Bacteria from Fish and Other Aquatic Animals - Survival-training.info

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Aquatic Animal Species and Organism Relationship 5 Bream Black sea bream (Acanthopagrus latus Houttuyn). Known as Yellowfin seabream Common bream, Carp bream (Abramis brama Linnaeus) One-spot sea bream (Diplodus sargus kotschyi Steindachner) Red sea bream, Japanese seabream (Pagrus major Temminck and Schlegel). Family Sparidae Sea bream (Sparus auratus Linnaeus). Also known as Gilt-head sea bream. Family Sparidae Silver bream, White bream (Blicca bjoerkna Linnaeus) 1. Pseudomonas anguilliseptica 1. Haemorrhage and ulcerative lesions Not susceptible to infection with Aeromonas salmonicida ssp. achromogenes 1. Edwardsiella tarda 2. Listonella anguillarum 3. Tenacibaculum maritimum 1. Listonella anguillarum 2. Photobacterium damselae ssp. damselae 3. Photobacterium damselae ssp. piscicida 4. Pseudomonas anguilliseptica 5. Streptococcus agalactiae 6. Streptococcus iniae 7. Vibrio alginolyticus 8. Vibrio harveyi 9. Vibrio splendidus Aeromonas salmonicida ssp. achromogenes Large open lesions, areas of descalation. Typical ‘furuncles’ not seen 1. Septicaemia, focal suppurative or granulomatous lesions, cutaneous ulcerations 2. Lethargy, distended abdomen, haemorrhages on fins and tail, pale liver 3. No external changes except anus red and protruded, abdomen distended, fluid in abdominal cavity, congested spleen, petechial haemorrhages on liver, granulomatous lesions in spleen and kidney, multifocal tissue necrosis 4. Erratic swimming at water surface, sink to the bottom of the cage and die. Ascites, renal haemorrhage 5. Haemorrhagic areas on body, mouth, eye, operculum and fins 1, 7, 8, 9. Ulcers, haemorrhages, exophthalmia Large open skin lesions surrounded by areas of descalation. Typical ‘furuncles’ not seen Streptococcus iniae 1. Associated with ‘winter disease’. Septicaemia 225 569 848 Mortalities 534 Not susceptible to infection with Streptococcus iniae 1. Edwardsiellosis 2, 3. Infection 1, 2. Mortality 3. Fish pasteurellosis 4. Associated with ‘winter disease’, haemorrhagic septicaemia associated with keratitis 5. Streptococcosis – epizootic with 100% mortality 6. Exudative meningitis and panophthalmitis 1, 3, 7, 8. 9. Primary pathogens in virulence studies 848 57 58 60 96 225 242 751 786 853 Furunculosis, mortality 534 Carp Bighead carp (Aristichthys nobilis) Edwardsiella ictaluri 627 continued
6 Chapter 1 Table 1.1. Continued. Host Pathogen Normal flora Tissue site Disease status Ref Common carp, Koi carp (Cyprinus carpio carpio Linnaeus) Caucasian carp, Crucian carp (Carassius carassius Linnaeus) Iberian toothcarp, Spanish toothcarp (Aphanius iberus Valenciennes) Silver carp (Hypophthalmichthys molitrix Valenciennes) Catfish Catfish species (Ictalurus spp. Rafinesque) Black bullhead (Ameiurus melas – valid name), (Ictalurus melas Rafinesque – scientific name) Blue catfish (Ictalurus furcatus Valenciennes) 1. Aeromonas bestiarum 2. Atypical Aeromonas salmonicida 3. A. veronii ssp. veronii 4. Citrobacter freundii 5. Flavobacterium columnare 6. Yersinia ruckeri 7. Aeromonas eucrenophila 8. A. sobria were non-pathogenic to carp in virulence studies (452) 9. Streptococcus iniae and S. agalactiae (S. difficile) were non-pathogenic in virulence studies (234) 1, 3. Haemorrhage, necrosis, ulcers 2. Ulcers, lesions 3. Also bloody ascites fluid 4. In fingerlings 5. Gills 6. Can be infected with or without clinical signs 7. Ascites Escherichia vulneris Haemorrhage in eyes, swollen, dark coloured abdomen, yellow liver, yellow fluid in intestine 1, 3. Pathogenic on virulence studies 2. Ulcerating dermal lesions 4. Heavy mortalities, septicaemia, opportunistic infection 5. Disease 6. Yersiniosis 7. Non-pathogenic 135 209 234 271 379 425 452 473 760 Mortality 51 Vibrio parahaemolyticus External haemorrhage, tail rot Mortality 10 1. Providencia (Proteus) rettgeri 2. Staphylococcus aureus 1. Large red ulcerative lesions on the abdomen, base of the pectoral fin and on the head. Organism isolated from internal organs. Pond had been fertilized with poultry faeces from which P. rettgeri was isolated 2. Red cornea becoming opaque Degeneration of eye tissue 1. Mass mortality 2. Eye disease Flavobacterium columnare Organism in kidney 88 1. Edwardsiella ictaluri 2. Flavobacterium columnare 2. Yellow-edged skin erosion on head 1. Enteric septicaemia of catfish (ESC), edwardsiellosis 2. Columnaris disease Edwardsiella ictaluri ESC 334 79 688 88
