finalfullthesisdjpotter

6.2.3 The effects of harvest timing on secondary metabolite yield and profile
.............................................................................................................145
6.2.4 Comparison of the secondary metabolite profiles of glasshouse and
outdoor grown plants ...........................................................................145
6.2.5 Evaluation of outdoor pest and disease issues....................................145
6.2.6 Evaluation of Crop Drying Methods .....................................................145
6.3 MATERIALS ...............................................................................................146
6.4 GENERAL AGRONOMIC METHODS........................................................146
6.4.1 Seedbed Location, Preparation and Crop Establishment ....................146
6.4.2 Field Trial Design.................................................................................147
6.4.3 Soil Nutrition ........................................................................................147
6.4.4 Pest and Disease Monitoring and Management ..................................147
6.4.5 Harvest ................................................................................................147
6.4.6 Crop Drying and Stripping....................................................................147
6.4.7 Assessment of Crop Development ......................................................148
6.5 Secondary Metabolite Purification and Analytical Methods ........................148
6.5.1 Production and Collection of Enriched Trichome Preparations............148
6.5.2 Steam distillation of trichome rich preparations ...................................148
6.5.3 Steam distillation fresh foliar and floral material...................................149
6.6 Statistical methods .....................................................................................149
6.7 RESULTS and DISCUSSION.....................................................................149
6.7.1 Observations on Crop Establishment and Plant Development ............149
6.7.2 Comparison of the secondary metabolite yield and profile of fresh
plant materials and enriched trichome preparations made from them .154
6.7.3 The effects of harvest timing and growth stage on yield and
cannabinoid profile.............................................................................156
6.7.3.1 Botanical Raw Material Yield.........................................................156
6.7.3.2 Potency of CBD chemovars ..........................................................157
6.7.3.3 CBD Yield......................................................................................158
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