Cell death, transduction pathway and secretion tracked by flow ...

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II.Apoptosis analysis • DNA fragmentation : TUNEL Assay (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) • BrdU is added to DNA break by TdT, anti-BrdU-FITC/PI • dUTP-FITC directly Martin and al, Current Protocols in molecular biology ; 2001 www.phoenixflow.com www.Compucyte.com
II.Apoptosis analysis • Mitochondrial events : APO2.7 protein detection Membrane depolarisation JC-1/DiOC(6)/Mitotracker... Bax/Bad/Bcl-2/Bcl-xL detection Caspase global or specific activity S Fulda and K-M Debatin Oncogene, 2006 : 25, 4798-4811
Page 1 and 2: Cell death, transduction pathway an
Page 3 and 4: I.Critical point • Hydrodynamic f
Page 5 and 6: I.Optical Bench • Collects light
Page 7 and 8: I.Data representation « Dot Plot
Page 9 and 10: I.Data representation Images were m
Page 11 and 12: II.Basic death analysis • Membran
Page 13 and 14: II.Apoptosis analysis • Annexin V
Page 15: II.Apoptosis analysis • DNA fragm
Page 19 and 20: II.Apoptosis analysis • Bax/Bcl-2
Page 21 and 22: II.Apoptosis analysis • Active fo
Page 23 and 24: III.Transduction pathway analysis
Page 33 and 34: IV.Cytokine secretion analysis •
Page 35 and 36: IV.Cytokine secretion analysis ELIS
Page 37: V.Conclusion • Flow cytometry is

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