Page 1 and 2: Bacteria from Fish and Other Aquati
Page 3 and 4: Contents List of Tables and Figures
Page 5 and 6: List of Tables and Figures Tables T
Page 7 and 8: Foreword While aquatic animal disea
Page 9 and 10: Acknowledgements xi Principal spons
Page 11 and 12: Introduction xiii Medical laborator
Page 13 and 14: Photographs of Culture and Microsco
Page 15 and 16: Fig. 11. Aeromonas veronii ssp. sob
Page 17 and 18: Fig. 22. Edwardsiella tarda on BA,
Page 19 and 20: Fig. 43. Listonella anguillarum, Gr
Page 21 and 22: Fig. 55. Plesiomonas shigelloides,
Page 23 and 24: Fig. 69. Vibrio alginolyticus, Gram
Page 25 and 26: Fig. 82. Vibrio harveyi on MSA-B, 4
Page 27 and 28: Fig. 98. Vibrio proteolyticus on BA
Page 29 and 30: Fig. 118. Methyl Red reaction. Posi
Page 31 and 32: 2 Chapter 1 Table 1.1. Host species
Page 33: 4 Chapter 1 Table 1.1. Continued. H
Page 37 and 38: 8 Chapter 1 Table 1.1. Continued. H
Page 39 and 40: 10 Chapter 1 Table 1.1. Continued.
Page 65 and 66: 36 Chapter 1 Bacterial gill disease
Page 67 and 68: 38 Chapter 1 Table 1.2. Bacterial p
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56 Chapter 1 Table 1.2. Continued.
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76 Chapter 1 of the test, or the us
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78 Chapter 1 in a similar manner to
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80 Chapter 1 phenotypic results rec
Page 111 and 112:
82 Chapter 1 formerly Y. enterocoli
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84 Chapter 2 2.1 Specimen Collectio
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86 Chapter 2 Table 2.3. Specific cu
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114 Chapter 2 reaction, oxidase and
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116 Chapter 2 API 20E API 20NE API
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118 Chapter 3 Table 3.1. Interpreta
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120 Chapter 3 to produce alkaline p
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122 Chapter 3 V. splendidus biovar
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124 Chapter 3 are likely to produce
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126 Chapter 3 Table 3.2. Carnobacte
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128 Chapter 3 reported a negative r
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130 Chapter 3 Table 3.5. Further di
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132 Chapter 3 to be due to the diff
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134 Chapter 3 control and a negativ
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136 Chapter 3 Yersinia ruckeri Stra
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138 Chapter 4 Fig. 4.1. Schematic f
Page 169 and 170:
140 Table 4.1. Aeromonas salmonicid
Page 171 and 172:
142 Table 4.3. Aeromonas spp. - mot
Page 173 and 174:
144 Table 4.5. Brucella spp. Test G
Page 175 and 176:
146 Table 4.8. Cytophaga-Flavobacte
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148 Table 4.8. Continued. Test Gm O
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150 Table 4.10. Continued. Test Gm
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160 Table 4.13. Gram-positive cocci
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164 Table 4.14. Gram-positive rods.
