T. reesei - TNAU Genomics
T. reesei - TNAU Genomics
T. reesei - TNAU Genomics
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2-DE analysis of the purified 26S proteasome<br />
q first dimension by pI<br />
q second dimension by mass<br />
q spots analysed by tandem mass spectrometry<br />
protein mass<br />
3 pI 10<br />
Protein spot from<br />
2-D gel<br />
Tryptic digestion &<br />
peptide extraction<br />
TYGGAAR EHICLLGR<br />
GPGFK<br />
GANR PSTTGVEMFR<br />
Unmodified and<br />
modified peptides<br />
Protein identification<br />
by database matching<br />
624.3<br />
769.8<br />
893.4<br />
994.5<br />
1056.1<br />
1326.7<br />
1501.9<br />
1759.8<br />
1923.4<br />
2100.6<br />
600 2200<br />
MALDI mass<br />
spectrometry<br />
or MS/MS<br />
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