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8 1 Cell Analysis Bioluminescent Cell Viability Kit I (ATP) Bioluminescent Cell Viability Kit II (ADP/ATP) Cell Proliferation Kit I (CFSE) PromoKine’s Cell Proliferation Kit I The Promokine Bioluminescent Cell The ADP/ATP ratio is a good indicator for provides a convenient and sensitive 1 Cell Analysis Viability Kit I employs bioluminescence to measure the ATP level as a marker for cell viability in mammalian cells. It utilizes luciferase to catalyze the ATP dependent, cell viability. High ATP and low ADP levels are usually found in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are characteristic for way to determine cell proliferation or cell division. The assay is based on the amine-reactive dye CFDA SE (5-(and 6)-carboxyfluorescein diacetate, succin- light-producing oxidation of luciferin. apoptotic cells. In necrotic cells, the ADP imidyl ester; also known as CFSE) which This light formation can be detected us- levels are even higher, and ATP can be is non-fluorescent and can diffuse pas- ing a luminometer or beta counter. barely detected. The Bioluminescent Cell sively into cells. When it enters the cells, The assay can be performed directly in Viability Kit II utilizes bioluminescence to the acetate group is removed by intracel- culture plates requiring no harvesting, measure the ADP/ATP ratio providing an lular esterases yielding the bright green- washing, or sample preparation and can innovative system for fast, simultaneous fluorescent dye carboxyfluorescein SE. be fully automated for high throughput screening of cell proliferation, apoptosis, Its succinimidyl ester (SE) reactive group (10 seconds/sample). It is highly sensi- necrosis, and growth arrest in mammalian reacts with intracellular amines and at- tive, detecting 10-100 mammalian cells cells. The ATP level is determined using taches the fluorescent dye to intracellular per well. It also offers applications be- the luciferin-luciferase reaction used in proteins, retaining it in the cells and al- sides cell viability testing, e.g., detect- the Bioluminescent Cell Viability Kit I. The lowing fixation with aldehyde fixatives. ing low level bacterial contamination in ADP level is measured by its conversion CFDA SE has been used as a tracer for blood, milk, urine, soil, and sludge. to ATP, which is subsequently detected several cell divisions as the label is stably using the same luciferase-catalyzed re- inherited by daughter cells through suc- action. The assay works with full auto- cessive cell divisions or after cell fusion mation for high throughput, detects as and is not transferred to adjacent cells in few as 100 mammalian cells per well, a population. and offers highly consistent results with The kit is compatible with flow cytometry excellent correlation to other apoptosis and microscopy and contains 10 single- markers (e.g., TUNEL-based or caspase use vials as well as a detailed protocol for assays). cellular labeling. Product Size Catalog Number Fluorometric Cell Viability Kit I (Resazurin) 500 assays 2500 assays 10000 assays PK-CA707-30025-0 PK-CA707-30025-1 PK-CA707-30025-2 Fluorometric Cell Viability Kit II (Calcein) 1000 assays PK-CA707-30026 Colorimetric Cell Viability Kit I (WST-8) Colorimetric Cell Viability Kit II (WST-1) Colorimetric Cell Viability Kit III (XTT) Colorimetric Cell Viability Kit IV (MTT) Bioluminescent Cell Viability Kit I (ATP) 100 assays 500 assays 500 assays 2500 assays 200 assays 1000 assays 200 assays 1000 assays 200 assays 1000 assays PK-CA705-CK04-100 PK-CA705-CK04 PK-CA577-K301-500 PK-CA577-K301-2500 PK-CA20-300-200 PK-CA20-300-1000 PK-CA707-30006-200 PK-CA707-30006 PK-CA577-K254-200 PK-CA577-K254-1000 Bioluminescent Cell Viability Kit II (ADP/ATP) 200 assays PK-CA577-K255 Cell Proliferation Kit I (CFSE) 10 x 50 µg PK-CA707-30050 Resazurin, sodium salt 10 mg PK-CA707-10054 For prices please see our online shop at www.promokine.info PromoKine also provides high-quality tetrazolium salts for preparing custom cell assays including INT, MTT, XTT, and various WST salts such as WST-8. Please visit our website to find the complete range of tetrazolium salts.
