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Chapter 5 Indoor Propagation of Medicinal Cannabis..........................................102 5.1 INTRODUCTION........................................................................................102 5.2 AIM AND OBJECTIVES .............................................................................106 5.3 MATERIALS ...............................................................................................107 5.3.1 Plant Propagation and Drying Materials...............................................107 5.3.2 Germplasm Details ..............................................................................108 5.3.3 Light Measurement and Weighing Equipment .....................................108 5.3.4 Growth Medium ...................................................................................108 5.4 METHODS .................................................................................................109 5.4.1 Routine Propagation and Plant Production Methods ...........................109 5.4.1.1 Seed sowing and transplantation of seedlings ..............................109 5.4.1.2 Production of Cuttings (Clones).....................................................109 5.4.1.3 Nurturing Vegetative Growth of Seedlings and Cuttings. ..............110 5.4.1.4 Induction and Maintenance of Flowering.......................................110 5.4.1.5 Biological Pest Control ..................................................................110 5.4.1.6 Harvesting .....................................................................................110 5.4.1.7 Crop Drying ...................................................................................111 5.4.1.8 Stripping ........................................................................................112 5.4.1.9 Garbling.........................................................................................112 5.4.1.10 Storage........................................................................................112 5.4.1.11 Environmental Control System....................................................113 5.4.2 Specific Methods .................................................................................113 5.4.2.1 Uniformity of Plants Grown from Cuttings or Seed........................113 5.4.2.2 Effect of Duration of Flowering Period on Yield ..........................114 5.4.2.3 Effect of Daylength on Cannabinoid Profile (Part 1) Comparison of 12 and 13 hour daylength ..................................................................114 5.4.2.4 Effect of Daylength on Cannabinoid Profile (Part 2) Comparison of 11 and 12 hour daylength...............................................................115 5.4.2.5 Plant height assessment ...............................................................115 5.4.2.6 Stigma senescence assessment...................................................116 ix
5.4.2.7 Plant Weight Assessment .............................................................116 5.4.2.8 Cannabinoid Content and Profile...................................................116 5.4.2.9 Effect of Irradiance Level on Plant and Cannabinoid Yield............117 5.4.2.10 Effect of the length of flowering period on the cannabinoid profile of heterozygous plants of the mixed THC/CBD chemotype. ..............118 5.4.2.11 Statistical Analysis.......................................................................118 5.5 RESULTS AND DISCUSSION ...................................................................119 5.5.1 Comparison of the Yield and Uniformity of Plants Grown from Cuttings or Seeds...............................................................................119 5.5.2 Effect of Irradiance Level on Plant and Cannabinoid Yield ..................120 5.5.3 Effect of Duration of Flowering Period on BRM and Cannabinoid Yield ...................................................................................................126 5.5.3.1 Effect of Duration of Harvest Period on Ratio of THC and CBG in THC Chemovars ................................................................................127 5.5.3.2 Effect of the length of flowering period on profile of heterozygous chemotypes with mixed THC/CBD profiles ........................................129 5.5.4 Effect of Daylength on Plant Development and Cannabinoid Profile ...130 5.5.4.1 Comparison of Twelve and Thirteen Hour Daylength Regimes.....130 5.5.4.2 Comparison of Eleven and Twelve Hour Daylength Regimes.......136 5.5.4.3 Review of the comparisons of plants induced to flower on daylengths 11, 12 and 13 hours.........................................................140 5.6 CONCLUSIONS .........................................................................................141 Chapter 6 The Outdoor Propagation of Phytopharmaceutical Cannabis .............143 6.1 INTRODUCTION........................................................................................143 6.2 AIM and OBJECTIVES...............................................................................144 6.2.1 The effect of growing environment on female plant development........145 6.2.2 Comparison of the secondary metabolite yield and profile of fresh plant material and enriched trichome preparations made from them ...145 x
Page 1 and 2: THE PROPAGATION, CHARACTERISATION A
Page 3 and 4: Cannabis sativa L cv Gayle CBD Chem
Page 5 and 6: List of Publications Potter, D.J.,
Page 7 and 8: CHAPTER 2 CHARACTERISATION OF ILLIC
Page 9: 3.5.3 Effect of capitate stalked tr
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Page 15 and 16: Figure 2.4. (a) A herb grinder in c
Page 17 and 18: Figure 3.11. (a) Secretory cells st
Page 19 and 20: Figure 3.23. The mean CBN content (
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Page 23 and 24: List of Tables Table 1.1. Examples
Page 25 and 26: Table 4.9. The cannabinoid profile
Page 27 and 28: Table 6.6. A comparison of the terp
Page 29 and 30: CBDA Cannabidiolic acid CBG Cannabi
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Chapter 2 Characterisation of Illic
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Chapter 4. The Function and Exploit
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Chapter 5. Indoor Propagation of Me
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Chapter 6. The Outdoor Propagation
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Chapter 7 General Discussion Chapte
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Chapter 7 General Discussion be at
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Chapter 7 General Discussion to be
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Chapter 7 General Discussion photos
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References Baker, R., 2002. Commerc
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References Cerniak, L., 1985. The G
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References Dayanandan, P. & Kaufman
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References Fairbairn, J.W., 1972. T
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References Ghosal, S., Singh, S., B
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References Henderson, S.M., 1973. E
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References Johnson, J.R. and Potts,
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References Mahlberg, P.G., Hammond,
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References de Meijer, E.D.M., 1994.
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References Page, C.P., Hoffman, B.,
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References Raman, A.R., 1998. The C
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References Russo, E. B., Jiang, H.
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References Struik, P.C., Amaducci,
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References UNODC. United Nations Of
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References Wilkinson, T. J., 2006.
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Appendix 8.1 Analysis of cannabinoi
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Appendix 8.1 Analysis of cannabinoi
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Appendix 8.2 Analysis of terpene co
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Appendix 8.3 A Comparison of the St
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Appendix 8.5 Definitions of Social
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