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166 Table 4.15. Mycobacterium and N
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168 Table 4.17. Mycoplasma spp. Tes
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178 Table 4.23. API 20E database bi
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180 Table 4.23. Continued. Tests on
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182 Table 4.23. Continued. Tests on
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184 Table 4.24. API 20E database nu
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186 Table 4.24. Continued. Organism
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190 Table 4.25. API 20E database nu
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192 Table 4.25. Continued. API 20 E
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198 Table 4.26. Continued. Listonel
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200 Table 4.27. API 50CH database b
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202 Table 4.27. Continued. 1 2 3 4
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208 Table 4.28. API Coryne database
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210 Table 4.30. API Rapid ID32 Stre
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212 Table 4.31. API ZYM database re
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214 Table 4.31. Continued. Well 1 2
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216 Table 4.31. Continued. Well 1 2
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218 Table 4.32. Strains quoted in r
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5 Technical Methods 5.1 Total Bacte
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224 Chapter 5 Incubation Place plat
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226 Chapter 6 Table 6.1. List of sp
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232 Chapter 6 Edwardsiella spp. PCR
Page 263 and 264:
234 Chapter 6 Extraction of DNA fro
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236 Chapter 6 The nucleotides can a
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238 Chapter 6 Table 6.3. Universal
Page 269 and 270:
240 Chapter 6 Visualize DNA on agar
Page 271 and 272:
242 Chapter 6 document with the seq
Page 273 and 274:
7 Preparation of Media for Culture
Page 275 and 276:
246 Chapter 7 Blood agar - BA Reage
Page 277 and 278:
248 Chapter 7 Edwardsiella ictaluri
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250 Chapter 7 Flexibacter maintenan
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252 Chapter 7 Marine 2216 agar (Dif
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254 Chapter 7 Mycoplasma medium - m
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256 Chapter 7 R2A agar (Oxoid CM 90
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258 Chapter 7 Siem selective medium
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260 Chapter 7 VVM Selective medium
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262 Chapter 7 Brucella: growth on s
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264 Chapter 7 Carbohydrate fermenta
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266 Chapter 7 Congo Red. Tests for
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268 Chapter 7 Furunculosis agar (Fo
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270 Chapter 7 Indoxyl acetate hydro
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272 Chapter 7 Glucose (10% w/v) 10
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274 Chapter 7 ONPG - o-nitrophenyl-
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276 Chapter 7 Salt - 20% stock solu
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Further Reading and Other Informati
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280 Further Reading List of Bacteri
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282 Appendix Common name Scientific
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288 Glossary C Ca Cat CBBA CCA CCRC
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290 Glossary O Oxidative (aerobic m
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References 1 Abbott, S.L., Cheung,
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294 References otherwise infected w
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296 References 87 Bergman, S., Seli
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298 References Journal of Systemati
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300 References ribonucleic acid seq
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302 References ‘winter disease’
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304 References 273 Fukuda, Y., Mats
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306 References resistance of virule
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308 References and an emended descr
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310 References the Enterobacteriace
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312 References an epizootic of cult
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314 References 512 Macián, M.C., L
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316 References Plecoglossus altivel
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318 References sponge. Internationa
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320 References fish pathogens. Dise
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322 References Listonella and reass
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324 References 750 Toranzo, A.E., B
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326 References 795 Vreeland, R.H.,
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328 References 842 Wood, R. (1965)
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330 Index Aeromonas continued A. ca
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332 Index API identification system
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334 Index Brucella agar 246 Brucell
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336 Index Colony forming units (CFU
Page 367 and 368:
338 Index Dolphin continued Striped
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340 Index Flavobacterium species co
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342 Index Granulicatella continued
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344 Index Laminaria 59 Laminaria ja
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346 Index Mycobacterium continued M
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348 Index Peled see Whitefish Pelec
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350 Index Pseudoalteromonas continu
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352 Index Salmonella continued S. j
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354 Index Shewanella marine agar (S
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356 Index Strontium chloride B enri
Page 387 and 388:
358 Index Vibrio continued V. brasi
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360 Index Vibrio continued V. scoph
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