B.Sc. - Acharya Nagarjuna University

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Principle and applications of centrifugation techniques differential, density gradient. Ultracentrifugation preparative and analytical.. Principle and applications of chromatographic techniques paper, thin layer, gel filtration, ion exchange and affinity chromatography. Elementary treatment of an enzyme purification. Electrophoresis principles and applications of paper, polyacrylamide (native and SDS) and agarose gel electrophoresis. Colorimetry and Spectrophotometry Laws of light absorption BeerLambert law. UV and visible absorption spectra, molar extinction coefficient, biochemical applications of spectrophotometer. Principle of fluorimetry. Tracer techniques: Radio isotopes, units of radio activity, half life, β and γ emitters, use of radioactive isotopes in biology.
2 nd Year Practical – PaperII: Quantitative Analysis and Biochemical Techniques 90 Hrs (3hrs/week) List of Experiments: 1. Estimation of amino acid by ninhydrin method. 2. Estimation of protein by Biuret method. 3. Estimation of protein by Lowry method. 4. Estimation of glucose by DNS method. 5. Estimation of glucose by Benedict’s titrimetric method. 6. Estimation of total carbohydrates by anthrone method. 7. Isolation of egg albumin from egg white. 8. Isolation of cholesterol from egg yolk. 9. Isolation of starch from potatoes. 10.Isolation of casein from milk. 11.Separation of amino acids by paper chromatography. 12.Determination of exchange capacity of resin by titrimetry. 13.Separation of serum proteins by paper electrophoresis. 14.Separation of plant pigments by TLC.
Page 1 and 2: MODIFIED CURRICULUM B.Sc. From the
Page 3 and 4: Second Year ENGLISH Syllabus PAPER
Page 5 and 6: The Pattern of the Question Paper f
Page 7 and 8: 8) Grammar Declensions : Pronouns :
Page 9 and 10: Second Year HINDI (Second Language)
Page 11 and 12: ENVIRONMENTAL STUDIES COURSE METERI
Page 13 and 14: ►Food resources ▪ World food pr
Page 15 and 16: Module 11: Human Population and the
Page 17 and 18: For all B.A./B.Sc./B.Com. Programs
Page 19 and 20: General Stream B.A./B.Com./B.Sc.: I
Page 21 and 22: Model Question Paper General Stream
Page 23 and 24: Second Year BOTANY Syllabus Paper
Page 25 and 26: of modern medicine: Aswagandha (Wit
Page 27 and 28: Practical II: Anatomy, Embryolog
Page 29: Second Year BIOCHEMISTRY Syllabus
Page 33 and 34: 3.3 Disease causing pathogens and t
Page 35 and 36: Second Year CHEMISTRY SYLLABUS Pape
Page 37 and 38: Oxidation of aldehydes: BaeyerVil
Page 39 and 40: hydrolysis of esters, mutarotation
Page 41 and 42: I. Titrimetric analysis: Second Yea
Page 43 and 44: Class , Objects and Methods: Defini
Page 45 and 46: B.Sc(Computer Science): II Year: La
Page 47 and 48: Model Question Paper B.Sc.(Computer
Page 49 and 50: B.Sc II Year Electronics Syllabus
Page 51 and 52: B.Sc II Year Electronics PRACTIC
Page 53 and 54: ,Properly divergent sequences, Intr
Page 55 and 56: Brief account on horizontal gene tr
Page 57 and 58: Second Year MICROBIOLOGY LAB - II:
Page 59 and 60: theory of radiation Planck’s l
Page 61 and 62: NOTE: Problems should be solved at
Page 63 and 64: B .A/B.Sc. I I Year: Statistics Syl
Page 65 and 66: B.A/B.Sc. II Year: Statistics Sylla
Page 67 and 68: 2.2.2. Migration in birds 2 hours 2
Page 69: REFERENCE BOOKS 1. ‘Chordate Zool

B.Sc. - Acharya Nagarjuna University

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