Cell death, transduction pathway and secretion tracked by flow ...

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II.Apoptosis analysis • Global or specific caspase activity : FLICA (FLuorescent Inhibitor of CAspase) Substrate reacts with all active caspases, covalently bound to caspase cystein VAD sequence specific, (DEVD 3,7; LETD 8) Reporter emits green light Non covalently bound molecules leave the cell Image from www.invitrogen.comc • Caspase 3 specific substrate : PhiPhiLux Arnoult D and al, Cell Death and Differentiation, 2003;10:1240-1252 www.PhiPhiLux.com
II.Apoptosis analysis • Active form of caspase 3 Image from Beckman Coulter http://home.ncifcrf.gov/ccr/flowcore/caspase3.htm
Page 1 and 2: Cell death, transduction pathway an
Page 3 and 4: I.Critical point • Hydrodynamic f
Page 5 and 6: I.Optical Bench • Collects light
Page 7 and 8: I.Data representation « Dot Plot
Page 9 and 10: I.Data representation Images were m
Page 11 and 12: II.Basic death analysis • Membran
Page 13 and 14: II.Apoptosis analysis • Annexin V
Page 15 and 16: II.Apoptosis analysis • DNA fragm
Page 17 and 18: II.Apoptosis analysis • Mitochond
Page 19: II.Apoptosis analysis • Bax/Bcl-2
Page 23 and 24: III.Transduction pathway analysis
Page 33 and 34: IV.Cytokine secretion analysis •
Page 35 and 36: IV.Cytokine secretion analysis ELIS
Page 37: V.Conclusion • Flow cytometry is

Cell death, transduction pathway and secretion tracked by flow ...

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