Please send samples directly to<br />

flow cytometry lab.<br />

Please let flow cytometry<br />

laboratory know to expect<br />

sample.<br />

Transport samples at room<br />

temperature.<br />

No service provided Within 24<br />

hours<br />

Urgent: Results<br />

can be sent out<br />

within a few<br />

hours after being<br />

received in<br />

laboratory<br />

Non-urgent:<br />

48hrs.<br />

Bone Marrow staining<br />

8 x bone marrow<br />

smears<br />

If BM in<br />

EDTA has<br />

been<br />

received<br />

instead of<br />

slides<br />

The laboratory registrar / SHO<br />

should be contacted for bone<br />

marrow requests. Slides must be<br />

labelled in pencil with at least 3<br />

points of patient id and the date of<br />

sampling.<br />

BM received out of<br />

hours are not<br />

stained. If BM are<br />

urgent and need to<br />

be looked at out of<br />

hours, please bleep<br />

on-call laboratory<br />

staff to let them<br />

know to stain slides.<br />

Within 24<br />

hours<br />

Urgent ( i.e.<br />

Acute<br />

Leukaemia):<br />

1hr for staining +<br />

same day for<br />

reporting by<br />

Haem<br />

SpR/consultant<br />

Non-urgent:<br />

24hrs for staining<br />

+ further 4<br />

working days for<br />

reporting by<br />

Haem<br />

consultant/<br />

SpR<br />

HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 30 of 38<br />

Authors: Karen Orfinada (Senior Biomedical Scientist)<br />

Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015

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