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stained with Fast Blue. The still-wet sample was temporarily dry-mounted and viewed
using incident light. 59
Figure 3.5. (a) a row of antherial sessile trichomes showing their normal distribution in
the furrow of a cannabis anther. These anthers were captured in incident light through a
low-power microscope. (b) closer view of antherial trichomes. 61
Figure 3.6. A capitate stalked trichome (centre) between two cystolythic trichomes. The
specimen is temporarily dry-mounted and illuminated from below. The secretory cells
are out-of-focus due to the optical distortion within the glandular head. 62
Figure 3.7. Two dry-mounted capitate stalked trichomes viewed in transmitted light.
Most of the features are out-of-focus. In the right-hand trichome, a crisp view of cells
within the secretory cell disk appears as an in-focus image. However these appear to
be located outside of the trichome structure, due to the refractive properties of the resin
head. 63
Figure 3.8. (a) A temporarily dry-mounted capitate stalked trichome viewed in
transmitted light. An irregular arrangement of poorly-defined secretory cells is visible at
the base of the glandular head; (b) A capitate stalked trichome, temporarily mounted in
glycerol and viewed in transmitted light, and (c) an illustration of a capitate stalked
trichome on Cannabis sativa by Briosi and Tognini (1894). 64
Figure 3.9. (a and b). Similar sized capitate stalked trichomes temporarily mounted
under hemp oil. The samples are viewed in transmitted light. Possibly because of a
similarity in the refractive index of the oil and the secretory cell contents, these cells
appear clear. The outer membrane at the base of the glandular head appears dark and
opaque. 65
Figure 3.10. A contrasting pair of resin heads on capitate stalked glandular trichomes,
naturally orientated to allow sideways-on (left) and overhead views (right). The
specimen is temporarily mounted in a 70% v/v aqueous solution of glycerol and
illuminated from below. 66
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