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Table 5.10 A comparison of the proportion of senesced stigmas on ten clones in twelve and eleven hour daylength regimes when assessed eight and ten weeks after the induction of flowering. Just one overall visual assessment was made for each clone. 137 Table 5.11 The effect of day length during flowering on cannabinoid profile. Results shown are the proportion of CBG expressed as a % of the CBG+THC+CBD total, in six clones after eight weeks in short daylength. 140 Table 6.1. Propagation Materials and Equipment used in the field trials program to evaluate the outdoor propagation of Cannabis sativa L. 146 Table 6.2. Summary of Agronomic and Yield Data from field trials performed between 2000 and 2006. *The 2000 trial was performed before commencement of this thesis, and the data is included for comparison. 150 Table 6.3. A comparison of the pattern of inflorescence development and stigma senescence in indoor and outdoor crops of cannabis chemovar G5. The stages of development of the glasshouse crop are shown from the point at which the plants are moved into a 12hour light/12hour dark until they are routinely harvested eight weeks later. The field crop development is shown from mid-August, just before stigma formation commenced. 153 Table 6.4. A comparison of the terpene profile of freshly harvested fully mature field grown cannabis leaf and flower material (cultivar G5 M16) and ETP made from the same fresh material (2005 Field Trial). Also included is ETP made from similar mature glasshouse grown material. The data show the relative peak areas when assessed by GC at Botanix Ltd. In each case one batch of material was analysed. 155 Table 6.5. The changing proportions of CBG and THC with respect to CBD (Mean ± sd) in enriched trichome preparations produced from plants harvested at weekly intervals between 17 th September and 15 th October. Just one ETP sample was made on each date after bulking together one plant from each of the seven replicates. Three subsamples of each preparation were analysed. 160 xxv
Table 6.6. A comparison of the terpene profile of steam-distillates of enriched trichome preparations made from freshly harvested plants on five dates between 17 th September and 15 th October 2006. One bulked sample was analysed on each date. 163 Table 6.7. Ratio of eight terpenes in steam distilled enriched trichome preparations made from freshly harvested field grown plants of cultivar G5 M16. The result for each terpene is expressed as a weight percentage (% w/w) of the total within each column. The table also shows the ratio of myrcene (the dominant monoterpene) and trans-caryophylene (the dominant sesquiterpene). 164 Table 6.8. Comparison of myrcene/trans-caryophylene ratios when calculated from Relative Peak Areavalues and w/w data. 165 Table 6.9. The relative proportions of THC and CBD synthesised in heterozygous B T B D clones derived from variety G159. The proportion of THC produced is shown as a % of the THC+CBD total. Just one sample of dry inflorescence material was analysed from each plant. 165 Table 6.10. Summary of pest problems experienced, in decreasing order of magnitude using a subjective 1-5 score. * Botrytis initially minor but extremely severe in late harvested plots. † Symptoms not recognized as pest damage until 2005. ‡ Aphids absent in trial plots but moderate infestation of black bean aphid Aphis fabae observed on neighbouring cannabis seed-crop with lower secondary metabolite content. 168 Table 6.11. The level of infection with Botyrytis cinerea observed on plants harvested on five dates between 17 th September and 15 th October 2006. Scores are the mean % infection rates in the seven plots of each treatment. 169 xxvi
Page 1 and 2: THE PROPAGATION, CHARACTERISATION A
Page 3 and 4: Cannabis sativa L cv Gayle CBD Chem
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References Baker, R., 2002. Commerc
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References Cerniak, L., 1985. The G
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References Dayanandan, P. & Kaufman
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References Fairbairn, J.W., 1972. T
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References Ghosal, S., Singh, S., B
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References Henderson, S.M., 1973. E
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References Johnson, J.R. and Potts,
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References Mahlberg, P.G., Hammond,
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References de Meijer, E.D.M., 1994.
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References Page, C.P., Hoffman, B.,
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References Raman, A.R., 1998. The C
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References Russo, E. B., Jiang, H.
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References Struik, P.C., Amaducci,
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References UNODC. United Nations Of
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References Wilkinson, T. J., 2006.
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Appendix 8.1 Analysis of cannabinoi
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Appendix 8.1 Analysis of cannabinoi
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Appendix 8.2 Analysis of terpene co
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Appendix 8.3 A Comparison of the St
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Appendix 8.5 Definitions of Social
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