1 Cell Analysis 9 1.2 Live/Dead Cell Staining Kits Applications Ethidium homodimer III (EthD-III). Cal- Bacteria Live/Dead Staining Kit cein-AM penetrates the cell membrane Monitoring of viable and dead cells of living cells. Once inside, the cellular The Bacteria Live/Dead Staining Kit en- Cell counting by fluorescence micros- esterases present in living cells hydrolyze ables a two-color fluorescent staining copy Features it to cell membrane-impermeable, greenfluorescent Calcein (emission maximum at 515 nm). In contrast, EthD-III can only pass on both live bacteria (green) and dead bacteria (red) using the two nucleic acid dyes DMAO and EthD-III. DMAO is a green-fluorescent dye which stains 1 Cell Analysis Simultaneous monitoring of viable through the disrupted membrane areas both live and dead bacteria with intact and dead cells of dead cells where it intercalates with and damaged cell membranes while High fluorescent intensity the DNA of the nucleus, emitting red the red-fluorescing EthD-III only stains Fast and convenient procedure light at 620 nm. Compared with PI, dead bacteria with damaged cell mem- Excitation and emission spectra are EthD-III is more stable and emits brighter branes. With an appropriate mixture of compatible with common excitation fluorescent signals. Since both Calcein both dyes, viable bacteria with intact cell sources and filter sets and EthD-III can be excited at 490 nm, membranes are stained green, whereas Live/Dead Cell Staining Kit II a simultaneous monitoring of viable and dead cells is possible using a fluorescence bacteria with damaged cell membranes fluoresce red. The kit is applicable to most microscope. At an excitation of 545 nm, bacteria types for viability and cytotoxic- The Live/Dead Cell Staining Kit II utilizes the number of dead cells can be moni- ity studies, and suitable for fluorescence two fluorescent dyes, Calcein-AM and tored. microscopy and flow cytometry. Calcein-AM R: acetoxymethyl EthD-III Esterase Viable cell DNA intercalation red fluorescence Fig. 4: Double-staining of viable Calcein green fluorescence (green) and dead (red) cells using the PromoKine Live/Dead Cell Staining Kit II. Fig. 3: Assay mechanism of the PromoKine Live/Dead Cell Staining Kit II. Product Size Catalog Number Live/Dead Cell Staining Kit II 300-1000 assays PK-CA707-30002 Bacteria Live/Dead Staining Kit 100-1000 assays PK-CA707-30027 For prices please see our online shop at www.promokine.info Please see also the PromoKine Apoptotic/Necrotic/Healthy Cells Detection Kit in Chapter 2 and our individual stains for viable and dead cells in Chapter 1.6.
Page 1 and 2: Cell Biology Products 2011 | 2012
Page 3 and 4: Overview 1 Overview 1 Cell Analysis
Page 5: Contents 3 4 5 6 7 8 Gene Cloning,
Page 8 and 9: 6 Contents Chapter 1 1 Cell Analysi
Page 12 and 13: 10 1 Cell Analysis 1.3 Cytotoxicity
Page 14 and 15: 12 1 Cell Analysis Product Size Cat
Page 16 and 17: 14 1 Cell Analysis Product Product
Page 26 and 27: 24 1 Cell Analysis 1.9 Cell Stress
Page 28 and 29: 26 1 Cell Analysis 1.10 Cell Fracti
Page 30 and 31: 28 1 Cell Analysis 1.11 Nucleic Aci
Page 33 and 34: Apoptosis 2 Apoptosis, or programme
Page 35 and 36: 2 Apoptosis 33 2.1 DNA Fragmentatio
Page 37 and 38: 2 Apoptosis 35 2.4 Glutathione Dete
Page 39 and 40: 2 Apoptosis 37 Product Size Catalog
Page 41 and 42: 2 Apoptosis 39 Caspase Screening Ki
Page 43 and 44: 2 Apoptosis 41 Product Size Catalog
Page 45 and 46: 2 Apoptosis 43 2.8 Kinase Detection
Page 47 and 48: 2 Apoptosis 45 2.10 Apoptotic Cell
Page 49 and 50: Fluorescent Labeling 3 The availabi
Page 51 and 52: 3 Fluorescent Labeling 49 3.1 Promo
Page 53 and 54: 3 Fluorescent Labeling 51 Excitatio
Page 55 and 56: 3 Fluorescent Labeling 53 3.2.2 Pro
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3 Fluorescent Labeling 59 3.5 Phyco
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Gene Cloning, Expression, 4 and Sil
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4 Gene Cloning, Expression, and Sil
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74 Contents Chapter 5 5 Cell Transf
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76 5 Cell Transfection Fig. 4: Comp
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78 5 Cell Transfection n Some cell
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80 5 Cell Transfection Phase contra
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82 5 Cell Transfection Magnet Plate
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Antibodies and ELISAs 6 Antibodies
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6 Antibodies and ELISAs 87 6.1 Mono
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6 Antibodies and ELISAs 89 n Kinase
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6 Antibodies and ELISAs 91 6.2 Labe
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6 Antibodies and ELISAs 93 6.3 ELIS
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6 Antibodies and ELISAs 95 Product
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Cytokines and Growth 7 Factors Cyto
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138 Contents Chapter 8 8 Microbial
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140 8 Microbial Detection and Elimi
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142 8 Microbial Detection and Elimi
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144 Alphabetical Index Alphabetical
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146 Alphabetical Index Description
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160 Alphabetical International Inde
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162 International Alphabetical Inde
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Notes Notes 165
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Terms and Conditions 167 4. The rig
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Fax Order Form Shipping Address Inv
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