Cell death, transduction pathway and secretion tracked by flow ...

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III.Transduction pathway analysis • Evaluation of cancer cells sensitivity to chemotherapy • Appreciate cytokines effects • Titration of an agonist Desplat and al, Cytometry, 2004;62A:35-45 Krutzik and al, Cytometry, 2003;55A:61-70 Krutzik and al, Clin. Immunol, 2004;110:206-21
III.Transduction pathway analysis • Immunology : Following activation of cells after stimulation with cytokines, GF, tetramers, vaccination trial • Hematology/pharmacology : Following activation status of cancer cells, pathological cells (auto-immune disease), treatment effectiveness • Discovery of signaling pathways • Discovery of pathway blocking or activating molecules • Prognostic marker in disease • Limits : No localization informations unlike fluorescence microscopy Accessibility to the antigen depends of protocol Sensitivity is higher than WB, no enzyme amplification Quantity of data, need time to analyse Krutzik and al, Clin. Immunol, 2004;110:206-21
Page 1 and 2: Cell death, transduction pathway an
Page 3 and 4: I.Critical point • Hydrodynamic f
Page 5 and 6: I.Optical Bench • Collects light
Page 7 and 8: I.Data representation « Dot Plot
Page 9 and 10: I.Data representation Images were m
Page 11 and 12: II.Basic death analysis • Membran
Page 13 and 14: II.Apoptosis analysis • Annexin V
Page 15 and 16: II.Apoptosis analysis • DNA fragm
Page 17 and 18: II.Apoptosis analysis • Mitochond
Page 19 and 20: II.Apoptosis analysis • Bax/Bcl-2
Page 21 and 22: II.Apoptosis analysis • Active fo
Page 23 and 24: III.Transduction pathway analysis
Page 29: III.Transduction pathway analysis
Page 33 and 34: IV.Cytokine secretion analysis •
Page 35 and 36: IV.Cytokine secretion analysis ELIS
Page 37: V.Conclusion • Flow cytometry is

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